FastQCFastQC Report
Fri 27 May 2016
SRR939238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487538
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103370.4155514408222105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57700.2319562555426289No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55710.22395637775181726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44160.1775249262523829No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42380.17036925667065186No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39650.15939454995260374No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30790.12377700360758306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.11834995083492192No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25660.10315420307147066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93550.025.6918871
TACATGG94800.024.3579082
ACATGGG98650.023.022783
GAGTACT62300.021.67817512-13
AGTACTT66350.020.56920212-13
AGAGTAC111850.020.3782210-11
GTACTTT67200.019.99210214-15
TACTTTT70650.019.18352914-15
CATGGGG67650.018.9941944
GTCTTAG6750.018.2961121
TAAGGTG8350.018.171175
ACTTTTT75850.017.71378716-17
GAGTACA74450.017.6089571
TAGACAG13100.017.3735795
GTATAGG5000.017.0998291
CATGGGA39650.016.8614644
GTATCAA193550.016.5407811
AGTACAT74250.016.475652
GTAAGGT7200.015.8051294
TAGACTG6750.015.4539055
Produced by FastQC (version 0.11.5)