FastQCFastQC Report
Fri 27 May 2016
SRR939237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903200
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83740.43999579655317356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69260.36391340899537616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.2618747372845734No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19150.10062000840689365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50800.045.2515451
GTATCAA83600.037.4601551
ATCAACG108650.028.6535643
TATCAAC109600.028.44852
TCAACGC110550.028.16114
CAACGCA113450.027.441255
GTAAGGT9350.026.9010244
AACGCAG117050.026.7188996
TACCGAC1151.2900066E-424.7604187
GTACATG81500.024.1180611
TAAGGTG11600.023.7287335
TACATGG81800.023.6707172
ACGCAGA133550.023.346737
CGCAGAG134950.023.0693578
ATCACGC1452.3516126E-522.9105013
TATCACG1252.2703869E-422.7795852
ACATGGG83400.021.850923
GAGTACT83150.021.57473612-13
GCAGAGT146400.021.071159
GTACTTT85000.020.65849914-15
Produced by FastQC (version 0.11.5)