##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 258219 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.048466611674584 33.0 31.0 34.0 30.0 34.0 2 32.2493852117776 34.0 31.0 34.0 30.0 34.0 3 32.28066098931527 34.0 31.0 34.0 30.0 34.0 4 35.90811288092666 37.0 35.0 37.0 35.0 37.0 5 35.86195438755475 37.0 35.0 37.0 35.0 37.0 6 35.92926159577723 37.0 35.0 37.0 35.0 37.0 7 35.87629105526704 37.0 35.0 37.0 35.0 37.0 8 35.880272172070995 37.0 35.0 37.0 35.0 37.0 9 37.49325959747346 39.0 37.0 39.0 34.0 39.0 10-11 37.4371425030691 39.0 37.0 39.0 34.0 39.0 12-13 37.370896409636785 39.0 37.0 39.0 34.0 39.0 14-15 38.473745154307004 40.0 38.0 41.0 34.0 41.0 16-17 38.42006784938366 40.0 38.0 41.0 33.5 41.0 18-19 38.56194741672766 40.0 38.0 41.0 34.0 41.0 20-21 38.60347418276734 40.0 38.0 41.0 34.0 41.0 22-23 38.500640928823984 40.0 38.0 41.0 34.0 41.0 24-25 38.38377307634218 40.0 38.0 41.0 34.0 41.0 26-27 38.11137251712694 40.0 37.0 41.0 33.5 41.0 28-29 37.876571824691446 40.0 36.5 41.0 33.0 41.0 30-31 37.54487082670137 39.5 36.0 41.0 33.0 41.0 32-33 37.13504234777456 39.0 35.0 41.0 33.0 41.0 34-35 36.876318164039056 39.0 35.0 41.0 32.0 41.0 36-37 36.646623602445985 39.0 35.0 41.0 31.0 41.0 38-39 36.42476928498677 39.0 35.0 41.0 31.0 41.0 40-41 36.18490312486688 39.0 35.0 41.0 30.5 41.0 42-43 35.89735457111986 39.0 35.0 41.0 30.0 41.0 44-45 35.500803581456054 38.5 35.0 41.0 28.0 41.0 46-47 34.9615733156739 38.0 35.0 41.0 24.5 41.0 48-49 34.45390540587641 38.0 34.5 40.0 22.5 41.0 50-51 34.1866981128422 38.0 34.0 40.0 20.5 41.0 52-53 33.96653228461113 38.0 34.0 40.0 20.0 41.0 54-55 33.700577804112015 38.0 34.0 40.0 18.0 41.0 56-57 33.4538550610141 38.0 33.0 40.0 13.5 41.0 58-59 33.153615729284056 38.0 33.0 40.0 7.5 41.0 60-61 32.79248041391222 37.0 33.0 40.0 2.0 41.0 62-63 32.36948675349219 36.5 32.5 40.0 2.0 41.0 64-65 31.894990298932303 36.0 32.0 39.0 2.0 41.0 66-67 31.48377927263292 36.0 31.0 39.0 2.0 40.0 68-69 31.048360112927398 35.0 31.0 38.5 2.0 40.0 70-71 30.67879590580089 35.0 31.0 38.0 2.0 40.0 72-73 30.155759258613813 35.0 30.0 37.0 2.0 39.5 74-75 29.594892320084888 35.0 29.5 37.0 2.0 39.0 76-77 27.608072992304983 32.5 26.5 35.0 2.0 37.0 78-79 28.51659056847095 34.0 28.5 36.0 2.0 37.5 80-81 28.49478543406953 34.0 29.0 35.0 2.0 37.0 82-83 28.14232298940047 34.0 28.5 35.0 2.0 37.0 84-85 27.74258091000275 34.0 27.5 35.0 2.0 36.0 86-87 27.20113159759739 34.0 26.0 35.0 2.0 36.0 88-89 26.61939284096058 33.0 25.0 35.0 2.0 35.5 90-91 26.14950487764262 33.0 24.5 35.0 2.0 35.0 92-93 25.79727285753566 33.0 23.0 35.0 2.0 35.0 94-95 25.37678288584496 33.0 19.5 35.0 2.0 35.0 96-97 24.78848767906312 32.5 9.5 35.0 2.0 35.0 98-99 24.081570294982164 32.0 2.0 34.5 2.0 35.0 100-101 21.03699185575035 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30303984707512654 1101 2 0.2771287216761493 1101 3 0.3161911506025419 1101 4 0.12785481416059952 1101 5 0.1994071045095609 1101 6 0.30521806030940013 1101 7 0.1956393558300391 1101 8 0.24868113589275964 1101 9 0.17370291033390117 1101 10-11 0.3001440351586311 1101 12-13 0.31586425429055254 1101 14-15 0.45907282241339686 1101 16-17 0.1666883704008626 1101 18-19 -0.012860747401177264 1101 20-21 0.09764787150880494 1101 22-23 0.42493114235804796 1101 24-25 0.3006538756592434 1101 26-27 0.12656809104202438 1101 28-29 0.010614634866186634 1101 30-31 0.08206901748239659 1101 32-33 0.15522289894538943 1101 34-35 0.48166036401711665 1101 36-37 0.37630084913038786 1101 38-39 0.3750029721851931 1101 40-41 0.3587199725940238 1101 42-43 0.5850669998031961 1101 44-45 0.6058419111218427 1101 46-47 0.5496769448652188 1101 48-49 0.6406277659786426 1101 50-51 0.5141179616992346 1101 52-53 0.7066810526366538 1101 54-55 0.02680116165537072 1101 56-57 0.15748585814638716 1101 58-59 0.02072204658664134 1101 60-61 -0.22445101149206437 1101 62-63 -0.34415695885208564 1101 64-65 -0.4612795170380011 1101 66-67 -0.03465528331273049 1101 68-69 -0.3307391703364324 1101 70-71 -0.3605351739314955 1101 72-73 -0.001860283222743675 1101 74-75 -0.5761542577874899 1101 76-77 -0.6304449318067888 1101 78-79 -0.4655126236583733 1101 80-81 -0.4809827944547287 1101 82-83 -0.7889625473474986 1101 84-85 -0.1966909088476676 1101 86-87 -0.08925565756994658 1101 88-89 0.40007508968334093 1101 90-91 0.2486604815393001 1101 92-93 0.26750610721257573 1101 94-95 0.24485336049018613 1101 96-97 0.19146887006733238 1101 98-99 0.4954633089502565 1101 100-101 0.5015946184591442 1102 1 0.45467886761224463 1102 2 0.47177722088625984 1102 3 0.43240363085531186 1102 4 0.17982953769930532 1102 5 0.18127914242425192 1102 6 0.3988989449855467 1102 7 0.354012183634147 1102 8 0.2960152591155065 1102 9 0.2882961172849292 1102 10-11 0.4420772895030254 1102 12-13 0.4191817898355694 1102 14-15 0.6796889361574685 1102 16-17 0.3809952424387717 1102 18-19 0.6241850661849213 1102 20-21 0.5272489773539917 1102 22-23 0.20033596068348203 1102 24-25 0.28691967344755653 1102 26-27 0.5880412348018993 1102 28-29 0.3572674784680743 1102 30-31 0.5881807868346911 1102 32-33 0.7385364850433334 1102 34-35 0.8949008853441356 1102 36-37 0.8199224921003747 1102 38-39 0.7293967320588166 1102 40-41 1.1594308730679543 1102 42-43 1.0299129713671746 1102 44-45 1.1666140280254709 1102 46-47 1.1772737063186085 1102 48-49 1.5483884294857546 1102 50-51 1.5302020849219815 1102 52-53 1.5643627272022087 1102 54-55 1.6094433417501506 1102 56-57 1.6494882278146363 1102 58-59 1.7990593293670472 1102 60-61 1.4875158131524842 1102 62-63 1.855912156471767 1102 64-65 2.17218217332535 1102 66-67 1.8789209410158634 1102 68-69 1.84007441576151 1102 70-71 2.054818301581932 1102 72-73 2.1661195745971646 1102 74-75 1.5406111450561149 1102 76-77 1.6102738675613004 1102 78-79 1.716665496404815 1102 80-81 1.9045721028596496 1102 82-83 1.9462171524945227 1102 84-85 1.906922835228162 1102 86-87 2.2213486078328977 1102 88-89 2.358773746871332 1102 90-91 2.820303451523504 1102 92-93 2.4075732478128913 1102 94-95 2.328305177235844 1102 96-97 2.4479657105096706 1102 98-99 2.2156548571335115 1102 100-101 2.5777597053469776 1103 1 0.04943371852505152 1103 2 0.08619075456105207 1103 3 0.3263742597206303 1103 4 0.17075466468376987 1103 5 0.25445194755888423 1103 6 0.2512569242854852 1103 7 0.045041448505671156 1103 8 0.07530669643087151 1103 9 0.08773504785258979 1103 10-11 0.0829822264889799 1103 12-13 0.1181064067569153 1103 14-15 0.19292932465554458 1103 16-17 0.3801132582932354 1103 18-19 0.5405772197139171 1103 20-21 0.3169116981156819 1103 22-23 0.4167939973655166 1103 24-25 0.1754390027145476 1103 26-27 0.468813980429168 1103 28-29 0.43267180975407626 1103 30-31 0.28168598310272586 1103 32-33 0.007371628392327523 1103 34-35 0.17120632814268077 1103 36-37 0.37564688799436396 1103 38-39 0.0694069333212255 1103 40-41 -0.03881365969296979 1103 42-43 -0.23460788808917954 1103 44-45 -0.089458911000726 1103 46-47 -0.5254445050600438 1103 48-49 -0.4266928618240513 1103 50-51 -0.2846768813501015 1103 52-53 -0.39367395483719037 1103 54-55 -0.299712291259425 1103 56-57 -0.3422338727953189 1103 58-59 -0.5600888652220348 1103 60-61 -0.32568419534856474 1103 62-63 -1.054741786953727 1103 64-65 -0.6032731642726823 1103 66-67 -0.18851365401527076 1103 68-69 -0.03458819873703334 1103 70-71 -0.30419175091206085 1103 72-73 -0.2887717182003193 1103 74-75 -0.28114304702515724 1103 76-77 -0.23900248038675898 1103 78-79 -0.40513332619947917 1103 80-81 -0.6476774880272238 1103 82-83 -0.3636355667794824 1103 84-85 -0.6172626577265952 1103 86-87 -0.3224955678838448 1103 88-89 -0.36019210015223635 1103 90-91 -0.3588880984308034 1103 92-93 0.03299564383440057 1103 94-95 -0.21519895640069464 1103 96-97 -0.5055229087069542 1103 98-99 -0.5931671095848685 1103 100-101 0.1447373688328355 1104 1 0.05374331027858403 1104 2 0.017334349381776804 1104 3 -0.0931703212589241 1104 4 0.09885048515626949 1104 5 0.3018962819987365 1104 6 0.06322671831805593 1104 7 0.16425407444475582 1104 8 0.12849715320877664 1104 9 0.006008105139095221 1104 10-11 0.23804987931448096 1104 12-13 0.12668676511305677 1104 14-15 -0.0991522858117122 1104 16-17 0.07510287256536685 1104 18-19 0.2393037547633199 1104 20-21 -0.07307723671630129 1104 22-23 -0.15269331651641238 1104 24-25 0.3781159968713652 1104 26-27 0.2490248009987397 1104 28-29 0.3970594400609855 1104 30-31 0.10947702614040367 1104 32-33 0.37454108076357073 1104 34-35 -0.1987779476711964 1104 36-37 0.07841123874078448 1104 38-39 0.28655600248823276 1104 40-41 0.5922697561438142 1104 42-43 0.33014275737895105 1104 44-45 0.3354144219443498 1104 46-47 0.10002867646694824 1104 48-49 0.02715374000462134 1104 50-51 -0.1935930772618093 1104 52-53 0.2451550786106793 1104 54-55 0.049257871612084614 1104 56-57 -0.2729795098189811 1104 58-59 -0.3956740573094706 1104 60-61 -0.47888824092929383 1104 62-63 -0.24780955625467982 1104 64-65 0.01542502841654425 1104 66-67 -0.3623338547413013 1104 68-69 -0.11915908375634743 1104 70-71 -0.3900535721998928 1104 72-73 -0.4604371230495836 1104 74-75 -0.25196594609917256 1104 76-77 -0.45890813526998997 1104 78-79 -0.7058535327492841 1104 80-81 -0.841832361554296 1104 82-83 -0.44120821834316715 1104 84-85 -0.7676324672892285 1104 86-87 -0.7351972160115032 1104 88-89 -0.6031987198404671 1104 90-91 -0.6844564015775809 1104 92-93 -0.5500804728740043 1104 94-95 -0.39897780834098384 1104 96-97 0.04228055837902289 1104 98-99 -0.030862448625498473 1104 100-101 -0.03092702656250168 1105 1 0.05720651374178942 1105 2 -0.0728712783238521 1105 3 0.024524483935877583 1105 4 0.16118814749393806 1105 5 0.1994071045095609 1105 6 0.1635513936427344 1105 7 -0.11686064416996089 1105 8 -0.06798553077391034 1105 9 0.05703624366723403 1105 10-11 -0.15610596484136607 1105 12-13 -0.11538574570945315 1105 14-15 0.0049061557467311445 1105 16-17 0.056271703734196876 1105 18-19 -0.023277414067848667 1105 20-21 -0.23985212849119364 1105 22-23 0.07909780902471653 1105 24-25 0.09023720899257626 1105 26-27 0.2474014243753615 1105 28-29 0.002281301532846669 1105 30-31 0.07998568414906515 1105 32-33 -0.18644376772127913 1105 34-35 -0.12042296931621621 1105 36-37 0.3992175157970621 1105 38-39 0.8479196388518631 1105 40-41 0.7712199725940323 1105 42-43 0.5225669998031961 1105 44-45 0.5829252444551756 1105 46-47 0.7705102781985502 1105 48-49 0.9302110993119754 1105 50-51 0.9599512950325604 1105 52-53 0.7191810526366567 1105 54-55 1.3184678283220421 1105 56-57 1.10748585814639 1105 58-59 0.9144720465866385 1105 60-61 1.4109656551746 1105 62-63 1.3058430411479165 1105 64-65 1.372053816295331 1105 66-67 1.436178050020601 1105 68-69 1.2400941629969005 1105 70-71 1.4457148260685102 1105 72-73 1.2835563834439263 1105 74-75 0.9905124088791801 1105 76-77 0.9362217348598776 1105 78-79 0.8178207096749581 1105 80-81 0.7294338722119384 1105 82-83 0.8318707859858385 1105 84-85 0.9012257578189953 1105 86-87 0.9919943424300541 1105 88-89 1.3167417563500088 1105 90-91 1.571577148205968 1105 92-93 1.4404227738792414 1105 94-95 1.4302700271568547 1105 96-97 1.312302203400666 1105 98-99 1.212129975616925 1105 100-101 1.45576128512581 1106 1 -0.09645202284357524 1106 2 -0.0209403840149065 1106 3 -0.06439828029176198 1106 4 -0.10873055169305701 1106 5 -0.0016091556530426487 1106 6 -0.16795267139792003 1106 7 0.11637106314711332 1106 8 -0.1855668315869181 1106 9 -0.1481466831620324 1106 10-11 -0.004479948581199267 1106 12-13 0.19106750632307268 1106 14-15 0.15744680615323148 1106 16-17 0.009930240319562245 1106 18-19 0.10909047211101353 1106 20-21 0.07173323736246573 1106 22-23 0.16390471959381614 1106 24-25 0.02926159923647731 1106 26-27 0.08988109917211062 1106 28-29 -0.03618414562161831 1106 30-31 0.1597621069132913 1106 32-33 0.4589830615470092 1106 34-35 0.43608109572443965 1106 36-37 0.13798784100031014 1106 38-39 0.41072451690064327 1106 40-41 0.3066874522688252 1106 42-43 0.6172824469576668 1106 44-45 0.6782504477072067 1106 46-47 1.1245245058408315 1106 48-49 0.49418467654774645 1106 50-51 0.633985847878094 1106 52-53 1.1805631664577874 1106 54-55 1.095398722630982 1106 56-57 1.1031159394471999 1106 58-59 1.3850513148793269 1106 60-61 1.2038010210282621 1106 62-63 1.418698732204824 1106 64-65 1.2321656049132166 1106 66-67 0.9225093508336144 1106 68-69 0.6682445695009633 1106 70-71 1.203336777288019 1106 72-73 0.8854872777528691 1106 74-75 0.7168335470905625 1106 76-77 1.0494331169737023 1106 78-79 1.497952823496096 1106 80-81 1.29843793725259 1106 82-83 1.278567940457382 1106 84-85 1.132272505786478 1106 86-87 0.7703988139747686 1106 88-89 1.2794958213906575 1106 90-91 1.6600425953604407 1106 92-93 1.6682174893263984 1106 94-95 1.1113167751243331 1106 96-97 1.0958387887665175 1106 98-99 1.482810869925867 1106 100-101 1.2848263257762156 1107 1 -0.08412681959154256 1107 2 0.07896205500948383 1107 3 0.24002448393587628 1107 4 0.15452148082727035 1107 5 0.14440710450956118 1107 6 0.24788472697606778 1107 7 0.23630602249670574 1107 8 0.2391811358927569 1107 9 0.03786957700057059 1107 10-11 0.22856070182530175 1107 12-13 0.3038642542905521 1107 14-15 0.35907282241339544 1107 16-17 0.12693837040086464 1107 18-19 0.41380591926548504 1107 20-21 0.4284812048421429 1107 22-23 0.3088478090247122 1107 24-25 0.20423720899258058 1107 26-27 0.29040142437536076 1107 28-29 0.2058646348661881 1107 30-31 0.11215235081572672 1107 32-33 0.021389565612054184 1107 34-35 -0.1830896359828813 1107 36-37 -0.27653248420294574 1107 38-39 -0.3444136944814673 1107 40-41 -0.2992800274059704 1107 42-43 -0.5594330001968117 1107 44-45 -0.5927414222114962 1107 46-47 -0.9829063884681162 1107 48-49 -0.8628722340213528 1107 50-51 -0.8946320383007631 1107 52-53 -0.7914856140300088 1107 54-55 -1.1766988383446275 1107 56-57 -0.8170141418536119 1107 58-59 -0.8851112867466924 1107 60-61 -1.0354510114920643 1107 62-63 -1.1830736255187517 1107 64-65 -0.8773628503713375 1107 66-67 -0.7295719499793947 1107 68-69 -0.7310725036697683 1107 70-71 -0.912451840598159 1107 72-73 -0.8026102832227444 1107 74-75 -0.8956542577874878 1107 76-77 -0.6627782651401191 1107 78-79 -0.8434292903250444 1107 80-81 -0.8053994611213966 1107 82-83 -0.47154588068083214 1107 84-85 -0.3151909088476721 1107 86-87 -0.5127556575699437 1107 88-89 -0.28100824364999255 1107 90-91 -0.4286728517940297 1107 92-93 -0.6973272261207555 1107 94-95 -0.5888133061764798 1107 96-97 -1.1866977965993328 1107 98-99 -0.6826200243830769 1107 100-101 -1.342572048207522 1108 1 0.042647126768606824 1108 2 0.037138300220213694 1108 3 0.045357817269213285 1108 4 -0.024252465532882184 1108 5 0.005633158149407791 1108 6 0.15234449709100772 1108 7 0.165945869239998 1108 8 0.1341696416398861 1108 9 0.30162436627259837 1108 10-11 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-0.9204776115453512 2316 78-79 -0.9993770027433406 2316 80-81 -1.2567589382455786 2316 82-83 -1.22446091336057 2316 84-85 -1.5327203206123734 2316 86-87 -1.6734876837137342 2316 88-89 -2.435831773061757 2316 90-91 -2.247417949833249 2316 92-93 -1.9193402980161807 2316 94-95 -1.6141335676143882 2316 96-97 -1.7424363586908385 2316 98-99 -1.763482769481115 2316 100-101 -1.052572048207523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 28.0 6 86.0 7 147.0 8 223.0 9 351.0 10 589.0 11 866.0 12 1194.0 13 1576.0 14 2001.0 15 2646.0 16 3524.0 17 4814.0 18 4278.0 19 2647.0 20 2353.0 21 2381.0 22 2626.0 23 2678.0 24 3007.0 25 3274.0 26 3597.0 27 3989.0 28 4674.0 29 5440.0 30 6373.0 31 7645.0 32 9214.0 33 11216.0 34 15858.0 35 25978.0 36 28689.0 37 48126.0 38 42969.0 39 3154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.872247893449305 25.558964004193683 17.040733118471636 17.528054983885372 2 14.584132073937239 26.02287205821415 39.65897164809716 19.73402421975145 3 17.11609137979777 31.525952776519155 34.33829423861141 17.019661605071665 4 10.635158528226041 23.0045039288356 39.971884330742505 26.38845321219585 5 9.134107095140171 42.61847501539391 35.70651268884164 12.540905200624275 6 21.880264426707562 43.74116544483558 18.91572657318013 15.462843555276724 7 20.8787889349738 40.9175932057672 22.406561871899434 15.797055987359569 8 22.78531014371522 36.11662968255628 21.503839763921324 19.59422040980718 9 24.288685185830634 20.251801765168327 23.94595285397279 31.51356019502825 10-11 21.02672537652148 31.08349889047669 28.18518389429128 19.704591838710552 12-13 23.76413044741092 31.1419763843869 28.342608406042935 16.75128476215925 14-15 21.208571185141007 31.610795696592746 29.61555144182732 17.565081676438922 16-17 15.518833855823653 34.86465919366738 30.899025437759107 18.717481512749863 18-19 14.93189889202576 32.55957152649495 36.43205960831697 16.076469973162315 20-21 16.67034571429678 30.994233576925012 36.0234916872887 16.31192902148951 22-23 16.66414942355133 31.093374228852255 35.497387876182614 16.745088471413798 24-25 16.325483407495188 32.11673037228089 36.213640359539774 15.344145860684147 26-27 16.353718681803837 31.3847101172853 37.06502101918351 15.19655018172735 28-29 15.085063453889916 31.4064805455834 37.87172903620571 15.636726964320982 30-31 15.838300047633986 32.02184967024115 36.802287980357754 15.337562301767104 32-33 15.41385413157049 32.678269221087525 36.393332791157896 15.514543856184094 34-35 15.633768180207463 31.709031479763837 36.48356882967002 16.17363151035868 36-37 16.665698338736604 31.334704485682302 35.79288627569458 16.206710899886513 38-39 15.829303857646062 31.938815227641975 36.372368092361974 15.859512822349986 40-41 14.95767463208594 32.065799437211076 37.408227253624915 15.568298677078069 42-43 15.138793412275012 32.31006070698953 36.272184192945595 16.27896168778986 44-45 15.555163980613168 32.509735047915214 35.41969626263233 16.515404708839288 46-47 16.1359156375015 32.384139044764325 34.186872383519415 17.293072934214756 48-49 16.185679597550916 32.06483643728773 34.493201507247726 17.25628245791363 50-51 16.049361201150962 32.4526855111359 34.28775574221881 17.210197545494328 52-53 15.676809220080628 33.21115022519644 34.78171629508285 16.330324259640072 54-55 15.745936588709583 32.34560586169105 34.28717483996142 17.621282709637942 56-57 16.40952060073039 33.51554300806679 32.855444409590305 17.219491981612506 58-59 15.944217892564064 32.333019646114344 33.93185629252689 17.790906168794702 60-61 16.215525404168226 32.90836529952695 33.45306536594433 17.423043930360492 62-63 16.613448844085656 32.50308366461348 33.67361757911512 17.209849912185753 64-65 16.23663680177834 32.4949606631593 33.90190865487949 17.36649388018287 66-67 15.8545653108408 32.631797040496636 33.26381869653279 18.249818952129782 68-69 15.417399251794993 32.442354909417624 33.55401250106499 18.58623333772239 70-71 16.035419546973692 32.64341508564436 33.40052436110433 17.92064100627762 72-73 16.427334936623563 31.236469818255046 33.2382589972078 19.097936247913594 74-75 15.859018894814092 32.4902505237802 32.65909944659378 18.99163113481192 76-77 16.268879449029612 31.407563229052858 33.56199872309624 18.76155859882129 78-79 15.60903729005224 31.430684806307823 33.67625929927697 19.284018604362963 80-81 16.06600675403538 31.481395420887935 33.041136103107476 19.411461721969204 82-83 15.94983328105213 31.525371874261772 32.87461418408405 19.650180660602047 84-85 15.539716287337493 30.55216695905414 33.06863553805103 20.83948121555734 86-87 16.07054253950041 30.750430279255113 33.01889352332811 20.16013365791636 88-89 16.81250364268646 29.92772910069357 33.060439453694165 20.199327802925808 90-91 15.995873436510372 29.58439055475468 33.87026581752197 20.549470191212986 92-93 16.212207467304886 30.3269705172741 33.30932270669471 20.151499308726315 94-95 16.19691037452705 29.537330715400493 33.13834380893738 21.12741510113508 96-97 16.484650626018997 29.049953721453498 32.290613781325156 22.174781871202352 98-99 16.295663758282696 29.190338433655157 32.24627157567801 22.26772623238414 100-101 16.541675138207133 28.586366144819774 33.42472915274914 21.447229564223957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4781.0 1 2847.0 2 1003.5 3 1035.0 4 826.0 5 822.0 6 1412.5 7 2686.5 8 3739.5 9 3709.0 10 2643.0 11 1618.0 12 1377.0 13 1298.5 14 1209.0 15 1108.5 16 972.0 17 937.5 18 952.5 19 927.0 20 982.0 21 1081.5 22 1146.5 23 1218.0 24 1336.5 25 1708.0 26 2635.0 27 3815.5 28 4177.5 29 4417.0 30 5511.5 31 6642.0 32 7921.0 33 9497.0 34 11138.5 35 12056.0 36 12675.0 37 13600.0 38 12570.5 39 10583.0 40 9403.0 41 8530.0 42 7632.5 43 7301.5 44 7220.0 45 6593.5 46 5942.5 47 6075.0 48 10403.5 49 10004.5 50 4795.0 51 3281.0 52 2517.0 53 1930.0 54 1397.0 55 1143.0 56 978.0 57 787.5 58 680.0 59 611.5 60 506.5 61 464.0 62 429.5 63 323.5 64 233.0 65 160.0 66 103.0 67 103.0 68 91.0 69 65.5 70 50.5 71 45.5 72 46.5 73 40.5 74 31.5 75 25.0 76 19.5 77 11.0 78 5.0 79 5.0 80 5.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2668277702260484 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0019363408579539073 16-17 1.936340857953907E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.681704289769536E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002129974943749298 36-37 0.016265263206812822 38-39 0.006777193002838675 40-41 0.01568436094942665 42-43 0.004453583973293987 44-45 9.681704289769536E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0013554386005677351 62-63 0.0013554386005677351 64-65 1.936340857953907E-4 66-67 0.0 68-69 3.872681715907814E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21861288286299613 78-79 0.0 80-81 0.0011618045147723445 82-83 0.0 84-85 0.0 86-87 0.09565523838292303 88-89 0.3307270185385274 90-91 0.3338251639112536 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.809022573861723E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 258219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.734818119503213 #Duplication Level Percentage of deduplicated Percentage of total 1 68.88680131517144 17.0390250136512 2 11.049005793017066 5.465902973832289 3 5.224675121340222 3.876941665795313 4 2.902771254109911 2.871980760517235 5 1.9320494754971034 2.389444618715122 6 1.4372945044621888 2.1330730891220244 7 1.077188038202599 1.8650835143812037 8 0.8720839204634413 1.725666972608522 9 0.6106153123532174 1.359311282283643 >10 5.115077501174261 25.510516267199545 >50 0.5620792234225771 9.707651257266118 >100 0.29904493502426804 13.252316831836541 >500 0.017222483168936903 2.8464210611922436 >1k 0.010959762016596212 5.769908488531053 >5k 0.0031313605761703464 4.186756203067938 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5740 2.2229193049310854 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5071 1.9638368981368528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4263 1.6509242154915016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3388 1.3120645653495677 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2022 0.7830562429565601 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1633 0.6324089242077461 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1393 0.5394645630259586 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.45465283344757745 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1026 0.39733714405214177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 970 0.375650126443058 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.322594386935121 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 749 0.29006386052149535 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 736 0.28502937429081515 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 643 0.2490134343328725 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 621 0.2404935345578753 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.22926275758174264 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.21880651694879152 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 555 0.21493383523288373 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 554 0.21454656706129294 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 532 0.20602666728629573 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 427 0.1653635092692637 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 425 0.16458897292608213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 424 0.16420170475449136 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 413 0.15994175486699275 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 405 0.15684360949426648 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 384 0.1487109778908601 TruSeq Adapter, Index 10 (95% over 21bp) GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 379 0.14677463703290616 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.1374802009147274 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 354 0.13709293274313664 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 346 0.13399478737041037 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 345 0.1336075191988196 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 335 0.12973483748291179 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 327 0.12663669211018555 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 325 0.125862155767004 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 324 0.1254748875954132 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 314 0.12160220587950539 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 314 0.12160220587950539 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 314 0.12160220587950539 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 296 0.11463137879087132 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 295 0.11424411061928053 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 294 0.11385684244768976 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 293 0.11346957427609897 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 281 0.1088223562170096 TruSeq Adapter, Index 10 (96% over 28bp) GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 279 0.10804781987382803 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 277 0.10727328353064647 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 270 0.104562406329511 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.10262606547155707 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 260 0.10068972461360318 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 259 0.10030245644201241 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9363408579539074E-4 0.0 20-21 0.0 0.0 0.0 0.0011618045147723443 0.0 22-23 0.0 0.0 0.0 0.0027108772011354702 0.0 24-25 0.0 0.0 0.0 0.009875338375564927 0.0 26-27 0.0 0.0 0.0 0.017814335893175947 0.0 28-29 0.0 0.0 0.0 0.037952280815896584 0.0 30-31 0.0 0.0 0.0 0.07842180474713326 0.0 32-33 0.0 0.0 0.0 0.14561283251813384 0.0 34-35 0.0 0.0 0.0 0.23913809595730756 0.0 36-37 0.0 0.0 0.0 0.38145914901691974 0.0 38-39 0.0 0.0 0.0 0.547597194629365 0.0 40-41 0.0 0.0 0.0 0.7201251650730581 0.0 42-43 0.0 0.0 0.0 0.8810350903690278 0.0 44-45 0.0 0.0 0.0 1.093645316572367 0.0 46-47 0.0 0.0 0.0 1.3438205554200118 0.0 48-49 0.0 0.0 0.0 1.5866376990074316 0.0 50-51 0.0 0.0 0.0 1.8344893288255317 0.0 52-53 0.0 0.0 0.0 2.0519404071737557 0.0 54-55 0.0 0.0 0.0 2.317606372885032 0.0 56-57 0.0 0.0 0.0 2.6179328399536828 0.0 58-59 0.0 0.0 0.0 2.954081612894481 0.0 60-61 0.0 0.0 0.0 3.3353471278256053 0.0 62-63 0.0 0.0 0.0 3.6924083820323057 0.0 64-65 0.0 0.0 0.0 4.083742869424791 0.0 66-67 0.0 0.0 0.0 4.509350590003059 0.0 68-69 0.0 0.0 0.0 4.978719613971086 0.0 70-71 0.0 0.0 0.0 5.527865881286814 0.0 72-73 0.0 0.0 0.0 6.09308377772356 0.0 74-75 0.0 0.0 0.0 6.6825059348847295 0.0 76-77 0.0 0.0 0.0 7.3087185683470235 0.0 78-79 0.0 0.0 0.0 8.01509571332861 0.0 80-81 0.0 0.0 0.0 8.755552457410182 0.0 82-83 0.0 0.0 0.0 9.47238584302472 0.0 84-85 0.0 0.0 0.0 10.24866489297844 0.0 86-87 0.0 0.0 0.0 11.0863259481293 0.0 88-89 0.0 0.0 0.0 11.909077178673916 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 35 2.7688456E-4 54.345257 1 TCCTGAG 65 1.331917E-4 36.5077 2 GTAAGGT 65 1.331917E-4 36.5077 4 GGTACTC 55 0.0025865987 34.516373 8 GACAATG 165 1.8189894E-12 34.516373 7 GGACAAT 165 1.8189894E-12 34.516373 6 TGGCCTA 70 2.0591024E-4 33.90001 5 TAAGGTG 70 2.0591024E-4 33.90001 5 TAGGACA 180 5.456968E-12 31.640005 4 CACAGTA 65 0.0058598593 29.20616 7 GTGGTAC 65 0.0058598593 29.20616 6 TGGTACT 65 0.0058598593 29.20616 7 GGATTGA 135 4.2348256E-7 28.12445 6 CCGGAGT 35 0.008421891 27.120007 30-31 CCTGAGA 70 0.00841423 27.120007 3 ACTCTTA 45 8.984922E-4 26.366673 64-65 GCTTAGC 90 8.9727074E-4 26.366673 3 TGGGACC 90 8.9727074E-4 26.366673 6 CAATGGG 230 1.3460522E-10 24.761745 9 GGGGTGA 160 1.863873E-6 23.730005 9 >>END_MODULE