FastQCFastQC Report
Fri 27 May 2016
SRR939235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949500
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164080.8416517055655296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112030.5746601692741728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.48243139266478585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37820.19399846114388303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.1359835855347525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.11664529366504231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78900.037.7700731
GTATCAA129900.036.7497181
TCGCGAT300.003936860731.69166850-51
TATCACG1102.7730148E-630.1734162
ATCAACG165500.028.5351723
TCAACGC167050.028.2420184
TATCAAC168650.028.1146582
CAACGCA170100.027.7077475
GTACATG132200.027.3878691
TTCGCGA350.00836015627.16533948-49
AACGCAG174750.026.9538386
TACATGG134300.026.5144772
ACATGGG136000.025.067393
ACGCAGA196600.023.9341057
CATGGGG50850.023.777624
CGCAGAG197550.023.7716458
GAGTACT101800.022.76240212-13
GCAGAGT205450.022.5802129
AGTACTT106450.022.1909812-13
CATGGGA78350.021.3626374
Produced by FastQC (version 0.11.5)