##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939236_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936469 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22913405791675 33.0 31.0 34.0 31.0 34.0 2 32.440042675612155 34.0 31.0 34.0 31.0 34.0 3 32.519107716157606 34.0 31.0 34.0 30.0 34.0 4 36.0737667372935 37.0 35.0 37.0 35.0 37.0 5 36.00657330429767 37.0 35.0 37.0 35.0 37.0 6 36.04778181318679 37.0 35.0 37.0 35.0 37.0 7 35.994106283136986 37.0 35.0 37.0 35.0 37.0 8 35.96841157798033 37.0 35.0 37.0 35.0 37.0 9 37.66344981510161 39.0 37.0 39.0 35.0 39.0 10-11 37.57519304466015 39.0 37.0 39.0 35.0 39.0 12-13 37.49110597690952 39.0 37.0 39.0 35.0 39.0 14-15 38.76146455223399 40.0 38.0 41.0 34.5 41.0 16-17 38.69859806689392 40.0 38.0 41.0 34.0 41.0 18-19 38.81990958801819 40.0 38.0 41.0 34.5 41.0 20-21 38.82122667597571 40.0 38.0 41.0 34.0 41.0 22-23 38.762930880897144 40.0 38.0 41.0 34.0 41.0 24-25 38.6857047543751 40.0 38.0 41.0 34.0 41.0 26-27 38.50188203374286 40.0 38.0 41.0 34.0 41.0 28-29 38.335605682301136 40.0 38.0 41.0 34.0 41.0 30-31 38.11502817757475 40.0 37.5 41.0 33.0 41.0 32-33 37.88072620837204 40.0 37.0 41.0 33.0 41.0 34-35 37.82025583678334 40.0 37.0 41.0 33.0 41.0 36-37 37.71714574310252 40.0 37.0 41.0 33.0 41.0 38-39 37.587016884855885 40.0 37.0 41.0 33.0 41.0 40-41 37.38781669110118 40.0 36.5 41.0 32.0 41.0 42-43 37.18375326431769 40.0 36.0 41.0 31.0 41.0 44-45 36.92818733478305 40.0 36.0 41.0 31.0 41.0 46-47 36.59478927883689 39.5 35.5 41.0 30.0 41.0 48-49 36.22930705319837 39.0 35.0 41.0 29.5 41.0 50-51 35.94363116579713 39.0 35.0 41.0 29.0 41.0 52-53 35.67819520994139 39.0 35.0 40.0 28.0 41.0 54-55 35.36149636270966 38.0 35.0 40.0 27.5 41.0 56-57 35.07857187489188 38.0 34.0 40.0 27.0 41.0 58-59 34.74855832961953 38.0 34.0 40.0 26.0 41.0 60-61 34.385678521060754 37.0 34.0 40.0 26.0 41.0 62-63 33.96804544766789 36.5 33.0 39.5 25.5 41.0 64-65 33.51963367345411 36.0 33.0 39.0 24.5 40.5 66-67 33.128973404686576 35.5 32.5 39.0 23.0 40.0 68-69 32.71700373205044 35.0 32.0 38.0 22.5 40.0 70-71 32.2976525831294 35.0 32.0 37.5 21.5 40.0 72-73 31.79618005762034 35.0 31.0 37.0 20.0 39.0 74-75 31.294383230508725 35.0 31.0 36.5 18.5 39.0 76-77 29.35700571504114 32.5 28.5 35.0 13.0 37.0 78-79 30.34041804955308 34.0 30.0 35.0 14.0 37.0 80-81 30.405008549065336 34.0 30.0 35.0 14.0 37.0 82-83 30.09670332961695 34.0 30.0 35.0 9.5 37.0 84-85 29.71476925269653 34.0 30.0 35.0 6.5 36.0 86-87 29.171231246149564 34.0 29.0 35.0 2.0 36.0 88-89 28.64815083536065 34.0 29.0 35.0 2.0 35.0 90-91 28.215328259837882 33.5 28.5 35.0 2.0 35.0 92-93 27.84200392570188 33.0 27.0 35.0 2.0 35.0 94-95 27.397428257307503 33.0 27.0 35.0 2.0 35.0 96-97 26.79509974081692 33.0 25.5 35.0 2.0 35.0 98-99 25.976214181585142 33.0 24.5 35.0 2.0 35.0 100-101 22.572347401378487 28.5 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11094164791865069 1101 2 0.14198191471575683 1101 3 0.16358859826059557 1101 4 0.15630729531119414 1101 5 0.14398709591985437 1101 6 0.07075919308628187 1101 7 0.13844516402452456 1101 8 0.16053413326056187 1101 9 0.27012025696424047 1101 10-11 0.2089557044845165 1101 12-13 0.2903478126518664 1101 14-15 0.2696448963251825 1101 16-17 0.214396333225217 1101 18-19 0.27031461756519803 1101 20-21 0.23317357791555082 1101 22-23 0.23482402595727336 1101 24-25 0.25007908787820554 1101 26-27 0.27280428568170123 1101 28-29 0.17364065489906721 1101 30-31 0.14149083745226676 1101 32-33 0.10810270707268899 1101 34-35 0.20452609613342076 1101 36-37 0.3001494406455194 1101 38-39 0.3267679943804964 1101 40-41 0.3572294353647152 1101 42-43 0.5077976888032296 1101 44-45 0.5850051571360595 1101 46-47 0.5923047177382017 1101 48-49 0.5326342163154152 1101 50-51 0.4845679982331603 1101 52-53 0.5532913319806383 1101 54-55 0.5867527630642968 1101 56-57 0.5581866680348497 1101 58-59 0.6310269599539424 1101 60-61 0.5486026258033334 1101 62-63 0.5986095350702456 1101 64-65 0.5891855273993727 1101 66-67 0.5053380631995452 1101 68-69 0.46903920923448084 1101 70-71 0.5395919262992948 1101 72-73 0.34303232837511644 1101 74-75 0.4996901465278576 1101 76-77 0.5122141195976404 1101 78-79 0.8046208554624421 1101 80-81 0.7712443344682143 1101 82-83 0.7902829676755552 1101 84-85 0.4930677854200489 1101 86-87 0.6984081257993964 1101 88-89 0.8810164964696092 1101 90-91 0.8241619552796635 1101 92-93 0.8695991384532498 1101 94-95 0.9744578179990917 1101 96-97 0.8319871339061997 1101 98-99 0.7123825124520842 1101 100-101 0.7058405098976444 1102 1 0.13192996240523058 1102 2 0.06563551185125505 1102 3 0.19286398109353087 1102 4 0.059566290115888876 1102 5 0.11961671914199457 1102 6 0.13181455903839634 1102 7 0.17774877767387665 1102 8 0.19406772976696374 1102 9 0.2744177893235147 1102 10-11 0.26206509168968495 1102 12-13 0.2992567715454939 1102 14-15 0.3583684938276619 1102 16-17 0.3673112895290487 1102 18-19 0.28417150541257286 1102 20-21 0.23596127273963674 1102 22-23 0.15173381316454027 1102 24-25 0.31588546169929543 1102 26-27 0.28714872523315194 1102 28-29 0.17441621597671286 1102 30-31 0.2802769516651864 1102 32-33 0.2715583477313501 1102 34-35 0.3730970978162631 1102 36-37 0.38943234645367397 1102 38-39 0.3531763897062916 1102 40-41 0.4501011542203841 1102 42-43 0.2504827749635794 1102 44-45 0.30923143093041716 1102 46-47 0.2318228148186563 1102 48-49 0.1677856456807021 1102 50-51 0.05319384948271022 1102 52-53 0.15263523417671365 1102 54-55 0.22726300165007984 1102 56-57 0.20699151104844304 1102 58-59 0.1024167182226634 1102 60-61 -0.10543356835757578 1102 62-63 -0.11894566836462417 1102 64-65 0.0294732222234515 1102 66-67 0.012373159058498118 1102 68-69 0.18437978769098606 1102 70-71 -0.03583933415291796 1102 72-73 0.25181101135641626 1102 74-75 0.11719174548774802 1102 76-77 0.20478328116598377 1102 78-79 0.0983232470870341 1102 80-81 0.0766440351219515 1102 82-83 0.10149724664949389 1102 84-85 0.025482110003661518 1102 86-87 0.15772147737660092 1102 88-89 0.19166065449927316 1102 90-91 0.28897861224772114 1102 92-93 0.14635648711562865 1102 94-95 0.014787977003855701 1102 96-97 -1.6270698418452412E-4 1102 98-99 0.227381608122311 1102 100-101 0.04050936792573623 1103 1 0.17241453557165443 1103 2 0.21595679676165958 1103 3 0.19315689767025646 1103 4 0.10215667875887391 1103 5 0.18176374670735385 1103 6 0.09021117190393113 1103 7 0.13984218877019572 1103 8 0.11826060132368355 1103 9 0.2072360989019515 1103 10-11 0.20652396970458398 1103 12-13 0.16893961930592383 1103 14-15 0.3035671453925701 1103 16-17 0.20145842152553683 1103 18-19 0.22078197030301538 1103 20-21 0.2598699515394145 1103 22-23 0.24629938285862352 1103 24-25 0.2608184188258633 1103 26-27 0.2529823944653913 1103 28-29 0.23920347257736552 1103 30-31 0.2612337127210367 1103 32-33 0.29183947759974416 1103 34-35 0.2936450683182912 1103 36-37 0.40874223499891826 1103 38-39 0.35091249479457076 1103 40-41 0.43893164021355346 1103 42-43 0.3780068721278269 1103 44-45 0.5272841024251065 1103 46-47 0.5105483793675987 1103 48-49 0.5018730800053035 1103 50-51 0.6344926861581612 1103 52-53 0.3868791878373159 1103 54-55 0.3805292125130819 1103 56-57 0.43962760741290197 1103 58-59 0.29230211779589865 1103 60-61 0.06174092650460494 1103 62-63 0.28568881313800887 1103 64-65 0.3944800707403786 1103 66-67 0.37863190755332 1103 68-69 0.3895446073912794 1103 70-71 0.40683896130183683 1103 72-73 0.5481784319012064 1103 74-75 0.5966076698337623 1103 76-77 0.6263722198719179 1103 78-79 0.7168167580296974 1103 80-81 0.8617978449796126 1103 82-83 0.8454189092949242 1103 84-85 0.7503748611461951 1103 86-87 0.7646689809950757 1103 88-89 0.9671092148461362 1103 90-91 1.1524347540397208 1103 92-93 1.0286021129478122 1103 94-95 1.0769529934676783 1103 96-97 0.9128721125187091 1103 98-99 0.6794346667763129 1103 100-101 0.3828704219924042 1104 1 0.1162162750371678 1104 2 0.1289339502598139 1104 3 0.18163590820076791 1104 4 0.15648247453301423 1104 5 0.13376991218572698 1104 6 0.03159797430090805 1104 7 0.11654487372753408 1104 8 0.1628826898607727 1104 9 0.17758905196220098 1104 10-11 0.18301714811530445 1104 12-13 0.24838468884058784 1104 14-15 0.2055625081896224 1104 16-17 0.17514944987532743 1104 18-19 0.1204694875475596 1104 20-21 0.15992363759198724 1104 22-23 0.19171357449538817 1104 24-25 0.08481000489329915 1104 26-27 0.25449757573998966 1104 28-29 0.16130351382396668 1104 30-31 0.29384237787439815 1104 32-33 0.3750238648725954 1104 34-35 0.28897980646784305 1104 36-37 0.21952368506480724 1104 38-39 0.2994159726967709 1104 40-41 0.3341755610163446 1104 42-43 0.533601491924891 1104 44-45 0.5632930401240301 1104 46-47 0.6254641231298734 1104 48-49 0.5453260471885386 1104 50-51 0.32898911753213866 1104 52-53 0.32484607644997965 1104 54-55 0.34061681243787945 1104 56-57 0.3192104462079044 1104 58-59 0.4535482702175244 1104 60-61 0.46759859860627984 1104 62-63 0.5217400262650855 1104 64-65 0.42645413682285493 1104 66-67 0.43803122235468095 1104 68-69 0.4037031346350801 1104 70-71 0.2376151924100114 1104 72-73 0.28046928310573094 1104 74-75 0.1811926644885382 1104 76-77 0.47051665218867456 1104 78-79 0.6099048190559628 1104 80-81 0.6872550415762539 1104 82-83 0.48552454174749116 1104 84-85 0.4401405598739139 1104 86-87 0.4569047993192292 1104 88-89 0.783077354886295 1104 90-91 0.8272992996396624 1104 92-93 0.8912121155759998 1104 94-95 1.0354533942424808 1104 96-97 0.8527198835654666 1104 98-99 0.8359931102174905 1104 100-101 0.8084647331414274 1105 1 0.09879900348744286 1105 2 0.1056557284938009 1105 3 0.1453386478933112 1105 4 0.06891648729055078 1105 5 0.1523502088839166 1105 6 0.07784922683653406 1105 7 0.139581753264153 1105 8 0.20213379575807977 1105 9 0.21605533736924087 1105 10-11 0.19317001856035887 1105 12-13 0.1318854342520268 1105 14-15 0.26970073313280807 1105 16-17 0.30432833640171 1105 18-19 0.16649910237757837 1105 20-21 0.18614409608112226 1105 22-23 0.22326952923302912 1105 24-25 0.24448920302610588 1105 26-27 0.1041659592969495 1105 28-29 0.23799443691216737 1105 30-31 0.29810191547490206 1105 32-33 0.30233662607209766 1105 34-35 0.2573923855914373 1105 36-37 0.2755998575603513 1105 38-39 0.2859363981922911 1105 40-41 0.2898455759245735 1105 42-43 0.2986317666273308 1105 44-45 0.4943534795501918 1105 46-47 0.21895872806180705 1105 48-49 0.25909217740336743 1105 50-51 0.3304881888227982 1105 52-53 0.11579009116268679 1105 54-55 0.21856485359436562 1105 56-57 0.16029605854507167 1105 58-59 0.12607237389080694 1105 60-61 0.21350112689525957 1105 62-63 0.2728129299481523 1105 64-65 0.18644952382580726 1105 66-67 0.18063958196070473 1105 68-69 0.44529739863293116 1105 70-71 0.24194575794511763 1105 72-73 0.2451987965109126 1105 74-75 0.21623769467159448 1105 76-77 0.25525313091390345 1105 78-79 0.2946533648926568 1105 80-81 0.4211599588478059 1105 82-83 0.3561033965022382 1105 84-85 0.3353511386064696 1105 86-87 0.4284135853983635 1105 88-89 0.5841954720503111 1105 90-91 0.6596220505744803 1105 92-93 0.6236863927712939 1105 94-95 0.6342702063254748 1105 96-97 0.4558046344025293 1105 98-99 0.5617683075682258 1105 100-101 0.5290797892305825 1106 1 0.10940985221643729 1106 2 0.1320471254470803 1106 3 0.1240937990717157 1106 4 0.11785557605287522 1106 5 0.08888074553252778 1106 6 0.1511018776676636 1106 7 0.12274608164309342 1106 8 0.08607427360222175 1106 9 0.18198000283203442 1106 10-11 0.20788618284242943 1106 12-13 0.20885445772751154 1106 14-15 0.21026684185161315 1106 16-17 0.21324566288223679 1106 18-19 0.10053421142050212 1106 20-21 0.20107151655618338 1106 22-23 0.1987557078689548 1106 24-25 0.17106031243945097 1106 26-27 0.2407760787579889 1106 28-29 0.22959222827208237 1106 30-31 0.3276760461411925 1106 32-33 0.3833846304245512 1106 34-35 0.27950020149179267 1106 36-37 0.2688989847539389 1106 38-39 0.3428792027484846 1106 40-41 0.2986245730075012 1106 42-43 0.34568720256751106 1106 44-45 0.43879252200979124 1106 46-47 0.4052916099435464 1106 48-49 0.0859005882937609 1106 50-51 0.22030679788887397 1106 52-53 0.42138548635646345 1106 54-55 0.42270200227242327 1106 56-57 0.494476731786726 1106 58-59 0.47238580862711643 1106 60-61 0.34688336748422444 1106 62-63 0.2661615431814752 1106 64-65 0.3337740943680174 1106 66-67 0.3503248970507684 1106 68-69 0.31262098524218374 1106 70-71 0.390047635520709 1106 72-73 0.5804843685811107 1106 74-75 0.4926794458407393 1106 76-77 0.546644875137634 1106 78-79 0.6595023601234757 1106 80-81 0.625462060991623 1106 82-83 0.6051900752076129 1106 84-85 0.5207203449777253 1106 86-87 0.7162672224775868 1106 88-89 0.8652599717493459 1106 90-91 0.8393622922747177 1106 92-93 0.8129418449174288 1106 94-95 0.7398386880590522 1106 96-97 0.6203914404404038 1106 98-99 0.6135443065495672 1106 100-101 0.7021058460903333 1107 1 0.03078575692601504 1107 2 0.04046372258592612 1107 3 0.07528187691574573 1107 4 0.01580908398582892 1107 5 0.0673322083300576 1107 6 0.03417134444015346 1107 7 0.06659643063884602 1107 8 0.10868438192996166 1107 9 0.04654319853420219 1107 10-11 0.1296996040860634 1107 12-13 0.17789761924233716 1107 14-15 0.13411098421745038 1107 16-17 0.07677018076614672 1107 18-19 0.19704985009289544 1107 20-21 0.18816223510078345 1107 22-23 0.15393739593662303 1107 24-25 0.15232002089742025 1107 26-27 0.2500114083876923 1107 28-29 0.14888823364437798 1107 30-31 0.17837861629647023 1107 32-33 0.18975643129687114 1107 34-35 0.21190983228210314 1107 36-37 0.2855492021555648 1107 38-39 0.18826204667377766 1107 40-41 0.20052590455006225 1107 42-43 0.1798794588640149 1107 44-45 0.12139123455803968 1107 46-47 0.2624560279787289 1107 48-49 0.35231254572239123 1107 50-51 0.23164322436235096 1107 52-53 0.20310373927493686 1107 54-55 0.19851039038011464 1107 56-57 0.21679702780566856 1107 58-59 0.33674635616718973 1107 60-61 0.2852639119040248 1107 62-63 0.26211875474276525 1107 64-65 0.26484828224915447 1107 66-67 0.2982426235927562 1107 68-69 0.35490577119823996 1107 70-71 0.30232896204370263 1107 72-73 0.14901929868020858 1107 74-75 0.3335124569719703 1107 76-77 0.48051967757687564 1107 78-79 0.7111971431627566 1107 80-81 0.4856907905657053 1107 82-83 0.5272332774216473 1107 84-85 0.42881728371862593 1107 86-87 0.5800774827490507 1107 88-89 0.7958381379784925 1107 90-91 0.7687584855417136 1107 92-93 0.7403692137811717 1107 94-95 0.7251649261630106 1107 96-97 0.8630308182851074 1107 98-99 1.121779961772969 1107 100-101 0.961355590024457 1108 1 -0.006685898960867576 1108 2 -0.019010512574233474 1108 3 -0.007589582736351019 1108 4 0.012660467309814294 1108 5 -0.00245009575510835 1108 6 0.08432196612376686 1108 7 0.01854598057862944 1108 8 0.0488631262242194 1108 9 0.06646693605212306 1108 10-11 0.02656887168402733 1108 12-13 0.07264210383068104 1108 14-15 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-0.7960706975348657 2316 54-55 -0.7719688729777872 2316 56-57 -0.6536607486093402 2316 58-59 -0.6660317313322892 2316 60-61 -0.6110713448010614 2316 62-63 -0.7004293876239132 2316 64-65 -0.7709659218977194 2316 66-67 -0.7043113028266532 2316 68-69 -0.5658278511054391 2316 70-71 -0.5598886298391079 2316 72-73 -0.6816409428097998 2316 74-75 -0.47751289994357293 2316 76-77 -0.46298371619864653 2316 78-79 -0.5101957217784481 2316 80-81 -0.6271953356056237 2316 82-83 -0.604863685602222 2316 84-85 -0.7239670756452661 2316 86-87 -0.9137589005687197 2316 88-89 -1.1422767950983896 2316 90-91 -1.366812161203658 2316 92-93 -1.1068482851851336 2316 94-95 -0.9532440644384863 2316 96-97 -0.9124339965555173 2316 98-99 -0.8839391655551658 2316 100-101 -0.2239997227092907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 24.0 5 50.0 6 125.0 7 305.0 8 671.0 9 1342.0 10 2122.0 11 3191.0 12 4545.0 13 6075.0 14 7891.0 15 10594.0 16 13560.0 17 18584.0 18 17772.0 19 12939.0 20 12035.0 21 12171.0 22 13252.0 23 14110.0 24 16260.0 25 19113.0 26 22747.0 27 27104.0 28 32781.0 29 40001.0 30 48415.0 31 59146.0 32 72787.0 33 92548.0 34 126801.0 35 182985.0 36 282065.0 37 426689.0 38 311895.0 39 23759.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63242383913915 19.268400039355605 15.486740786387104 22.612435335118146 2 16.01786550675482 20.476186295778554 39.057945156880905 24.448003040585725 3 19.481954010108087 24.13588856831687 29.73711430443761 26.64504311713743 4 13.655679486735908 16.9696493979506 35.708446662456254 33.666224452857236 5 14.320187929680259 36.98200177746197 33.34006379652863 15.357746496329144 6 32.67410942287225 35.592100880520164 16.75043597392987 14.98335372267772 7 29.069455798156334 32.75136343520087 19.67663825240683 18.502542514235962 8 27.07830592692163 34.59487345266049 19.015641355477417 19.311179264940467 9 26.67654375050672 16.88377144173235 19.702096961015126 36.737587846745804 10-11 25.612880970467383 26.432568763042426 26.212709834239533 21.741840432250658 12-13 26.530298187061092 23.597072816554252 27.110916828516235 22.761712167868424 14-15 23.10649584310866 25.042126642784147 25.60618212033218 26.245195393775017 16-17 22.504693485872806 28.223813498906896 26.37841050721148 22.893082508008813 18-19 22.609140657557646 26.20829458152958 29.150247176691185 22.03231758422159 20-21 23.293633928557597 25.319202114776946 29.29556321325051 22.091600743414947 22-23 22.881569495819456 25.246879758984008 29.369460600711918 22.502090144484626 24-25 22.74006664196533 25.591754057323453 29.25840480152649 22.409774499184724 26-27 22.36938762283782 25.899282990406906 29.16178121201965 22.56954817473562 28-29 22.38089016658671 26.154020539445767 29.216372686575408 22.24871660739211 30-31 22.554221110691678 25.72336556898148 29.027472167124802 22.694941153202038 32-33 22.266171056701655 25.82804062445616 29.021946646189534 22.883841672652647 34-35 22.96700828085179 25.924927507042728 28.71534475145825 22.392719460647236 36-37 23.11113601845393 25.526551413963738 28.78899437492914 22.57331819265319 38-39 22.656674648196823 25.798236936538444 28.778516024128958 22.766572391135774 40-41 22.843825432311803 25.687175690287567 28.92838115592872 22.540617721471914 42-43 22.75731376265805 25.752588675749145 28.510356897829002 22.979740663763806 44-45 22.80170444994876 25.981371683026843 28.531927193970645 22.68499667305375 46-47 23.11601488690486 26.338145705555533 27.831483831121318 22.714355576418292 48-49 23.03643900315471 26.356011895878527 27.632587973264737 22.974961127702016 50-51 23.06830111920201 26.592628128826227 27.604779627249393 22.73429112472237 52-53 23.41170966330987 26.39081751373247 27.419054991326995 22.778417831630666 54-55 22.922133016330235 26.448370720109644 27.585775966462673 23.04372029709745 56-57 22.993861507723594 26.70969687611834 27.389232670391316 22.907208945766754 58-59 22.938603705058792 26.31964349389001 27.49981215728387 23.24194064376733 60-61 22.790250794699197 26.442833170102944 27.822799184786184 22.944116850411675 62-63 22.730057825109707 26.61775394828403 27.816137065779127 22.836051160827136 64-65 22.48047864437799 26.455419632330806 27.774934687826143 23.289167035465066 66-67 22.463514778702887 26.64759931607477 27.58900349037346 23.29988241484888 68-69 22.826100989277876 26.376620673878097 27.710702450808512 23.08657588603552 70-71 22.74239866478627 26.48942482425487 27.474955705461852 23.293220805497015 72-73 22.770723414627344 26.56076601277893 27.325327696957707 23.343182875636014 74-75 22.738241613989175 26.949179150298818 27.046030687813747 23.26654854789826 76-77 22.826437755266568 26.619465077993393 27.253314243580178 23.30078292315986 78-79 22.8169931974124 26.764100019158583 27.284841637020783 23.13406514640823 80-81 22.974604546776085 26.951290738873062 27.048298199436182 23.02580651491467 82-83 22.966337183812392 26.865134427661896 27.07523332415856 23.093295064367155 84-85 22.852495960431074 26.925837697375997 26.922558533082636 23.299107809110293 86-87 22.936073156143326 26.84452872052742 26.89058991936964 23.328808203959607 88-89 22.752779580269387 26.886103031572052 26.813439008866872 23.547678379291686 90-91 22.652042369861682 27.018910952026175 26.910977940461922 23.418068737650223 92-93 22.820943686679207 26.58555339641378 27.005699549024538 23.587803367882472 94-95 22.555357199108276 26.514831892480593 27.20701958048386 23.722791327927272 96-97 22.759310890078797 26.24609534157273 27.19013317538262 23.804460592965857 98-99 22.51115303162612 26.529213739027064 26.86461802383617 24.09501520551065 100-101 22.590805833510203 25.969742835793223 27.462254714384493 23.977196616312078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 995.0 1 663.0 2 465.5 3 614.5 4 553.0 5 516.5 6 904.5 7 2076.5 8 3292.0 9 3594.0 10 2940.5 11 2275.0 12 2196.5 13 2316.5 14 2517.0 15 2647.5 16 2739.5 17 2832.0 18 3035.0 19 3268.0 20 3520.5 21 4070.5 22 4862.0 23 5912.5 24 7213.0 25 8721.5 26 10859.5 27 13619.0 28 15930.5 29 18244.0 30 21300.0 31 24898.5 32 29188.0 33 31106.5 34 31506.5 35 32966.0 36 35599.0 37 39030.0 38 42216.5 39 44577.0 40 46568.0 41 50523.0 42 54165.5 43 56890.5 44 60166.5 45 64592.0 46 66730.5 47 68439.0 48 73430.5 49 74618.0 50 72707.5 51 71728.0 52 71113.0 53 72621.0 54 74210.5 55 70725.5 56 64530.0 57 59075.0 58 54801.0 59 48666.0 60 40092.5 61 33670.5 62 28655.5 63 22009.0 64 16130.0 65 12496.5 66 9699.0 67 8103.5 68 6291.5 69 4340.5 70 3174.5 71 2337.5 72 1856.0 73 1581.0 74 1286.0 75 1071.5 76 798.5 77 474.0 78 288.5 79 199.0 80 123.5 81 77.0 82 50.5 83 29.5 84 15.5 85 8.0 86 6.0 87 5.5 88 5.0 89 3.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27674081020661834 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023754575983400716 16-17 4.389432518671871E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.746057385891538E-5 26-27 0.001213548990456341 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003821388310373159 36-37 0.020888534750620847 38-39 0.006377587247717365 40-41 0.017428629118255958 42-43 0.005293139213692551 44-45 4.389432518671871E-4 46-47 1.0328076514522051E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.389432518671871E-4 60-61 0.0022979970244811562 62-63 0.0013684701381741716 64-65 0.0 66-67 0.0 68-69 3.098422954356615E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23457643783608206 78-79 0.0 80-81 0.001032807651452205 82-83 0.0 84-85 0.0 86-87 0.10772183804646498 88-89 0.36359993369374877 90-91 0.363109350059309 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.0328076514522051E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1936469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.26539486811509 #Duplication Level Percentage of deduplicated Percentage of total 1 74.89307301098032 39.892091068164405 2 13.908358670567008 14.816684331102481 3 4.693531200624407 7.500083781812321 4 2.1629409017523753 4.608396048329488 5 1.1559115600598109 3.0785042839602386 6 0.7185333834210245 2.296377863630617 7 0.4580171785251063 1.7077526109363825 8 0.3299009774776024 1.4057844666177308 9 0.24764663670278675 1.1871896302561122 >10 1.2956704748240118 12.468390652620922 >50 0.08811364783771984 3.2386208404691725 >100 0.04218099206227394 4.029819423956633 >500 0.004469568216090725 1.6619070103279783 >1k 0.0013603033701145679 1.0324618650628392 >5k 2.9149357931026456E-4 1.0759361227525874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8670 0.4477221169045309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7043 0.363703214458894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.26212658193856964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.164038257261025E-5 14-15 0.0 0.0 0.0 0.0 1.5492114771783075E-4 16-17 0.0 0.0 0.0 2.5820191286305125E-5 2.06561530290441E-4 18-19 0.0 0.0 0.0 5.164038257261025E-5 3.3566248672196663E-4 20-21 0.0 0.0 0.0 1.5492114771783075E-4 3.873028692945769E-4 22-23 0.0 0.0 0.0 2.840221041493564E-4 4.13123060580882E-4 24-25 0.0 0.0 0.0 4.6476344315349224E-4 4.13123060580882E-4 26-27 0.0 0.0 0.0 7.229653560165435E-4 4.13123060580882E-4 28-29 0.0 0.0 0.0 0.001445930712033087 4.13123060580882E-4 30-31 0.0 0.0 0.0 0.004905836344397974 4.13123060580882E-4 32-33 0.0 0.0 0.0 0.01179982741784144 4.6476344315349224E-4 34-35 0.0 0.0 0.0 0.021714780871782607 5.164038257261025E-4 36-37 0.0 0.0 0.0 0.038652826355598774 5.164038257261025E-4 38-39 0.0 0.0 0.0 0.06333692922530648 5.680442082987128E-4 40-41 0.0 0.0 0.0 0.09292686843941214 5.680442082987128E-4 42-43 0.0 0.0 0.0 0.12414347970455505 5.680442082987128E-4 44-45 0.0 0.0 0.0 0.16189259936513314 5.938643995850179E-4 46-47 0.0 0.0 0.0 0.2094792119057935 6.19684590871323E-4 48-49 0.0 0.0 0.0 0.2596736637663707 6.455047821576281E-4 50-51 0.0 0.0 0.0 0.3163489836398104 6.713249734439333E-4 52-53 0.0 0.0 0.0 0.37436695346013804 7.229653560165435E-4 54-55 0.0 0.0 0.0 0.44253225845598354 8.26246121161764E-4 56-57 0.0 0.0 0.0 0.5247179273202928 8.520663124480691E-4 58-59 0.0 0.0 0.0 0.6171542121252651 8.778865037343742E-4 60-61 0.0 0.0 0.0 0.7192472484713156 8.778865037343742E-4 62-63 0.0 0.0 0.0 0.8240255847111417 8.778865037343742E-4 64-65 0.0 0.0 0.0 0.9369114610148678 9.295268863069845E-4 66-67 2.5820191286305125E-5 0.0 0.0 1.0754626074571811 9.295268863069845E-4 68-69 5.164038257261025E-5 0.0 0.0 1.2325268310517752 9.295268863069845E-4 70-71 5.164038257261025E-5 0.0 0.0 1.4059610559218867 9.295268863069845E-4 72-73 5.164038257261025E-5 0.0 0.0 1.5921762754787192 9.553470775932897E-4 74-75 5.164038257261025E-5 0.0 0.0 1.785853530317294 9.811672688795948E-4 76-77 5.164038257261025E-5 0.0 0.0 2.001916891001095 0.001032807651452205 78-79 5.164038257261025E-5 0.0 0.0 2.25177888207867 0.001032807651452205 80-81 5.164038257261025E-5 0.0 0.0 2.5254729097135042 0.001032807651452205 82-83 5.164038257261025E-5 0.0 0.0 2.8178865760309098 0.0010844480340248153 84-85 5.164038257261025E-5 0.0 0.0 3.1191823881508043 0.0010844480340248153 86-87 5.164038257261025E-5 0.0 0.0 3.440798690813021 0.0011102682253111204 88-89 5.164038257261025E-5 0.0 0.0 3.7947160527744055 0.0011360884165974256 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5150 0.0 46.101795 1 GTATCAA 8615 0.0 37.99773 1 TATCAAC 11265 0.0 29.067543 2 ATCAACG 11310 0.0 28.784054 3 TCAACGC 11465 0.0 28.394909 4 CAACGCA 11755 0.0 27.573284 5 AACGCAG 12000 0.0 27.01033 6 GTAAGGT 955 0.0 24.349112 4 GTACATG 8480 0.0 24.238873 1 ACGCAGA 13585 0.0 23.824028 7 TACCGTG 180 1.9700565E-7 23.727962 7 CGCAGAG 13780 0.0 23.418016 8 TACATGG 8605 0.0 23.328129 2 ACATGGG 8575 0.0 22.57961 3 GAGTACT 8240 0.0 21.827986 12-13 TAAGGTG 1135 0.0 21.741922 5 GCAGAGT 14885 0.0 21.297531 9 CATGGGG 4530 0.0 21.26612 4 GGTAAGG 1050 0.0 20.790216 3 GTACTTT 8535 0.0 20.739916 14-15 >>END_MODULE