FastQCFastQC Report
Fri 27 May 2016
SRR939238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487538
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105310.42335031665847916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.22894122622448382No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53940.21684090856099483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.18311278058867844No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41300.16602761445252293No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38030.15288208662541034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30850.12401820595303469No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30180.1213247797621584No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26740.10749584528959959No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26030.10464161753508891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86950.022.7669851
TACATGG88350.021.7527982
GTGTACG2751.2332748E-920.7649211
ACATGGG92550.020.0478133
GAGTACT60900.019.63625312-13
GTATAGG4750.019.0345121
AGTACTT65200.018.95987712-13
AGAGTAC112750.018.79212610-11
TAAGGTG9150.018.670165
GTACTTT65100.018.40584614-15
TACTTTT68700.017.9248714-15
TAGACTG7250.017.6722745
TAGCGCG2807.2142757E-716.9476174
CATGGGG62650.016.890814
GGACCGA6550.016.6636
ACTTTTT75850.015.89108516-17
CGTACAC3603.5861376E-815.8177763
TAAGGTA6550.015.2140444
GTAAGGT9700.015.1654964
ACCGAGT9100.015.1224888
Produced by FastQC (version 0.11.5)