FastQCFastQC Report
Fri 27 May 2016
SRR939236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936469
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.43894325186718713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65160.3364887328431284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.23486045994023141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.045.800521
GTATCAA83250.038.338341
ATCAACG107650.029.6033343
TATCAAC108050.029.5376322
TCAACGC109750.028.9936834
CAACGCA112200.028.3183125
AACGCAG114750.027.716016
ACGCAGA129600.024.3573557
CGCAGAG131450.024.0506138
GTACATG86900.023.6655391
TACATGG87700.022.7109382
GAGTACT75900.022.54375812-13
GCAGAGT141000.022.1527279
ACATGGG88650.021.6116543
AGTACTT78850.021.61016512-13
GTACTTT80200.021.24722714-15
AGAGTAC124050.020.53726410-11
TATAGGG3302.910383E-1120.1186812
TACTTTT87550.019.21984914-15
CATGGGG46000.019.0721344
Produced by FastQC (version 0.11.5)