##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939238_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2487538 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.289328645431745 33.0 31.0 34.0 31.0 34.0 2 32.50015798753627 34.0 31.0 34.0 31.0 34.0 3 32.59493161511502 34.0 31.0 34.0 31.0 34.0 4 36.11567381081213 37.0 35.0 37.0 35.0 37.0 5 36.047505605944515 37.0 35.0 37.0 35.0 37.0 6 36.08470785169915 37.0 35.0 37.0 35.0 37.0 7 36.02828579905111 37.0 35.0 37.0 35.0 37.0 8 36.004084359716316 37.0 35.0 37.0 35.0 37.0 9 37.716022428602095 39.0 37.0 39.0 35.0 39.0 10-11 37.629965853787965 39.0 37.0 39.0 35.0 39.0 12-13 37.55181267582646 39.0 37.0 39.0 35.0 39.0 14-15 38.856268527355155 40.0 38.0 41.0 35.0 41.0 16-17 38.798765687197545 40.0 38.0 41.0 35.0 41.0 18-19 38.89077533689938 40.0 38.0 41.0 35.0 41.0 20-21 38.87545536992802 40.0 38.0 41.0 35.0 41.0 22-23 38.81803192554244 40.0 38.0 41.0 34.5 41.0 24-25 38.744151647130614 40.0 38.0 41.0 34.0 41.0 26-27 38.56890869606816 40.0 38.0 41.0 34.0 41.0 28-29 38.421824309819584 40.0 38.0 41.0 34.0 41.0 30-31 38.25517961936662 40.0 38.0 41.0 34.0 41.0 32-33 38.06600703185238 40.0 37.5 41.0 33.0 41.0 34-35 38.04758520271851 40.0 38.0 41.0 33.0 41.0 36-37 37.98281091585335 40.0 37.5 41.0 33.0 41.0 38-39 37.88189285952616 40.0 37.0 41.0 33.0 41.0 40-41 37.72262795583424 40.0 37.0 41.0 33.0 41.0 42-43 37.54842197385528 40.0 37.0 41.0 32.5 41.0 44-45 37.33836608727184 40.0 37.0 41.0 32.0 41.0 46-47 37.07718314252888 40.0 36.0 41.0 31.0 41.0 48-49 36.77597628659341 39.5 36.0 41.0 31.0 41.0 50-51 36.52011185356767 39.0 35.0 41.0 30.0 41.0 52-53 36.27629849272654 39.0 35.0 40.5 30.0 41.0 54-55 35.97756134780654 39.0 35.0 40.0 29.0 41.0 56-57 35.67902661185477 38.0 35.0 40.0 28.5 41.0 58-59 35.34024384753117 38.0 34.0 40.0 28.0 41.0 60-61 34.94530957919035 37.0 34.0 40.0 28.0 41.0 62-63 34.53211669530275 37.0 34.0 39.5 27.0 41.0 64-65 34.06899954895161 36.0 33.0 39.0 26.0 40.5 66-67 33.65292228701632 35.5 33.0 39.0 26.0 40.0 68-69 33.226047200082974 35.0 33.0 38.0 26.0 40.0 70-71 32.7940761105961 35.0 32.0 37.5 25.0 39.5 72-73 32.2943502772621 35.0 32.0 37.0 24.0 39.0 74-75 31.805834724936865 35.0 31.0 36.0 22.5 39.0 76-77 29.86672746305785 32.5 29.0 35.0 20.0 36.5 78-79 30.867272379356617 34.0 30.5 35.0 20.0 37.0 80-81 30.93116085060811 34.0 31.0 35.0 20.0 37.0 82-83 30.655634004385057 34.0 31.0 35.0 20.0 36.0 84-85 30.30866603846856 34.0 30.5 35.0 18.5 36.0 86-87 29.81214236727238 34.0 30.0 35.0 14.0 35.5 88-89 29.32151026436581 34.0 29.0 35.0 7.0 35.0 90-91 28.91154687888185 34.0 29.0 35.0 2.0 35.0 92-93 28.562077242639106 34.0 29.0 35.0 2.0 35.0 94-95 28.15220209701319 33.0 29.0 35.0 2.0 35.0 96-97 27.578125841695684 33.0 27.0 35.0 2.0 35.0 98-99 26.804342687428292 33.0 26.0 35.0 2.0 35.0 100-101 23.423982467805516 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28630566586380013 1101 2 0.27163611304410296 1101 3 0.2482356862235946 1101 4 0.21107897028777955 1101 5 0.2326546621633483 1101 6 0.2269751225834966 1101 7 0.22307921176025047 1101 8 0.26324505465019143 1101 9 0.2798962751119163 1101 10-11 0.280875582378485 1101 12-13 0.3556987889568006 1101 14-15 0.34033394352320556 1101 16-17 0.3200310200137153 1101 18-19 0.30331849677153855 1101 20-21 0.3271996034660276 1101 22-23 0.36815879750131586 1101 24-25 0.4142338853409129 1101 26-27 0.40053361122276243 1101 28-29 0.3650833401564668 1101 30-31 0.2624749902792729 1101 32-33 0.2646199160625713 1101 34-35 0.27301120632482423 1101 36-37 0.3631352860878323 1101 38-39 0.45852511015609565 1101 40-41 0.4597824359513609 1101 42-43 0.49373019934668605 1101 44-45 0.5615454380276574 1101 46-47 0.5534666636395968 1101 48-49 0.47350089641089 1101 50-51 0.5488195965250711 1101 52-53 0.6314430525235508 1101 54-55 0.4455763613365633 1101 56-57 0.35868655078635925 1101 58-59 0.513509818913974 1101 60-61 0.44914773138572883 1101 62-63 0.49629197792396695 1101 64-65 0.45970987673553054 1101 66-67 0.3718237092620811 1101 68-69 0.4253590009254751 1101 70-71 0.5316152745190905 1101 72-73 0.3298830519225575 1101 74-75 0.23549380916818663 1101 76-77 0.4079628233437873 1101 78-79 0.7111670785611537 1101 80-81 0.682915042591933 1101 82-83 0.5516978078353283 1101 84-85 0.5757570740187568 1101 86-87 0.6031515998881716 1101 88-89 0.7336784762217476 1101 90-91 0.7115453638958726 1101 92-93 0.6482202034596263 1101 94-95 0.8948391466286232 1101 96-97 0.8867042580124043 1101 98-99 0.8821481950816548 1101 100-101 0.7023688246924955 1102 1 0.10451708993389275 1102 2 0.1350420019876779 1102 3 0.16327090310799974 1102 4 0.10522105864201592 1102 5 0.16618816850253637 1102 6 0.14240799528778325 1102 7 0.21667560796412744 1102 8 0.21022819396765158 1102 9 0.36073047707408534 1102 10-11 0.2935705407695508 1102 12-13 0.2775356443574779 1102 14-15 0.4069657324211988 1102 16-17 0.4243432080604066 1102 18-19 0.29941317182099425 1102 20-21 0.3458656510096816 1102 22-23 0.2663152393550945 1102 24-25 0.3176821429112522 1102 26-27 0.3414946993030483 1102 28-29 0.34792275805958184 1102 30-31 0.38612643690150605 1102 32-33 0.2738284237373847 1102 34-35 0.42386229208246107 1102 36-37 0.47374157575632125 1102 38-39 0.4542276234393938 1102 40-41 0.4741919180060705 1102 42-43 0.4249674754024255 1102 44-45 0.5474677778731518 1102 46-47 0.39331405019401444 1102 48-49 0.37568050119518404 1102 50-51 0.4031244907418028 1102 52-53 0.40719775552826576 1102 54-55 0.49455158770104646 1102 56-57 0.45584017627452766 1102 58-59 0.505727018181787 1102 60-61 0.4281810014806098 1102 62-63 0.3412835038197102 1102 64-65 0.3553866058563244 1102 66-67 0.26247641042306213 1102 68-69 0.3877431095455748 1102 70-71 0.1878505329309661 1102 72-73 0.3417216957324527 1102 74-75 0.43871933022234444 1102 76-77 0.5086729013707156 1102 78-79 0.4953368721021931 1102 80-81 0.4577055391672431 1102 82-83 0.4763870243719026 1102 84-85 0.5210438768849137 1102 86-87 0.7140376126715111 1102 88-89 0.7309548464482205 1102 90-91 0.8075897588812033 1102 92-93 0.6122955333775586 1102 94-95 0.6248265822960946 1102 96-97 0.5751313558978737 1102 98-99 0.5606278350657838 1102 100-101 0.459750464777418 1103 1 0.14126685166547048 1103 2 0.22458623611531436 1103 3 0.18039494458737693 1103 4 0.16445017509912674 1103 5 0.12036521227577168 1103 6 0.18061250724813505 1103 7 0.17908226987101017 1103 8 0.18484436672314786 1103 9 0.236388438703095 1103 10-11 0.2507780348561397 1103 12-13 0.2573741693167193 1103 14-15 0.306539083899807 1103 16-17 0.2943095432183185 1103 18-19 0.22131587785753482 1103 20-21 0.2729504857413332 1103 22-23 0.2577780789783759 1103 24-25 0.23518763733080306 1103 26-27 0.2653373516412145 1103 28-29 0.24902153023698048 1103 30-31 0.31159423072027437 1103 32-33 0.40217242188710856 1103 34-35 0.35862814098284446 1103 36-37 0.463716704220154 1103 38-39 0.42335831520553313 1103 40-41 0.3829725423419674 1103 42-43 0.3391721281450515 1103 44-45 0.44512576408318694 1103 46-47 0.44469762715890937 1103 48-49 0.38918805818686764 1103 50-51 0.5085218152080841 1103 52-53 0.44796176755509975 1103 54-55 0.41109534920246205 1103 56-57 0.3217805292805096 1103 58-59 0.3355245933746076 1103 60-61 0.45554695384480226 1103 62-63 0.3781704165425168 1103 64-65 0.38717937880914377 1103 66-67 0.44059755916178744 1103 68-69 0.28200137128664693 1103 70-71 0.3598736301634773 1103 72-73 0.4048555651557635 1103 74-75 0.2993113532301983 1103 76-77 0.3883546105122875 1103 78-79 0.4645248265021422 1103 80-81 0.46770974028925494 1103 82-83 0.4271542877990093 1103 84-85 0.4497613950050443 1103 86-87 0.49279546603224134 1103 88-89 0.5790943415761483 1103 90-91 0.5722889373160385 1103 92-93 0.4821744382701887 1103 94-95 0.5304354153785908 1103 96-97 0.2941089748672816 1103 98-99 -0.04709546965299083 1103 100-101 -0.0012659721124563816 1104 1 0.13553537285991268 1104 2 0.14646414467964775 1104 3 0.2036370218844752 1104 4 0.07256181471637291 1104 5 0.1826473302502194 1104 6 0.16821554964717222 1104 7 0.22084463622644535 1104 8 0.19012966949632215 1104 9 0.18427891697337628 1104 10-11 0.170879568875975 1104 12-13 0.2248561937100888 1104 14-15 0.27685081318684723 1104 16-17 0.25825909925143264 1104 18-19 0.22704246755883872 1104 20-21 0.23946718366205033 1104 22-23 0.27043415910463864 1104 24-25 0.2502784913740115 1104 26-27 0.25584180579625837 1104 28-29 0.2878797686673664 1104 30-31 0.2665023843699714 1104 32-33 0.23396478199172321 1104 34-35 0.255223948231901 1104 36-37 0.2962520270929758 1104 38-39 0.37934007993314367 1104 40-41 0.3521633992099922 1104 42-43 0.3314056955035056 1104 44-45 0.422826378516838 1104 46-47 0.2540585958824053 1104 48-49 0.12007799829353161 1104 50-51 0.03320943766204465 1104 52-53 0.1612491215791252 1104 54-55 0.2159795281335377 1104 56-57 0.19683530535591132 1104 58-59 0.224655100588393 1104 60-61 0.25956195868682386 1104 62-63 0.20341354988613602 1104 64-65 0.128151738348798 1104 66-67 0.131387066202457 1104 68-69 0.19885925625290923 1104 70-71 0.33501923493118113 1104 72-73 0.2798246913678142 1104 74-75 0.2827151983115037 1104 76-77 0.4119555842769955 1104 78-79 0.42015294602227016 1104 80-81 0.49874088496980207 1104 82-83 0.48346717163896713 1104 84-85 0.31216190173353553 1104 86-87 0.27707913258518957 1104 88-89 0.5369982485861087 1104 90-91 0.595982622983577 1104 92-93 0.38035612607589186 1104 94-95 0.1700857973443881 1104 96-97 0.40417484377699964 1104 98-99 0.44442229662754684 1104 100-101 0.22298365903179018 1105 1 0.08092984074685461 1105 2 0.11895999435063942 1105 3 0.13370673319221282 1105 4 0.09554699439807024 1105 5 0.13181566475073936 1105 6 0.08688723107253793 1105 7 0.11452049464245562 1105 8 0.14360119308476982 1105 9 0.12460518437898571 1105 10-11 0.14494312312174884 1105 12-13 0.14690963786106437 1105 14-15 0.20962525924679198 1105 16-17 0.24169653148642567 1105 18-19 0.1338230564611962 1105 20-21 0.1872775518415608 1105 22-23 0.14589869378787768 1105 24-25 0.16181160691134977 1105 26-27 0.22425415831293805 1105 28-29 0.1645435440270262 1105 30-31 0.2645004505121733 1105 32-33 0.15121664208565022 1105 34-35 0.24909923573328285 1105 36-37 0.22588608716343117 1105 38-39 0.2713063938366531 1105 40-41 0.3486060251329306 1105 42-43 0.12220476270151437 1105 44-45 0.12683700051518088 1105 46-47 0.23509363639032443 1105 48-49 0.33430607264752155 1105 50-51 0.49152627040111696 1105 52-53 0.429313806278536 1105 54-55 0.31646583649142457 1105 56-57 0.33049776185299606 1105 58-59 0.2779190188544547 1105 60-61 0.2896817938823304 1105 62-63 0.41830549913658643 1105 64-65 0.323526298876061 1105 66-67 0.3312468259632766 1105 68-69 0.44865787198043705 1105 70-71 0.4773255736478035 1105 72-73 0.360702961418248 1105 74-75 0.3550141073170927 1105 76-77 0.5044892579313824 1105 78-79 0.5238416731243625 1105 80-81 0.46813243074073085 1105 82-83 0.5293856296717614 1105 84-85 0.49338037434136695 1105 86-87 0.46376644128790545 1105 88-89 0.7065972219351337 1105 90-91 0.7998044805535258 1105 92-93 0.6578055311657742 1105 94-95 0.7498092627891211 1105 96-97 0.6954783492497434 1105 98-99 0.7055951245035352 1105 100-101 0.591667071579824 1106 1 0.0789969853470609 1106 2 0.06872111485866839 1106 3 0.12028357348944496 1106 4 0.09448953589432563 1106 5 0.08037863485109398 1106 6 0.17605971480831073 1106 7 0.12869319657286837 1106 8 0.060132317241006206 1106 9 0.15300522417769002 1106 10-11 0.14287594710151552 1106 12-13 0.19469618415410395 1106 14-15 0.1712295484297499 1106 16-17 0.1745765436355029 1106 18-19 0.0642445386835675 1106 20-21 0.17599051766529783 1106 22-23 0.16620074499205373 1106 24-25 0.1979127828931837 1106 26-27 0.28862279903015775 1106 28-29 0.17361930644274537 1106 30-31 0.12109795820571634 1106 32-33 0.10044109105690069 1106 34-35 0.2216815954821314 1106 36-37 0.31387813789630314 1106 38-39 0.3615328449619284 1106 40-41 0.3965175533158245 1106 42-43 0.24825685361710725 1106 44-45 0.28705680106782694 1106 46-47 0.45421586553658955 1106 48-49 0.21565139097604202 1106 50-51 0.2690739546577632 1106 52-53 0.26763218891513674 1106 54-55 0.27819824956704764 1106 56-57 0.25199674157485674 1106 58-59 0.3799642008549782 1106 60-61 0.2966617531410236 1106 62-63 0.2064838880921016 1106 64-65 0.34466246455037464 1106 66-67 0.3016240234015015 1106 68-69 0.3552677731295333 1106 70-71 0.37046658149756695 1106 72-73 0.33742979743887247 1106 74-75 0.33610553436240664 1106 76-77 0.2704672150360601 1106 78-79 0.4506261125299673 1106 80-81 0.5100498030488616 1106 82-83 0.5602418428950564 1106 84-85 0.5126071623591635 1106 86-87 0.5988338110649813 1106 88-89 0.6475494757112088 1106 90-91 0.5268426366866308 1106 92-93 0.6662224330462685 1106 94-95 0.8232017355977028 1106 96-97 0.7312359995902149 1106 98-99 0.801787191644646 1106 100-101 0.9365503199918059 1107 1 0.06790092764857292 1107 2 0.07713252546914617 1107 3 0.08220473758102997 1107 4 0.038855732958623435 1107 5 0.08395889532292955 1107 6 0.06995896648444244 1107 7 0.08815998524134017 1107 8 0.04261243922932323 1107 9 0.1034750542878129 1107 10-11 0.12161883588233025 1107 12-13 0.13730194168800125 1107 14-15 0.1957401536629675 1107 16-17 0.09164230568035947 1107 18-19 0.22083618739664246 1107 20-21 0.22181820596889423 1107 22-23 0.17964101884832218 1107 24-25 0.10351717347374034 1107 26-27 0.22882959816797666 1107 28-29 0.14459276177113622 1107 30-31 0.11153012835504938 1107 32-33 0.13715929977615104 1107 34-35 0.19559181584958196 1107 36-37 0.21647626019535693 1107 38-39 0.1786856716267451 1107 40-41 0.11214056637923164 1107 42-43 0.04879418790797274 1107 44-45 0.027789547436157136 1107 46-47 -0.019190540671736755 1107 48-49 0.07233578940439855 1107 50-51 0.05699847481125886 1107 52-53 0.13336213069266734 1107 54-55 0.08500175664667609 1107 56-57 0.13762291433663876 1107 58-59 0.201435338199623 1107 60-61 0.09397789892981478 1107 62-63 0.1278184481913982 1107 64-65 0.264030099758223 1107 66-67 0.23578808917923766 1107 68-69 0.24057898823684098 1107 70-71 0.2662919333450873 1107 72-73 0.25930967120613957 1107 74-75 0.42832705874058163 1107 76-77 0.30655618323512 1107 78-79 0.5284740458459893 1107 80-81 0.47344715190503806 1107 82-83 0.5143301433906373 1107 84-85 0.3890067796864578 1107 86-87 0.3604095170500692 1107 88-89 0.5633005805512852 1107 90-91 0.4948231508115626 1107 92-93 0.4458623185278654 1107 94-95 0.5407517766670331 1107 96-97 0.6430562068482075 1107 98-99 0.8078715932107627 1107 100-101 0.6956972925267202 1108 1 0.003250653196154474 1108 2 -0.011632410985349395 1108 3 0.0480366880523988 1108 4 0.030765136495908507 1108 5 0.07962178908887552 1108 6 0.06267498980175645 1108 7 0.11769879521772708 1108 8 0.11755878657063334 1108 9 0.02273125943696641 1108 10-11 0.05752891737711252 1108 12-13 0.058831126229392794 1108 14-15 0.17389294068964034 1108 16-17 0.14805128126510425 1108 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48-49 -0.5782620246621022 2316 50-51 -0.502956160381764 2316 52-53 -0.4583101705005177 2316 54-55 -0.43704162918367473 2316 56-57 -0.23394005434619203 2316 58-59 -0.19630026301092585 2316 60-61 -0.15977661651547947 2316 62-63 -0.2616909115252284 2316 64-65 -0.31690411023432574 2316 66-67 -0.2746945669669145 2316 68-69 -0.1759415534288209 2316 70-71 -0.14012380111024925 2316 72-73 -0.236130411714214 2316 74-75 -0.2119874159268349 2316 76-77 -0.29139420130070803 2316 78-79 -0.314362011435108 2316 80-81 -0.28238001402869983 2316 82-83 -0.2983973310576147 2316 84-85 -0.5000956327127213 2316 86-87 -0.5699579123716632 2316 88-89 -0.6833678339295055 2316 90-91 -0.9886338458656354 2316 92-93 -0.8667774307501581 2316 94-95 -0.5009995990198739 2316 96-97 -0.27028967271344584 2316 98-99 -0.20643212480269924 2316 100-101 0.5865674674452634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 48.0 6 137.0 7 324.0 8 751.0 9 1289.0 10 2090.0 11 3140.0 12 4312.0 13 6049.0 14 7350.0 15 9696.0 16 12437.0 17 17000.0 18 16523.0 19 13092.0 20 12770.0 21 13616.0 22 14845.0 23 16451.0 24 19240.0 25 22769.0 26 27335.0 27 33183.0 28 40690.0 29 49303.0 30 59916.0 31 73587.0 32 91132.0 33 118170.0 34 161050.0 35 234807.0 36 380340.0 37 594221.0 38 402925.0 39 26919.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29886475416445 18.381387773531358 14.632254527276539 23.687492945027657 2 16.34596134812815 19.876641080457865 38.16271349422602 25.614684077187967 3 20.46827023345975 22.735773282659398 28.63015559963305 28.165800884247798 4 14.075443269610355 15.690534174754314 34.7344241575405 35.499598398094825 5 15.06501609221648 36.41238847406552 32.95925529579849 15.563340137919502 6 34.751428922894846 34.14697584519312 15.971655508378163 15.129939723533873 7 30.79739887390665 31.127323482093537 19.225394747738527 18.849882896261285 8 27.4890273033015 34.05097731170338 18.5629727063466 19.897022678648526 9 26.706285491920124 16.761432388168544 19.22040989926586 37.311872220645476 10-11 26.403013742905635 25.45163129166268 25.73753647180465 22.407818493627033 12-13 27.24973447641805 22.415275666140577 26.43272183178709 23.90226802565428 14-15 23.731113895726473 24.0164431671127 24.742841843361767 27.50960109379906 16-17 23.754472826561063 27.348573137674016 25.251545615092297 23.645408420672627 18-19 23.864881662109283 26.43173692220983 26.755671672151337 22.947709743529547 20-21 24.437536230602305 25.06944617529461 26.781319521551026 23.711698072552057 22-23 24.20009262170065 24.752285191221198 27.068048809706625 23.979573377371523 24-25 24.408320358651952 25.0287332409528 26.800224479073076 23.762721921322175 26-27 23.850531566022788 25.099853930147376 27.355444212552438 23.6941702912774 28-29 23.719135144870148 25.9464378031612 26.916031031485748 23.418396020482906 30-31 23.788521019578393 25.223936277556362 26.753259648696826 24.23428305416842 32-33 23.89446914981801 25.228237719383586 26.843790929022994 24.033502201775413 34-35 24.509241293139823 25.08649924350223 26.74243235892829 23.66182710442966 36-37 24.199036914265587 24.92121048360961 27.03049369402406 23.849258908100744 38-39 23.908455956107876 25.33856027106531 26.94055289701456 23.812430875812254 40-41 24.07121152266849 25.34138689777944 26.75230866502017 23.8350929145319 42-43 23.76858036904748 25.354812759570372 26.365786652627932 24.510820218754215 44-45 24.026908690510865 25.11461700763832 26.56356814317533 24.294906158675484 46-47 24.51080558312079 25.639132096110462 26.09114610918808 23.75891621158067 48-49 24.317075759244684 25.4634100061989 26.140846893595192 24.078667340961225 50-51 23.77392827767857 25.83248979513077 26.537323248931273 23.856258678259387 52-53 24.37309500397582 25.905775107757144 25.73532545030468 23.985804437962354 54-55 23.99708064761222 25.584232281074705 25.90760422554349 24.51108284576959 56-57 24.220253117741315 25.60909622285167 25.949010628179348 24.221640031227665 58-59 24.561084724994668 25.32913746176168 25.778680772792388 24.33109704045126 60-61 24.093679955762646 25.56604738791967 26.490493129521496 23.849779526796183 62-63 23.820537925520018 25.73161291826876 26.479994677388003 23.96785447882322 64-65 23.568705282090164 26.09634506085937 26.0677424827279 24.267207174322564 66-67 23.900579609236118 25.620292031719714 25.871423873725746 24.607704485318415 68-69 23.752108771504545 25.48071347179618 26.254795677553588 24.512382079145688 70-71 24.075310608320354 25.580473544524747 25.9529904668793 24.391225380275596 72-73 24.004135816216674 25.323934749941508 26.167539953158503 24.50438948068331 74-75 23.462797352241456 26.222634588898742 25.819082964762753 24.495485094097056 76-77 23.8904441890148 25.553929178381786 25.861022320767468 24.694604311835942 78-79 23.897886183045244 25.55231317069327 26.06732037862336 24.482480267638124 80-81 24.046636998444228 25.51758585895132 25.946669561127393 24.48910758147706 82-83 23.812118649041743 25.440596284358268 26.290311946993373 24.456973119606616 84-85 23.591900907644426 25.644552967633054 26.07144091869149 24.692105206031023 86-87 23.763751078515963 25.746047319485406 25.90305590254079 24.587145699457842 88-89 23.596864951687404 25.777987403240154 25.64451928378296 24.980628361289483 90-91 23.719917708058887 25.256597456599632 25.867149917308623 25.15633491803285 92-93 23.752059265024293 25.433098911453815 25.95918132707922 24.855660496442667 94-95 23.584267573855673 25.32787947920383 26.044129987990132 25.043722958950365 96-97 23.37664791452432 25.266247188987666 26.298392225566005 25.058712670922013 98-99 23.238137467648738 25.679165504205365 25.679326305768996 25.403370722376906 100-101 23.39326819251516 24.800002733630976 26.257590851019526 25.54913822283434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 464.0 1 305.0 2 202.0 3 274.5 4 256.0 5 258.5 6 467.5 7 972.0 8 1569.5 9 1847.5 10 1656.0 11 1491.0 12 1583.0 13 1739.5 14 1908.0 15 2009.0 16 2087.0 17 2161.0 18 2323.5 19 2527.0 20 2726.0 21 3046.5 22 3572.0 23 4206.5 24 5088.5 25 6330.0 26 7689.0 27 9534.0 28 11559.0 29 13627.5 30 16108.0 31 19599.0 32 23864.0 33 26366.0 34 28084.5 35 30355.5 36 33593.5 37 38232.5 38 42931.0 39 46847.0 40 51164.0 41 56194.0 42 59826.0 43 64669.0 44 72666.0 45 78783.0 46 82407.5 47 95193.0 48 116231.0 49 118576.0 50 107356.5 51 102828.5 52 104008.5 53 107802.5 54 110274.0 55 105559.0 56 97706.5 57 92115.5 58 86530.0 59 77916.0 60 64637.0 61 52950.0 62 43139.5 63 33395.5 64 25242.5 65 19577.0 66 15025.0 67 11446.0 68 8685.5 69 6455.5 70 4939.5 71 3698.0 72 2774.5 73 2275.0 74 1899.5 75 1494.5 76 1025.5 77 629.5 78 426.5 79 304.5 80 191.0 81 107.0 82 71.0 83 43.0 84 23.5 85 17.0 86 10.0 87 5.5 88 6.0 89 3.5 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2821263433965632 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002452223845424673 16-17 6.834066454462204E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.020039090860119E-5 26-27 0.0011859115318037352 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003557734595411206 36-37 0.021386607963375836 38-39 0.006713465281736399 40-41 0.017708272195238826 42-43 0.005406952577206861 44-45 5.829056681747174E-4 46-47 1.8090175908870538E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.427052772661161E-4 60-61 0.001909518568158557 62-63 0.0015075146590725447 64-65 0.0 66-67 0.0 68-69 4.6230449544891374E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23639839873802934 78-79 0.0 80-81 0.0011256109454408335 82-83 0.0 84-85 0.0 86-87 0.10620943278052436 88-89 0.3635522351819349 90-91 0.36361253576829783 92-93 0.0 94-95 2.0100195454300596E-5 96-97 0.0 98-99 0.0 100-101 1.6080156363440477E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2487538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.568260643590556 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19191273088491 29.710030276441746 2 15.465141643117358 13.475786439947669 3 6.26889366853632 8.19374379893235 4 3.076891040436028 5.362191632865816 5 1.8075714832446466 3.9376372756962175 6 1.126998035433804 2.946080649155669 7 0.7778800148524512 2.3723615465572183 8 0.5223112044102298 1.8204952552650078 9 0.39072361192849975 1.5320833347695404 >10 2.142468204361673 16.949549025940865 >50 0.1523711975473724 4.53493510681629 >100 0.07165882676913332 5.6237752764322355 >500 0.0027741098987276204 0.811394567490439 >1k 0.002126817589023317 1.8589154289255327 >5k 1.8494065991507105E-4 0.446750464692036 >10k+ 9.247032995753552E-5 0.42426992007140696 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10531 0.42335031665847916 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5695 0.22894122622448382 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5394 0.21684090856099483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.18311278058867844 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4130 0.16602761445252293 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3803 0.15288208662541034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 0.12401820595303469 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3018 0.1213247797621584 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2674 0.10749584528959959 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2603 0.10464161753508891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.040078181720238E-5 2 0.0 0.0 0.0 0.0 8.040078181720238E-5 3 0.0 0.0 0.0 4.020039090860119E-5 8.040078181720238E-5 4 0.0 0.0 0.0 4.020039090860119E-5 8.040078181720238E-5 5 0.0 0.0 0.0 8.040078181720238E-5 8.040078181720238E-5 6 0.0 0.0 0.0 2.4120234545160717E-4 1.6080156363440477E-4 7 0.0 0.0 0.0 2.4120234545160717E-4 1.6080156363440477E-4 8 0.0 0.0 0.0 2.4120234545160717E-4 1.6080156363440477E-4 9 0.0 0.0 0.0 3.6180351817741076E-4 1.6080156363440477E-4 10-11 0.0 0.0 0.0 5.226050818118156E-4 2.0100195454300597E-4 12-13 0.0 0.0 0.0 6.030058636290179E-4 3.417033227231101E-4 14-15 0.0 0.0 0.0 6.030058636290179E-4 4.221041045403126E-4 16-17 0.0 0.0 0.0 7.236070363548215E-4 6.030058636290179E-4 18-19 0.0 0.0 0.0 9.246089908978275E-4 6.432062545376191E-4 20-21 0.0 0.0 0.0 0.0010452101636236311 6.834066454462204E-4 22-23 0.0 0.0 0.0 0.0013065127045295388 8.442082090806251E-4 24-25 0.0 0.0 0.0 0.001648216027252649 9.246089908978275E-4 26-27 0.0 0.0 0.0 0.0022713220863359676 9.648093818064287E-4 28-29 0.0 0.0 0.0 0.004160740459040224 9.648093818064287E-4 30-31 0.0 0.0 0.0 0.010612903199870715 0.0010050097727150298 32-33 0.0 0.0 0.0 0.02227101656336506 0.0010452101636236311 34-35 0.0 0.0 0.0 0.03903457957225176 0.0010452101636236311 36-37 0.0 0.0 0.0 0.06514473346738824 0.0010452101636236311 38-39 2.0100195454300596E-5 0.0 0.0 0.10701344059869639 0.0010452101636236311 40-41 4.020039090860119E-5 0.0 0.0 0.15181677626633241 0.0010452101636236311 42-43 4.020039090860119E-5 0.0 0.0 0.19941403910211625 0.0010653103590779318 44-45 4.020039090860119E-5 0.0 0.0 0.2556141855923407 0.0011055107499865328 46-47 4.020039090860119E-5 0.0 0.0 0.32781408766418846 0.001145711140895134 48-49 4.020039090860119E-5 0.0 0.0 0.4022250112360093 0.0012060117272580358 50-51 4.020039090860119E-5 0.0 0.0 0.4877513428940583 0.0012060117272580358 52-53 4.020039090860119E-5 0.0 0.0 0.5729761716202928 0.0012864125090752381 54-55 4.020039090860119E-5 0.0 0.0 0.6679696953373175 0.0013266128999838394 56-57 4.020039090860119E-5 0.0 0.0 0.7879075616131291 0.0013266128999838394 58-59 4.020039090860119E-5 0.0 0.0 0.9133126810525105 0.00134671309543814 60-61 4.020039090860119E-5 0.0 0.0 1.0509990199144696 0.0014070136818010418 62-63 4.020039090860119E-5 0.0 0.0 1.1894491662036921 0.0014874144636182443 64-65 4.020039090860119E-5 0.0 0.0 1.3383514141291508 0.0014874144636182443 66-67 4.020039090860119E-5 0.0 0.0 1.522710406835996 0.0014874144636182443 68-69 4.020039090860119E-5 0.0 0.0 1.728596708874397 0.0014874144636182443 70-71 4.020039090860119E-5 0.0 0.0 1.9486938490989887 0.001507514659072545 72-73 4.020039090860119E-5 0.0 0.0 2.1756451559734966 0.0016080156363440477 74-75 4.020039090860119E-5 0.0 0.0 2.416264595756929 0.0016884164181612503 76-77 4.020039090860119E-5 0.0 0.0 2.6749340110583235 0.0017286168090698514 78-79 4.020039090860119E-5 2.0100195454300596E-5 0.0 2.9812408895864104 0.0017286168090698514 80-81 4.020039090860119E-5 4.020039090860119E-5 0.0 3.32008998455501 0.001788917395432753 82-83 4.020039090860119E-5 4.020039090860119E-5 0.0 3.662416413337203 0.001849217981795655 84-85 4.020039090860119E-5 4.020039090860119E-5 0.0 4.015858250205625 0.001849217981795655 86-87 4.020039090860119E-5 4.020039090860119E-5 0.0 4.393701724355568 0.001849217981795655 88-89 4.020039090860119E-5 4.020039090860119E-5 0.0 4.815846029286789 0.001849217981795655 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8695 0.0 22.766985 1 TACATGG 8835 0.0 21.752798 2 GTGTACG 275 1.2332748E-9 20.764921 1 ACATGGG 9255 0.0 20.047813 3 GAGTACT 6090 0.0 19.636253 12-13 GTATAGG 475 0.0 19.034512 1 AGTACTT 6520 0.0 18.959877 12-13 AGAGTAC 11275 0.0 18.792126 10-11 TAAGGTG 915 0.0 18.67016 5 GTACTTT 6510 0.0 18.405846 14-15 TACTTTT 6870 0.0 17.92487 14-15 TAGACTG 725 0.0 17.672274 5 TAGCGCG 280 7.2142757E-7 16.947617 4 CATGGGG 6265 0.0 16.89081 4 GGACCGA 655 0.0 16.663 6 ACTTTTT 7585 0.0 15.891085 16-17 CGTACAC 360 3.5861376E-8 15.817776 3 TAAGGTA 655 0.0 15.214044 4 GTAAGGT 970 0.0 15.165496 4 ACCGAGT 910 0.0 15.122488 8 >>END_MODULE