##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2487538 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91991961529834 33.0 31.0 34.0 30.0 34.0 2 32.00943663976189 34.0 31.0 34.0 30.0 34.0 3 32.071773777928215 34.0 31.0 34.0 30.0 34.0 4 35.552121816832546 37.0 35.0 37.0 33.0 37.0 5 35.58648028693431 37.0 35.0 37.0 33.0 37.0 6 35.670192776954565 37.0 35.0 37.0 33.0 37.0 7 35.584902421591146 37.0 35.0 37.0 33.0 37.0 8 35.564431980536575 37.0 35.0 37.0 33.0 37.0 9 37.20209259114836 39.0 37.0 39.0 34.0 39.0 10-11 37.11536487080801 39.0 37.0 39.0 33.0 39.0 12-13 37.01442470426582 39.0 37.0 39.0 33.0 39.0 14-15 38.277087023394216 40.0 38.0 41.0 33.0 41.0 16-17 38.21855907326843 40.0 38.0 41.0 33.0 41.0 18-19 38.129332496629196 40.0 38.0 41.0 33.0 41.0 20-21 38.00617256902206 40.0 38.0 41.0 33.0 41.0 22-23 37.92996408497076 40.0 38.0 41.0 33.0 41.0 24-25 37.95282403726094 40.0 38.0 41.0 33.0 41.0 26-27 37.867197807631484 40.0 38.0 41.0 33.0 41.0 28-29 37.73281654390807 40.0 38.0 41.0 32.5 41.0 30-31 37.57766132617874 40.0 37.5 41.0 32.0 41.0 32-33 37.38990600344598 40.0 37.0 41.0 31.5 41.0 34-35 37.08790217476075 40.0 37.0 41.0 30.5 41.0 36-37 36.84464197129853 40.0 36.0 41.0 30.0 41.0 38-39 36.52364968896958 39.5 35.5 41.0 30.0 41.0 40-41 36.40414015785889 39.0 36.0 41.0 30.0 41.0 42-43 36.23144370055854 39.0 35.0 41.0 30.0 41.0 44-45 36.23921142109186 39.0 35.0 41.0 30.0 41.0 46-47 36.0861438096624 39.0 35.0 41.0 28.5 41.0 48-49 35.88828271166109 39.0 35.0 41.0 28.0 41.0 50-51 34.71702060430836 38.0 34.0 40.0 25.5 40.5 52-53 34.81906628160053 38.0 34.5 39.5 26.5 40.5 54-55 35.29821855987728 39.0 35.0 40.5 27.0 41.0 56-57 35.14780558126147 38.5 35.0 40.5 26.5 41.0 58-59 34.90405312401258 38.0 34.5 40.0 26.0 41.0 60-61 34.56191885309893 37.5 34.0 40.0 26.0 41.0 62-63 34.1818904877031 37.0 34.0 40.0 25.5 41.0 64-65 33.763342509742564 36.5 34.0 39.5 24.5 41.0 66-67 33.357734836613545 36.0 33.0 39.0 23.0 41.0 68-69 32.915154059958084 35.0 33.0 39.0 22.0 40.5 70-71 32.479100218770526 35.0 32.5 37.5 21.5 40.0 72-73 32.04408676369969 35.0 32.0 37.0 20.0 39.0 74-75 31.607746494726914 35.0 32.0 36.5 19.5 39.0 76-77 31.1814191381197 35.0 31.0 36.0 18.0 39.0 78-79 30.781964536823153 35.0 31.0 35.5 15.5 37.5 80-81 30.35260144769648 34.0 31.0 35.0 9.5 37.0 82-83 29.947675975201182 34.0 30.0 35.0 7.0 36.5 84-85 29.56880296904007 34.0 30.0 35.0 2.0 36.0 86-87 29.278510720238245 34.0 29.5 35.0 2.0 36.0 88-89 28.884993314674993 34.0 29.0 35.0 2.0 35.0 90-91 28.503479946839 34.0 29.0 35.0 2.0 35.0 92-93 28.101350009527494 34.0 29.0 35.0 2.0 35.0 94-95 27.63248501128425 34.0 27.0 35.0 2.0 35.0 96-97 27.067211435563998 33.5 26.5 35.0 2.0 35.0 98-99 26.344410216044942 33.0 25.0 35.0 2.0 35.0 100-101 23.899177620603183 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41963158705446446 1101 2 0.21161953407315082 1101 3 0.3571404883972207 1101 4 0.42172007630206565 1101 5 0.48654670841015246 1101 6 0.35571025346533247 1101 7 0.4252995430395927 1101 8 0.4486062815623555 1101 9 0.36496662710510464 1101 10-11 0.45166979285229303 1101 12-13 0.47686541268870286 1101 14-15 0.591509125736394 1101 16-17 0.6874558640049315 1101 18-19 0.6753294328012913 1101 20-21 0.6738640994570773 1101 22-23 0.6559200974983384 1101 24-25 0.6648939175607964 1101 26-27 0.5874212684418225 1101 28-29 0.6276238885282766 1101 30-31 0.7051752256005273 1101 32-33 0.769994593801897 1101 34-35 0.7450030096137681 1101 36-37 0.5701488338104994 1101 38-39 0.7477739497017524 1101 40-41 0.6905919349623844 1101 42-43 0.5844696489106411 1101 44-45 0.69592995350731 1101 46-47 0.6769420544353011 1101 48-49 0.7570443164355183 1101 50-51 0.6630809026085203 1101 52-53 0.6448280972412874 1101 54-55 0.6087705733306663 1101 56-57 0.560864170564507 1101 58-59 0.5203410113401148 1101 60-61 0.5590677854225916 1101 62-63 0.6535642109062394 1101 64-65 0.4579576090044739 1101 66-67 0.4398331169539631 1101 68-69 0.3846138879103549 1101 70-71 0.41192498292294033 1101 72-73 0.45065226314964946 1101 74-75 0.3908862580252723 1101 76-77 0.14621436738040217 1101 78-79 0.13448369942384275 1101 80-81 0.12302090538817012 1101 82-83 0.0743015336934576 1101 84-85 0.2359081726391956 1101 86-87 0.08225254234260859 1101 88-89 0.11847043899749465 1101 90-91 0.09934781808063065 1101 92-93 0.08349776324739722 1101 94-95 0.02288200560680309 1101 96-97 -0.036999748315245995 1101 98-99 -0.13364562817906744 1101 100-101 -0.42776829222453827 1102 1 0.32268503055683695 1102 2 0.3880849402902271 1102 3 0.4604305930002184 1102 4 0.4211253923484506 1102 5 0.366177295815433 1102 6 0.3660030789510742 1102 7 0.29439253539300836 1102 8 0.3596550888411798 1102 9 0.3226046311040207 1102 10-11 0.4333405876372538 1102 12-13 0.5105319319631647 1102 14-15 0.47691007988301237 1102 16-17 0.4573611048396202 1102 18-19 0.3990383017574999 1102 20-21 0.5104747305647095 1102 22-23 0.4398972202586151 1102 24-25 0.5652851131312957 1102 26-27 0.5317726612981133 1102 28-29 0.4548500127916526 1102 30-31 0.4730462456795905 1102 32-33 0.364206721868662 1102 34-35 0.3551289484812443 1102 36-37 0.2560624968401015 1102 38-39 0.4382300584643204 1102 40-41 0.36078161622012317 1102 42-43 0.2722525216338809 1102 44-45 0.3239441524155424 1102 46-47 0.35099291876362315 1102 48-49 0.3009485312083058 1102 50-51 0.2840895199370763 1102 52-53 0.3400245323961073 1102 54-55 0.4207969243279521 1102 56-57 0.37426318246841817 1102 58-59 0.27286960220627776 1102 60-61 0.2236956845305471 1102 62-63 0.2263231011377016 1102 64-65 0.19917507994377814 1102 66-67 0.10817139683014432 1102 68-69 0.16525403610582856 1102 70-71 0.09693212708729249 1102 72-73 0.04603689806220501 1102 74-75 -0.21301037116436916 1102 76-77 -0.09989072742612493 1102 78-79 -0.16888373872163953 1102 80-81 -0.0829164995315459 1102 82-83 -0.18999257323294927 1102 84-85 -0.21395801873498854 1102 86-87 -0.3922490806766419 1102 88-89 -0.2662543676402329 1102 90-91 -0.17921860900740327 1102 92-93 0.03630829379490663 1102 94-95 0.013200725136119473 1102 96-97 -0.24099528941702175 1102 98-99 -0.4163214180061239 1102 100-101 -0.5936127453331714 1103 1 0.1859027548224539 1103 2 0.32788319704805247 1103 3 0.27296110979408894 1103 4 0.29804145594182074 1103 5 0.2548889033144377 1103 6 0.23025488258436866 1103 7 0.17473150783878566 1103 8 0.23806947813100265 1103 9 0.3297543597629371 1103 10-11 0.41069195897957655 1103 12-13 0.33997392016650707 1103 14-15 0.43902651800193837 1103 16-17 0.3316556571240312 1103 18-19 0.38777404336168075 1103 20-21 0.31250886691569946 1103 22-23 0.35380522462509134 1103 24-25 0.4292282572018564 1103 26-27 0.41900409954553197 1103 28-29 0.37705160184370357 1103 30-31 0.4297559738468877 1103 32-33 0.4582233073620543 1103 34-35 0.3265026595133236 1103 36-37 0.27314128687226713 1103 38-39 0.5375622923550836 1103 40-41 0.5830229259869384 1103 42-43 0.4528714385265218 1103 44-45 0.382894497611467 1103 46-47 0.558427508076015 1103 48-49 0.5954709273227152 1103 50-51 0.5145540232493673 1103 52-53 0.39864722359464366 1103 54-55 0.4632003236613613 1103 56-57 0.38882381326241955 1103 58-59 0.3098822133935428 1103 60-61 0.3088589081766102 1103 62-63 0.29946200967279424 1103 64-65 0.42353426917191683 1103 66-67 0.30299060891864826 1103 68-69 0.3259304726158092 1103 70-71 0.1357413883266929 1103 72-73 0.22994073482626476 1103 74-75 0.1963317063128116 1103 76-77 0.27991835615944183 1103 78-79 0.23116447362273362 1103 80-81 0.13811866363623437 1103 82-83 0.16179133937477275 1103 84-85 0.17394409065284222 1103 86-87 0.27173322544884115 1103 88-89 0.2374565303607703 1103 90-91 0.3376769405965625 1103 92-93 0.28098454482982405 1103 94-95 0.14314723904283966 1103 96-97 0.02865221856902167 1103 98-99 -0.2102893442272702 1103 100-101 -0.3845979866574609 1104 1 0.32736590799772713 1104 2 0.3279769954184957 1104 3 0.33335893077465073 1104 4 0.37202591655773176 1104 5 0.44399655849279185 1104 6 0.2870688090710729 1104 7 0.38664230739927063 1104 8 0.35807815538565535 1104 9 0.4247246666031188 1104 10-11 0.45703903757681275 1104 12-13 0.5027035903656412 1104 14-15 0.4826012934109798 1104 16-17 0.5083357525303782 1104 18-19 0.3470686289141227 1104 20-21 0.6150759725877109 1104 22-23 0.5957188272720231 1104 24-25 0.5523000488086751 1104 26-27 0.5237305233794487 1104 28-29 0.43989614788494436 1104 30-31 0.5029406132229326 1104 32-33 0.5574208785999346 1104 34-35 0.6370326673865918 1104 36-37 0.7002101566055288 1104 38-39 0.7499031520117185 1104 40-41 0.5052358287314291 1104 42-43 0.4823136243255206 1104 44-45 0.5609862766007723 1104 46-47 0.571868923207326 1104 48-49 0.6179358181218575 1104 50-51 0.5697364375397527 1104 52-53 0.6900921603296339 1104 54-55 0.6682823121288664 1104 56-57 0.5795863396907421 1104 58-59 0.707561228851084 1104 60-61 0.6497270754723417 1104 62-63 0.6076557500258701 1104 64-65 0.7014968671622412 1104 66-67 0.7278369280750354 1104 68-69 0.6538553701800183 1104 70-71 0.5448000655451324 1104 72-73 0.5859763521318158 1104 74-75 0.4371861841166442 1104 76-77 0.6030977442753702 1104 78-79 0.4528676057639913 1104 80-81 0.47395684804745386 1104 82-83 0.4387880676586384 1104 84-85 0.40335027815831737 1104 86-87 0.30486342555444423 1104 88-89 0.4464099650832587 1104 90-91 0.4087123659594951 1104 92-93 0.4446552770582919 1104 94-95 0.2017565532572405 1104 96-97 0.08960323264167513 1104 98-99 0.04215412143057762 1104 100-101 -0.06271602194737724 1105 1 0.23674938417821423 1105 2 0.2666758361702861 1105 3 0.20579399190090442 1105 4 0.2672367746004838 1105 5 0.26759886632456187 1105 6 0.21940547096234297 1105 7 0.36306168437000963 1105 8 0.2854553962967046 1105 9 0.3069847207084777 1105 10-11 0.362284271363869 1105 12-13 0.3235977863038286 1105 14-15 0.36304853357549405 1105 16-17 0.255181680231118 1105 18-19 0.2834799007455686 1105 20-21 0.30449058956656216 1105 22-23 0.3525731800517633 1105 24-25 0.23651297934976157 1105 26-27 0.3271183294106663 1105 28-29 0.3898751740595685 1105 30-31 0.38991142405679824 1105 32-33 0.39862538050712004 1105 34-35 0.3387127063481401 1105 36-37 0.49218340253607806 1105 38-39 0.5632640120096539 1105 40-41 0.46247499788356095 1105 42-43 0.2767994634385005 1105 44-45 0.40883770370962225 1105 46-47 0.4958860861952701 1105 48-49 0.41737592363086407 1105 50-51 0.30281624827763665 1105 52-53 0.29146630138038887 1105 54-55 0.4201863088868123 1105 56-57 0.4683436583115892 1105 58-59 0.5145894155394544 1105 60-61 0.5485953776233998 1105 62-63 0.4785140416189222 1105 64-65 0.28474002489607386 1105 66-67 0.4894907319804247 1105 68-69 0.42954706205675564 1105 70-71 0.3129726410242384 1105 72-73 0.39159058305007477 1105 74-75 0.4712659841898734 1105 76-77 0.4081415937899493 1105 78-79 0.40182993464068417 1105 80-81 0.4158789036245345 1105 82-83 0.32592382518699736 1105 84-85 0.26583610305176464 1105 86-87 0.20130925087447338 1105 88-89 0.3154147392946989 1105 90-91 0.2337839080333417 1105 92-93 0.18173557837040377 1105 94-95 0.027693176755743565 1105 96-97 -0.04252653490435421 1105 98-99 -0.058977398674080916 1105 100-101 -0.10702233933468008 1106 1 0.16156073309239005 1106 2 0.2804416487358523 1106 3 0.25085013774569376 1106 4 0.26691559209026394 1106 5 0.2437880707519966 1106 6 0.29855583848939915 1106 7 0.2563824846032148 1106 8 0.24790060762257582 1106 9 0.3371367761892685 1106 10-11 0.33578854710603423 1106 12-13 0.33967916766791717 1106 14-15 0.36361069024623305 1106 16-17 0.36991453144156594 1106 18-19 0.47348806995289294 1106 20-21 0.4703828398166081 1106 22-23 0.3896153858773843 1106 24-25 0.38105662962389886 1106 26-27 0.45914938985406906 1106 28-29 0.45098459779879363 1106 30-31 0.40538220954005055 1106 32-33 0.40104585728860087 1106 34-35 0.392672328120824 1106 36-37 0.4183136944106778 1106 38-39 0.5986214061023389 1106 40-41 0.6736751905930589 1106 42-43 0.43919038793792 1106 44-45 0.4644094347611656 1106 46-47 0.41839286332079695 1106 48-49 0.5678322122622248 1106 50-51 0.5302439254822318 1106 52-53 0.4591984561135547 1106 54-55 0.5584189846687764 1106 56-57 0.6118789002370804 1106 58-59 0.6582842013857828 1106 60-61 0.5446790456673156 1106 62-63 0.5019751148223506 1106 64-65 0.6001363623681257 1106 66-67 0.5061612388727639 1106 68-69 0.5659542342018469 1106 70-71 0.4182367176332562 1106 72-73 0.3237037719560121 1106 74-75 0.5033323048871203 1106 76-77 0.46710433138209595 1106 78-79 0.4146411020169225 1106 80-81 0.5911441846679573 1106 82-83 0.5731161838361487 1106 84-85 0.5408157236723774 1106 86-87 0.5200599381875932 1106 88-89 0.36254495611035154 1106 90-91 0.5401220259387465 1106 92-93 0.5590313787345238 1106 94-95 0.5348234284825395 1106 96-97 0.506119523714947 1106 98-99 0.4457571631823569 1106 100-101 0.1801296620338384 1107 1 0.1237081201163015 1107 2 0.11392453212847187 1107 3 0.1464655459397548 1107 4 0.055989614899139895 1107 5 0.16245291346464086 1107 6 0.08498485805000655 1107 7 0.09514339699185115 1107 8 0.1255823235969018 1107 9 0.2228059667345974 1107 10-11 0.16935198340707558 1107 12-13 0.21681059340715336 1107 14-15 0.2610810927459184 1107 16-17 0.30721307536551024 1107 18-19 0.34932331082971046 1107 20-21 0.25882188640081694 1107 22-23 0.22208296522958904 1107 24-25 0.16974062452857197 1107 26-27 0.09762329771267986 1107 28-29 0.11385304722362832 1107 30-31 0.17377302897147473 1107 32-33 0.26313674762344164 1107 34-35 0.329026420824313 1107 36-37 0.33390529605723174 1107 38-39 0.4468576179327073 1107 40-41 0.2137308201785686 1107 42-43 0.26792284279751044 1107 44-45 0.5107989690573831 1107 46-47 0.4389076225536357 1107 48-49 0.6195842928468451 1107 50-51 0.5332167917200366 1107 52-53 0.5265179977767858 1107 54-55 0.5557954059636927 1107 56-57 0.6265655932846173 1107 58-59 0.6009923581142473 1107 60-61 0.5121931964093918 1107 62-63 0.4862030240517896 1107 64-65 0.5253914451722963 1107 66-67 0.3772276632227829 1107 68-69 0.3290527697246759 1107 70-71 0.3807557445676153 1107 72-73 0.3510586118193615 1107 74-75 0.34851897760663775 1107 76-77 0.39038962144257283 1107 78-79 0.4568986516801061 1107 80-81 0.434318215557429 1107 82-83 0.4363257052405274 1107 84-85 0.4430916343377653 1107 86-87 0.47467693567788416 1107 88-89 0.43586438204730626 1107 90-91 0.5903360942385056 1107 92-93 0.5704697230323283 1107 94-95 0.5601621682234281 1107 96-97 0.5342270904418527 1107 98-99 0.6302633046879222 1107 100-101 0.6495300160617603 1108 1 0.1656190038391081 1108 2 0.2041358212919846 1108 3 0.15573996550371305 1108 4 0.23057961164273166 1108 5 0.2751181290073035 1108 6 0.29258380519892313 1108 7 0.29145129379786994 1108 8 0.24621809418310647 1108 9 0.33993207105872614 1108 10-11 0.31777880645073253 1108 12-13 0.30805467795145347 1108 14-15 0.3148563140499192 1108 16-17 0.23877011016678296 1108 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2316 68-69 -0.8743874903661251 2316 70-71 -0.7342976079601407 2316 72-73 -0.990737859191988 2316 74-75 -0.923353939010159 2316 76-77 -0.6599286474818022 2316 78-79 -0.7358969760515386 2316 80-81 -0.567040592859545 2316 82-83 -0.49556014367178847 2316 84-85 -0.47154826315565757 2316 86-87 -0.3586711857492375 2316 88-89 -0.6442218179663683 2316 90-91 -0.5544596745414552 2316 92-93 -0.541466304103988 2316 94-95 -0.44226109626237076 2316 96-97 -0.241160064959125 2316 98-99 -0.31986214087208964 2316 100-101 0.059673496884709465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8616.0 3 2486.0 4 2574.0 5 2710.0 6 3090.0 7 3694.0 8 4444.0 9 5414.0 10 6428.0 11 7779.0 12 9348.0 13 12946.0 14 11588.0 15 11841.0 16 14492.0 17 18330.0 18 16248.0 19 13304.0 20 13579.0 21 14724.0 22 16097.0 23 18096.0 24 20842.0 25 23719.0 26 27386.0 27 32170.0 28 38370.0 29 45313.0 30 55353.0 31 68292.0 32 85277.0 33 111377.0 34 153958.0 35 226531.0 36 359099.0 37 541994.0 38 423299.0 39 56722.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33638704563716 17.649107854516807 14.0907260169757 23.92377908287033 2 15.861370072072623 19.33609313901468 39.13400968158937 25.66852710732333 3 20.416200261710035 22.61195630867294 28.386442116075767 28.585401313541258 4 13.614966593718517 15.108719846498953 35.11622262482123 36.160090934961296 5 15.126982018428395 36.24508395022965 32.9267654505859 15.701168580756056 6 34.654237967172335 33.76428054836662 16.374291352550248 15.207190131910798 7 30.888387031372446 30.970651794377513 19.361585211812656 18.779375962437385 8 27.425732417455322 33.852380042441496 18.79804085245044 19.923846687652745 9 26.85086291202083 16.902664586071356 19.349455935590047 36.89701656631777 10-11 26.439188023168764 25.22537815781841 25.871694852596587 22.46373896641623 12-13 27.32606389547843 22.38948508774294 26.490109383504418 23.794341633274215 14-15 23.880714691712065 23.889323108893663 24.82812329666979 27.401838902724485 16-17 23.860243841755683 27.141489724811645 25.331090296512492 23.667176136920183 18-19 23.909488511975592 26.29887448980331 26.80209155501274 22.98954544320836 20-21 24.57296995029599 24.952156622943498 26.7549980589086 23.719875367851905 22-23 24.25227836405585 24.7067743498744 26.990177632231415 24.05076965383833 24-25 24.45004096124535 24.95097124021638 26.782357371977998 23.81663042656027 26-27 23.832748253058067 25.01074832266817 27.331178907415143 23.825324516858622 28-29 23.73920257693736 25.931759924133885 26.852948241170242 23.476089257758513 30-31 23.79068240466636 25.099153743219766 26.75417033120448 24.35599352090939 32-33 23.870764385840975 25.169307899399286 26.84152764524232 24.118400069517417 34-35 24.587416019854405 25.000478548050957 26.665624271473558 23.746481160621077 36-37 24.200041642220693 24.83038461755736 27.04406005195593 23.92551368826601 38-39 23.985399719684622 25.23957807876463 26.919937466556487 23.855084734994264 40-41 24.068782007136726 25.27855724759042 26.775656880469384 23.877003864803466 42-43 23.76329491375817 25.24708888059191 26.368249822647076 24.621366383002844 44-45 24.006138448357287 25.066709327282304 26.6084023794456 24.31874984491481 46-47 24.49597683281931 25.482748790669213 26.208458224044506 23.81281615246697 48-49 24.359993293193376 25.351789561230788 26.199098939319743 24.089118206256092 50-51 23.7802394237045 25.78541042694571 26.592494479454693 23.8418556698951 52-53 24.291786888728694 25.837710959833576 25.792880513544663 24.077621637893067 54-55 23.95732531579254 25.516392445549652 25.980249462844533 24.546032775813266 56-57 24.186178306760393 25.507158105544875 26.050865557497282 24.255798030197454 58-59 24.565002725254978 25.154637803228724 25.910651562792076 24.369707908724223 60-61 24.06987827617407 25.390778610420266 26.616220343284127 23.923122770121537 62-63 23.838295005516706 25.54278521966972 26.620408377322413 23.99851139749116 64-65 23.662321772371445 25.885150646810978 26.216758047860424 24.235769532957153 66-67 23.92891906419948 25.534834242951064 25.93304422501699 24.603202467832464 68-69 23.68305706571093 25.421747044602387 26.386665698014667 24.508530191672016 70-71 24.113188356661986 25.477228330185177 26.08554915161202 24.324034161540823 72-73 24.063394790720235 25.182617763059266 26.293931317602066 24.460056128618433 74-75 23.527929295650228 26.173632116959762 25.97359018765483 24.32484839973519 76-77 24.025244604261395 25.452138100888845 25.934446985967913 24.58817030888185 78-79 24.000935066527685 25.472555515036905 26.150317390139765 24.376192028295645 80-81 24.169415230969154 25.452966019052276 26.052779292152167 24.324839457826407 82-83 23.954555861614047 25.31790018548422 26.3420272547831 24.385516698118632 84-85 23.73294066785007 25.594914231521333 26.129624395614105 24.542520705014496 86-87 23.890674618464207 25.71285172563071 26.000171064241485 24.396302591663606 88-89 23.752223623542477 25.723942420734897 25.695091050284713 24.828742905437917 90-91 23.93167239964926 25.218853961812183 25.96091616055311 24.888557477985447 92-93 24.115066927440047 25.321459965016764 26.059607112559014 24.503865994984174 94-95 23.92459963762011 25.17071184300988 26.16752896183751 24.737159557532497 96-97 23.762890134682987 25.207210806927478 26.358514249274517 24.671384809115015 98-99 23.734139923807064 25.566745496222637 25.73152079163169 24.967593788338608 100-101 23.96633876809365 24.64436074101675 26.25150824534353 25.137792245546077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1849.0 1 1063.0 2 304.0 3 333.0 4 312.0 5 313.0 6 520.5 7 1121.0 8 1833.5 9 2152.5 10 1954.5 11 1757.0 12 1923.0 13 2106.5 14 2276.5 15 2474.5 16 2602.0 17 2801.5 18 2937.5 19 2997.0 20 3126.0 21 3378.5 22 3838.5 23 4375.5 24 5094.5 25 6330.5 26 7855.0 27 9673.5 28 11646.0 29 13565.5 30 16032.5 31 19465.5 32 23604.0 33 26258.5 34 28010.0 35 30375.5 36 33472.5 37 37990.0 38 42726.5 39 46527.0 40 51042.5 41 56097.5 42 59442.5 43 64229.5 44 72148.0 45 77986.0 46 81839.0 47 94270.0 48 114719.5 49 117567.5 50 106380.0 51 101754.5 52 103507.0 53 107412.5 54 109227.0 55 104435.5 56 97035.5 57 91714.5 58 86263.0 59 78095.0 60 64845.0 61 52889.0 62 43348.5 63 33825.5 64 25987.5 65 20381.5 66 15647.5 67 11811.0 68 8958.0 69 6816.0 70 5277.0 71 3955.0 72 2950.5 73 2459.0 74 1965.5 75 1504.5 76 1162.0 77 769.5 78 488.5 79 320.0 80 240.5 81 195.0 82 140.0 83 99.0 84 71.5 85 51.0 86 29.5 87 20.0 88 21.0 89 19.0 90 10.5 91 10.0 92 12.0 93 11.5 94 8.0 95 5.0 96 4.5 97 4.5 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.288196602423762 2 0.11344550314407258 3 0.12498301533484112 4 0.12562622158937875 5 0.13378690094382478 6 0.05885337229019215 7 0.06681304969009519 8 0.0708732891718639 9 0.06878286884461664 10-11 0.06452162740830492 12-13 0.06822006337189623 14-15 0.06428042506285331 16-17 0.06522513424920544 18-19 0.06962707705369726 20-21 0.07328531262637998 22-23 0.08190829647627493 24-25 0.0909332842352559 26-27 0.09111418599434461 28-29 0.08470222364442272 30-31 0.0805615833808368 32-33 0.07356671536274019 34-35 0.24415707418338933 36-37 0.3732204291954535 38-39 0.3434922401185429 40-41 0.32604527046421 42-43 0.3506881100911825 44-45 0.36427584221828974 46-47 0.37002449811821975 48-49 0.3802353974090044 50-51 0.3736827336909024 52-53 0.37432593994544 54-55 0.3606980074274242 56-57 0.3358943662368173 58-59 0.3197338090915596 60-61 0.2766389900375391 62-63 0.3498640020775562 64-65 0.3801750968226415 66-67 0.3848785425589479 68-69 0.39669745748607654 70-71 0.39790346921333464 72-73 0.381079605618085 74-75 0.38421523610895597 76-77 0.3677129756409751 78-79 0.34451735008671225 80-81 0.3423666291731021 82-83 0.3574015753729189 84-85 0.36552205433645635 86-87 0.3592708935501689 88-89 0.37464754307270887 90-91 0.3986069760542351 92-93 0.3931598230861197 94-95 0.3920141119452245 96-97 0.39669745748607654 98-99 0.3866473597589263 100-101 0.36495924886373593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2487538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.29105365036453 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02477397338887 31.714957948834055 2 14.745210420256813 13.356522324595307 3 5.80484770618737 7.887230066793828 4 2.9184456775368868 5.287179190279904 5 1.6867084107314974 3.819640056148067 6 1.039511313617271 2.824833760512044 7 0.697067371226399 2.2099640995693384 8 0.5018396380336849 1.8183076776050515 9 0.3845353391166192 1.5674409606952728 >10 1.9865210877126442 16.34678661410381 >50 0.13869991492570086 4.329259637855866 >100 0.06739384198359827 5.481261221943083 >500 0.002489370958233526 0.8383085135139468 >1k 0.0016892160073724738 1.6454104802207836 >5k 1.7781221130236567E-4 0.45666251269324104 >10k+ 8.890610565118284E-5 0.4162349346362783 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10337 0.4155514408222105 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5770 0.2319562555426289 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5571 0.22395637775181726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4416 0.1775249262523829 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4238 0.17036925667065186 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3965 0.15939454995260374 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3079 0.12377700360758306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.11834995083492192 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2566 0.10315420307147066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.040078181720238E-5 2 0.0 0.0 0.0 0.0 8.040078181720238E-5 3 0.0 0.0 0.0 4.020039090860119E-5 8.040078181720238E-5 4 0.0 0.0 0.0 4.020039090860119E-5 8.040078181720238E-5 5 0.0 0.0 0.0 8.040078181720238E-5 8.040078181720238E-5 6 0.0 0.0 0.0 8.040078181720238E-5 8.040078181720238E-5 7 0.0 0.0 0.0 8.040078181720238E-5 8.040078181720238E-5 8 0.0 0.0 0.0 8.040078181720238E-5 8.040078181720238E-5 9 0.0 0.0 0.0 2.0100195454300597E-4 1.6080156363440477E-4 10-11 0.0 0.0 0.0 3.2160312726880954E-4 1.6080156363440477E-4 12-13 0.0 0.0 0.0 4.221041045403126E-4 3.2160312726880954E-4 14-15 0.0 0.0 0.0 4.4220429999461316E-4 3.417033227231101E-4 16-17 0.0 0.0 0.0 5.628054727204167E-4 4.221041045403126E-4 18-19 0.0 0.0 0.0 7.035068409005209E-4 5.226050818118156E-4 20-21 0.0 0.0 0.0 8.844085999892262E-4 6.030058636290179E-4 22-23 0.0 0.0 0.0 0.0012060117272580358 6.834066454462204E-4 24-25 0.0 0.0 0.0 0.0015678152454354465 7.236070363548215E-4 26-27 0.0 0.0 0.0 0.002110520522701563 8.040078181720239E-4 28-29 0.0 0.0 0.0 0.003999938895405819 8.442082090806251E-4 30-31 0.0 0.0 0.0 0.01045210163623631 8.442082090806251E-4 32-33 0.0 0.0 0.0 0.022029814217913453 8.844085999892263E-4 34-35 0.0 0.0 0.0 0.03863257566316575 8.844085999892263E-4 36-37 0.0 0.0 0.0 0.06476282975375652 8.844085999892263E-4 38-39 0.0 0.0 0.0 0.10604863121688995 8.844085999892263E-4 40-41 0.0 0.0 0.0 0.15083186668907167 8.844085999892263E-4 42-43 0.0 0.0 0.0 0.19832862854758398 9.246089908978275E-4 44-45 0.0 0.0 0.0 0.25454887523326275 9.648093818064287E-4 46-47 0.0 0.0 0.0 0.326648276327839 0.0010050097727150298 48-49 0.0 0.0 0.0 0.4008380977496625 0.0011658113363494347 50-51 0.0 0.0 0.0 0.48600262588953413 0.0011658113363494347 52-53 0.0 0.0 0.0 0.5707450499248654 0.0011859115318037352 54-55 0.0 0.0 0.0 0.6656782730555272 0.0012060117272580358 56-57 0.0 0.0 0.0 0.7846312297540781 0.0012261119227123364 58-59 0.0 0.0 0.0 0.9098152470434622 0.001246212118166637 60-61 0.0 0.0 0.0 1.0463759749599806 0.001246212118166637 62-63 0.0 0.0 0.0 1.1838613118673966 0.0012864125090752381 64-65 0.0 0.0 0.0 1.3327434595974013 0.0012864125090752381 66-67 0.0 0.0 0.0 1.517102452304246 0.0013065127045295388 68-69 0.0 0.0 0.0 1.7233304576653703 0.00134671309543814 70-71 0.0 0.0 0.0 1.9428044918308787 0.0013668132908924407 72-73 0.0 0.0 0.0 2.1687507889326714 0.0015276148545268454 74-75 0.0 0.0 0.0 2.4093903289115586 0.0016080156363440477 76-77 0.0 0.0 0.0 2.667215536003872 0.001708516613615551 78-79 0.0 0.0 0.0 2.972698306518333 0.0017286168090698514 80-81 0.0 0.0 0.0 3.311547401486932 0.001788917395432753 82-83 0.0 0.0 0.0 3.6534919265554935 0.0020100195454300597 84-85 0.0 0.0 0.0 4.007958873392085 0.0020100195454300597 86-87 0.0 0.0 2.0100195454300596E-5 4.385983249301116 0.0020301197408843603 88-89 0.0 0.0 4.020039090860119E-5 4.808951662245963 0.002050219936338661 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9355 0.0 25.691887 1 TACATGG 9480 0.0 24.357908 2 ACATGGG 9865 0.0 23.02278 3 GAGTACT 6230 0.0 21.678175 12-13 AGTACTT 6635 0.0 20.569202 12-13 AGAGTAC 11185 0.0 20.37822 10-11 GTACTTT 6720 0.0 19.992102 14-15 TACTTTT 7065 0.0 19.183529 14-15 CATGGGG 6765 0.0 18.994194 4 GTCTTAG 675 0.0 18.296112 1 TAAGGTG 835 0.0 18.17117 5 ACTTTTT 7585 0.0 17.713787 16-17 GAGTACA 7445 0.0 17.608957 1 TAGACAG 1310 0.0 17.373579 5 GTATAGG 500 0.0 17.099829 1 CATGGGA 3965 0.0 16.861464 4 GTATCAA 19355 0.0 16.540781 1 AGTACAT 7425 0.0 16.47565 2 GTAAGGT 720 0.0 15.805129 4 TAGACTG 675 0.0 15.453905 5 >>END_MODULE