FastQCFastQC Report
Fri 27 May 2016
SRR939239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438943
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106480.43658256876031953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.2261225457093503No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52310.21447815713610363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.18118504614498984No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40390.16560452622304006No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37420.15342711986298985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.12993333587541817No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29920.12267609370124682No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26060.10684956557000308No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25980.10652155462427783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88250.025.0266441
TACATGG89650.024.1381022
ACATGGG93750.022.2219313
GTAAGGT7300.020.8025054
TAAGGTG7400.020.521395
ACCGGTC1404.8845075E-420.3381658
GTATAGG5350.019.5734881
AGTACTT64350.019.50588612-13
AGAGTAC111500.019.4717410-11
GAGTACT61600.019.02851112-13
CATGGGG63250.018.9822864
TGTACGC1751.0014006E-418.9822862
TCGTCCG2302.0782354E-618.569639
CGTAAAC1559.689243E-418.3699553
CATGGGA35750.018.3185714
TACTTTT68750.017.91237614-15
GTACTTT65850.017.54816614-15
AGGACCG10850.017.4951935
ACCGAGT8700.016.364048
GGACCGA7600.016.234856
Produced by FastQC (version 0.11.5)