FastQCFastQC Report
Fri 27 May 2016
SRR939235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949500
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169290.8683765067966145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129310.6632982816106694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106150.5444985893818928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38400.19697358296999232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.15091048986919722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.14372916132341626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.037.71251
GTATCAA149550.036.5171851
ATCAACG193750.027.9737973
TATCAAC195350.027.8904482
TCAACGC195100.027.7802354
CAACGCA198600.027.3384485
AACGCAG203250.026.7596936
ACGCAGA224150.024.1798957
CGCAGAG226050.024.0396448
AGTACTT127850.023.92669312-13
GAGTACT125250.023.0591512-13
GTACATG124900.022.9285141
GCAGAGT234500.022.8507489
TACATGG126400.022.4908982
GTACTTT131800.021.71512614-15
AGAGTAC209800.021.15246810-11
TACTTTT143200.021.14648214-15
ACATGGG127450.021.1139893
ACTTTTT149050.019.36019916-17
AGTACAT99400.019.2418022
Produced by FastQC (version 0.11.5)