##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1903200 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27418505674653 33.0 31.0 34.0 31.0 34.0 2 32.489118327028166 34.0 31.0 34.0 31.0 34.0 3 32.5685755569567 34.0 31.0 34.0 31.0 34.0 4 36.10936212694409 37.0 35.0 37.0 35.0 37.0 5 36.05206967213115 37.0 35.0 37.0 35.0 37.0 6 36.08079445145019 37.0 35.0 37.0 35.0 37.0 7 36.02259615384615 37.0 35.0 37.0 35.0 37.0 8 35.99082177385456 37.0 35.0 37.0 35.0 37.0 9 37.69257461118117 39.0 37.0 39.0 35.0 39.0 10-11 37.60658653846154 39.0 37.0 39.0 35.0 39.0 12-13 37.524780895334175 39.0 37.0 39.0 35.0 39.0 14-15 38.79602248844052 40.0 38.0 41.0 34.5 41.0 16-17 38.79410703026481 40.0 38.0 41.0 34.5 41.0 18-19 38.904246006725515 40.0 38.0 41.0 35.0 41.0 20-21 38.881542927700714 40.0 38.0 41.0 35.0 41.0 22-23 38.79946143337537 40.0 38.0 41.0 34.0 41.0 24-25 38.72090715636822 40.0 38.0 41.0 34.0 41.0 26-27 38.55151455443463 40.0 38.0 41.0 34.0 41.0 28-29 38.36619561790668 40.0 38.0 41.0 34.0 41.0 30-31 38.136743116855826 40.0 37.5 41.0 33.5 41.0 32-33 38.05175152374947 40.0 37.0 41.0 33.0 41.0 34-35 37.94103982765868 40.0 37.0 41.0 33.0 41.0 36-37 37.764112021857926 40.0 37.0 41.0 33.0 41.0 38-39 37.6063545607398 40.0 37.0 41.0 33.0 41.0 40-41 37.39001444934847 40.0 36.5 41.0 32.0 41.0 42-43 37.17214349516604 40.0 36.0 41.0 31.0 41.0 44-45 36.890982030264816 40.0 36.0 41.0 31.0 41.0 46-47 36.53235077763766 39.0 35.5 41.0 30.0 41.0 48-49 36.16646069777217 39.0 35.0 41.0 29.5 41.0 50-51 35.87789564943253 39.0 35.0 40.5 28.5 41.0 52-53 35.58057849936948 39.0 35.0 40.0 28.0 41.0 54-55 35.29046448087432 38.0 35.0 40.0 27.5 41.0 56-57 34.938508039092056 38.0 34.0 40.0 26.5 41.0 58-59 34.577024485077764 37.5 34.0 40.0 26.0 41.0 60-61 34.20584305380412 37.0 33.5 40.0 26.0 41.0 62-63 33.82145465531735 36.5 33.0 39.5 25.0 41.0 64-65 33.472715163934424 36.0 33.0 39.0 24.5 40.5 66-67 33.025695933165196 35.5 32.5 39.0 23.0 40.0 68-69 32.54027611391341 35.0 32.0 38.0 22.0 40.0 70-71 32.036476986128626 35.0 31.0 37.5 20.5 40.0 72-73 31.536630674653217 35.0 31.0 37.0 19.0 39.0 74-75 31.00924154056326 34.5 30.5 36.0 17.5 39.0 76-77 29.12369456704498 32.5 28.0 35.0 11.0 36.5 78-79 30.08114018495166 34.0 30.0 35.0 11.5 37.0 80-81 30.095611864228665 34.0 30.0 35.0 9.0 37.0 82-83 29.732496321984026 34.0 30.0 35.0 7.0 36.5 84-85 29.270326029844473 34.0 29.0 35.0 2.0 36.0 86-87 28.64676728667507 34.0 29.0 35.0 2.0 35.5 88-89 28.030238020176544 33.0 27.0 35.0 2.0 35.0 90-91 27.508432377049182 33.0 26.5 35.0 2.0 35.0 92-93 27.106551334594368 33.0 26.0 35.0 2.0 35.0 94-95 26.538096364018493 32.5 25.0 34.0 2.0 35.0 96-97 25.742390447667088 32.0 23.5 34.0 2.0 35.0 98-99 24.650301071878943 32.0 10.5 34.0 2.0 35.0 100-101 20.318549548129468 27.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21861748849756424 1101 2 0.28505586059268495 1101 3 0.26306242231154187 1101 4 0.17177649730041367 1101 5 0.17707940849071946 1101 6 0.16450875056764858 1101 7 0.11324788308979095 1101 8 0.2277621585721903 1101 9 0.24814837176091942 1101 10-11 0.24170466372082444 1101 12-13 0.24734453255727828 1101 14-15 0.23125002403235584 1101 16-17 0.1831739173776512 1101 18-19 0.26616546093234916 1101 20-21 0.34976733575431496 1101 22-23 0.33994103132625497 1101 24-25 0.40954785086724854 1101 26-27 0.28962878613563703 1101 28-29 0.24199773294739657 1101 30-31 0.2863200147222571 1101 32-33 0.37328823473695394 1101 34-35 0.34659561681151985 1101 36-37 0.34041302350444624 1101 38-39 0.4010814171240682 1101 40-41 0.4310680185194897 1101 42-43 0.3462102530328153 1101 44-45 0.38833472843109007 1101 46-47 0.4296865265014205 1101 48-49 0.38525500392660206 1101 50-51 0.5716141607718299 1101 52-53 0.5351393996344527 1101 54-55 0.40973235133053265 1101 56-57 0.4060358328404732 1101 58-59 0.5349499414464205 1101 60-61 0.5181626712802796 1101 62-63 0.4169082835521536 1101 64-65 0.3301391806224174 1101 66-67 0.41948990561622423 1101 68-69 0.3183581621986278 1101 70-71 0.36710116630505496 1101 72-73 0.5493903330366798 1101 74-75 0.48888920911222655 1101 76-77 0.35450276245973456 1101 78-79 0.509628179049102 1101 80-81 0.5019927721866146 1101 82-83 0.5362542056087385 1101 84-85 0.6777309196313617 1101 86-87 0.4262848021743082 1101 88-89 0.470577056141547 1101 90-91 0.5644929488145038 1101 92-93 0.756750879935101 1101 94-95 0.43189554703680244 1101 96-97 0.4540150263349041 1101 98-99 0.35460298940396484 1101 100-101 0.3955482080619248 1102 1 0.26619758411116834 1102 2 0.325855371653482 1102 3 0.2856983985857937 1102 4 0.18785859348143674 1102 5 0.2502976572716875 1102 6 0.2588575784658218 1102 7 0.21394045858502153 1102 8 0.3107625059205432 1102 9 0.28219646492451744 1102 10-11 0.285768188696764 1102 12-13 0.3337828994366845 1102 14-15 0.39251279438736475 1102 16-17 0.3938181557699849 1102 18-19 0.4286336759065108 1102 20-21 0.4452775268489333 1102 22-23 0.4988436226510302 1102 24-25 0.3976597871884806 1102 26-27 0.4955486891962835 1102 28-29 0.4530161662737484 1102 30-31 0.5276856903413218 1102 32-33 0.6306511580843406 1102 34-35 0.5789415041804986 1102 36-37 0.5298812755997986 1102 38-39 0.5594772378074779 1102 40-41 0.703018909825964 1102 42-43 0.7189667406250493 1102 44-45 0.7741983133312544 1102 46-47 0.6967539916273751 1102 48-49 0.6713116190566737 1102 50-51 0.5086857755174279 1102 52-53 0.47464278429226425 1102 54-55 0.5944812396832191 1102 56-57 0.43789675827193975 1102 58-59 0.49464957792383046 1102 60-61 0.5368909785801605 1102 62-63 0.3933277053629709 1102 64-65 0.343238654827168 1102 66-67 0.48339736246946785 1102 68-69 0.21368268101510068 1102 70-71 0.05362656913633401 1102 72-73 0.25728130542647065 1102 74-75 0.1800474700371275 1102 76-77 0.07617862546130638 1102 78-79 0.299792983910919 1102 80-81 0.3818858101059206 1102 82-83 0.4611003515003489 1102 84-85 0.5558991475979411 1102 86-87 0.3959455144240387 1102 88-89 0.5878203829433168 1102 90-91 0.5862267474001897 1102 92-93 0.5156036227939147 1102 94-95 0.5770715810610305 1102 96-97 0.3473161084178287 1102 98-99 0.2981638535854536 1102 100-101 0.4477588416942595 1103 1 0.20162601831221139 1103 2 0.24018169024785863 1103 3 0.2761635294007547 1103 4 0.19075616206347235 1103 5 0.2405808676250345 1103 6 0.3052321635522901 1103 7 0.23068536530555406 1103 8 0.2219527526152163 1103 9 0.3360597252155273 1103 10-11 0.2644188520649493 1103 12-13 0.35261577933957255 1103 14-15 0.4405598634283123 1103 16-17 0.35275841151133847 1103 18-19 0.3285869621532598 1103 20-21 0.33520709435195073 1103 22-23 0.39254894440022525 1103 24-25 0.359564063470728 1103 26-27 0.38365526892711443 1103 28-29 0.35642314504531214 1103 30-31 0.3923926273336775 1103 32-33 0.4915828342856585 1103 34-35 0.43242398158847806 1103 36-37 0.6040919941033849 1103 38-39 0.6831749274505015 1103 40-41 0.5646554044522389 1103 42-43 0.6058918242656972 1103 44-45 0.7964588971215818 1103 46-47 0.7595724757989899 1103 48-49 0.7336526031378128 1103 50-51 0.7784006242405326 1103 52-53 0.5956261416952415 1103 54-55 0.6421291035159271 1103 56-57 0.7683866934319425 1103 58-59 0.668065011226787 1103 60-61 0.7263358086506528 1103 62-63 0.7911199870666437 1103 64-65 0.6712366415301929 1103 66-67 0.6013092905960775 1103 68-69 0.5558091108013699 1103 70-71 0.5419180300596267 1103 72-73 0.6005306746263166 1103 74-75 0.5352198668145398 1103 76-77 0.6723005615160957 1103 78-79 0.7884288765219196 1103 80-81 0.9178986596777641 1103 82-83 0.9360000978644045 1103 84-85 0.7471863746761258 1103 86-87 0.7040748588853312 1103 88-89 0.9235909460414256 1103 90-91 1.0896324102913724 1103 92-93 0.9567783421001828 1103 94-95 0.7394689835131736 1103 96-97 0.5100434678735795 1103 98-99 0.3072894839743938 1103 100-101 0.17932253523888164 1104 1 0.15120731252830666 1104 2 0.125074050214387 1104 3 0.11587338309144712 1104 4 0.08013605911446575 1104 5 0.18667622224126035 1104 6 0.15726483591581086 1104 7 0.11761134851929 1104 8 0.104232401168602 1104 9 0.13220230705491787 1104 10-11 0.20440699771498316 1104 12-13 0.1991005208260006 1104 14-15 0.2083463012912361 1104 16-17 0.1934400683310784 1104 18-19 0.1721108103161484 1104 20-21 0.25550766868570207 1104 22-23 0.22829456399603743 1104 24-25 0.20712060935403542 1104 26-27 0.19433828868014302 1104 28-29 0.23442872990217012 1104 30-31 0.30567203499653317 1104 32-33 0.08290063229259914 1104 34-35 0.22340627613420594 1104 36-37 0.28012988364873337 1104 38-39 0.22914222973009402 1104 40-41 0.3148690363207933 1104 42-43 0.3210430464681977 1104 44-45 0.29000794370220717 1104 46-47 0.3903942611782014 1104 48-49 0.3420916958679925 1104 50-51 0.2663478565350843 1104 52-53 0.2310316618920112 1104 54-55 0.2124024191839453 1104 56-57 0.35991479521452874 1104 58-59 0.34001378395291226 1104 60-61 0.5116072542474299 1104 62-63 0.6041936868916835 1104 64-65 0.5334171446110574 1104 66-67 0.5445914558216245 1104 68-69 0.5315663311781691 1104 70-71 0.3464966934975138 1104 72-73 0.32716144015107673 1104 74-75 0.32693259851319567 1104 76-77 0.37491818582828884 1104 78-79 0.5125871859775089 1104 80-81 0.6294152874638534 1104 82-83 0.7995413155026654 1104 84-85 0.7152003907016855 1104 86-87 0.5890237964312917 1104 88-89 0.8636676823550395 1104 90-91 1.0150118128828467 1104 92-93 0.5959119602760055 1104 94-95 0.5178549218451991 1104 96-97 0.41651042783503556 1104 98-99 0.5489011050409047 1104 100-101 0.40474456911569945 1105 1 0.13626642650808662 1105 2 0.03196983445742774 1105 3 0.06671969158247748 1105 4 0.09306988822946494 1105 5 0.04979566707547178 1105 6 0.126974515754398 1105 7 0.10862389466617373 1105 8 0.07007493565478029 1105 9 0.11311515657372695 1105 10-11 0.12317486980523995 1105 12-13 0.1443558286251161 1105 14-15 0.1494411316303612 1105 16-17 0.09156098889794606 1105 18-19 0.13155114475104313 1105 20-21 0.11470659382008819 1105 22-23 0.07345149969764009 1105 24-25 0.14323983161872889 1105 26-27 0.10852488781792147 1105 28-29 0.15608350401829796 1105 30-31 0.1490605107340386 1105 32-33 0.18693736593979082 1105 34-35 0.2973563588720083 1105 36-37 0.12005809893151564 1105 38-39 0.17195486732401832 1105 40-41 0.2747055203030442 1105 42-43 0.09258284649432369 1105 44-45 0.06397242088156929 1105 46-47 0.1901544243797133 1105 48-49 0.1413857029418324 1105 50-51 0.05019475289994091 1105 52-53 0.11508171342168794 1105 54-55 0.13314725895808976 1105 56-57 0.1710532201481172 1105 58-59 0.35287155840289586 1105 60-61 0.10296841199599527 1105 62-63 0.2764761009967813 1105 64-65 0.2696126937045662 1105 66-67 0.16679436785344848 1105 68-69 0.22751973915293178 1105 70-71 0.15914290594495384 1105 72-73 0.13625032038448026 1105 74-75 0.2467501157018539 1105 76-77 0.2780652159767847 1105 78-79 0.2827997338178605 1105 80-81 0.2880805900858192 1105 82-83 0.45743805776232804 1105 84-85 0.5392726447502731 1105 86-87 0.5864241784283202 1105 88-89 0.6212645303352211 1105 90-91 0.5741127892856426 1105 92-93 0.680801364999315 1105 94-95 0.8753879020510276 1105 96-97 0.627424378105264 1105 98-99 0.3677661093713347 1105 100-101 0.08159192634460766 1106 1 0.09867482026344021 1106 2 0.08584822192025143 1106 3 0.1086936975751911 1106 4 0.0773159092080391 1106 5 0.019902384392239014 1106 6 0.06638700401038022 1106 7 0.055913662946608156 1106 8 0.10013148705915853 1106 9 0.09919867694949147 1106 10-11 0.18345806840798673 1106 12-13 0.20304831925169964 1106 14-15 0.29702908321218047 1106 16-17 0.1845734653009714 1106 18-19 0.17360141060016332 1106 20-21 0.2669731239065598 1106 22-23 0.2579137791032977 1106 24-25 0.10501272657069904 1106 26-27 0.22915432650471246 1106 28-29 0.11788065686745597 1106 30-31 0.1853025354056257 1106 32-33 0.21595198982036834 1106 34-35 0.37831614654133006 1106 36-37 0.23536808580610824 1106 38-39 0.2982029869738412 1106 40-41 0.3227134966482552 1106 42-43 0.38586329276990483 1106 44-45 0.6442409311096924 1106 46-47 0.5994383138241304 1106 48-49 0.599207901561428 1106 50-51 0.5666626694341801 1106 52-53 0.5392432471947828 1106 54-55 0.6860280222422688 1106 56-57 0.7665703607492134 1106 58-59 0.5707902714967616 1106 60-61 0.5552884256617574 1106 62-63 0.6038580667173292 1106 64-65 0.6666371938902032 1106 66-67 0.6625302071970296 1106 68-69 0.57676925501179 1106 70-71 0.6484023225120268 1106 72-73 0.5569307732837778 1106 74-75 0.6458846171715642 1106 76-77 0.7324997338895685 1106 78-79 0.7915125313547691 1106 80-81 0.8580528576443243 1106 82-83 0.870063871999708 1106 84-85 0.9402366564893541 1106 86-87 1.0201925657691255 1106 88-89 1.1993592715136252 1106 90-91 1.157959557233145 1106 92-93 1.2221186646857376 1106 94-95 1.3957370821341009 1106 96-97 1.1569678822471197 1106 98-99 1.3884740739216852 1106 100-101 1.1919158259221447 1107 1 0.08888832620489495 1107 2 0.13025276530460417 1107 3 0.09508065811731115 1107 4 0.10704388580105473 1107 5 0.08338665782337529 1107 6 0.10215249250700253 1107 7 0.13363479410025292 1107 8 0.08512396535662958 1107 9 0.19932620086022723 1107 10-11 0.13925902621744513 1107 12-13 0.09984310788495065 1107 14-15 0.04491293156362275 1107 16-17 0.13843920636118412 1107 18-19 0.18501162631431356 1107 20-21 0.22712314391842625 1107 22-23 0.26298023231002077 1107 24-25 0.2321536515330962 1107 26-27 0.11529148371519682 1107 28-29 0.060110117099043237 1107 30-31 0.18287005821225222 1107 32-33 0.1952601312007758 1107 34-35 0.2456647809037733 1107 36-37 0.21214617487962784 1107 38-39 0.32155545809464314 1107 40-41 0.40686133605645836 1107 42-43 0.4032475344580817 1107 44-45 0.4755114777787455 1107 46-47 0.3745272631319949 1107 48-49 0.0312524245198631 1107 50-51 0.2719569819229619 1107 52-53 0.3087069291026765 1107 54-55 0.41026142964251733 1107 56-57 0.4075077443008368 1107 58-59 0.4108603820450867 1107 60-61 0.3973870496732417 1107 62-63 0.44293779676411305 1107 64-65 0.4997299247512714 1107 66-67 0.601909149190206 1107 68-69 0.42129193743032545 1107 70-71 0.49761854730741817 1107 72-73 0.3934782428174337 1107 74-75 0.41164527521702254 1107 76-77 0.37602251291713173 1107 78-79 0.4275060171463423 1107 80-81 0.6028664712358349 1107 82-83 0.8084787939534195 1107 84-85 0.6735972703706885 1107 86-87 0.68390814880458 1107 88-89 0.7567022473475689 1107 90-91 0.8018193637390709 1107 92-93 0.815781802822837 1107 94-95 0.9601321026290144 1107 96-97 0.8831674212840639 1107 98-99 1.1445938415079802 1107 100-101 1.0596016257758478 1108 1 0.015278714340247745 1108 2 0.04628009368092734 1108 3 0.08668314958557488 1108 4 0.0038920041256815807 1108 5 0.04424351908291868 1108 6 0.07095737889578402 1108 7 0.06282587147925511 1108 8 0.09690279055827489 1108 9 0.08366280385454417 1108 10-11 0.04903689935102307 1108 12-13 0.036219349093407516 1108 14-15 0.11049875513601393 1108 16-17 0.14565011625665392 1108 18-19 0.2444320716407944 1108 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-0.4764093171162891 2316 96-97 -0.30888528329291276 2316 98-99 -0.27607946576727116 2316 100-101 0.20595391426406096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 13.0 5 32.0 6 119.0 7 290.0 8 683.0 9 1253.0 10 2099.0 11 3121.0 12 4490.0 13 6136.0 14 7862.0 15 10663.0 16 13529.0 17 18609.0 18 18106.0 19 13283.0 20 12320.0 21 12835.0 22 13786.0 23 15136.0 24 17288.0 25 20108.0 26 23502.0 27 28425.0 28 34271.0 29 41062.0 30 50245.0 31 61109.0 32 74845.0 33 97340.0 34 132352.0 35 192408.0 36 293974.0 37 415477.0 38 253323.0 39 13092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61428754349163 19.265678458872188 15.542536828576983 22.5774971690592 2 16.03509878100042 20.381357713324928 39.09967423287096 24.483869272803698 3 19.52322404371585 24.119482976040352 29.71132828919714 26.645964691046657 4 13.628205128205128 16.962116435477093 35.73891340899537 33.6707650273224 5 14.28709541824296 37.08322824716267 33.28562421185372 15.344052122740647 6 32.74668978562421 35.585224884405214 16.69377889869693 14.974306431273646 7 29.150115594787724 32.7526271542665 19.7005569567045 18.396700294241278 8 27.14664775115595 34.592738545607396 18.96227406473308 19.298339638503574 9 26.698139974779316 16.818148381672973 19.679697351828498 36.80401429171921 10-11 25.61979823455233 26.391156998738968 26.189627994955867 21.79941677175284 12-13 26.54615910046238 23.62442202606137 27.070328919714164 22.759089953762086 14-15 23.143184941556765 25.015375650591658 25.581640960890805 26.25979844696077 16-17 22.54119604148096 28.193190735642563 26.375581163485556 22.890032059390922 18-19 22.558025345228046 26.235098440861528 29.114934674444097 22.091941539466326 20-21 23.25970030992547 25.36381498629019 29.279011475150135 22.0974732286342 22-23 22.86228457335015 25.272304539722573 29.345418242959227 22.519992643968052 24-25 22.78930222782682 25.57103825136612 29.258774695250107 22.380884825556958 26-27 22.35454930691602 25.885109603763983 29.162516849941667 22.59782423937833 28-29 22.345996216897856 26.11714480874317 29.204707860445566 22.332151113913408 30-31 22.56079234972678 25.75073560319462 29.01003572929802 22.67843631778058 32-33 22.2781368221942 25.844498738965953 28.966661412358135 22.910703026481716 34-35 22.994364680074085 25.914090927003556 28.684093685551776 22.407450707370575 36-37 23.13890230297884 25.490355780199963 28.751240766829373 22.61950114999183 38-39 22.67749369725159 25.812473924644458 28.706483712689252 22.8035486654147 40-41 22.828169617980432 25.736527956101916 28.87148602923964 22.563816396678007 42-43 22.727066136926027 25.755446563424517 28.505400522437135 23.012086777212325 44-45 22.788649811853926 25.95816833497489 28.506041613082978 22.74714024008821 46-47 23.123466231679178 26.39262419828956 27.783258323562492 22.70065124646877 48-49 23.047058285733502 26.34396511546648 27.611545439690037 22.99743115910998 50-51 23.04505569567045 26.588141025641026 27.5599516603615 22.80685161832703 52-53 23.38649909612686 26.399794871633205 27.438677573568913 22.77502845867102 54-55 22.945591635140815 26.474385245901637 27.478772593526692 23.101250525430853 56-57 22.977836089416474 26.763124322188503 27.317484804411972 22.94155478398305 58-59 22.980712594165805 26.318872989592567 27.4622614295495 23.238152986692125 60-61 22.810481263922373 26.437477357053552 27.78613656622722 22.965904812796854 62-63 22.745604519601834 26.598636542643312 27.80596019339247 22.849798744362385 64-65 22.503754207391978 26.467661273288435 27.713643036615217 23.314941482704373 66-67 22.53318834872284 26.676099949006684 27.56660460421592 23.224107098054557 68-69 22.84370287467751 26.374338603487864 27.714707566848993 23.06725095498563 70-71 22.71453341740227 26.506278898696934 27.453157839428332 23.32602984447247 72-73 22.768836696090794 26.521306221101305 27.351381883144178 23.358475199663726 74-75 22.715952080706177 26.95179171920975 26.998003362757462 23.33425283732661 76-77 22.7906775837488 26.62899576241835 27.24365449210257 23.336672161730284 78-79 22.824786799695353 26.775819758159752 27.26746579508128 23.131927647063613 80-81 23.012258587066547 26.89856249735642 27.000890344218263 23.088288571358763 82-83 22.967633459436737 26.87008722152165 27.0249842370744 23.137295081967213 84-85 22.84901744430433 26.927280369903322 26.896069777217317 23.32763240857503 86-87 22.994925099390155 26.813710883987884 26.842218902130977 23.349145114490987 88-89 22.759144227214644 26.89625476796057 26.756769014228603 23.587831990596182 90-91 22.684171483845677 26.98833761487997 26.853806080709436 23.473684820564912 92-93 22.810135561160152 26.60232765868012 26.919766708701136 23.667770071458598 94-95 22.54960067255149 26.5171815889029 27.189601723413197 23.74361601513241 96-97 22.717791088692728 26.240384615384617 27.157734342160573 23.884089953762086 98-99 22.544203922190903 26.475484796236227 26.7771352038531 24.203176077719775 100-101 22.67115420587265 25.788857269561305 27.54527121746788 23.994717307098163 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 982.0 1 671.0 2 479.0 3 630.5 4 545.0 5 467.5 6 832.0 7 1953.5 8 3169.0 9 3518.5 10 2933.5 11 2191.0 12 2050.5 13 2194.5 14 2396.0 15 2539.5 16 2628.0 17 2798.0 18 3002.5 19 3181.5 20 3414.5 21 3974.5 22 4718.5 23 5709.0 24 7007.0 25 8533.0 26 10765.0 27 13360.5 28 15493.5 29 17951.0 30 20850.5 31 24155.5 32 28504.0 33 30336.0 34 30711.0 35 32564.0 36 35338.5 37 38593.0 38 41439.5 39 43533.5 40 45475.0 41 49594.0 42 53423.0 43 55924.5 44 58994.5 45 63611.0 46 65908.0 47 67660.0 48 72272.5 49 73249.5 50 71623.0 51 70456.5 52 69774.5 53 71405.0 54 73079.5 55 69546.0 56 63548.5 57 58355.5 58 53595.5 59 47352.5 60 39399.0 61 33256.0 62 28292.0 63 21723.5 64 15975.0 65 12414.5 66 9549.5 67 7970.5 68 6237.5 69 4368.0 70 3229.0 71 2368.0 72 1827.0 73 1595.0 74 1318.5 75 1082.0 76 801.0 77 480.0 78 305.0 79 196.0 80 120.0 81 83.5 82 54.5 83 36.0 84 17.5 85 8.0 86 6.0 87 4.0 88 5.0 89 5.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2848360655737705 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014974779319041614 16-17 7.093316519546028E-4 18-19 7.881462799495586E-5 20-21 2.6271542664985288E-5 22-23 0.0 24-25 0.0 26-27 1.8390079865489702E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013135771332492643 36-37 0.007513661202185792 38-39 0.003257671290458176 40-41 0.0033890290037831023 42-43 2.364438839848676E-4 44-45 0.001287305590584279 46-47 1.8390079865489702E-4 48-49 3.6780159730979403E-4 50-51 0.0 52-53 7.881462799495586E-5 54-55 0.0 56-57 8.406893652795292E-4 58-59 0.0021542664985287935 60-61 7.093316519546028E-4 62-63 0.0012347625052543086 64-65 1.0508617065994115E-4 66-67 4.991593106347205E-4 68-69 0.0015762925598991173 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2537568306010929 78-79 7.881462799495586E-5 80-81 1.8390079865489702E-4 82-83 0.0 84-85 0.0 86-87 0.10406158049600672 88-89 0.36512189995796557 90-91 0.269651113913409 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.8390079865489702E-4 100-101 2.101723413198823E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1903200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.520998454025936 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91802099647053 40.096872859307815 2 14.050228520466474 15.039645178451947 3 4.68028436030391 7.514804760366821 4 2.080744881628974 4.454541743715468 5 1.1069608941146982 2.962282615128997 6 0.7040016295165925 2.2607322074993568 7 0.47001409529876215 1.7608936567498814 8 0.3295313506048077 1.4109477525018395 9 0.24134776847146178 1.1625456188919343 >10 1.282868690887356 12.380088778374626 >50 0.08722633398362935 3.1917477493138398 >100 0.04288400521328819 4.083171271121084 >500 0.004218639011679044 1.5877465521875123 >1k 0.001471618259888038 1.2906019745425246 >5k 1.9621576798507174E-4 0.8033772818463171 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8374 0.43999579655317356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6926 0.36391340899537616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4984 0.2618747372845734 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1915 0.10062000840689365 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2543085329970576E-5 2 0.0 0.0 0.0 0.0 5.2543085329970576E-5 3 0.0 0.0 0.0 0.0 5.2543085329970576E-5 4 0.0 0.0 0.0 5.2543085329970576E-5 5.2543085329970576E-5 5 0.0 0.0 0.0 5.2543085329970576E-5 5.2543085329970576E-5 6 0.0 0.0 0.0 5.2543085329970576E-5 5.2543085329970576E-5 7 0.0 0.0 0.0 5.2543085329970576E-5 5.2543085329970576E-5 8 0.0 0.0 0.0 5.2543085329970576E-5 5.2543085329970576E-5 9 0.0 0.0 0.0 1.0508617065994115E-4 5.2543085329970576E-5 10-11 0.0 0.0 0.0 1.3135771332492644E-4 5.2543085329970576E-5 12-13 0.0 0.0 0.0 2.101723413198823E-4 5.2543085329970576E-5 14-15 0.0 0.0 0.0 2.101723413198823E-4 1.0508617065994115E-4 16-17 0.0 0.0 0.0 3.940731399747793E-4 1.5762925598991173E-4 18-19 0.0 0.0 0.0 7.356031946195881E-4 1.5762925598991173E-4 20-21 0.0 0.0 0.0 7.356031946195881E-4 2.8898696931483817E-4 22-23 0.0 0.0 0.0 7.881462799495586E-4 3.1525851197982345E-4 24-25 0.0 0.0 0.0 8.406893652795292E-4 3.1525851197982345E-4 26-27 0.0 0.0 0.0 0.0011296763345943674 4.466162253047499E-4 28-29 0.0 0.0 0.0 0.002338167297183691 4.728877679697352E-4 30-31 0.0 0.0 0.0 0.006016183270281631 4.728877679697352E-4 32-33 0.0 0.0 0.0 0.01316204287515763 4.728877679697352E-4 34-35 0.0 0.0 0.0 0.02390710382513661 4.728877679697352E-4 36-37 0.0 0.0 0.0 0.04024800336275746 4.728877679697352E-4 38-39 0.0 0.0 0.0 0.06531105506515342 4.728877679697352E-4 40-41 0.0 0.0 0.0 0.0960750315258512 4.728877679697352E-4 42-43 0.0 0.0 0.0 0.12547288776796972 4.728877679697352E-4 44-45 0.0 0.0 0.0 0.16443358554014292 4.728877679697352E-4 46-47 0.0 0.0 0.0 0.21361391340899538 4.728877679697352E-4 48-49 0.0 0.0 0.0 0.26557902480033624 6.042454812946617E-4 50-51 0.0 0.0 0.0 0.32513661202185795 6.305170239596469E-4 52-53 0.0 0.0 0.0 0.38653320722992857 6.305170239596469E-4 54-55 0.0 0.0 0.0 0.4541036149642707 6.830601092896175E-4 56-57 0.0 0.0 0.0 0.5358869272803699 6.830601092896175E-4 58-59 0.0 0.0 0.0 0.6289407313997477 6.830601092896175E-4 60-61 0.0 0.0 0.0 0.7354455653635982 6.830601092896175E-4 62-63 0.0 0.0 0.0 0.8437631357713324 6.830601092896175E-4 64-65 0.0 0.0 0.0 0.9600672551492224 6.830601092896175E-4 66-67 0.0 0.0 0.0 1.1010403530895334 6.830601092896175E-4 68-69 0.0 0.0 0.0 1.2601145439260193 6.830601092896175E-4 70-71 0.0 0.0 0.0 1.4421763345943672 6.830601092896175E-4 72-73 0.0 0.0 0.0 1.6277585119798235 7.881462799495586E-4 74-75 0.0 0.0 0.0 1.8210645229087852 8.932324506094998E-4 76-77 0.0 0.0 0.0 2.033627574611181 8.932324506094998E-4 78-79 0.0 0.0 0.0 2.2827606137032364 9.457755359394704E-4 80-81 0.0 0.0 0.0 2.5564838167297186 9.457755359394704E-4 82-83 0.0 0.0 0.0 2.849227616645649 9.457755359394704E-4 84-85 0.0 0.0 0.0 3.1476460697772173 9.457755359394704E-4 86-87 0.0 0.0 0.0 3.4584384195039934 9.457755359394704E-4 88-89 0.0 0.0 0.0 3.801282051282051 9.457755359394704E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5080 0.0 45.251545 1 GTATCAA 8360 0.0 37.460155 1 ATCAACG 10865 0.0 28.653564 3 TATCAAC 10960 0.0 28.4485 2 TCAACGC 11055 0.0 28.1611 4 CAACGCA 11345 0.0 27.44125 5 GTAAGGT 935 0.0 26.901024 4 AACGCAG 11705 0.0 26.718899 6 TACCGAC 115 1.2900066E-4 24.760418 7 GTACATG 8150 0.0 24.118061 1 TAAGGTG 1160 0.0 23.728733 5 TACATGG 8180 0.0 23.670717 2 ACGCAGA 13355 0.0 23.34673 7 CGCAGAG 13495 0.0 23.069357 8 ATCACGC 145 2.3516126E-5 22.910501 3 TATCACG 125 2.2703869E-4 22.779585 2 ACATGGG 8340 0.0 21.85092 3 GAGTACT 8315 0.0 21.574736 12-13 GCAGAGT 14640 0.0 21.07115 9 GTACTTT 8500 0.0 20.658499 14-15 >>END_MODULE