FastQCFastQC Report
Fri 27 May 2016
SRR939239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438943
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99400.4075536000636341No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56000.2296076620076812No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54400.223047443093176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42420.1739278039708185No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42020.1722877492421922No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39600.16236541813400313No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31510.12919531124753633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.12132304855013011No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25160.10315944243059391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89550.027.9794081
TACATGG90550.026.9140342
ACATGGG94100.025.1428833
CATGGGG60950.021.2361224
AGAGTAC105400.019.6423110-11
GAGTACT57700.019.54187812-13
AGTACTT60900.019.40968112-13
CATGGGA40900.018.6633174
GTACTTT62650.017.81283614-15
ACTATAC5600.017.7801483
TACTTTT66350.017.3908714-15
ACGTAAC2202.9438885E-517.2558961
GTATAGG5050.016.9141941
ATGGGGA39950.016.6145525
TACTATA7150.016.5782262
GTACCGT2304.3672022E-516.4802176
GAGTACA69150.016.4698351
GTATCAA186050.016.0942171
GTCTAGG8300.016.0084821
TAAGGTG8000.016.0011485
Produced by FastQC (version 0.11.5)