FastQCFastQC Report
Fri 27 May 2016
SRR939236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936469
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86700.4477221169045309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70430.363703214458894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.26212658193856964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51500.046.1017951
GTATCAA86150.037.997731
TATCAAC112650.029.0675432
ATCAACG113100.028.7840543
TCAACGC114650.028.3949094
CAACGCA117550.027.5732845
AACGCAG120000.027.010336
GTAAGGT9550.024.3491124
GTACATG84800.024.2388731
ACGCAGA135850.023.8240287
TACCGTG1801.9700565E-723.7279627
CGCAGAG137800.023.4180168
TACATGG86050.023.3281292
ACATGGG85750.022.579613
GAGTACT82400.021.82798612-13
TAAGGTG11350.021.7419225
GCAGAGT148850.021.2975319
CATGGGG45300.021.266124
GGTAAGG10500.020.7902163
GTACTTT85350.020.73991614-15
Produced by FastQC (version 0.11.5)