##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 258219 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968166556295238 33.0 31.0 34.0 31.0 34.0 2 32.03987313094699 34.0 31.0 34.0 30.0 34.0 3 32.02691126524385 34.0 31.0 34.0 30.0 34.0 4 35.55446345931167 37.0 35.0 37.0 35.0 37.0 5 35.61828525398983 37.0 35.0 37.0 35.0 37.0 6 35.745289850863024 37.0 36.0 37.0 35.0 37.0 7 35.70535088432687 37.0 36.0 37.0 35.0 37.0 8 35.71050542369074 37.0 36.0 37.0 35.0 37.0 9 37.33516511178496 39.0 38.0 39.0 35.0 39.0 10-11 37.29054600939513 39.0 37.0 39.0 35.0 39.0 12-13 37.225055088897406 39.0 37.0 39.0 35.0 39.0 14-15 38.39241496559123 40.0 38.0 41.0 34.0 41.0 16-17 38.337564238107966 40.0 38.0 41.0 34.0 41.0 18-19 38.353335734396 40.0 38.0 41.0 34.0 41.0 20-21 38.32016427915839 40.0 38.0 41.0 34.0 41.0 22-23 38.2149512623006 40.0 38.0 41.0 34.0 41.0 24-25 38.16183743256693 40.0 38.0 41.0 34.0 41.0 26-27 38.01702237248227 40.0 38.0 41.0 34.0 41.0 28-29 37.837736572444314 40.0 37.0 41.0 34.0 41.0 30-31 37.535617053741205 40.0 36.5 41.0 33.5 41.0 32-33 37.178433810060454 40.0 35.0 41.0 33.0 41.0 34-35 36.83107749623382 39.0 35.0 41.0 33.0 41.0 36-37 36.53130094996882 39.0 35.0 41.0 32.0 41.0 38-39 36.17217168372583 38.5 35.0 41.0 30.5 41.0 40-41 36.01459613738726 39.0 35.0 41.0 31.0 41.0 42-43 35.752940333592804 39.0 35.0 41.0 30.0 41.0 44-45 35.41316673056592 39.0 35.0 41.0 28.0 41.0 46-47 34.97881643101398 39.0 35.0 41.0 24.0 41.0 48-49 34.640452484131686 39.0 35.0 41.0 22.0 41.0 50-51 33.47458746258021 38.0 34.0 40.0 17.5 40.5 52-53 33.60305206046031 38.0 34.0 39.5 15.0 40.5 54-55 34.07847214960944 39.0 35.0 40.5 11.0 41.0 56-57 33.99066296438295 39.0 35.0 41.0 4.5 41.0 58-59 33.80914069065406 38.5 35.0 41.0 2.0 41.0 60-61 33.53581262416786 38.0 35.0 40.5 2.0 41.0 62-63 33.16589019398263 38.0 34.0 40.0 2.0 41.0 64-65 32.76706594015158 37.0 34.0 40.0 2.0 41.0 66-67 32.38230532997184 37.0 33.5 39.5 2.0 41.0 68-69 31.937365182267765 36.0 33.0 39.0 2.0 41.0 70-71 31.48326614230556 36.0 33.0 39.0 2.0 40.5 72-73 30.995953047606875 35.0 32.0 38.0 2.0 40.0 74-75 30.51141279301678 35.0 31.5 37.0 2.0 39.5 76-77 30.03003845572944 35.0 31.0 37.0 2.0 39.0 78-79 29.56911962326552 35.0 31.0 36.0 2.0 39.0 80-81 29.06951076411883 35.0 30.0 36.0 2.0 37.5 82-83 28.58315615814483 35.0 29.0 35.5 2.0 37.0 84-85 28.133686909173996 34.5 29.0 35.0 2.0 37.0 86-87 27.815360217489804 34.0 29.0 35.0 2.0 36.0 88-89 27.3548092897889 34.0 27.0 35.0 2.0 36.0 90-91 26.92439169851947 34.0 26.0 35.0 2.0 36.0 92-93 26.523154763979413 34.0 25.0 35.0 2.0 35.0 94-95 26.04959937107649 34.0 23.0 35.0 2.0 35.0 96-97 25.51641823413459 34.0 17.0 35.0 2.0 35.0 98-99 24.926717243889875 33.5 2.0 35.0 2.0 35.0 100-101 22.88766705780752 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28417811082648825 1101 2 0.24373579266616474 1101 3 0.30125058871717414 1101 4 0.3955997573978749 1101 5 0.4332439064630478 1101 6 0.4630506426682146 1101 7 0.47223131786297756 1101 8 0.34736502848737416 1101 9 0.529209816350459 1101 10-11 0.4688451063105106 1101 12-13 0.5518133091560387 1101 14-15 0.5311841291462045 1101 16-17 0.8249226209982723 1101 18-19 0.8652820681920801 1101 20-21 0.8247156118657983 1101 22-23 0.9846532216122839 1101 24-25 0.916159160234173 1101 26-27 0.684357784001584 1101 28-29 0.7029153423672341 1101 30-31 0.7048214180605825 1101 32-33 0.5699982518039164 1101 34-35 0.26798172128889775 1101 36-37 0.07458428837579589 1101 38-39 0.27063943850012606 1101 40-41 0.20646122952334878 1101 42-43 0.24093838354668407 1101 44-45 0.4822825822207335 1101 46-47 0.3915950201447558 1101 48-49 0.07073407766404216 1101 50-51 0.034309545108882844 1101 52-53 0.08875383713522922 1101 54-55 0.09819934679643438 1101 56-57 0.018041025989894877 1101 58-59 -0.06798451080137369 1101 60-61 -0.02114588082965696 1101 62-63 0.026052078217276176 1101 64-65 0.1073745173179006 1101 66-67 0.12432653842021324 1101 68-69 0.052446428341053064 1101 70-71 0.09980134505894966 1101 72-73 0.19471049664355178 1101 74-75 -0.2602786716797283 1101 76-77 -7.774707656480473E-4 1101 78-79 0.052303781918091374 1101 80-81 -0.11456431318910276 1101 82-83 0.09905584896455011 1101 84-85 0.3180322509306315 1101 86-87 0.3808495063670492 1101 88-89 0.6053483526304042 1101 90-91 0.5148899465533354 1101 92-93 0.419577005683049 1101 94-95 0.682072479174682 1101 96-97 0.9327656471644801 1101 98-99 0.9028653408444036 1101 100-101 0.6483450682591076 1102 1 0.3113503067190635 1102 2 0.14164258571513955 1102 3 0.49512894574719013 1102 4 0.1504575773030865 1102 5 0.03482368529400759 1102 6 0.22971730933488033 1102 7 0.1798932451931563 1102 8 0.4943831959439322 1102 9 0.30162292851475314 1102 10-11 0.31016619635789766 1102 12-13 0.370869391304538 1102 14-15 0.2878389474716414 1102 16-17 0.1853886557534068 1102 18-19 0.1810601092663262 1102 20-21 0.5557878867473178 1102 22-23 0.6092187824337643 1102 24-25 0.4900039469640234 1102 26-27 0.29936568289573984 1102 28-29 0.43123287791225096 1102 30-31 0.4240650989452561 1102 32-33 0.31731262779128144 1102 34-35 0.3763545490930085 1102 36-37 0.5753840514089745 1102 38-39 0.6956690593532073 1102 40-41 1.097831687659209 1102 42-43 0.7455987310980277 1102 44-45 0.7707600703723898 1102 46-47 0.9100883060847238 1102 48-49 1.0878055626561363 1102 50-51 1.1150066225180524 1102 52-53 1.148193015650243 1102 54-55 0.7513589044583568 1102 56-57 0.9273419738571889 1102 58-59 1.1201177798779298 1102 60-61 1.2089192850471235 1102 62-63 1.01807419512091 1102 64-65 1.2235475030998941 1102 66-67 1.1649762224644533 1102 68-69 0.8338642798418441 1102 70-71 0.670575436686125 1102 72-73 0.7158202912723013 1102 74-75 0.985712639536704 1102 76-77 0.8673801121727394 1102 78-79 0.3964487266258345 1102 80-81 0.5099123850731395 1102 82-83 0.4874642217923544 1102 84-85 0.40107924935085393 1102 86-87 0.586220754392329 1102 88-89 0.8951291583176051 1102 90-91 0.9312011629830366 1102 92-93 0.5235067055250688 1102 94-95 0.9600148172473517 1102 96-97 0.7068967688074501 1102 98-99 0.9413921970845323 1102 100-101 0.6708964110711158 1103 1 0.2776011302584749 1103 2 0.24354894662729976 1103 3 0.08514446016710053 1103 4 0.11566702197186629 1103 5 0.1558149079578115 1103 6 0.18038995507478717 1103 7 -0.26715582712954244 1103 8 -0.016891324277942488 1103 9 0.0862912812234029 1103 10-11 0.0338395009293464 1103 12-13 0.09518027477636792 1103 14-15 0.08501447294291609 1103 16-17 0.285722322044613 1103 18-19 0.19343976624887915 1103 20-21 0.08292749527387144 1103 22-23 0.09758296750167261 1103 24-25 0.25161319610860744 1103 26-27 0.3030423878879773 1103 28-29 0.040471396178887176 1103 30-31 -0.06362962827724061 1103 32-33 0.3833764281865797 1103 34-35 0.43285466598246813 1103 36-37 0.21558765160450122 1103 38-39 0.293537420562906 1103 40-41 0.30748888273709696 1103 42-43 0.14803853302351655 1103 44-45 0.09682854634629479 1103 46-47 0.05965368980096031 1103 48-49 0.09574341996598434 1103 50-51 -0.21411160586271194 1103 52-53 -0.2630772540456334 1103 54-55 -0.17706223765797802 1103 56-57 -0.2545486601087603 1103 58-59 -0.37643929405997056 1103 60-61 -0.19684655347539604 1103 62-63 -0.4577296856093298 1103 64-65 -0.127322792099136 1103 66-67 -0.21759237039891843 1103 68-69 -0.38284878840035574 1103 70-71 -0.12157384178708952 1103 72-73 -0.01199727615166779 1103 74-75 -0.3846994489592461 1103 76-77 -0.2581485469988323 1103 78-79 -0.6730792524615765 1103 80-81 -0.5038486928602524 1103 82-83 -0.4798492332477089 1103 84-85 -0.836461396304049 1103 86-87 -0.8104808374296582 1103 88-89 -1.03537474154 1103 90-91 -1.061604821757573 1103 92-93 -0.9948661931211369 1103 94-95 -1.2040448601377243 1103 96-97 -0.8724286727159374 1103 98-99 -0.5559724767938583 1103 100-101 -0.5899583697080075 1104 1 0.2902387168870959 1104 2 0.3157054896358673 1104 3 0.16737612984271522 1104 4 -0.08156474476663078 1104 5 -0.061994188775052805 1104 6 -0.04300996339239305 1104 7 0.15469885033051156 1104 8 0.10899922762157388 1104 9 -0.03761918797854946 1104 10-11 -0.08897415775875572 1104 12-13 0.11128300612573128 1104 14-15 0.16208239754447362 1104 16-17 0.1678879889636491 1104 18-19 0.12161323702324722 1104 20-21 0.2408682092683918 1104 22-23 0.3783761653352258 1104 24-25 0.2449740952991064 1104 26-27 0.13500713465093384 1104 28-29 0.15172486617674963 1104 30-31 0.11269479468396071 1104 32-33 0.17540950721517845 1104 34-35 0.38789514120232127 1104 36-37 0.3745572320987449 1104 38-39 0.7860344601451459 1104 40-41 0.540498026060142 1104 42-43 0.3130163056246076 1104 44-45 0.1229481666363128 1104 46-47 0.16083203313177563 1104 48-49 -0.06176051108054281 1104 50-51 -0.1266482470989061 1104 52-53 0.28734691072831 1104 54-55 0.025255623852707743 1104 56-57 0.3215583420072079 1104 58-59 0.138644277077411 1104 60-61 0.42763658670281046 1104 62-63 0.5137144158796119 1104 64-65 0.5111082835516711 1104 66-67 0.24064147348514808 1104 68-69 0.3812884196830417 1104 70-71 0.3686325138901161 1104 72-73 0.2511498905829441 1104 74-75 -0.20838473228578636 1104 76-77 -0.09144305518123375 1104 78-79 -0.24355660769229814 1104 80-81 -0.4379138802886686 1104 82-83 -0.11723202982333092 1104 84-85 -0.2978497837014018 1104 86-87 -0.14079551527797207 1104 88-89 -0.27011160407955614 1104 90-91 -0.042794036130647584 1104 92-93 -0.024091825485779594 1104 94-95 0.245113604715808 1104 96-97 0.18676997616880797 1104 98-99 0.23062508110414726 1104 100-101 0.15018489509893485 1105 1 0.4633447774931554 1105 2 0.41040245933283614 1105 3 0.247083922050507 1105 4 0.6122664240645435 1105 5 0.2874105731297121 1105 6 0.38805064266821176 1105 7 0.32639798452964897 1105 8 0.4265316951540399 1105 9 0.5833764830171262 1105 10-11 0.16676177297717487 1105 12-13 0.3934799758227001 1105 14-15 0.5832674624795402 1105 16-17 0.2790892876649451 1105 18-19 0.32778206819207867 1105 20-21 0.33513227853246264 1105 22-23 0.47423655494561245 1105 24-25 0.322409160234173 1105 26-27 0.2989411173349126 1105 28-29 0.41124867570056267 1105 30-31 0.4027380847272468 1105 32-33 0.32208158513724783 1105 34-35 0.5179817212888977 1105 36-37 0.7787509550424616 1105 38-39 0.8685561051667889 1105 40-41 1.0668778961900145 1105 42-43 0.8638550502133526 1105 44-45 1.1114492488874035 1105 46-47 1.0228450201447572 1105 48-49 0.8874007443307121 1105 50-51 0.7343095451088857 1105 52-53 0.703337170468572 1105 54-55 0.685699346796433 1105 56-57 0.6367910259898935 1105 58-59 0.9507654891986235 1105 60-61 0.8017707858370144 1105 62-63 0.9781354115506176 1105 64-65 1.0115411839845692 1105 66-67 1.0451598717535475 1105 68-69 1.0295297616743788 1105 70-71 1.1227180117256204 1105 72-73 1.3530438299768832 1105 74-75 1.5626379949869431 1105 76-77 1.424222529234349 1105 78-79 1.208553781918095 1105 80-81 1.250019020144233 1105 82-83 1.419889182297883 1105 84-85 1.6805322509306322 1105 86-87 1.3329328397003835 1105 88-89 1.5720150192970692 1105 90-91 1.6044732798866725 1105 92-93 1.8154103390163847 1105 94-95 1.3883224791746862 1105 96-97 1.2098489804978136 1105 98-99 0.81744867417774 1105 100-101 0.20667840159243767 1106 1 0.24108867993218297 1106 2 0.17564636177186088 1106 3 0.10592538546514163 1106 4 0.32303878178812084 1106 5 0.13080488207280183 1106 6 -0.01825017033991827 1106 7 0.13849148046460868 1106 8 0.10092193905648372 1106 9 0.22280737732606326 1106 10-11 0.13444469980643703 1106 12-13 0.17041087013164713 1106 14-15 0.26563534865839955 1106 16-17 0.24301205189258468 1106 18-19 0.3044080844522412 1106 20-21 0.403628213491821 1106 22-23 0.5464418394984634 1106 24-25 0.6127546886894564 1106 26-27 0.34736591408287865 1106 28-29 0.2701917651314574 1106 30-31 0.24216897903619383 1106 32-33 0.19845353635676588 1106 34-35 0.3439979814514942 1106 36-37 0.3251432314652263 1106 38-39 0.7359341539472766 1106 40-41 0.6748555384664385 1106 42-43 0.3746782209450643 1106 44-45 0.06495534644837875 1106 46-47 0.20475558925044623 1106 48-49 0.3969027768510287 1106 50-51 0.4216571060844956 1106 52-53 0.26050180461491124 1106 54-55 0.569337558178546 1106 56-57 0.7738337089167189 1106 58-59 0.6170256518002546 1106 60-61 0.6247382655118088 1106 62-63 0.8061333790302925 1106 64-65 0.8993460620333522 1106 66-67 0.6206171888267207 1106 68-69 0.6338488673654403 1106 70-71 0.5189070361158592 1106 72-73 0.3145275698142811 1106 74-75 0.006692873035721192 1106 76-77 0.24297252923435053 1106 78-79 0.3696310176904518 1106 80-81 0.5133320282743128 1106 82-83 0.6294420278263395 1106 84-85 0.730684689955023 1106 86-87 0.5942641405133955 1106 88-89 0.7638341249881258 1106 90-91 0.8463940115939863 1106 92-93 0.567239607309066 1106 94-95 0.6785155686055795 1106 96-97 0.9614241837498447 1106 98-99 1.0533734709257097 1106 100-101 0.9859466942753663 1107 1 -0.030488555840175735 1107 2 0.16006912599949885 1107 3 0.04358392205050876 1107 4 0.06859975739787672 1107 5 0.11857723979638024 1107 6 0.09371730933487754 1107 7 0.1688979845296501 1107 8 0.131198361820708 1107 9 0.22937648301711988 1107 10-11 0.1270951063105059 1107 12-13 0.2756466424893702 1107 14-15 0.4383507958128732 1107 16-17 0.41325595433161055 1107 18-19 0.3414487348587443 1107 20-21 0.4894656118658034 1107 22-23 0.19198655494561478 1107 24-25 0.12590916023417265 1107 26-27 0.03702445066824822 1107 28-29 -0.08808465763276274 1107 30-31 0.10273808472724966 1107 32-33 0.30733158513725556 1107 34-35 0.24064838795555943 1107 36-37 0.1648342883757934 1107 38-39 0.3283894385001247 1107 40-41 0.2646278961900137 1107 42-43 0.10627171688002335 1107 44-45 -0.015467417779269965 1107 46-47 0.14917835347809216 1107 48-49 0.1003174109973699 1107 50-51 -0.19110712155777776 1107 52-53 0.29642050380189744 1107 54-55 0.3168660134630983 1107 56-57 -0.07045897401010848 1107 58-59 -0.15990117746803634 1107 60-61 -0.1758958808296498 1107 62-63 -0.04328125511605663 1107 64-65 0.13087451731789912 1107 66-67 -0.3850901282464534 1107 68-69 -0.08138690499228218 1107 70-71 -0.2488653216077168 1107 72-73 -0.31612283668978236 1107 74-75 0.04888799498694141 1107 76-77 0.1989725292343536 1107 78-79 0.567387115251428 1107 80-81 0.7045190201442324 1107 82-83 0.9302225156312147 1107 84-85 0.7234489175972989 1107 86-87 0.5721828397003854 1107 88-89 0.4345983526303989 1107 90-91 0.4145566132200038 1107 92-93 0.5337436723497184 1107 94-95 0.6469058125080167 1107 96-97 0.47043231383114303 1107 98-99 0.6270320075110725 1107 100-101 0.8686784015924438 1108 1 -0.3234847244225527 1108 2 -0.2241760847284695 1108 3 -0.13237010093800095 1108 4 -0.02384468704656939 1108 5 -0.43035762610400496 1108 6 -0.46760069832795637 1108 7 -0.35094875876536946 1108 8 -0.44645681059308373 1108 9 -0.2363553177491582 1108 10-11 -0.10076696265500829 1108 12-13 -0.1757250050202117 1108 14-15 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72-73 -0.15912937263749427 2316 74-75 -0.16575252788887695 2316 76-77 -0.0968967518113999 2316 78-79 -0.13662595664399646 2316 80-81 0.042584379621356305 2316 82-83 0.158614672493961 2316 84-85 -0.3204072915530283 2316 86-87 -0.20084984003817397 2316 88-89 -0.27091798723887806 2316 90-91 -0.7153388116165971 2316 92-93 -0.41677920346727504 2316 94-95 -0.48663667115211595 2316 96-97 -0.3285088626394419 2316 98-99 -0.49492877680265224 2316 100-101 0.08478297675583946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2011.0 3 352.0 4 254.0 5 262.0 6 266.0 7 298.0 8 359.0 9 402.0 10 537.0 11 682.0 12 1106.0 13 1850.0 14 1944.0 15 2338.0 16 3297.0 17 4593.0 18 3524.0 19 2085.0 20 2008.0 21 1874.0 22 1958.0 23 2175.0 24 2347.0 25 2621.0 26 2810.0 27 3220.0 28 3676.0 29 4165.0 30 5072.0 31 5928.0 32 7270.0 33 9537.0 34 13583.0 35 25476.0 36 27618.0 37 45476.0 38 55528.0 39 9717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26720907202082 24.71193615410008 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32.67736903804605 36.46201691581813 15.70923394402085 20-21 16.72906106124727 31.34407602040025 35.90099831031329 16.02586460803919 22-23 16.798057432105082 31.212864667511596 35.28171497893484 16.70736292144848 24-25 16.404476961093074 32.17558990261156 35.936624836474635 15.483308299820727 26-27 16.29290245306386 31.521201937333203 36.83685196921508 15.349043640387855 28-29 15.249405531320312 31.747437534762124 37.482049662116985 15.521107271800577 30-31 15.858014080767719 32.37917689715381 36.36058857841608 15.402220443662396 32-33 15.439378378902088 33.0245896874455 35.8988121766427 15.637219757009706 34-35 16.048833981929874 32.152596488844466 35.78350785594363 16.015061673282027 36-37 16.826632516435446 31.535264065108272 35.58976192650834 16.048341491947944 38-39 16.092418525671164 32.344929687469644 35.748258215433395 15.814393571425796 40-41 15.15864968083577 32.5708746615072 36.86162297827041 15.408852679386614 42-43 15.300277837144687 32.72086110085682 35.60978453049408 16.3690765315044 44-45 15.874194087448556 32.71412304667006 34.94017107303443 16.471511792846954 46-47 16.277040629931054 32.74801980582826 33.89591057839548 17.079028985845206 48-49 16.20049519382135 32.42275714902069 34.37729571311409 16.999451944043876 50-51 16.171020694506794 32.75072340408993 34.150697363486024 16.927558537917257 52-53 15.883081120683121 33.40556066221124 34.35993307150117 16.351425145604466 54-55 15.836251219294189 32.54592512853595 34.19697576179169 17.420847890378166 56-57 16.429433145605756 33.63146985058854 33.10927765355423 16.82981935025148 58-59 16.139534341625257 32.19670628091018 33.94263380976616 17.721125567698397 60-61 16.299006044507614 32.59529371103254 33.735070765203986 17.370629479255864 62-63 16.87756581005283 32.04773256706321 34.09474627016462 16.979955352719344 64-65 16.617854262420927 31.96684513803189 34.33588246154444 17.07941813800274 66-67 15.960818991905313 32.469122605848 33.603156247874296 17.966902154372395 68-69 15.84850281360248 32.12307931540532 34.05576667023024 17.97265120076196 70-71 16.33622566399204 32.47885177582883 33.61945636623748 17.565466193941656 72-73 16.720663431395604 31.18007062660217 33.624792774863614 18.474473167138612 74-75 16.208058647925867 32.415728590086445 33.22792928664718 18.148283475340506 76-77 16.67065025815616 31.29509474146491 33.80164823526897 18.232606765109956 78-79 16.230872055520585 31.4797976265417 33.916400488058876 18.37292982987884 80-81 16.64257514998912 31.55172681774379 33.29777891759147 18.50791911467562 82-83 16.391980538154233 31.575950941225848 33.175296513345046 18.856772007274873 84-85 16.252118084163726 30.505852907799213 33.2937568983475 19.948272109689555 86-87 16.761520958944836 30.724824103418058 33.356714964956694 19.15693997268041 88-89 17.719718287961943 29.6942289454455 33.46418743489685 19.121865331695716 90-91 16.77681323935887 29.524400057535388 34.257074325611406 19.441712377494333 92-93 17.254898149587934 30.168908412377547 33.866039496190325 18.710153941844194 94-95 17.17778771210325 29.39551789150421 33.683453517075165 19.743240879317383 96-97 17.510812202968133 28.763861487175994 32.89553225195105 20.829794057904813 98-99 17.48641420541566 28.895177528823652 32.7687810490799 20.84962721668079 100-101 18.15122196723414 28.274011359816253 33.792830063289294 19.781936609660313 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5254.0 1 3072.5 2 1044.5 3 1107.0 4 867.0 5 846.5 6 1415.0 7 2706.5 8 3806.0 9 3779.0 10 2740.0 11 1732.0 12 1452.5 13 1351.5 14 1273.0 15 1181.0 16 1043.0 17 971.5 18 939.5 19 966.5 20 1011.5 21 1079.5 22 1203.0 23 1310.0 24 1407.0 25 1828.0 26 2837.0 27 3882.0 28 4094.5 29 4379.0 30 5475.0 31 6600.0 32 7822.0 33 9419.5 34 11145.0 35 11951.5 36 12529.5 37 13568.0 38 12630.0 39 10597.5 40 9405.0 41 8436.0 42 7393.5 43 7053.5 44 7101.0 45 6487.0 46 5823.0 47 5788.5 48 10100.5 49 9892.5 50 4741.5 51 3076.5 52 1948.5 53 1489.0 54 1271.0 55 1148.0 56 1009.0 57 874.0 58 817.0 59 721.0 60 626.0 61 572.5 62 524.5 63 429.0 64 310.0 65 235.0 66 192.0 67 183.5 68 156.0 69 127.5 70 119.0 71 92.5 72 76.5 73 81.5 74 62.5 75 39.5 76 30.5 77 26.0 78 19.5 79 13.0 80 11.0 81 8.0 82 4.5 83 1.0 84 2.0 85 3.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2803821562317258 2 0.10533694267269257 3 0.11811679233518836 4 0.12005313319314226 5 0.12470035125223163 6 0.05770295756702643 7 0.06428651648406973 8 0.0689337345431591 9 0.06622285734202363 10-11 0.061382005197138864 12-13 0.06622285734202363 14-15 0.06235017562611581 16-17 0.06370561422668355 18-19 0.06835283228577292 20-21 0.07087007540111301 22-23 0.08132631603406411 24-25 0.0896525817232659 26-27 0.09120165440962903 28-29 0.0834562909778134 30-31 0.08035814560508715 32-33 0.07125734357270379 34-35 0.23681448692776286 36-37 0.3580294246356775 38-39 0.3361487729407983 40-41 0.320658046077167 42-43 0.338472381970343 44-45 0.3493158907748849 46-47 0.35667398603510975 48-49 0.36635569032487925 50-51 0.3595784973220406 52-53 0.35996576549363135 54-55 0.3477668180885218 56-57 0.3262734345652334 58-59 0.3175599007044408 60-61 0.2759285722584318 62-63 0.3363424070265937 64-65 0.36461298355272076 66-67 0.3684856652686285 68-69 0.3820400512743059 70-71 0.3822336853601013 72-73 0.3680983970970378 74-75 0.36984110386919633 76-77 0.3537694747481789 78-79 0.3380851137987522 80-81 0.3357615047692076 82-83 0.3466050135737494 84-85 0.3528013043192019 86-87 0.34641137948795403 88-89 0.3620957404373807 90-91 0.38184641718851053 92-93 0.37913553998737504 94-95 0.3771991991294211 96-97 0.3820400512743059 98-99 0.37371378558510415 100-101 0.35183313389022497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 258219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.80910002749604 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3844307064402 18.16558812480879 2 10.698637536762499 5.522444126884544 3 4.773122861773003 3.6957001614908274 4 2.768441270031811 2.8580391063399673 5 1.8546305743952944 2.3933173004310295 6 1.2889382390012605 1.9959801563788875 7 1.0728647740231678 1.9382771988118612 8 0.7877678410659623 1.6265263206812823 9 0.6197107016385571 1.4394757938029348 >10 4.90966928755777 25.28706253219167 >50 0.5251785607106416 9.466770454536654 >100 0.29109897365104137 13.413033123046716 >500 0.012004081387671808 2.220982964073132 >1k 0.010503571214212831 5.656051646083363 >5k 0.003001020346917952 4.3207509904383485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5966 2.3104419117106025 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5191 2.0103090787277464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4200 1.6265263206812823 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 1.2260910312564142 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2091 0.809777746796324 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1605 0.6215654154032042 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1420 0.5499208036589096 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1078 0.4174750889748624 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1045 0.4046952393123666 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 969 0.37526285827146727 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 947 0.36674295849647004 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 700 0.27108772011354704 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 693 0.26837684291241154 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 642 0.24862616616128172 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 621 0.2404935345578753 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 586 0.22693914855219793 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 577 0.22345373500788093 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 498 0.19285954945220918 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 493 0.19092320859425527 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 490 0.18976140407948291 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 418 0.16187809572494666 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 415 0.1607162912101743 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 413 0.15994175486699275 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 406 0.15723087766585728 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 389 0.15064731874881399 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 371 0.14367649166017993 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 358 0.13864200542949978 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 355 0.1374802009147274 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 350 0.13554386005677352 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 349 0.13515659188518275 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 336 0.13012210565450258 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 311 0.12044040136473302 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 296 0.11463137879087132 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 295 0.11424411061928053 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 295 0.11424411061928053 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 294 0.11385684244768976 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 291 0.11269503793291741 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 291 0.11269503793291741 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 288 0.11153323341814507 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 288 0.11153323341814507 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 286 0.1107586970749635 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 281 0.1088223562170096 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 277 0.10727328353064647 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 272 0.10533694267269257 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 269 0.1041751381579202 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 267 0.10340060181473865 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 261 0.10107699278519397 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9363408579539074E-4 0.0 20-21 0.0 0.0 0.0 0.0011618045147723443 0.0 22-23 0.0 0.0 0.0 0.0027108772011354702 0.0 24-25 0.0 0.0 0.0 0.009875338375564927 0.0 26-27 0.0 0.0 0.0 0.017814335893175947 0.0 28-29 0.0 0.0 0.0 0.037952280815896584 0.0 30-31 0.0 0.0 0.0 0.07784090248974708 0.0 32-33 0.0 0.0 0.0 0.14464466208915688 0.0 34-35 0.0 0.0 0.0 0.23758902327094444 0.0 36-37 0.0 0.0 0.0 0.380103710416352 0.0 38-39 0.0 0.0 0.0 0.5468226582861835 0.0 40-41 0.0 0.0 0.0 0.7187697264724904 0.0 42-43 0.0 0.0 0.0 0.8787114813394832 0.0 44-45 0.0 0.0 0.0 1.0893853666848683 0.0 46-47 0.0 0.0 0.0 1.3349133874734238 0.0 48-49 0.0 0.0 0.0 1.5726960448301635 0.0 50-51 0.0 0.0 0.0 1.8193858701334913 0.0 52-53 0.0 0.0 0.0 2.036256046224329 0.0 54-55 0.0 0.0 0.0 2.2994047688202652 0.0 56-57 0.0 0.0 0.0 2.600118504060507 0.0 58-59 0.0 0.0 0.0 2.9345245702291463 0.0 60-61 0.0 0.0 0.0 3.3150155488170894 0.0 62-63 0.0 0.0 0.0 3.6701404621658362 0.0 64-65 0.0 0.0 0.0 4.05895770644298 0.0 66-67 0.0 0.0 0.0 4.48495269519284 0.0 68-69 0.0 0.0 0.0 4.955677157761436 0.0 70-71 0.0 0.0 0.0 5.50830883862148 0.0 72-73 0.0 0.0 0.0 6.070234955599704 0.0 74-75 0.0 0.0 0.0 6.658495308246101 0.0 76-77 0.0 0.0 0.0 7.284901575794191 0.0 78-79 0.0 0.0 0.0 7.991278720775775 0.0 80-81 0.0 0.0 0.0 8.733671805715304 0.0 82-83 0.0 0.0 0.0 9.44973065498666 0.0 84-85 0.0 0.0 0.0 10.229495118484696 0.0 86-87 0.0 0.0 0.0 11.068124344064534 0.0 88-89 0.0 0.0 0.0 11.89378008589608 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGAGT 65 2.626719E-6 43.735733 6 GTGGTAC 45 9.647955E-4 42.189445 1 CATATAG 45 9.712649E-4 42.132214 3 AATAGAC 115 2.5484042E-9 37.080296 7 ACACTGA 85 1.6556509E-5 33.44497 6 TAGGCAT 115 1.0506119E-7 32.973038 5 GGCATTC 115 1.0541953E-7 32.960262 7 CGGTCAT 30 0.0039264653 31.703611 34-35 GTCATAC 30 0.003935819 31.688206 36-37 AGCTAGG 30 0.0039979974 31.586918 10-11 ATACCTT 90 2.4475934E-5 31.586918 6 CTTTAGG 125 2.183424E-7 30.335194 2 TAATAGA 145 2.4439942E-8 29.408508 6 GCATTCC 145 2.4439942E-8 29.408508 8 CCTTTAG 130 3.0420415E-7 29.20808 1 CTTAGGG 65 0.005896957 29.168455 2 GGGTATG 65 0.0059081377 29.157154 7 ATACTCA 65 0.0059081377 29.157154 6 ACTCAAT 115 3.925634E-6 28.840229 8 ATGGGGC 100 5.010797E-5 28.439243 5 >>END_MODULE