##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936469 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.841521346326743 33.0 31.0 34.0 30.0 34.0 2 31.93233818873424 33.0 31.0 34.0 30.0 34.0 3 31.981810191642623 34.0 31.0 34.0 30.0 34.0 4 35.47997411784026 37.0 35.0 37.0 33.0 37.0 5 35.519297752765475 37.0 35.0 37.0 33.0 37.0 6 35.60331407319198 37.0 35.0 37.0 33.0 37.0 7 35.520232960093864 37.0 35.0 37.0 33.0 37.0 8 35.49934804017002 37.0 35.0 37.0 33.0 37.0 9 37.125528474765154 39.0 37.0 39.0 33.0 39.0 10-11 37.032982454147216 39.0 37.0 39.0 33.0 39.0 12-13 36.924402352942394 39.0 37.0 39.0 33.0 39.0 14-15 38.156104487084484 40.0 38.0 41.0 33.0 41.0 16-17 38.085806950692216 40.0 38.0 41.0 33.0 41.0 18-19 38.022214917976996 40.0 38.0 41.0 33.0 41.0 20-21 37.917202909006036 40.0 38.0 41.0 33.0 41.0 22-23 37.837101445982356 40.0 38.0 41.0 32.5 41.0 24-25 37.85051813377854 40.0 38.0 41.0 33.0 41.0 26-27 37.76080019871219 40.0 38.0 41.0 32.0 41.0 28-29 37.6082465043334 40.0 37.5 41.0 32.0 41.0 30-31 37.40058245187504 40.0 37.0 41.0 31.5 41.0 32-33 37.176964619624684 40.0 36.5 41.0 31.0 41.0 34-35 36.862992642794694 40.0 36.0 41.0 30.5 41.0 36-37 36.59718281056914 40.0 36.0 41.0 30.0 41.0 38-39 36.254153306869355 39.0 35.0 41.0 29.5 41.0 40-41 36.097749305565955 39.0 35.0 41.0 29.5 41.0 42-43 35.91186535906333 39.0 35.0 41.0 28.5 41.0 44-45 35.840060181701844 39.0 35.0 41.0 28.0 41.0 46-47 35.61447691649079 39.0 35.0 41.0 26.5 41.0 48-49 35.374649684554726 39.0 35.0 41.0 26.0 41.0 50-51 34.201536662864214 38.0 34.0 40.0 24.0 40.5 52-53 34.2961431347468 38.0 33.5 39.5 24.0 40.5 54-55 34.75689179635718 39.0 35.0 40.0 24.0 41.0 56-57 34.62412359815727 38.0 34.0 40.0 23.5 41.0 58-59 34.3890937577622 38.0 34.0 40.0 23.0 41.0 60-61 34.07211347044543 37.5 34.0 40.0 22.5 41.0 62-63 33.697741869350864 37.0 34.0 40.0 22.0 41.0 64-65 33.30294210751631 36.5 33.0 39.5 20.5 41.0 66-67 32.93156358299565 36.0 33.0 39.0 19.5 41.0 68-69 32.51281172071435 35.5 32.5 39.0 18.0 40.5 70-71 32.08605998856682 35.0 32.0 38.5 15.5 40.0 72-73 31.658836005120662 35.0 32.0 37.0 12.0 40.0 74-75 31.220484293835845 35.0 31.0 37.0 7.5 39.0 76-77 30.79273254567979 35.0 31.0 36.0 7.0 39.0 78-79 30.37873547162387 35.0 31.0 36.0 2.0 38.5 80-81 29.945169016390143 34.0 30.0 35.5 2.0 37.0 82-83 29.516299253951395 34.0 30.0 35.0 2.0 37.0 84-85 29.118492730841545 34.0 29.0 35.0 2.0 36.0 86-87 28.80524371936757 34.0 29.0 35.0 2.0 36.0 88-89 28.400364013056752 34.0 29.0 35.0 2.0 36.0 90-91 28.0149986909163 34.0 28.5 35.0 2.0 35.0 92-93 27.606719498220734 34.0 27.0 35.0 2.0 35.0 94-95 27.136784787156415 34.0 26.0 35.0 2.0 35.0 96-97 26.577361682526288 33.0 25.0 35.0 2.0 35.0 98-99 25.83772913483252 33.0 23.5 35.0 2.0 35.0 100-101 23.378489921604736 30.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08868697144512794 1101 2 0.03949348811157094 1101 3 0.19164965988128912 1101 4 0.19954181558993156 1101 5 0.16739968271337347 1101 6 0.2847532817335434 1101 7 0.29803627262641896 1101 8 0.23939449425353843 1101 9 0.2990516498908846 1101 10-11 0.30900745726811607 1101 12-13 0.32768987772544733 1101 14-15 0.26520448475951497 1101 16-17 0.39790822367006484 1101 18-19 0.2987759276332014 1101 20-21 0.41141339147385025 1101 22-23 0.374182727863726 1101 24-25 0.4703212641866301 1101 26-27 0.3642344525125054 1101 28-29 0.40220257530878456 1101 30-31 0.45237481333561647 1101 32-33 0.4836124358611258 1101 34-35 0.3853485192731938 1101 36-37 0.28034845019171684 1101 38-39 0.4665050670879083 1101 40-41 0.32155880944774395 1101 42-43 0.15551971281760046 1101 44-45 0.34251686656999425 1101 46-47 0.3809053220238141 1101 48-49 0.30803583783616517 1101 50-51 0.40473871931519767 1101 52-53 0.3670775224034699 1101 54-55 0.32473900158078095 1101 56-57 0.28356624419893706 1101 58-59 0.3217312417188154 1101 60-61 0.30322156985992166 1101 62-63 0.5402658531152866 1101 64-65 0.4324328759528768 1101 66-67 0.3298980896427679 1101 68-69 0.3090738102112027 1101 70-71 0.35483358786152763 1101 72-73 0.3442841644817989 1101 74-75 0.35397338276457546 1101 76-77 0.2777557889450648 1101 78-79 0.13786384385263872 1101 80-81 0.23853326411964204 1101 82-83 0.10181203540276229 1101 84-85 -0.003950443692364303 1101 86-87 0.05717300161812844 1101 88-89 0.02541836168366629 1101 90-91 0.19998038672253315 1101 92-93 0.1115659652082357 1101 94-95 -0.13231812851705982 1101 96-97 -0.12321292436332243 1101 98-99 -0.25343815857681307 1101 100-101 -0.36598599912432306 1102 1 0.2313082958033199 1102 2 0.2552619849320017 1102 3 0.46081686390332877 1102 4 0.24376223090010285 1102 5 0.3528370899868065 1102 6 0.3100122608107654 1102 7 0.3039550454640221 1102 8 0.4343378491335059 1102 9 0.47848771508650856 1102 10-11 0.444573371113016 1102 12-13 0.4070893765432686 1102 14-15 0.5958985513077053 1102 16-17 0.5461203374950401 1102 18-19 0.4698397339965368 1102 20-21 0.5527214717259028 1102 22-23 0.5116634617076841 1102 24-25 0.4973567925511375 1102 26-27 0.46334277024942594 1102 28-29 0.4282856085094835 1102 30-31 0.41613969545819884 1102 32-33 0.5427074376697831 1102 34-35 0.44208767649026726 1102 36-37 0.38545155689214283 1102 38-39 0.5202494510689561 1102 40-41 0.5644621143143453 1102 42-43 0.5171985420644134 1102 44-45 0.47207914474707735 1102 46-47 0.5091369903418652 1102 48-49 0.5418444030186791 1102 50-51 0.5247803053785631 1102 52-53 0.7087703359961992 1102 54-55 0.7167096016887697 1102 56-57 0.6736403730279221 1102 58-59 0.5889659323331813 1102 60-61 0.6818641184447145 1102 62-63 0.6560325150637141 1102 64-65 0.6991328324402062 1102 66-67 0.7437636276010267 1102 68-69 0.7399669603718877 1102 70-71 0.5745221576574338 1102 72-73 0.502899674916975 1102 74-75 0.30262121315949386 1102 76-77 0.4399316063805401 1102 78-79 0.2893495659271963 1102 80-81 0.2138525973795815 1102 82-83 0.22657088079644794 1102 84-85 0.2146791428323347 1102 86-87 0.23952913454674274 1102 88-89 0.3078264608086947 1102 90-91 0.45367077304173264 1102 92-93 0.19426725066713502 1102 94-95 0.13425207630708158 1102 96-97 -0.08295190071444125 1102 98-99 -0.024078502693953396 1102 100-101 -0.28928474512037283 1103 1 0.29380268422216105 1103 2 0.3493490180882439 1103 3 0.3122069122536679 1103 4 0.34770431492513154 1103 5 0.31001587034585754 1103 6 0.4384383499987692 1103 7 0.4104136649481944 1103 8 0.44532031043509335 1103 9 0.5711837128909139 1103 10-11 0.4697702460752069 1103 12-13 0.40098381705031727 1103 14-15 0.5576048031026133 1103 16-17 0.5146880009058705 1103 18-19 0.5018735047307246 1103 20-21 0.6084964493910192 1103 22-23 0.6749556975717468 1103 24-25 0.5355796995189124 1103 26-27 0.6557496402893506 1103 28-29 0.7553767940765965 1103 30-31 0.7783426637219648 1103 32-33 0.761254246693909 1103 34-35 0.5700506709382296 1103 36-37 0.7429901470591815 1103 38-39 0.9407835717111013 1103 40-41 0.8555845770041017 1103 42-43 0.8064753119332266 1103 44-45 0.8401834268690394 1103 46-47 0.7855443748961193 1103 48-49 0.6146348091093117 1103 50-51 0.5513147265785889 1103 52-53 0.7379220623255165 1103 54-55 0.8343367800170185 1103 56-57 0.7913329800045545 1103 58-59 0.7092151916044713 1103 60-61 0.799124991858271 1103 62-63 0.7523600416442946 1103 64-65 0.7124019533657204 1103 66-67 0.8293078443134405 1103 68-69 0.8507039130079193 1103 70-71 0.8168650954706109 1103 72-73 0.8206724518871198 1103 74-75 0.6973859979645098 1103 76-77 0.6215425902427469 1103 78-79 0.6595058560241327 1103 80-81 0.6069569909371957 1103 82-83 0.6776398034301785 1103 84-85 0.5593335426621913 1103 86-87 0.7315000364687201 1103 88-89 0.7188626022568378 1103 90-91 0.668429565792426 1103 92-93 0.8142366472526206 1103 94-95 0.5968205216778202 1103 96-97 0.27493627901916895 1103 98-99 0.238892925841089 1103 100-101 0.13810203147916056 1104 1 0.15529453918751202 1104 2 0.19258761518003453 1104 3 0.24105886314308478 1104 4 0.20348431060406114 1104 5 0.3225507218534176 1104 6 0.20869758629128654 1104 7 0.22583933268016665 1104 8 0.1851524620205609 1104 9 0.215094601526026 1104 10-11 0.2823797342899965 1104 12-13 0.327353957129759 1104 14-15 0.2509625102779296 1104 16-17 0.37879588181262847 1104 18-19 0.20466040944921104 1104 20-21 0.3507734675516829 1104 22-23 0.4128410282787698 1104 24-25 0.3322049605837165 1104 26-27 0.37157224810338363 1104 28-29 0.4439794508814856 1104 30-31 0.5532900766434636 1104 32-33 0.376390720567791 1104 34-35 0.4178572589838154 1104 36-37 0.40932992739531215 1104 38-39 0.4577465872585478 1104 40-41 0.43087786278692164 1104 42-43 0.3540468598218425 1104 44-45 0.415910222849881 1104 46-47 0.3044439230541016 1104 48-49 0.13395196741488746 1104 50-51 0.17575974467191458 1104 52-53 0.17370035576376353 1104 54-55 0.28283651673089594 1104 56-57 0.20280477284747178 1104 58-59 0.3912581423171133 1104 60-61 0.3015029846920285 1104 62-63 0.22087466828773472 1104 64-65 0.34924199605267603 1104 66-67 0.37038422160855333 1104 68-69 0.4250025103428783 1104 70-71 0.3434883269407365 1104 72-73 0.21200075145154784 1104 74-75 0.328217224541163 1104 76-77 0.4774591392958101 1104 78-79 0.24548163744146478 1104 80-81 0.27792789478024105 1104 82-83 0.16556090925062605 1104 84-85 0.11175928241203437 1104 86-87 0.15222986748859668 1104 88-89 0.22860652726845743 1104 90-91 0.2182168324708762 1104 92-93 0.29148320746377365 1104 94-95 0.31485089825648416 1104 96-97 0.20168100558039015 1104 98-99 -0.045917194821587515 1104 100-101 -0.23425844324022904 1105 1 0.2864435725966068 1105 2 0.2596865593841535 1105 3 0.2902227192420206 1105 4 0.2887367729057928 1105 5 0.24735550959445618 1105 6 0.19659564822153186 1105 7 0.2213338704028729 1105 8 0.30906642198829815 1105 9 0.34183257107413567 1105 10-11 0.3494035263568591 1105 12-13 0.42129718366152247 1105 14-15 0.4270480920654478 1105 16-17 0.49593408231608294 1105 18-19 0.4040804243574314 1105 20-21 0.3778827680869057 1105 22-23 0.4774845444212019 1105 24-25 0.38383004917769625 1105 26-27 0.5014676766538599 1105 28-29 0.3802773221819251 1105 30-31 0.46697799975610366 1105 32-33 0.3468718164448106 1105 34-35 0.39921341901509777 1105 36-37 0.3670394368901455 1105 38-39 0.5093964210684518 1105 40-41 0.41677049298953506 1105 42-43 0.3296002667187352 1105 44-45 0.5559201820355497 1105 46-47 0.5842530488453335 1105 48-49 0.6386381308677329 1105 50-51 0.6655052966429764 1105 52-53 0.6373959162887175 1105 54-55 0.48177568015930206 1105 56-57 0.5655334369724088 1105 58-59 0.5746111801742444 1105 60-61 0.6782004759548244 1105 62-63 0.5862133416997608 1105 64-65 0.3409634993398143 1105 66-67 0.38370615992269563 1105 68-69 0.44612835656816685 1105 70-71 0.2826390772401268 1105 72-73 0.5194405075431519 1105 74-75 0.6302278497091827 1105 76-77 0.5851523047282683 1105 78-79 0.48165975411668427 1105 80-81 0.5131771493687971 1105 82-83 0.4951798634750304 1105 84-85 0.5844168880527221 1105 86-87 0.48421290632331093 1105 88-89 0.2685144506254993 1105 90-91 0.06879987252757758 1105 92-93 0.5009259513110713 1105 94-95 0.4162065389437295 1105 96-97 0.49021892991502014 1105 98-99 0.46330543880257835 1105 100-101 -1.705335694310861E-4 1106 1 0.22232702863216858 1106 2 0.30022517585866737 1106 3 0.26097618037087855 1106 4 0.2684434451287885 1106 5 0.32149833035659725 1106 6 0.30529940337271455 1106 7 0.3115014862754464 1106 8 0.1873973244284528 1106 9 0.30667780420836266 1106 10-11 0.3726052441880441 1106 12-13 0.39587119857100106 1106 14-15 0.49360160644244644 1106 16-17 0.4421907962115341 1106 18-19 0.448129655732167 1106 20-21 0.5234478349943501 1106 22-23 0.5531539082217307 1106 24-25 0.5718610835806288 1106 26-27 0.4570202418249494 1106 28-29 0.44426769121466236 1106 30-31 0.39360583001030847 1106 32-33 0.28367206647953225 1106 34-35 0.35937455250586936 1106 36-37 0.41238832793982994 1106 38-39 0.6318159122014819 1106 40-41 0.610465508500944 1106 42-43 0.6107055706961333 1106 44-45 0.608923492680546 1106 46-47 0.5018943879202098 1106 48-49 0.6275720684516557 1106 50-51 0.6039843099318531 1106 52-53 0.43279429337813013 1106 54-55 0.4802016768255086 1106 56-57 0.5906402226370844 1106 58-59 0.5316419599121645 1106 60-61 0.5230825776356127 1106 62-63 0.5868123832333083 1106 64-65 0.5992855463833919 1106 66-67 0.3516140290711931 1106 68-69 0.25872240898284815 1106 70-71 0.3725714173799659 1106 72-73 0.3898733222859363 1106 74-75 0.44707191369955623 1106 76-77 0.5534589561150369 1106 78-79 0.6270063300300066 1106 80-81 0.5834325667878808 1106 82-83 0.5636028504639619 1106 84-85 0.7345775006378013 1106 86-87 0.6872735165932546 1106 88-89 0.5639530532091932 1106 90-91 0.6466784552010978 1106 92-93 0.770071260844805 1106 94-95 0.6796612433008171 1106 96-97 0.717896095725088 1106 98-99 0.5766145789609354 1106 100-101 0.40998249694406397 1107 1 0.09309685423604108 1107 2 0.12799289188669505 1107 3 0.1263957553354409 1107 4 0.08551744308026343 1107 5 0.10645386646697119 1107 6 0.07147564452911581 1107 7 0.19256990843481248 1107 8 0.10340432469299543 1107 9 0.3582742308175071 1107 10-11 0.3271832272707442 1107 12-13 0.30160503928784976 1107 14-15 0.30590955703360123 1107 16-17 0.2960246620262339 1107 18-19 0.3109258272929125 1107 20-21 0.3820638582743001 1107 22-23 0.4358138391687305 1107 24-25 0.2458171487807661 1107 26-27 0.3253616810847646 1107 28-29 0.27334710894459846 1107 30-31 0.27180410979025993 1107 32-33 0.2741357322575979 1107 34-35 0.2561113235660102 1107 36-37 0.38094140310449376 1107 38-39 0.4409637385243741 1107 40-41 0.43423622270429263 1107 42-43 0.5099317769190392 1107 44-45 0.5771204497360927 1107 46-47 0.5358892530362311 1107 48-49 0.5199319783707281 1107 50-51 0.6251292466106619 1107 52-53 0.6595821758659426 1107 54-55 0.6241929468444241 1107 56-57 0.6609438726781178 1107 58-59 0.4821339116429044 1107 60-61 0.4802076530987378 1107 62-63 0.7017918979630338 1107 64-65 0.6886932371779082 1107 66-67 0.46551925417174544 1107 68-69 0.5735486233456015 1107 70-71 0.43205925170703097 1107 72-73 0.5026872997521394 1107 74-75 0.48731326002950226 1107 76-77 0.4750298778843671 1107 78-79 0.5839236699294794 1107 80-81 0.48004978391140085 1107 82-83 0.4905833758907967 1107 84-85 0.5948520168259144 1107 86-87 0.6618068322902602 1107 88-89 0.693250211725843 1107 90-91 0.6394399306832135 1107 92-93 0.6296835669300158 1107 94-95 0.7011097282726304 1107 96-97 0.5542403228809576 1107 98-99 0.5067594681573269 1107 100-101 0.6063604366142634 1108 1 0.17187297922250977 1108 2 0.12474911118399135 1108 3 0.17170777848982866 1108 4 0.08727797757394029 1108 5 0.09223012599568392 1108 6 0.038941032716302004 1108 7 0.10222561154930077 1108 8 0.15826310455891246 1108 9 0.23902222006725538 1108 10-11 0.2646514375635505 1108 12-13 0.19132551957084587 1108 14-15 0.24724708742866142 1108 16-17 0.2518464033966552 1108 18-19 0.20886322905246146 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66-67 -0.7729184476312909 2316 68-69 -1.0039352672544979 2316 70-71 -0.8341712880914329 2316 72-73 -0.9970410356021304 2316 74-75 -1.0113101818767731 2316 76-77 -0.7318055251642264 2316 78-79 -0.8856316548834116 2316 80-81 -0.7739107823218134 2316 82-83 -0.5963867096388533 2316 84-85 -0.7015876548071383 2316 86-87 -0.6081712626425357 2316 88-89 -0.6988471229640751 2316 90-91 -0.7010905993590164 2316 92-93 -0.5625034321919209 2316 94-95 -0.5340556863440646 2316 96-97 -0.6241011871052464 2316 98-99 -0.6193781783603924 2316 100-101 -0.2726398514269128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7412.0 3 2255.0 4 2255.0 5 2409.0 6 2647.0 7 3250.0 8 3936.0 9 4669.0 10 5480.0 11 6876.0 12 8607.0 13 11751.0 14 11105.0 15 12075.0 16 14787.0 17 19360.0 18 16203.0 19 12617.0 20 12224.0 21 12779.0 22 13723.0 23 15342.0 24 16870.0 25 18859.0 26 21801.0 27 25552.0 28 30195.0 29 35344.0 30 42582.0 31 52064.0 32 65443.0 33 85573.0 34 117161.0 35 172845.0 36 265288.0 37 394258.0 38 335584.0 39 55259.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.597748073635564 18.555088144864055 14.91468791057754 22.932475870922836 2 15.45366753449784 19.717689487386806 40.24305131922056 24.58559165889479 3 19.455007483888313 23.981842199823696 29.333998567850045 27.229151748437946 4 13.082943747653955 16.3036565731144 36.18384928230407 34.429550396927574 5 14.374346559503287 36.77126195841352 33.31618402125343 15.538207460829764 6 32.682170430483666 35.13995860197441 17.1420481378639 15.035822829678022 7 29.202165178859797 32.60464815843528 19.834795302750397 18.358391359954528 8 27.046474468291816 34.21773938495693 19.276579207931025 19.459206938820227 9 26.918920985956035 16.91585660343428 19.849499027717417 36.31572338289227 10-11 25.654997971822546 26.130495779582326 26.388061398228125 21.82644485036701 12-13 26.551760756345054 23.458251395177165 27.12719463131641 22.86279321716137 14-15 23.258224569794518 24.834516478076598 25.66157237507738 26.2456865770515 16-17 22.678119004200582 28.0614910327515 26.377435202089284 22.882954760958636 18-19 22.721065726024303 26.12285004920854 29.03921516444748 22.116869060319676 20-21 23.41497093980963 25.317209942659968 29.063368604865385 22.204450512665012 22-23 23.019248818526496 25.242444978272466 29.11492336443961 22.623382838761426 24-25 22.76291360332123 25.555485490286888 29.12828008832359 22.553320818068293 26-27 22.447509651422013 25.867490847366163 28.957806655229856 22.727192845981964 28-29 22.457665225013645 26.099045077154624 29.028290028612542 22.414999669219192 30-31 22.573304632527176 25.72180546331732 28.88079776526496 22.82409213889054 32-33 22.27005632170216 25.819638773165167 28.83290280921188 23.07740209592079 34-35 23.02633264718694 25.935971231028415 28.492649485312562 22.545046636472087 36-37 23.105017221992007 25.495182481070838 28.60022043527804 22.79957986165911 38-39 22.66785981600663 25.74378603315757 28.601800139022277 22.986554011813524 40-41 22.900398138050548 25.719148186619762 28.71277331516629 22.667680360163402 42-43 22.76466262856989 25.700297493972137 28.372354047363775 23.1626858300942 44-45 22.765492332545055 25.992928807645843 28.413609816747076 22.827969043062026 46-47 23.07903213685048 26.371057398524417 27.68299035194788 22.866920112677224 48-49 23.02097190916449 26.303354691667398 27.576742663563863 23.09893073560425 50-51 23.054416347580453 26.59327359780235 27.4959927954544 22.856317259162793 52-53 23.33793683961735 26.33561504493926 27.45836435668095 22.86808375876245 54-55 22.934538158542207 26.46039321104212 27.475383821666384 23.12968480874929 56-57 22.90465540597079 26.698605448107077 27.387163988017903 23.009575157904226 58-59 22.96560307615398 26.224678908944444 27.478944689580683 23.330773325320898 60-61 22.752230584188972 26.415693743334657 27.830228859322965 23.001846813153414 62-63 22.735111650958356 26.48621800385954 27.916418795251648 22.862251549930455 64-65 22.496827720333485 26.423534583031827 27.772349539604775 23.30728815702992 66-67 22.459098312303215 26.617328568510914 27.56091062433066 23.362662494855215 68-69 22.784247071579973 26.360122111142708 27.75311920248785 23.102511614789478 70-71 22.741276280935157 26.41729571902233 27.45682952428426 23.384598475758253 72-73 22.728322387066324 26.453733458463148 27.457882076812805 23.36006207765772 74-75 22.726800306769377 26.912273962988102 27.12081286109096 23.24011286915156 76-77 22.869484048891202 26.541092251741063 27.256278677555564 23.333145021812175 78-79 22.87134156262598 26.738110758637703 27.26431636797395 23.126231310762368 80-81 23.018089802085548 26.903784368046107 27.052937212533607 23.025188617334738 82-83 23.064973764332446 26.82013344561767 27.01776251862408 23.097130271425794 84-85 22.877082362648153 26.901743942276646 26.95017670573248 23.27099698934272 86-87 23.04054253646994 26.78341088814642 26.946734639197807 23.229311936185837 88-89 22.847548763497038 26.79333358212669 26.878856296959746 23.48026135741653 90-91 22.77993358892008 26.89060316917199 27.021403446014432 23.308059795893506 92-93 22.96032232762683 26.470643125939297 27.090482013967048 23.478552532466825 94-95 22.741554871948004 26.41774262279275 27.257278709702838 23.583423795556413 96-97 22.95988633827428 26.164852532413214 27.245219574141803 23.630041555170703 98-99 22.731019188810492 26.344193877640944 26.97656359750411 23.948223336044453 100-101 22.899196084360998 25.771141493868726 27.513412198589943 23.816250223180333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2136.0 1 1270.5 2 557.5 3 718.0 4 610.0 5 554.5 6 949.5 7 2095.0 8 3364.0 9 3745.5 10 3119.5 11 2482.5 12 2457.5 13 2585.5 14 2721.0 15 2878.0 16 3036.0 17 3193.0 18 3345.0 19 3535.5 20 3765.5 21 4225.5 22 4952.5 23 5922.5 24 7108.5 25 8559.5 26 10866.5 27 13581.5 28 15749.0 29 17982.5 30 21004.5 31 24458.0 32 28629.5 33 30763.5 34 31260.0 35 32818.5 36 35515.0 37 39087.5 38 42000.0 39 43894.0 40 46098.0 41 50121.0 42 53960.5 43 56630.0 44 59472.0 45 63658.0 46 65884.0 47 67893.5 48 72811.0 49 74014.5 50 72285.0 51 71201.0 52 70966.5 53 72378.0 54 73769.5 55 70780.0 56 64724.0 57 59565.0 58 54880.5 59 48527.5 60 40291.0 61 33916.5 62 29198.5 63 22617.5 64 16625.0 65 13084.0 66 10303.0 67 8475.5 68 6524.5 69 4656.0 70 3507.0 71 2579.0 72 2004.0 73 1699.0 74 1357.0 75 1058.0 76 775.0 77 514.5 78 356.5 79 254.5 80 176.5 81 125.5 82 87.0 83 61.5 84 48.0 85 34.5 86 16.5 87 12.5 88 11.0 89 5.5 90 5.0 91 5.5 92 9.5 93 8.0 94 4.5 95 4.0 96 3.0 97 2.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28402210414935636 2 0.11076862061824898 3 0.11949584527302012 4 0.12202622401907802 5 0.12780994686721037 6 0.057372465038169985 7 0.06682265504895767 8 0.0715219298630652 9 0.06878498958671686 10-11 0.06372423209460105 12-13 0.06819112518713184 14-15 0.06356931094688323 16-17 0.06506688204148892 18-19 0.06906901169086621 20-21 0.0740264884178368 22-23 0.08246969096845856 24-25 0.09114527524065709 26-27 0.09191988097924625 28-29 0.08536155239252474 30-31 0.08076555834356243 32-33 0.0735617249746833 34-35 0.24092820489251313 36-37 0.3668790979871095 38-39 0.33889001063275476 40-41 0.32192614495765226 42-43 0.3450610363501817 44-45 0.35890065887964123 46-47 0.36478766249291883 48-49 0.37416039192984757 50-51 0.36827338831656997 52-53 0.36884143252486873 54-55 0.3560346176468614 56-57 0.33166035707258934 58-59 0.3168912076568228 60-61 0.2739264093564111 62-63 0.3462487651493517 64-65 0.3747542563294326 66-67 0.37857564463980575 68-69 0.3909435162659459 70-71 0.39120171817880894 72-73 0.37555468225930805 74-75 0.3786531052136647 76-77 0.36365157407632137 78-79 0.3416011307178168 80-81 0.33925149331076304 82-83 0.3534267783269446 84-85 0.3609662741825457 86-87 0.354562866743542 88-89 0.3695902180721716 90-91 0.39112425760495 92-93 0.38730286929457686 94-95 0.38663154432113295 96-97 0.3918214027696803 98-99 0.3808994618555732 100-101 0.36021748863524283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1936469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.83456118951331 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21780835898385 41.79370076191299 2 13.21705564879527 14.495028934381335 3 4.474499085210552 7.360715816411981 4 2.0321402213109616 4.457260692445882 5 1.0786808029108101 2.9574494245583076 6 0.6574066534591819 2.162916321930041 7 0.45348508572517054 1.7406658977209986 8 0.31908984330963874 1.3997721230331681 9 0.21469551218515562 1.0595460780027732 >10 1.208551405945922 11.960144452557632 >50 0.08048164775283771 3.0156820916926166 >100 0.04024948567343803 3.903871097543251 >500 0.004250503115770935 1.6522390432657488 >1k 0.0014168343719236443 1.2632636446787333 >5k 1.8891124958981924E-4 0.777743619864529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8500 0.43894325186718713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6516 0.3364887328431284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4548 0.23486045994023141 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.164038257261025E-5 0.0 5 0.0 0.0 0.0 5.164038257261025E-5 0.0 6 0.0 0.0 0.0 1.032807651452205E-4 5.164038257261025E-5 7 0.0 0.0 0.0 1.032807651452205E-4 5.164038257261025E-5 8 0.0 0.0 0.0 1.032807651452205E-4 5.164038257261025E-5 9 0.0 0.0 0.0 1.032807651452205E-4 5.164038257261025E-5 10-11 0.0 0.0 0.0 1.5492114771783075E-4 5.164038257261025E-5 12-13 0.0 0.0 0.0 1.5492114771783075E-4 1.8074133900413588E-4 14-15 0.0 0.0 0.0 1.5492114771783075E-4 2.06561530290441E-4 16-17 0.0 0.0 0.0 1.8074133900413588E-4 2.06561530290441E-4 18-19 0.0 0.0 0.0 2.06561530290441E-4 2.3238172157674615E-4 20-21 0.0 0.0 0.0 2.5820191286305127E-4 3.098422954356615E-4 22-23 0.0 0.0 0.0 4.13123060580882E-4 3.6148267800827175E-4 24-25 0.0 0.0 0.0 5.680442082987128E-4 3.6148267800827175E-4 26-27 0.0 0.0 0.0 8.26246121161764E-4 3.6148267800827175E-4 28-29 0.0 0.0 0.0 0.0015492114771783077 3.6148267800827175E-4 30-31 0.0 0.0 0.0 0.004957476726970584 3.6148267800827175E-4 32-33 0.0 0.0 0.0 0.01179982741784144 3.6148267800827175E-4 34-35 0.0 0.0 0.0 0.021585679915351083 4.13123060580882E-4 36-37 0.0 0.0 0.0 0.038394624442735725 4.13123060580882E-4 38-39 0.0 0.0 0.0 0.06274306482572145 4.13123060580882E-4 40-41 0.0 0.0 0.0 0.09197152136181885 4.13123060580882E-4 42-43 0.0 0.0 0.0 0.12321395281824807 4.13123060580882E-4 44-45 0.0 0.0 0.0 0.1606532301833905 4.13123060580882E-4 46-47 0.0 0.0 0.0 0.20777507928089733 4.13123060580882E-4 48-49 0.0 0.0 0.0 0.25812445228919234 4.13123060580882E-4 50-51 0.0 0.0 0.0 0.31474813178005945 4.6476344315349224E-4 52-53 0.0 0.0 0.0 0.3727919217916734 5.164038257261025E-4 54-55 0.0 0.0 0.0 0.4407764854485148 5.680442082987128E-4 56-57 0.0 0.0 0.0 0.5230912552692555 5.938643995850179E-4 58-59 0.0 0.0 0.0 0.6148562151007839 6.19684590871323E-4 60-61 0.0 0.0 0.0 0.7168201504904029 6.713249734439333E-4 62-63 0.0 0.0 0.0 0.8209013415654989 7.229653560165435E-4 64-65 0.0 0.0 0.0 0.9330900727044946 7.746057385891537E-4 66-67 0.0 0.0 0.0 1.0712022758949407 7.746057385891537E-4 68-69 0.0 0.0 0.0 1.229299307140987 7.746057385891537E-4 70-71 0.0 0.0 0.0 1.4030950146891068 7.746057385891537E-4 72-73 0.0 0.0 0.0 1.5884581679334913 8.004259298754589E-4 74-75 0.0 0.0 0.0 1.782135422772066 8.26246121161764E-4 76-77 0.0 0.0 0.0 1.9979664017342906 8.26246121161764E-4 78-79 0.0 0.0 0.0 2.2480091341508697 8.778865037343742E-4 80-81 0.0 0.0 0.0 2.521290038725123 8.778865037343742E-4 82-83 0.0 0.0 0.0 2.8131098406429436 9.811672688795948E-4 84-85 0.0 0.0 0.0 3.1140441700848296 0.001032807651452205 86-87 0.0 0.0 0.0 3.435892854468623 0.0010844480340248153 88-89 0.0 0.0 0.0 3.7897069356648623 0.0011360884165974256 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4905 0.0 45.80052 1 GTATCAA 8325 0.0 38.33834 1 ATCAACG 10765 0.0 29.603334 3 TATCAAC 10805 0.0 29.537632 2 TCAACGC 10975 0.0 28.993683 4 CAACGCA 11220 0.0 28.318312 5 AACGCAG 11475 0.0 27.71601 6 ACGCAGA 12960 0.0 24.357355 7 CGCAGAG 13145 0.0 24.050613 8 GTACATG 8690 0.0 23.665539 1 TACATGG 8770 0.0 22.710938 2 GAGTACT 7590 0.0 22.543758 12-13 GCAGAGT 14100 0.0 22.152727 9 ACATGGG 8865 0.0 21.611654 3 AGTACTT 7885 0.0 21.610165 12-13 GTACTTT 8020 0.0 21.247227 14-15 AGAGTAC 12405 0.0 20.537264 10-11 TATAGGG 330 2.910383E-11 20.118681 2 TACTTTT 8755 0.0 19.219849 14-15 CATGGGG 4600 0.0 19.072134 4 >>END_MODULE