FastQCFastQC Report
Fri 27 May 2016
SRR939237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903200
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.43778898696931484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63960.3360655737704918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.2385981504833964No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19210.10093526691887349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.042.0619351
GTATCAA82400.040.601231
ATCAACG106350.031.3025363
TCAACGC106200.031.3020954
TATCAAC107600.031.0270352
CAACGCA110150.030.1365435
AACGCAG114250.029.0382376
ACGCAGA128800.025.6843157
CGCAGAG129350.025.5018228
GCAGAGT137500.023.5409289
GTACATG86250.023.383371
TACATGG86000.022.8838732
GAGTACT72700.022.58841712-13
ACATGGG87150.021.6024513
GTACTTT76150.021.25721414-15
AGTACTT75850.020.90054112-13
AGAGTAC121350.020.73605210-11
ATCACGC1404.907423E-420.3237153
CATGGGG48000.019.9567584
CAGAGTA131350.019.60833510-11
Produced by FastQC (version 0.11.5)