##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1903200 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.885868537200505 33.0 31.0 34.0 30.0 34.0 2 31.96158732660782 34.0 31.0 34.0 30.0 34.0 3 32.006212168978564 34.0 31.0 34.0 30.0 34.0 4 35.49442990752417 37.0 35.0 37.0 33.0 37.0 5 35.523246637242536 37.0 35.0 37.0 33.0 37.0 6 35.60803804119378 37.0 35.0 37.0 33.0 37.0 7 35.5360429802438 37.0 35.0 37.0 33.0 37.0 8 35.518120533837745 37.0 35.0 37.0 33.0 37.0 9 37.144279634300126 39.0 37.0 39.0 34.0 39.0 10-11 37.039763818831446 39.0 37.0 39.0 33.0 39.0 12-13 36.93003152585119 39.0 37.0 39.0 33.0 39.0 14-15 38.15799810844893 40.0 38.0 41.0 33.0 41.0 16-17 38.073823823034886 40.0 38.0 41.0 33.0 41.0 18-19 37.99200136612022 40.0 38.0 41.0 33.0 41.0 20-21 37.955702501050865 40.0 38.0 41.0 33.0 41.0 22-23 37.96413172551492 40.0 38.0 41.0 33.0 41.0 24-25 37.87357923497268 40.0 38.0 41.0 33.0 41.0 26-27 37.77467213114754 40.0 38.0 41.0 32.5 41.0 28-29 37.608848518284994 40.0 38.0 41.0 32.0 41.0 30-31 37.39634510298445 40.0 37.0 41.0 32.0 41.0 32-33 37.14392601933585 40.0 36.5 41.0 31.0 41.0 34-35 36.83947667087011 40.0 36.0 41.0 30.5 41.0 36-37 36.58855348886087 40.0 36.0 41.0 30.0 41.0 38-39 35.96994903320723 38.5 35.0 40.5 28.5 41.0 40-41 35.917084384195036 39.0 35.0 40.0 29.0 41.0 42-43 36.06560503362758 39.0 35.0 41.0 29.0 41.0 44-45 35.94531394493485 39.0 35.0 41.0 28.5 41.0 46-47 35.627376523749476 39.0 35.0 41.0 26.5 41.0 48-49 35.331379781420765 39.0 35.0 41.0 26.0 41.0 50-51 34.17559084699454 38.0 34.0 40.0 23.5 40.5 52-53 34.25441650903741 38.0 33.5 39.5 24.0 40.5 54-55 34.70355270071458 38.0 34.5 40.0 24.0 41.0 56-57 34.57744798234552 38.0 34.0 40.0 23.5 41.0 58-59 34.33443621269441 38.0 34.0 40.0 23.0 41.0 60-61 34.00778399537621 37.5 34.0 40.0 22.5 41.0 62-63 33.59932692307692 37.0 33.5 40.0 21.5 41.0 64-65 33.19044530264817 36.0 33.0 39.5 20.0 41.0 66-67 32.812392023959646 36.0 33.0 39.0 18.5 41.0 68-69 32.38938051702396 35.0 32.0 39.0 17.5 40.5 70-71 31.964513451029845 35.0 32.0 38.0 13.5 40.0 72-73 31.528758144178227 35.0 31.5 37.0 9.5 39.5 74-75 31.078984604876 35.0 31.0 37.0 7.5 39.0 76-77 30.624752259352668 35.0 31.0 36.0 4.5 39.0 78-79 30.241540300546447 35.0 30.0 36.0 2.0 38.5 80-81 29.858637820512822 34.0 30.0 35.0 2.0 37.0 82-83 29.43825136612022 34.0 29.5 35.0 2.0 37.0 84-85 29.035985445565366 34.0 29.0 35.0 2.0 36.0 86-87 28.623435266918875 34.0 29.0 35.0 2.0 36.0 88-89 28.190961801176964 34.0 28.5 35.0 2.0 35.5 90-91 27.78628835645229 34.0 27.0 35.0 2.0 35.0 92-93 27.34389029003783 34.0 26.5 35.0 2.0 35.0 94-95 26.848795187053383 33.0 25.5 35.0 2.0 35.0 96-97 26.232172656578392 33.0 24.5 35.0 2.0 35.0 98-99 25.425380149222363 33.0 21.0 35.0 2.0 35.0 100-101 22.676949085750316 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19754098406873055 1101 2 0.247503007533723 1101 3 0.34458416063833397 1101 4 0.2637398343354178 1101 5 0.12424007173614626 1101 6 0.21707147601243548 1101 7 0.1908211149675907 1101 8 0.1871529824263547 1101 9 0.26765870565110106 1101 10-11 0.3607343913345957 1101 12-13 0.43240933751412314 1101 14-15 0.4603646145774718 1101 16-17 0.4960586495447572 1101 18-19 0.48098850811016547 1101 20-21 0.4387290184514967 1101 22-23 0.5419758861441082 1101 24-25 0.7101707939372446 1101 26-27 0.547328668753444 1101 28-29 0.6315238997329544 1101 30-31 0.7150293647013086 1101 32-33 0.5876426396829544 1101 34-35 0.405203755222864 1101 36-37 0.549389819278467 1101 38-39 0.8229454802346368 1101 40-41 0.7131293390458708 1101 42-43 0.6151134460796541 1101 44-45 0.614949599377546 1101 46-47 0.6449008142612911 1101 48-49 0.5589255683746828 1101 50-51 0.43846998874948184 1101 52-53 0.574271729559733 1101 54-55 0.5396918324800595 1101 56-57 0.6139518131944826 1101 58-59 0.5764256642577834 1101 60-61 0.46773476860296626 1101 62-63 0.5776523758129031 1101 64-65 0.523360104908484 1101 66-67 0.47436716901489007 1101 68-69 0.522059591181808 1101 70-71 0.5679933360010239 1101 72-73 0.49674492464254527 1101 74-75 0.509702791778583 1101 76-77 0.48743505829346034 1101 78-79 0.4737407760287162 1101 80-81 0.4824383634948468 1101 82-83 0.591956201037739 1101 84-85 0.4729233068973855 1101 86-87 0.4916994985872698 1101 88-89 0.3985327381660859 1101 90-91 0.5312919618185568 1101 92-93 0.49747909203503937 1101 94-95 0.5209825798575984 1101 96-97 0.47984032045026126 1101 98-99 0.46834035305773014 1101 100-101 0.22723093398674976 1102 1 0.30081480858622456 1102 2 0.3546533457343486 1102 3 0.4135924678321601 1102 4 0.3505497446346979 1102 5 0.36607340816291867 1102 6 0.355725779552472 1102 7 0.36849708933275593 1102 8 0.3643576501518595 1102 9 0.41784444421710987 1102 10-11 0.510072308252056 1102 12-13 0.5474698052942486 1102 14-15 0.6987536341270868 1102 16-17 0.6569618082935662 1102 18-19 0.5733447231267519 1102 20-21 0.711191320549112 1102 22-23 0.6886699120175805 1102 24-25 0.7519546796251646 1102 26-27 0.697052712418575 1102 28-29 0.6603080744359104 1102 30-31 0.6478297215951159 1102 32-33 0.600676820351822 1102 34-35 0.49042441423019056 1102 36-37 0.5308007164713118 1102 38-39 0.967668768218239 1102 40-41 0.7691820166774264 1102 42-43 0.7349062037339209 1102 44-45 0.5950559516099858 1102 46-47 0.6817502321213595 1102 48-49 0.5356976415670758 1102 50-51 0.575829027628636 1102 52-53 0.5740652428955215 1102 54-55 0.590017902432308 1102 56-57 0.58387038519502 1102 58-59 0.6047580872379257 1102 60-61 0.524685646651875 1102 62-63 0.5860788877725582 1102 64-65 0.4976970937959848 1102 66-67 0.5570463517121738 1102 68-69 0.4789970127964196 1102 70-71 0.5181326067816059 1102 72-73 0.31969172466270024 1102 74-75 0.37740781348123065 1102 76-77 0.3777472210540047 1102 78-79 0.3192161660001567 1102 80-81 0.24502211819796926 1102 82-83 0.17296459838304656 1102 84-85 0.31175599105166896 1102 86-87 0.17583555573270004 1102 88-89 0.19387230432534253 1102 90-91 0.27777539118089933 1102 92-93 0.09079846956557347 1102 94-95 0.04490818632516991 1102 96-97 -0.006415555967965503 1102 98-99 0.16936456668492283 1102 100-101 -0.1377327535270041 1103 1 0.3881277479669052 1103 2 0.47842143497735634 1103 3 0.4993556291031709 1103 4 0.4057969821083347 1103 5 0.37367031452815525 1103 6 0.3299634736037049 1103 7 0.4087380270291092 1103 8 0.32898546983677335 1103 9 0.43689115468065864 1103 10-11 0.5242533865502281 1103 12-13 0.4672035697977108 1103 14-15 0.6519152012090217 1103 16-17 0.6501740038728698 1103 18-19 0.7490973244577646 1103 20-21 0.7369055968642471 1103 22-23 0.7416339325584786 1103 24-25 0.7026772624351594 1103 26-27 0.5915779921254014 1103 28-29 0.5623460441640873 1103 30-31 0.48836442958480575 1103 32-33 0.622523559764744 1103 34-35 0.6877485714910563 1103 36-37 0.6726294283231127 1103 38-39 0.9906190378092319 1103 40-41 0.8401132323206113 1103 42-43 0.6875963566721097 1103 44-45 0.6123701410770153 1103 46-47 0.6066195487517874 1103 48-49 0.5865462199228162 1103 50-51 0.6280191460249682 1103 52-53 0.7011957354624414 1103 54-55 0.7897310405109295 1103 56-57 0.823623789213066 1103 58-59 0.8466042345046745 1103 60-61 0.8334201315998513 1103 62-63 0.8206223924225426 1103 64-65 0.7898263198280446 1103 66-67 0.612181081413901 1103 68-69 0.681733221547681 1103 70-71 0.833830237210023 1103 72-73 0.8510688921644025 1103 74-75 0.7642041053303394 1103 76-77 0.7054585103206996 1103 78-79 0.7207399554724567 1103 80-81 0.7100585187387942 1103 82-83 0.5829886460968652 1103 84-85 0.6050267433075831 1103 86-87 0.5692035186511895 1103 88-89 0.48135565021436477 1103 90-91 0.3758686805199609 1103 92-93 0.4148894429551824 1103 94-95 0.294840418485947 1103 96-97 0.2567314048418261 1103 98-99 0.6075320058830265 1103 100-101 0.30198923384712373 1104 1 0.3854075866970277 1104 2 0.3305697981895719 1104 3 0.35244631805009874 1104 4 0.3438801907702569 1104 5 0.3255010825886089 1104 6 0.31257593144786 1104 7 0.31808204406894447 1104 8 0.3328653922446634 1104 9 0.4190630364160839 1104 10-11 0.4386738546385729 1104 12-13 0.48971494870584564 1104 14-15 0.5153313776423261 1104 16-17 0.464161027480138 1104 18-19 0.3557796595745373 1104 20-21 0.5492284155370655 1104 22-23 0.6039291756043426 1104 24-25 0.5029170436051302 1104 26-27 0.4746110575208391 1104 28-29 0.3943996993609815 1104 30-31 0.4601236594958067 1104 32-33 0.36516593874894454 1104 34-35 0.2143040587238616 1104 36-37 0.20242917221845147 1104 38-39 0.629206848748197 1104 40-41 0.41743941078247104 1104 42-43 0.26022421883201474 1104 44-45 0.13470845915395557 1104 46-47 0.22059595415787925 1104 48-49 0.25974461229546364 1104 50-51 0.16326190663048834 1104 52-53 0.28675308008804734 1104 54-55 0.23824100577196816 1104 56-57 0.22691971055596838 1104 58-59 0.2543563137703373 1104 60-61 0.2961551583513824 1104 62-63 0.2797321751139279 1104 64-65 0.2821777284037523 1104 66-67 0.2957754851464358 1104 68-69 0.18836817096066483 1104 70-71 0.1484600581903237 1104 72-73 0.07636731116178908 1104 74-75 0.07464766087462138 1104 76-77 0.0554775279944586 1104 78-79 0.14664884179748228 1104 80-81 0.07231604339837716 1104 82-83 0.08354158496138453 1104 84-85 0.16997732905193175 1104 86-87 0.12904136128627997 1104 88-89 0.0638864905419787 1104 90-91 0.03983367532179827 1104 92-93 0.11505315343011802 1104 94-95 0.2887679167743542 1104 96-97 0.2757812501647443 1104 98-99 0.19024288018887958 1104 100-101 -0.03185493533410266 1105 1 0.04300797280675894 1105 2 0.1776627258012553 1105 3 0.29880010923150735 1105 4 0.1809414627066559 1105 5 0.09348808646421958 1105 6 0.1639808410410879 1105 7 0.21671614462198363 1105 8 0.28835481535415397 1105 9 0.40027133487213007 1105 10-11 0.35459580047676553 1105 12-13 0.44721127751844136 1105 14-15 0.527753689994455 1105 16-17 0.3759356504425355 1105 18-19 0.4226943077937406 1105 20-21 0.5467686594926136 1105 22-23 0.5081036241399843 1105 24-25 0.4000226419542443 1105 26-27 0.35749655034297945 1105 28-29 0.38782664091713315 1105 30-31 0.48176022887701464 1105 32-33 0.4511712150980287 1105 34-35 0.45083391569875175 1105 36-37 0.30567330436667817 1105 38-39 0.611407637902289 1105 40-41 0.5983095003425021 1105 42-43 0.43085217936947373 1105 44-45 0.29526620747621735 1105 46-47 0.209094009093846 1105 48-49 0.17030251181984823 1105 50-51 0.15569832311611975 1105 52-53 0.04724106375338266 1105 54-55 0.17147408202139047 1105 56-57 0.21521046738909178 1105 58-59 0.24670684745333205 1105 60-61 0.2502234327602366 1105 62-63 0.19683561429680907 1105 64-65 0.1361331153118286 1105 66-67 0.24299112839653247 1105 68-69 0.21763334076668883 1105 70-71 0.233484486404393 1105 72-73 0.26356354610810584 1105 74-75 0.21766278834405384 1105 76-77 0.4148329473202672 1105 78-79 0.36274343021322153 1105 80-81 0.23062466022560102 1105 82-83 0.14440633406263714 1105 84-85 0.3259530484111295 1105 86-87 0.22880754233206702 1105 88-89 0.1594790395435588 1105 90-91 0.24552329003461182 1105 92-93 0.22563920678873828 1105 94-95 0.1754826032174499 1105 96-97 0.24566314921495547 1105 98-99 0.2770536102168961 1105 100-101 -0.04990816573709722 1106 1 0.14841507261391484 1106 2 0.08535547149889311 1106 3 0.1390512525483203 1106 4 0.11408146883068326 1106 5 0.1614903803607035 1106 6 0.23462564790465024 1106 7 0.25494078030322953 1106 8 0.2665142829935334 1106 9 0.24606112261697888 1106 10-11 0.38023479997068677 1106 12-13 0.36122444011490984 1106 14-15 0.4128646575272157 1106 16-17 0.4258671182515812 1106 18-19 0.4229480715444751 1106 20-21 0.42249094927542075 1106 22-23 0.5104216332609539 1106 24-25 0.4369576871494658 1106 26-27 0.4720307202784042 1106 28-29 0.4575762296949293 1106 30-31 0.4523159351769266 1106 32-33 0.36850509498454187 1106 34-35 0.26456391455412387 1106 36-37 0.1294996724394437 1106 38-39 0.6545711523980557 1106 40-41 0.5091742202959821 1106 42-43 0.4313646456045106 1106 44-45 0.2842795589066185 1106 46-47 0.20700245543217477 1106 48-49 0.19179181307038817 1106 50-51 0.15323709072198 1106 52-53 0.21752598219745778 1106 54-55 0.11249746895838086 1106 56-57 0.27281062635821485 1106 58-59 0.32447359862076297 1106 60-61 0.3599379797708835 1106 62-63 0.23263896322298905 1106 64-65 0.3150314755941821 1106 66-67 0.19349949838785818 1106 68-69 0.21349148639425408 1106 70-71 0.10180797229035932 1106 72-73 -0.06908935563027185 1106 74-75 0.05546911329296833 1106 76-77 0.12710392807721504 1106 78-79 0.3038533902443419 1106 80-81 0.38536796519123584 1106 82-83 0.227317212000969 1106 84-85 0.12233777924143396 1106 86-87 0.2557670720251828 1106 88-89 0.3391399983899639 1106 90-91 0.29702629191307395 1106 92-93 0.18580817538943606 1106 94-95 0.11884448049399055 1106 96-97 0.3834715368114132 1106 98-99 0.45625889826417065 1106 100-101 0.5149561440109771 1107 1 0.16680225397665538 1107 2 0.002702311693767001 1107 3 0.10349361146464275 1107 4 0.14264958509274805 1107 5 0.07082805649965707 1107 6 0.16961219164783614 1107 7 0.20093688833971868 1107 8 0.09205025605341888 1107 9 0.16871566255100845 1107 10-11 0.2531917261475911 1107 12-13 0.28230646117567915 1107 14-15 0.19474480713319053 1107 16-17 0.20655773475516526 1107 18-19 0.2798307743889268 1107 20-21 0.24685684905045946 1107 22-23 0.1428284400567108 1107 24-25 0.19697562882522135 1107 26-27 0.21639993236281185 1107 28-29 0.2693634966256653 1107 30-31 0.14534504209551358 1107 32-33 0.1531319771353381 1107 34-35 0.2055776192341412 1107 36-37 0.12714213624557402 1107 38-39 0.6995864028224403 1107 40-41 0.5589343538924609 1107 42-43 0.2874274138670714 1107 44-45 0.3846709634637975 1107 46-47 0.4618616132775202 1107 48-49 0.16093240680184095 1107 50-51 0.1905704056535953 1107 52-53 0.2740160383687069 1107 54-55 0.43025945192758286 1107 56-57 0.272728094649743 1107 58-59 0.31219348765839783 1107 60-61 0.2837725299178686 1107 62-63 0.39178452541849396 1107 64-65 0.18247395872210603 1107 66-67 0.23435352215367544 1107 68-69 0.4357532416421961 1107 70-71 0.39524131896494197 1107 72-73 0.5235580076334578 1107 74-75 0.4335279619894372 1107 76-77 0.5246190059854925 1107 78-79 0.44581344931386013 1107 80-81 0.3628401210905956 1107 82-83 0.4475151224858074 1107 84-85 0.5300444190415909 1107 86-87 0.6620967572177818 1107 88-89 0.4809708773940642 1107 90-91 0.5731209411533129 1107 92-93 0.6221562162964567 1107 94-95 0.7109011485867889 1107 96-97 0.8788718432000309 1107 98-99 0.8048103149664918 1107 100-101 0.4725046210387305 1108 1 0.06151183969295104 1108 2 0.017222020173207397 1108 3 0.0954131013707098 1108 4 -0.03230110180425072 1108 5 0.12945962627216545 1108 6 0.08257681092118219 1108 7 0.21895055252787898 1108 8 0.1402535963372955 1108 9 0.2709023349390236 1108 10-11 0.21269946672915552 1108 12-13 0.31195211753274776 1108 14-15 0.333421030238938 1108 16-17 0.30001461344694036 1108 18-19 0.36025169574944016 1108 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-1.1764378766483148 2316 54-55 -1.1221475585376766 2316 56-57 -1.2648031120076766 2316 58-59 -1.0983486886119351 2316 60-61 -0.8239995038735799 2316 62-63 -1.1251023740897708 2316 64-65 -1.2301461396261502 2316 66-67 -1.455884783230264 2316 68-69 -1.3394150851766184 2316 70-71 -1.2247183330335432 2316 72-73 -1.0676789107879259 2316 74-75 -1.0376711301805308 2316 76-77 -1.0258790396581823 2316 78-79 -1.0991682679979569 2316 80-81 -0.7855774807250633 2316 82-83 -0.9259926318957312 2316 84-85 -0.8725025485539177 2316 86-87 -0.7991868135803522 2316 88-89 -0.9803669110311048 2316 90-91 -0.9661137076929869 2316 92-93 -0.9909455363198596 2316 94-95 -0.7553956613993371 2316 96-97 -0.7324646843500879 2316 98-99 -0.932570847560303 2316 100-101 -0.09706037706995829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8700.0 3 2216.0 4 2158.0 5 2460.0 6 2878.0 7 3398.0 8 4050.0 9 4913.0 10 5632.0 11 6883.0 12 8358.0 13 10715.0 14 11058.0 15 12059.0 16 15114.0 17 18518.0 18 16593.0 19 12321.0 20 12097.0 21 12454.0 22 13535.0 23 14848.0 24 16808.0 25 18765.0 26 21573.0 27 25056.0 28 29570.0 29 35480.0 30 42551.0 31 51782.0 32 65248.0 33 84350.0 34 117332.0 35 173389.0 36 267780.0 37 391131.0 38 316469.0 39 44948.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60814616214758 18.488055190206396 14.945345170646164 22.958453476999864 2 15.506950088218327 19.673265846664336 40.18881669537096 24.63096736974638 3 19.416273780429712 23.907678006810087 29.36548499891306 27.31056321384714 4 13.11482832162733 16.307536959324604 36.107131895340395 34.47050282370767 5 14.365446979601442 36.72566860941142 33.34188077841073 15.567003632576402 6 32.67251477676136 35.201488380313364 17.114795592298673 15.011201250626602 7 29.15842469644999 32.60569011594874 19.832179457503 18.40370573009827 8 27.096816964264576 34.20207333762319 19.271264390797256 19.42984530731498 9 26.892913161271924 16.903978536434444 19.837276370767416 36.365831931526216 10-11 25.599947179342703 26.175160980686 26.388732154285773 21.836159685685523 12-13 26.533139431355885 23.477762951939297 27.13707097017534 22.852026646529477 14-15 23.293960613600966 24.832671860067034 25.652114780935154 26.22125274539685 16-17 22.703553549143862 28.06994796508914 26.32748198108754 22.89901650467946 18-19 22.70345964796343 26.101942651733822 29.054355523893005 22.140242176409743 20-21 23.393520956202472 25.302826131632827 29.13861941334369 22.16503349882101 22-23 23.049860950588513 25.23654715364486 29.09961558784954 22.61397630791708 24-25 22.817081060706958 25.53399371762807 29.122168286919198 22.52675693474577 26-27 22.396020974098175 25.83598360493957 28.99846707500806 22.769528345954196 28-29 22.41492194819002 26.086093830591256 29.08366754034168 22.415316680877044 30-31 22.503280543790098 25.716358408256735 28.903360324089043 22.877000723864125 32-33 22.24527738341318 25.758927807813812 28.890295297580437 23.10549951119257 34-35 23.069387376338767 25.89149710562988 28.528882803186523 22.51023271484483 36-37 23.136083539226185 25.467412622997937 28.582370645138553 22.81413319263732 38-39 22.68386682113843 25.793450921487732 28.57072373151147 22.951958525862366 40-41 22.858481534078543 25.723495148294838 28.715404180373 22.702619137253617 42-43 22.765230819408767 25.682330470927432 28.396716686202662 23.155722023461138 44-45 22.751916576569776 25.973923844664075 28.445688134840015 22.828471443926134 46-47 23.110460858570864 26.31876044553911 27.735725122628285 22.835053573261742 48-49 23.036780353828096 26.308881281063684 27.555137010796855 23.099201354311365 50-51 23.00501971465218 26.57915155737835 27.534058579172672 22.881770148796797 52-53 23.32889217811214 26.328730649566463 27.422347527082426 22.920029645238973 54-55 22.892612906928722 26.439003408087842 27.544553854517 23.123829830466434 56-57 22.89135269494138 26.73617037103667 27.406051164173416 22.966425769848534 58-59 22.94081636474846 26.235615683938967 27.47577458168804 23.347793369624533 60-61 22.736409227788826 26.39588207471807 27.882708520850898 22.985000176642206 62-63 22.697082097064257 26.487468573120708 27.9021334120182 22.913315917796837 64-65 22.521546491226218 26.407014380514624 27.772223711908318 23.29921541635084 66-67 22.47713977125518 26.61383554571571 27.601674041274578 23.307350641754535 68-69 22.808521264621472 26.280586569468234 27.803658879106628 23.107233286803666 70-71 22.728422148477957 26.447550830573203 27.494755135849108 23.32927188509973 72-73 22.75917441302206 26.40819410832695 27.47262898707787 23.360002491573127 74-75 22.67134879802185 26.926284937818522 27.118308984772167 23.284057279387465 76-77 22.87784288449283 26.56093605893118 27.24316236553323 23.31805869104276 78-79 22.86805342643364 26.720261612428807 27.289201099515203 23.12248386162235 80-81 23.012866482083332 26.892229822608975 27.08594206362901 23.00896163167868 82-83 23.04528649305626 26.802154170029972 27.061543304659796 23.091016032253968 84-85 22.90720325185231 26.816804122024678 27.01299928171725 23.26299334440576 86-87 23.01525686184064 26.79556290684701 26.916897919202178 23.272282312110175 88-89 22.87496836464409 26.784372622400127 26.879905459437737 23.46075355351805 90-91 22.793054462352735 26.934652250642856 26.892208706100096 23.380084580904313 92-93 22.9299430309679 26.482258615437654 27.125436928877665 23.46236142471678 94-95 22.755950863178057 26.308545834936897 27.27390309789618 23.66160020398887 96-97 22.926329681932163 26.104632440279797 27.274356605516697 23.69468127227135 98-99 22.70791217196924 26.295091815486916 27.0302650218509 23.966730990692945 100-101 22.924751398215975 25.69959620435791 27.525108245786218 23.850544151639895 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2906.0 1 1724.5 2 687.0 3 820.0 4 677.0 5 607.5 6 986.5 7 2103.0 8 3377.0 9 3752.5 10 3154.0 11 2487.5 12 2418.0 13 2584.0 14 2708.5 15 2831.0 16 2977.0 17 3045.5 18 3157.0 19 3383.5 20 3660.0 21 4124.0 22 4808.5 23 5776.0 24 6894.0 25 8423.0 26 10651.5 27 13286.0 28 15530.5 29 17849.5 30 20748.0 31 24058.0 32 28188.5 33 30074.0 34 30587.5 35 32339.0 36 35034.5 37 38372.0 38 41275.5 39 43361.5 40 45275.5 41 49176.0 42 52899.5 43 55473.0 44 58283.5 45 62777.0 46 64963.5 47 66467.0 48 71596.0 49 72702.5 50 70870.0 51 70048.0 52 69766.5 53 71086.5 54 72288.5 55 69030.0 56 63173.5 57 58241.5 58 54050.0 59 47659.0 60 39614.0 61 33699.5 62 28679.0 63 22189.5 64 16474.5 65 12878.5 66 9918.0 67 8202.5 68 6447.0 69 4636.5 70 3527.5 71 2570.0 72 1972.5 73 1636.0 74 1316.0 75 1090.0 76 802.5 77 523.5 78 342.5 79 236.5 80 166.5 81 131.5 82 90.0 83 54.5 84 43.5 85 32.5 86 20.5 87 21.0 88 21.0 89 14.0 90 8.5 91 8.5 92 6.5 93 5.0 94 4.5 95 4.5 96 4.0 97 2.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.287515762925599 2 0.1769651113913409 3 0.1771227406473308 4 0.19687894073139975 5 0.21001471206389238 6 0.10892181588902901 7 0.12400168137873056 8 0.13624422026061372 9 0.14065783942833124 10-11 0.12760088272383355 12-13 0.1176702395964691 14-15 0.1323297604035309 16-17 0.10889554434636402 18-19 0.13671710802858345 20-21 0.16070302648171503 22-23 0.16582597730138715 24-25 0.1891025641025641 26-27 0.17010823875577974 28-29 0.16708701134930645 30-31 0.15665720891130727 32-33 0.1664302227826818 34-35 0.34610130306851616 36-37 0.46282576712904583 38-39 0.4276744430432955 40-41 0.4171132828919714 42-43 0.44172971836906266 44-45 0.4455916351408155 46-47 0.4493221941992434 48-49 0.4626681378730559 50-51 0.4555485498108449 52-53 0.46227406473308114 54-55 0.4435949978982766 56-57 0.40529108869272806 58-59 0.3948875577973939 60-61 0.3526166456494325 62-63 0.4583333333333333 64-65 0.4567044976881042 66-67 0.4656368221941993 68-69 0.4764606977721732 70-71 0.47110130306851616 72-73 0.46314102564102566 74-75 0.467948717948718 76-77 0.4438577133249264 78-79 0.42996006725514924 80-81 0.42670239596469106 82-83 0.43944409415720886 84-85 0.44151954602774274 86-87 0.4437788986969315 88-89 0.4499527112232031 90-91 0.4686317780580076 92-93 0.4735708280790248 94-95 0.4717843631778058 96-97 0.47026061370323663 98-99 0.46681904161412363 100-101 0.4494272803699033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1903200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.16714643985943 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51767821354038 42.21261959244423 2 13.108327124599208 14.462980041286922 3 4.359090595269533 7.2143576764154504 4 1.9644054379604556 4.334825698528826 5 1.0902461747672676 3.007288518944121 6 0.6665203383608683 2.206201506689921 7 0.42205739587592006 1.6298591515018823 8 0.31558786518174675 1.3928065578499231 9 0.2245529696209244 1.1149151902724557 >10 1.2063514843038907 11.913072952806846 >50 0.08007884637700065 3.01586885390397 >100 0.03919279400958173 3.8077679976290515 >500 0.0042900678385647244 1.6306618167336462 >1k 0.0014300226128549079 1.2821772110998082 >5k 1.9066968171398775E-4 0.7745972338930217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8332 0.43778898696931484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6396 0.3360655737704918 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.2385981504833964 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1921 0.10093526691887349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.2543085329970576E-5 0.0 7 0.0 0.0 0.0 5.2543085329970576E-5 0.0 8 0.0 0.0 0.0 5.2543085329970576E-5 0.0 9 0.0 0.0 0.0 5.2543085329970576E-5 0.0 10-11 0.0 0.0 0.0 5.2543085329970576E-5 0.0 12-13 0.0 0.0 0.0 1.0508617065994115E-4 2.101723413198823E-4 14-15 0.0 0.0 0.0 1.0508617065994115E-4 2.101723413198823E-4 16-17 0.0 0.0 0.0 2.101723413198823E-4 2.101723413198823E-4 18-19 0.0 0.0 0.0 3.6780159730979403E-4 2.101723413198823E-4 20-21 0.0 0.0 0.0 3.6780159730979403E-4 2.364438839848676E-4 22-23 0.0 0.0 0.0 4.728877679697352E-4 3.1525851197982345E-4 24-25 0.0 0.0 0.0 5.779739386296763E-4 3.1525851197982345E-4 26-27 0.0 0.0 0.0 7.881462799495586E-4 3.4153005464480874E-4 28-29 0.0 0.0 0.0 0.0019703656998738966 4.203446826397646E-4 30-31 0.0 0.0 0.0 0.005543295502311895 4.203446826397646E-4 32-33 0.0 0.0 0.0 0.012794241277847835 4.203446826397646E-4 34-35 0.0 0.0 0.0 0.023434216057166876 4.203446826397646E-4 36-37 0.0 0.0 0.0 0.03961748633879782 4.203446826397646E-4 38-39 0.0 0.0 0.0 0.06462799495586381 4.203446826397646E-4 40-41 0.0 0.0 0.0 0.0953394283312316 4.203446826397646E-4 42-43 0.0 0.0 0.0 0.12434321143337537 4.203446826397646E-4 44-45 0.0 0.0 0.0 0.16319882303488858 4.203446826397646E-4 46-47 0.0 0.0 0.0 0.2121164354770912 4.203446826397646E-4 48-49 0.0 0.0 0.0 0.2637400168137873 4.203446826397646E-4 50-51 0.0 0.0 0.0 0.3226408154686843 5.254308532997058E-4 52-53 0.0 0.0 0.0 0.38361706599411516 5.779739386296763E-4 54-55 0.0 0.0 0.0 0.45045187053383773 5.779739386296763E-4 56-57 0.0 0.0 0.0 0.5317885666246323 5.779739386296763E-4 58-59 0.0 0.0 0.0 0.6244220260613703 5.779739386296763E-4 60-61 0.0 0.0 0.0 0.7309005884825557 6.305170239596469E-4 62-63 0.0 0.0 0.0 0.8387189995796553 6.305170239596469E-4 64-65 0.0 0.0 0.0 0.9550231189575451 6.305170239596469E-4 66-67 0.0 0.0 0.0 1.0969945355191257 6.305170239596469E-4 68-69 0.0 0.0 0.0 1.2567255149222363 6.305170239596469E-4 70-71 0.0 0.0 0.0 1.4383932324506095 6.305170239596469E-4 72-73 0.0 0.0 0.0 1.6237389659520807 6.567885666246321E-4 74-75 0.0 0.0 0.0 1.8175178646490122 7.093316519546028E-4 76-77 0.0 0.0 0.0 2.0295292139554437 7.356031946195881E-4 78-79 0.0 0.0 0.0 2.278688524590164 7.618747372845733E-4 80-81 0.0 0.0 0.0 2.5524379991593107 7.881462799495586E-4 82-83 0.0 0.0 0.0 2.846626733921816 8.406893652795292E-4 84-85 0.0 0.0 0.0 3.1453079024800337 8.406893652795292E-4 86-87 0.0 0.0 0.0 3.45541719209752 8.932324506094998E-4 88-89 0.0 0.0 0.0 3.799705758722152 9.457755359394704E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4840 0.0 42.061935 1 GTATCAA 8240 0.0 40.60123 1 ATCAACG 10635 0.0 31.302536 3 TCAACGC 10620 0.0 31.302095 4 TATCAAC 10760 0.0 31.027035 2 CAACGCA 11015 0.0 30.136543 5 AACGCAG 11425 0.0 29.038237 6 ACGCAGA 12880 0.0 25.684315 7 CGCAGAG 12935 0.0 25.501822 8 GCAGAGT 13750 0.0 23.540928 9 GTACATG 8625 0.0 23.38337 1 TACATGG 8600 0.0 22.883873 2 GAGTACT 7270 0.0 22.588417 12-13 ACATGGG 8715 0.0 21.602451 3 GTACTTT 7615 0.0 21.257214 14-15 AGTACTT 7585 0.0 20.900541 12-13 AGAGTAC 12135 0.0 20.736052 10-11 ATCACGC 140 4.907423E-4 20.323715 3 CATGGGG 4800 0.0 19.956758 4 CAGAGTA 13135 0.0 19.608335 10-11 >>END_MODULE