##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2438943 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.331190601830386 34.0 31.0 34.0 31.0 34.0 2 32.54325500841963 34.0 31.0 34.0 31.0 34.0 3 32.63873530459711 34.0 31.0 34.0 31.0 34.0 4 36.1481281030348 37.0 35.0 37.0 35.0 37.0 5 36.084733427554475 37.0 35.0 37.0 35.0 37.0 6 36.11645372606084 37.0 36.0 37.0 35.0 37.0 7 36.05582336282562 37.0 35.0 37.0 35.0 37.0 8 36.02695265941024 37.0 35.0 37.0 35.0 37.0 9 37.74138592004815 39.0 38.0 39.0 35.0 39.0 10-11 37.65519591888781 39.0 37.0 39.0 35.0 39.0 12-13 37.58362147044847 39.0 37.0 39.0 35.0 39.0 14-15 38.885150042456914 40.0 38.0 41.0 35.0 41.0 16-17 38.88816692313023 40.0 38.0 41.0 35.0 41.0 18-19 38.9689148126873 40.0 38.0 41.0 35.0 41.0 20-21 38.930685546976704 40.0 38.0 41.0 35.0 41.0 22-23 38.84940176953705 40.0 38.0 41.0 35.0 41.0 24-25 38.77791936916935 40.0 38.0 41.0 34.0 41.0 26-27 38.61618783218796 40.0 38.0 41.0 34.0 41.0 28-29 38.45128668443666 40.0 38.0 41.0 34.0 41.0 30-31 38.27516961240997 40.0 38.0 41.0 34.0 41.0 32-33 38.26010345465228 40.0 38.0 41.0 34.0 41.0 34-35 38.16979773615046 40.0 38.0 41.0 33.0 41.0 36-37 38.031665356672946 40.0 38.0 41.0 33.0 41.0 38-39 37.899281574026126 40.0 37.0 41.0 33.0 41.0 40-41 37.72566271536481 40.0 37.0 41.0 33.0 41.0 42-43 37.53995132317566 40.0 37.0 41.0 32.5 41.0 44-45 37.307368601890246 40.0 37.0 41.0 32.0 41.0 46-47 37.02367624007613 40.0 36.0 41.0 31.0 41.0 48-49 36.71484655442952 39.0 36.0 41.0 31.0 41.0 50-51 36.44764084277492 39.0 35.0 41.0 30.0 41.0 52-53 36.17628722770479 39.0 35.0 40.0 30.0 41.0 54-55 35.90125578990571 38.5 35.0 40.0 29.0 41.0 56-57 35.536346277875296 38.0 34.5 40.0 28.0 41.0 58-59 35.16967842216894 38.0 34.0 40.0 28.0 41.0 60-61 34.763247029553376 37.0 34.0 40.0 27.0 41.0 62-63 34.380319056246904 37.0 34.0 39.0 26.0 41.0 64-65 34.02299069719956 36.0 33.0 39.0 26.0 40.5 66-67 33.56191555112194 35.5 33.0 39.0 26.0 40.0 68-69 33.05463760325682 35.0 32.5 38.0 25.0 40.0 70-71 32.543236352797095 35.0 32.0 37.0 24.0 39.5 72-73 32.0445539317647 35.0 31.5 37.0 22.5 39.0 74-75 31.535761393357696 34.5 31.0 36.0 21.5 39.0 76-77 29.645562647425542 32.5 28.5 34.5 19.0 36.5 78-79 30.621745772656432 34.0 30.5 35.0 20.0 37.0 80-81 30.64936019414968 34.0 31.0 35.0 20.0 37.0 82-83 30.317682701071732 34.0 30.0 35.0 18.0 36.0 84-85 29.897775593771563 34.0 30.0 35.0 15.0 36.0 86-87 29.325201326968283 34.0 29.0 35.0 7.0 35.0 88-89 28.738558465696002 33.5 29.0 35.0 2.0 35.0 90-91 28.247447152311473 33.0 28.0 35.0 2.0 35.0 92-93 27.878263657658255 33.0 27.0 35.0 2.0 35.0 94-95 27.340615176328434 33.0 26.5 35.0 2.0 35.0 96-97 26.575251246134084 32.5 25.0 34.0 2.0 35.0 98-99 25.54243969621266 32.0 22.5 34.0 2.0 35.0 100-101 21.21064473421478 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2879656475377885 1101 2 0.3268758393904463 1101 3 0.2967402221699018 1101 4 0.19521553873063624 1101 5 0.25592155265132277 1101 6 0.24648925982166503 1101 7 0.27500945572688806 1101 8 0.2506049570172948 1101 9 0.2866700540675424 1101 10-11 0.351022372853393 1101 12-13 0.4009851248107523 1101 14-15 0.37471657282188175 1101 16-17 0.3769774543566129 1101 18-19 0.437978787175453 1101 20-21 0.4343180624625802 1101 22-23 0.37432679775437094 1101 24-25 0.41487551682528334 1101 26-27 0.4772538877934238 1101 28-29 0.3922911450369426 1101 30-31 0.44835806223864694 1101 32-33 0.6024414775497604 1101 34-35 0.5752354158320117 1101 36-37 0.514421420144906 1101 38-39 0.4683484870729515 1101 40-41 0.5485942743316201 1101 42-43 0.5758836655450281 1101 44-45 0.720831061312488 1101 46-47 0.7519485905913896 1101 48-49 0.6517576376057193 1101 50-51 0.5960218816625229 1101 52-53 0.5382972970572979 1101 54-55 0.5284154250009578 1101 56-57 0.5164266882310855 1101 58-59 0.5566481057378354 1101 60-61 0.6300479632406351 1101 62-63 0.5751226372857019 1101 64-65 0.760015638121665 1101 66-67 0.6158842240001974 1101 68-69 0.8287977847858343 1101 70-71 0.6276749800306547 1101 72-73 0.620718422399996 1101 74-75 0.6332203934925325 1101 76-77 0.5140681813414396 1101 78-79 0.692993266394506 1101 80-81 0.8080231517139609 1101 82-83 0.7422126171226431 1101 84-85 0.772170419731804 1101 86-87 0.6561638296946697 1101 88-89 0.8157488444898675 1101 90-91 0.8151958281608387 1101 92-93 0.781779849423593 1101 94-95 0.7069377550279192 1101 96-97 0.4150672317320918 1101 98-99 0.3230631749050126 1101 100-101 0.5008543720655503 1102 1 0.1739470998682222 1102 2 0.2342104959277549 1102 3 0.1599021093325419 1102 4 0.13601813038532384 1102 5 0.20076904198583634 1102 6 0.18362908598003713 1102 7 0.16006979839109192 1102 8 0.20177530812262745 1102 9 0.20515042273954975 1102 10-11 0.18937760749395238 1102 12-13 0.1486529777982284 1102 14-15 0.23206776940892837 1102 16-17 0.27374558567320406 1102 18-19 0.27048682906170285 1102 20-21 0.2861283423886576 1102 22-23 0.3782196980806134 1102 24-25 0.2997137095111597 1102 26-27 0.29933171728261954 1102 28-29 0.17909874433427575 1102 30-31 0.2238208984579373 1102 32-33 0.2756263496780633 1102 34-35 0.34188896452819506 1102 36-37 0.3825570143319297 1102 38-39 0.4178380269173658 1102 40-41 0.42230785770487955 1102 42-43 0.36071415855257527 1102 44-45 0.4626249152581394 1102 46-47 0.385977461533372 1102 48-49 0.3432825731565643 1102 50-51 0.3961690310705066 1102 52-53 0.38991622571835194 1102 54-55 0.3006204417691407 1102 56-57 0.19849984087863248 1102 58-59 0.299230463778585 1102 60-61 0.2684593770156454 1102 62-63 0.2977485698023017 1102 64-65 0.20144703683952514 1102 66-67 0.2979719204845708 1102 68-69 0.24208914061362918 1102 70-71 0.06866863777710108 1102 72-73 0.2146952777372988 1102 74-75 0.07243399967964947 1102 76-77 0.06700575120888885 1102 78-79 0.0863535771193007 1102 80-81 0.44195591926352407 1102 82-83 0.4489740753846796 1102 84-85 0.4599881027560073 1102 86-87 0.30751811755997593 1102 88-89 0.3938344192851133 1102 90-91 0.5547014517777491 1102 92-93 0.4825911673803809 1102 94-95 0.4397198484278704 1102 96-97 0.030890355862315033 1102 98-99 -0.24802139608032192 1102 100-101 -0.47587891107729163 1103 1 0.23789453417154505 1103 2 0.2879795188344545 1103 3 0.2612877105226872 1103 4 0.1405666465615596 1103 5 0.20917124129009323 1103 6 0.21987133464061515 1103 7 0.2497338433831473 1103 8 0.2832225439293623 1103 9 0.25114027870890254 1103 10-11 0.23663556226864557 1103 12-13 0.19677406033975586 1103 14-15 0.2644789388596749 1103 16-17 0.2779121146269219 1103 18-19 0.23340177716576704 1103 20-21 0.3253047315893838 1103 22-23 0.28669187455681566 1103 24-25 0.30141337219992437 1103 26-27 0.380970041675063 1103 28-29 0.342015724891489 1103 30-31 0.2936770729556244 1103 32-33 0.35757951435957125 1103 34-35 0.36312192658742504 1103 36-37 0.35398024819662766 1103 38-39 0.33457117569635386 1103 40-41 0.3412682522211412 1103 42-43 0.3842585137083816 1103 44-45 0.49005780916370867 1103 46-47 0.4488747633853336 1103 48-49 0.35101748200198557 1103 50-51 0.37005666186456665 1103 52-53 0.3027561819997189 1103 54-55 0.32314477137302333 1103 56-57 0.29285875766849045 1103 58-59 0.1780292041894853 1103 60-61 0.24931680251673782 1103 62-63 0.2933716724503057 1103 64-65 0.2928371474163711 1103 66-67 0.3330613693174769 1103 68-69 0.31682690667029334 1103 70-71 0.2190948640966468 1103 72-73 0.3609253430759196 1103 74-75 0.2995710861751597 1103 76-77 0.37105285929398946 1103 78-79 0.37806426444177177 1103 80-81 0.40555724769009416 1103 82-83 0.42992153666074984 1103 84-85 0.35372803309035206 1103 86-87 0.313690506816922 1103 88-89 0.4107643741114657 1103 90-91 0.5690436378691608 1103 92-93 0.2960711374596343 1103 94-95 0.2978027626389732 1103 96-97 0.39227059289573774 1103 98-99 0.09090840146835077 1103 100-101 0.05893131288385334 1104 1 0.04500556876943307 1104 2 0.10348701017454687 1104 3 0.10647885446492467 1104 4 0.035557464974814934 1104 5 0.0532720718063473 1104 6 0.06761349899101532 1104 7 0.13494053342112267 1104 8 0.1292614196023294 1104 9 0.16939024740803887 1104 10-11 0.08014428477605406 1104 12-13 0.11980001918956162 1104 14-15 0.22431601786306032 1104 16-17 0.10894353026066739 1104 18-19 0.02496410761941803 1104 20-21 0.10006501913998278 1104 22-23 0.1435086810339996 1104 24-25 0.26706849928142873 1104 26-27 0.1958293442918162 1104 28-29 0.1322034257386946 1104 30-31 0.15930507262174132 1104 32-33 0.22004351836608294 1104 34-35 0.2716438476257821 1104 36-37 0.3375238189991805 1104 38-39 0.288470219976638 1104 40-41 0.3276132503430844 1104 42-43 0.2885156920398302 1104 44-45 0.41237689017034285 1104 46-47 0.40389542195551087 1104 48-49 0.40541186245355476 1104 50-51 0.3262537112364612 1104 52-53 0.6034870571718685 1104 54-55 0.7012918123980256 1104 56-57 0.6212087326277995 1104 58-59 0.6092416431528065 1104 60-61 0.6571201078879696 1104 62-63 0.6306476104328596 1104 64-65 0.5463050222963872 1104 66-67 0.45263458203814366 1104 68-69 0.4527639501993619 1104 70-71 0.4520058966078153 1104 72-73 0.28567545784575543 1104 74-75 0.2333658464105426 1104 76-77 0.30339865037115743 1104 78-79 0.4072588410454223 1104 80-81 0.5006766604858903 1104 82-83 0.43169525228196903 1104 84-85 0.34726440469420794 1104 86-87 0.14962963707025168 1104 88-89 0.4159681427354798 1104 90-91 0.3711504468504181 1104 92-93 0.319432015553204 1104 94-95 0.1991750446806222 1104 96-97 0.35180640108404404 1104 98-99 0.12260443877898197 1104 100-101 0.017706770919826198 1105 1 0.2028143768789903 1105 2 0.13202036807881967 1105 3 0.10770918700113441 1105 4 0.01932600388975203 1105 5 0.07719302195877731 1105 6 0.1291115547441848 1105 7 0.08211283997499663 1105 8 0.10166893573772029 1105 9 0.16697797422492755 1105 10-11 0.20631408280866026 1105 12-13 0.16180525051350259 1105 14-15 0.136835356761992 1105 16-17 0.1282841255916125 1105 18-19 0.07545916914676098 1105 20-21 0.15300547818794286 1105 22-23 0.07849051711156818 1105 24-25 0.20771843628268982 1105 26-27 0.2382380496891372 1105 28-29 0.14080450371713482 1105 30-31 0.18193979849865372 1105 32-33 0.1000737749038052 1105 34-35 0.20231621632126462 1105 36-37 0.06822188360931847 1105 38-39 0.2096835814236968 1105 40-41 0.3049117400875758 1105 42-43 0.2354493426525366 1105 44-45 0.15288047295582885 1105 46-47 0.17612712017869114 1105 48-49 0.23246058933615643 1105 50-51 0.324409998110724 1105 52-53 0.32263428137024164 1105 54-55 0.46569161368771006 1105 56-57 0.35002942407110993 1105 58-59 0.30689135087293096 1105 60-61 0.13368521944288858 1105 62-63 0.10441161729558246 1105 64-65 0.2607017165053591 1105 66-67 0.3358386143860628 1105 68-69 0.3612270309377479 1105 70-71 0.29034564766993753 1105 72-73 0.2651685465554081 1105 74-75 0.28123390690647554 1105 76-77 0.3041863131893301 1105 78-79 0.3344325297145012 1105 80-81 0.3227970930772379 1105 82-83 0.3717857361865775 1105 84-85 0.4916290174019551 1105 86-87 0.4637160339137445 1105 88-89 0.43300405201729575 1105 90-91 0.5178104600787279 1105 92-93 0.46760190929818535 1105 94-95 0.6379013594400433 1105 96-97 0.5357696861040573 1105 98-99 0.5008253565034835 1105 100-101 0.4718097424318266 1106 1 0.029671892230730634 1106 2 0.10327006531620953 1106 3 0.09480917428768976 1106 4 0.09144483222688393 1106 5 0.004874533956098048 1106 6 0.07929224760285081 1106 7 0.09058089046632034 1106 8 0.009519367736466222 1106 9 0.08558849454938411 1106 10-11 0.1357125128876433 1106 12-13 0.17446245019969808 1106 14-15 0.20391256320802142 1106 16-17 0.16048798930761876 1106 18-19 0.24817804454776393 1106 20-21 0.15511163786951698 1106 22-23 0.23501232514956172 1106 24-25 0.21482280609028948 1106 26-27 0.1339974437711433 1106 28-29 0.12791370739032715 1106 30-31 0.12490466356558727 1106 32-33 0.12429340535943822 1106 34-35 0.3429156397716753 1106 36-37 0.1384929556284007 1106 38-39 0.2779850168716109 1106 40-41 0.34132576980533713 1106 42-43 0.31448123617381185 1106 44-45 0.3279515080404636 1106 46-47 0.2683376713737431 1106 48-49 0.2326297574048013 1106 50-51 0.18603094862854164 1106 52-53 0.31931405655242173 1106 54-55 0.22354139106460025 1106 56-57 0.3511767350051187 1106 58-59 0.3404223742639516 1106 60-61 0.3987304107457774 1106 62-63 0.36634477075720184 1106 64-65 0.3502519189385538 1106 66-67 0.3832186799102715 1106 68-69 0.38311035044937114 1106 70-71 0.30220076331577417 1106 72-73 0.270030664243329 1106 74-75 0.24904080999728606 1106 76-77 0.30528383840863427 1106 78-79 0.3708970342226081 1106 80-81 0.41579663587555515 1106 82-83 0.5833892358639154 1106 84-85 0.526905210975702 1106 86-87 0.5692459864812243 1106 88-89 0.7897888974524605 1106 90-91 0.8296238177410231 1106 92-93 0.7501575461263847 1106 94-95 1.137808075825813 1106 96-97 1.1342089642422053 1106 98-99 0.9937365770321485 1106 100-101 0.8252984806964356 1107 1 0.06694675415047158 1107 2 0.06358299188706695 1107 3 0.03651755010512403 1107 4 0.06390649689527805 1107 5 0.033654346173584315 1107 6 0.036975089781179804 1107 7 0.03917949621271788 1107 8 0.053506441497731316 1107 9 0.14112349536308955 1107 10-11 0.11097513938510417 1107 12-13 0.12854922737485452 1107 14-15 0.10450739603375325 1107 16-17 0.15880943816228665 1107 18-19 0.2071083418313222 1107 20-21 0.2316864835152117 1107 22-23 0.19583489492036676 1107 24-25 0.1391838838968127 1107 26-27 0.09613040601204403 1107 28-29 0.1353242084647448 1107 30-31 0.1480375494181274 1107 32-33 0.07162163949307399 1107 34-35 0.23734742662823294 1107 36-37 0.19699564416649906 1107 38-39 0.20009612539954702 1107 40-41 0.21308482763797088 1107 42-43 0.2530429098095297 1107 44-45 0.28243699923421417 1107 46-47 0.24491417763592693 1107 48-49 0.2954992030578083 1107 50-51 0.32861297477992935 1107 52-53 0.3480307653433954 1107 54-55 0.3192555059726203 1107 56-57 0.35719929281948026 1107 58-59 0.35814945526550446 1107 60-61 0.3700445894214752 1107 62-63 0.2954498977445468 1107 64-65 0.3405318324536495 1107 66-67 0.4237114844590337 1107 68-69 0.40362909382766077 1107 70-71 0.5043618896122979 1107 72-73 0.5287987192960841 1107 74-75 0.49887491441021226 1107 76-77 0.4058529316788224 1107 78-79 0.544022281239311 1107 80-81 0.5013092515790056 1107 82-83 0.7525027655706857 1107 84-85 0.6532939015131767 1107 86-87 0.7262886610037107 1107 88-89 0.9529620698609875 1107 90-91 0.9940082438153617 1107 92-93 0.9685038710160363 1107 94-95 1.319741398752619 1107 96-97 1.0808229672246732 1107 98-99 0.9385152666728906 1107 100-101 0.975955586640449 1108 1 -0.005444887192325609 1108 2 0.03490310179105194 1108 3 0.07284249824378008 1108 4 0.0306776953211525 1108 5 -0.031427515462610245 1108 6 -0.04902380341195567 1108 7 0.045447841860088545 1108 8 0.09476962528268729 1108 9 0.07613322037659742 1108 10-11 0.06608716312643992 1108 12-13 0.09160420849116946 1108 14-15 0.019035854238850902 1108 16-17 -0.05225863045973966 1108 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-0.6662362755109044 2316 78-79 -0.6167900585072807 2316 80-81 -0.520293880631943 2316 82-83 -0.5791512009308555 2316 84-85 -0.5532022077250538 2316 86-87 -0.5691407208415136 2316 88-89 -1.0636286888771949 2316 90-91 -1.1568598450199765 2316 92-93 -0.7689270731873101 2316 94-95 -0.4719981371232471 2316 96-97 -0.4789521483307375 2316 98-99 -0.4640737221614337 2316 100-101 -0.1173006184723846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 21.0 5 48.0 6 118.0 7 318.0 8 677.0 9 1243.0 10 2029.0 11 3113.0 12 4432.0 13 5886.0 14 7523.0 15 9946.0 16 12707.0 17 17101.0 18 17064.0 19 13122.0 20 13255.0 21 14436.0 22 15717.0 23 17502.0 24 20128.0 25 23653.0 26 28282.0 27 34591.0 28 41676.0 29 50137.0 30 61617.0 31 76256.0 32 94460.0 33 122187.0 34 169371.0 35 246917.0 36 394606.0 37 575214.0 38 328115.0 39 15463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.372965842353246 18.346833388024386 14.60366257347311 23.67653819614926 2 16.35118163893129 19.906451278279157 38.076371608520574 25.665995474268975 3 20.468457032411173 22.719760158396486 28.628508333323083 28.183274475869258 4 14.080689872621049 15.617995172498905 34.76296904027688 35.53834591460317 5 15.022778310112209 36.464607823963085 32.91848148972731 15.594132376197395 6 34.777893538307374 34.07681114318785 15.997790846280541 15.147504472224238 7 30.838605084251665 31.138448090012766 19.200325714869106 18.822621110866468 8 27.424995172088895 34.04417405408818 18.625404529749158 19.905426244073766 9 26.73645919564336 16.791413329462802 19.199505687504793 37.27262178738904 10-11 26.412732892896635 25.420151270447892 25.709764434839187 22.45735140181628 12-13 27.265212840152476 22.42627236470881 26.42866192444842 23.879852870690296 14-15 23.70229399655871 24.04847330333495 24.74366105015377 27.50557164995257 16-17 23.782180503366554 27.293675454919875 25.269130559303232 23.65501348241034 18-19 23.86636831331612 26.420385064428537 26.787121877617043 22.9261247446383 20-21 24.478559867647558 25.030335893527628 26.794276617837443 23.696827620987374 22-23 24.18400102011404 24.74024608201176 27.06057501138813 24.01517788648607 24-25 24.412071130813633 24.981416129856253 26.874695308582446 23.731817430747665 26-27 23.849311740641692 25.121256446185182 27.35714351609355 23.672288297079575 28-29 23.68464535661555 26.00487178257139 26.87994348371405 23.430539377099013 30-31 23.75980086455485 25.175086092622912 26.768829775849618 24.29628326697262 32-33 23.870914572419284 25.265945124588807 26.82908128644253 24.034059016549385 34-35 24.56588461215964 25.078575495106087 26.709702523251806 23.64583736948247 36-37 24.2017139584962 24.867774984669342 27.06741451171209 23.86309654512237 38-39 23.89637776059319 25.349607483441154 26.9379608164082 23.816053939557452 40-41 24.05634654053185 25.321562591230702 26.79351355872715 23.828577309510298 42-43 23.753093050860315 25.326525717167534 26.35576593882166 24.564615293150492 44-45 23.994444245129745 25.11678873268311 26.612803862377188 24.275963159809958 46-47 24.486486929745976 25.528499119492732 26.13515735351701 23.84985659724429 48-49 24.356675117090234 25.440408113226653 26.115457960885323 24.087458808797788 50-51 23.785559476199104 25.809080330733575 26.58755312754465 23.817807065522672 52-53 24.356380230755985 25.888869754893516 25.737267009438526 24.01748300491197 54-55 23.97846116124895 25.58647332061471 25.91757986964025 24.51748564849609 56-57 24.177383141090086 25.619309990799223 25.965220728485182 24.238086139625505 58-59 24.572070899263068 25.3027905647512 25.809167012793075 24.315971523192655 60-61 24.064434892885274 25.554803403299893 26.498579088262996 23.88218261555184 62-63 23.819754719402557 25.703702579952363 26.49807025319803 23.978472447447047 64-65 23.612636134848636 26.030897823728488 26.122002901258007 24.234463140164873 66-67 23.906062962407923 25.627226512921748 25.85568721384754 24.611023310822794 68-69 23.743161870648137 25.471626939708013 26.230658272711693 24.554552916932153 70-71 24.063830943123914 25.513188605307423 25.99979294304806 24.4231875085206 72-73 24.01642842821665 25.284067729340126 26.186507843766748 24.512995998676477 74-75 23.44283978756371 26.22119500127719 25.833363059325286 24.50260215183381 76-77 23.89675292856344 25.555498003775394 25.839618809894958 24.7081302577662 78-79 23.84719339024059 25.545841859923517 26.1019033276355 24.50506142220039 80-81 24.067925382368824 25.452706728043033 25.97713515825106 24.502232731337084 82-83 23.841557592776873 25.41445208026592 26.23741104240648 24.506579284550725 84-85 23.589276174145933 25.642030174546925 26.062642710387244 24.706050940919898 86-87 23.730495024593306 25.733320171478987 25.896598310535072 24.63958649339263 88-89 23.526547830336703 25.788162666872445 25.616400442420968 25.068889060369887 90-91 23.712377201492238 25.216312653784012 25.88129071569582 25.190019429027927 92-93 23.77970702882355 25.39924057265791 25.96989761548343 24.85115478303511 94-95 23.55840214387954 25.25290668949623 26.059055090668377 25.12963607595585 96-97 23.331029056439615 25.253685715492324 26.283845091910717 25.131440136157345 98-99 23.27747031294717 25.5890053966023 25.664693953361745 25.46883033708879 100-101 23.492277676059903 24.561412140665468 26.307891291098105 25.638418892176524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 397.0 1 275.0 2 213.0 3 272.0 4 240.0 5 245.5 6 423.0 7 952.0 8 1539.5 9 1752.0 10 1624.5 11 1460.5 12 1511.0 13 1664.0 14 1843.5 15 1912.5 16 1972.0 17 2154.5 18 2346.5 19 2511.0 20 2711.0 21 3077.0 22 3554.0 23 4198.5 24 5042.0 25 6159.0 26 7580.0 27 9352.5 28 11382.5 29 13326.5 30 15606.0 31 19131.5 32 23363.5 33 25793.5 34 27281.5 35 29502.0 36 32877.5 37 37457.0 38 41840.5 39 45797.5 40 50195.5 41 54967.5 42 58580.0 43 63582.0 44 71264.0 45 77069.0 46 80549.0 47 92898.5 48 113807.0 49 116392.5 50 105498.5 51 101244.5 52 102259.5 53 105627.0 54 108289.5 55 104049.5 56 95906.5 57 90286.5 58 84924.5 59 76592.0 60 63590.5 61 51976.5 62 42510.5 63 32651.0 64 24552.5 65 19047.0 66 14648.5 67 11225.0 68 8554.0 69 6511.0 70 4918.5 71 3555.0 72 2670.5 73 2199.5 74 1804.5 75 1456.5 76 1057.0 77 661.0 78 433.5 79 290.0 80 191.5 81 125.0 82 72.0 83 43.5 84 26.5 85 16.5 86 10.0 87 5.5 88 4.0 89 3.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2911507156993829 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013735458352245216 16-17 0.0010045335212836052 18-19 2.0500684107828678E-5 20-21 2.0500684107828678E-5 22-23 0.0 24-25 0.0 26-27 2.0500684107828678E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001906563622028067 36-37 0.008302777063670615 38-39 0.002665088934017728 40-41 0.002911097143311672 42-43 3.2801094572525885E-4 44-45 0.0012505417305775494 46-47 3.2801094572525885E-4 48-49 4.920164185878883E-4 50-51 4.1001368215657356E-5 52-53 1.2300410464697207E-4 54-55 0.0 56-57 7.790259960974898E-4 58-59 0.0018860629379202382 60-61 6.560218914505177E-4 62-63 9.020301007444619E-4 64-65 4.1001368215657356E-5 66-67 4.7151573448005955E-4 68-69 0.001845061569704581 70-71 2.0500684107828678E-5 72-73 0.0 74-75 0.0 76-77 0.26039968953763987 78-79 1.6400547286262942E-4 80-81 8.200273643131471E-5 82-83 0.0 84-85 0.0 86-87 0.10699307035875788 88-89 0.3743629924930595 90-91 0.2770667457173046 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.1001368215657356E-5 100-101 3.075102616174302E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2438943.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.728932077576566 #Duplication Level Percentage of deduplicated Percentage of total 1 68.35363258706279 29.890313566552933 2 15.46261403987792 13.523271981832066 3 6.1533352789191 8.072363413872269 4 3.0840331867025483 5.39445910985231 5 1.7944711000196667 3.923515242396706 6 1.1222093412269145 2.944380963560021 7 0.7377592755711844 2.2582997675738086 8 0.520870781468818 1.8221698419235335 9 0.4083847622368177 1.607240657643395 >10 2.135163757302384 16.98460103363312 >50 0.15181542336386947 4.52642309821394 >100 0.07064322687914792 5.5497440418561705 >500 0.0027212952166184696 0.8061589343738833 >1k 0.002064430853985882 1.8191715381039502 >5k 1.876755321805347E-4 0.44095408270284103 >10k+ 9.383776609026734E-5 0.43693272590916166 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10648 0.43658256876031953 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5515 0.2261225457093503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5231 0.21447815713610363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4419 0.18118504614498984 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4039 0.16560452622304006 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3742 0.15342711986298985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3169 0.12993333587541817 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2992 0.12267609370124682 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2606 0.10684956557000308 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2598 0.10652155462427783 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.200273643131471E-5 2 0.0 0.0 0.0 0.0 8.200273643131471E-5 3 0.0 0.0 0.0 0.0 8.200273643131471E-5 4 0.0 0.0 0.0 0.0 8.200273643131471E-5 5 0.0 0.0 0.0 0.0 1.2300410464697207E-4 6 0.0 0.0 0.0 0.0 2.4600820929394413E-4 7 0.0 0.0 0.0 0.0 2.4600820929394413E-4 8 0.0 0.0 0.0 0.0 2.4600820929394413E-4 9 0.0 0.0 0.0 4.1001368215657356E-5 2.870095775096015E-4 10-11 0.0 0.0 0.0 1.845061569704581E-4 2.870095775096015E-4 12-13 0.0 0.0 0.0 2.0500684107828678E-4 4.5101505037223094E-4 14-15 0.0 2.0500684107828678E-5 0.0 2.2550752518611547E-4 4.715157344800596E-4 16-17 0.0 4.1001368215657356E-5 0.0 2.4600820929394413E-4 5.535184709113744E-4 18-19 0.0 4.1001368215657356E-5 0.0 3.2801094572525885E-4 5.74019155019203E-4 20-21 0.0 4.1001368215657356E-5 0.0 4.305143662644023E-4 5.945198391270317E-4 22-23 0.0 4.1001368215657356E-5 0.0 5.945198391270317E-4 6.35521207342689E-4 24-25 0.0 4.1001368215657356E-5 0.0 7.58525311989661E-4 6.560218914505177E-4 26-27 0.0 4.1001368215657356E-5 0.0 0.0011890396782540634 7.175239437740037E-4 28-29 0.0 4.1001368215657356E-5 0.0 0.0032801094572525885 7.790259960974898E-4 30-31 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.009327811269062049 7.790259960974898E-4 32-33 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.021505217629112283 8.200273643131471E-4 34-35 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.03876679364790403 8.405280484209758E-4 36-37 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.06699623566438412 8.610287325288044E-4 38-39 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.10639855051963085 8.610287325288044E-4 40-41 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.15232008292116705 8.610287325288044E-4 42-43 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.20146022272763242 8.815294166366332E-4 44-45 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.25994867448726766 9.020301007444619E-4 46-47 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.3330541140157847 9.020301007444619E-4 48-49 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.40864013632134905 9.020301007444619E-4 50-51 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.4957680437796209 9.430314689601192E-4 52-53 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.5822194286623344 0.0010250342053914339 54-55 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.6777731172889239 0.0010660355736070912 56-57 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.7953035392791057 0.0010660355736070912 58-59 4.1001368215657356E-5 4.1001368215657356E-5 0.0 0.9215467520151148 0.0010660355736070912 60-61 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.0583683177507632 0.0010660355736070912 62-63 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.2034926605500824 0.0010660355736070912 64-65 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.3546237037929956 0.0010865362577149199 66-67 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.5370592916685628 0.0011070369418227485 68-69 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.745018231258377 0.0011070369418227485 70-71 4.1001368215657356E-5 4.1001368215657356E-5 0.0 1.9737853652176374 0.001148038310038406 72-73 4.1001368215657356E-5 4.1001368215657356E-5 0.0 2.2010149478688104 0.0014145472034401787 74-75 8.200273643131471E-5 4.1001368215657356E-5 0.0 2.439827417040907 0.0014350478875480074 76-77 8.200273643131471E-5 6.150205232348603E-5 0.0 2.7024616811463 0.001455548571655836 78-79 8.200273643131471E-5 8.200273643131471E-5 0.0 3.008905907190123 0.001476049255763665 80-81 8.200273643131471E-5 8.200273643131471E-5 0.0 3.3461626614480124 0.0014965499398714936 82-83 8.200273643131471E-5 8.200273643131471E-5 0.0 3.6862280094286746 0.0016810560968419516 84-85 8.200273643131471E-5 8.200273643131471E-5 0.0 4.045112985420324 0.0016810560968419516 86-87 8.200273643131471E-5 8.200273643131471E-5 0.0 4.4175079122390315 0.0017425581491654378 88-89 8.200273643131471E-5 8.200273643131471E-5 0.0 4.82889514023083 0.0018040602014889238 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8825 0.0 25.026644 1 TACATGG 8965 0.0 24.138102 2 ACATGGG 9375 0.0 22.221931 3 GTAAGGT 730 0.0 20.802505 4 TAAGGTG 740 0.0 20.52139 5 ACCGGTC 140 4.8845075E-4 20.338165 8 GTATAGG 535 0.0 19.573488 1 AGTACTT 6435 0.0 19.505886 12-13 AGAGTAC 11150 0.0 19.47174 10-11 GAGTACT 6160 0.0 19.028511 12-13 CATGGGG 6325 0.0 18.982286 4 TGTACGC 175 1.0014006E-4 18.982286 2 TCGTCCG 230 2.0782354E-6 18.56963 9 CGTAAAC 155 9.689243E-4 18.369955 3 CATGGGA 3575 0.0 18.318571 4 TACTTTT 6875 0.0 17.912376 14-15 GTACTTT 6585 0.0 17.548166 14-15 AGGACCG 1085 0.0 17.495193 5 ACCGAGT 870 0.0 16.36404 8 GGACCGA 760 0.0 16.23485 6 >>END_MODULE