##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939235_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1949500 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977637342908437 31.0 31.0 34.0 30.0 34.0 2 32.20777840471916 33.0 31.0 34.0 30.0 34.0 3 32.27385432162093 34.0 31.0 34.0 30.0 34.0 4 35.914385739933316 37.0 35.0 37.0 35.0 37.0 5 35.853169530648884 37.0 35.0 37.0 35.0 37.0 6 35.897701461913314 37.0 35.0 37.0 35.0 37.0 7 35.8309776865863 37.0 35.0 37.0 35.0 37.0 8 35.79652577583996 37.0 35.0 37.0 33.0 37.0 9 37.42443600923313 39.0 37.0 39.0 34.0 39.0 10-11 37.322502436522186 39.0 37.0 39.0 34.0 39.0 12-13 37.220465503975376 39.0 37.0 39.0 33.0 39.0 14-15 38.34037496794049 40.0 38.0 41.0 33.0 41.0 16-17 38.33496409335727 40.0 38.0 41.0 33.5 41.0 18-19 38.50035598871506 40.0 38.0 41.0 34.0 41.0 20-21 38.484789433188 40.0 38.0 41.0 34.0 41.0 22-23 38.3802433957425 40.0 38.0 41.0 34.0 41.0 24-25 38.27680892536548 40.0 38.0 41.0 33.5 41.0 26-27 38.05844139522954 40.0 37.0 41.0 33.0 41.0 28-29 37.82785201333675 40.0 37.0 41.0 33.0 41.0 30-31 37.526843806104125 39.5 36.5 41.0 32.0 41.0 32-33 37.36698332905873 39.0 36.0 41.0 32.0 41.0 34-35 37.199616311874834 39.0 36.0 41.0 31.5 41.0 36-37 36.970020518081554 39.0 35.0 41.0 31.0 41.0 38-39 36.78115311618363 39.0 35.0 41.0 31.0 41.0 40-41 36.50318389330597 39.0 35.0 40.5 30.0 41.0 42-43 36.21711618363683 39.0 35.0 40.0 30.0 41.0 44-45 35.832030007694286 38.5 35.0 40.0 29.0 41.0 46-47 35.350378558604774 38.0 35.0 40.0 27.0 41.0 48-49 34.89241626057964 38.0 34.0 40.0 25.5 41.0 50-51 34.55397024878174 38.0 34.0 40.0 24.0 41.0 52-53 34.219689664016414 38.0 33.0 40.0 23.5 41.0 54-55 33.9047986663247 37.5 33.0 40.0 23.0 41.0 56-57 33.51041985124391 37.0 33.0 40.0 22.0 41.0 58-59 33.12104642215953 36.5 32.0 39.5 21.0 41.0 60-61 32.720064119004874 36.0 32.0 39.0 19.5 40.0 62-63 32.28110695050013 35.5 31.0 39.0 18.0 40.0 64-65 31.883465760451397 35.0 31.0 38.5 15.5 40.0 66-67 31.388780969479356 35.0 30.5 38.0 12.0 40.0 68-69 30.83365016670941 35.0 29.5 37.5 8.0 40.0 70-71 30.27026365734804 34.0 29.0 37.0 7.0 39.0 72-73 29.710774044626827 34.0 28.5 36.0 2.0 39.0 74-75 29.110119517825083 34.0 28.0 36.0 2.0 38.5 76-77 27.332756347781483 31.5 25.5 34.0 2.0 36.0 78-79 28.302457296742755 33.0 27.0 35.0 2.0 37.0 80-81 28.265912285201335 33.0 27.0 35.0 2.0 37.0 82-83 27.818852526288794 33.0 26.0 35.0 2.0 36.0 84-85 27.241279815337265 33.0 26.0 35.0 2.0 36.0 86-87 26.462284688381636 32.0 24.5 34.5 2.0 35.0 88-89 25.69348089253655 31.5 22.0 34.0 2.0 35.0 90-91 25.00557065914337 31.0 19.5 34.0 2.0 35.0 92-93 24.428778661195178 31.0 17.5 34.0 2.0 35.0 94-95 23.651855604001028 31.0 5.0 34.0 2.0 35.0 96-97 22.593259810207748 30.0 2.0 34.0 2.0 35.0 98-99 21.218456783790714 29.5 2.0 34.0 2.0 35.0 100-101 17.10132392921262 22.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28550720872715374 1101 2 0.34870271154940014 1101 3 0.3776538821233544 1101 4 0.17270747159378175 1101 5 0.19616371102974028 1101 6 0.195854048924339 1101 7 0.26031864420233575 1101 8 0.29283433965800754 1101 9 0.4210486833833542 1101 10-11 0.37032750429079186 1101 12-13 0.43463813743545643 1101 14-15 0.4495628821037414 1101 16-17 0.409655250169223 1101 18-19 0.5477496094009027 1101 20-21 0.43822698577482555 1101 22-23 0.42871156401120203 1101 24-25 0.4890750310118861 1101 26-27 0.5619493610319566 1101 28-29 0.5145387912139583 1101 30-31 0.4661730772219599 1101 32-33 0.7111851467004087 1101 34-35 0.743606229907428 1101 36-37 0.6656904527851495 1101 38-39 0.8686065417497346 1101 40-41 0.8238866159051597 1101 42-43 0.7935805191045233 1101 44-45 0.9309736180112509 1101 46-47 0.9902028194185135 1101 48-49 0.9138157796702728 1101 50-51 0.8120334202914066 1101 52-53 0.8491730508757414 1101 54-55 0.8691053664473927 1101 56-57 0.760858901306996 1101 58-59 0.8722542724676359 1101 60-61 0.9294655388017716 1101 62-63 0.839086267475956 1101 64-65 0.7011154756266116 1101 66-67 0.7787519492012436 1101 68-69 0.8949028624784212 1101 70-71 0.6815916247639429 1101 72-73 0.7737090525488384 1101 74-75 0.7771080643986217 1101 76-77 0.7557946915444802 1101 78-79 0.7953828374658372 1101 80-81 0.9390344033966933 1101 82-83 0.963564673016176 1101 84-85 0.8542666914327306 1101 86-87 0.7012351711088591 1101 88-89 0.9298533370242872 1101 90-91 1.1442238411628516 1101 92-93 0.9985286390133439 1101 94-95 1.0373612615729648 1101 96-97 0.6705700471412364 1101 98-99 0.5384197226728311 1101 100-101 -0.07211116985131838 1102 1 0.2224064198389044 1102 2 0.22678730039462636 1102 3 0.27761857979248816 1102 4 0.23622643023664835 1102 5 0.29332523342588956 1102 6 0.312889052227149 1102 7 0.25132550013214683 1102 8 0.2535651184485559 1102 9 0.3549787425110935 1102 10-11 0.3639832088452053 1102 12-13 0.41842136762513604 1102 14-15 0.4493907722363062 1102 16-17 0.43991239962484485 1102 18-19 0.3971911840433222 1102 20-21 0.46006454399213226 1102 22-23 0.4909378645704905 1102 24-25 0.43650287618754646 1102 26-27 0.4160136922670006 1102 28-29 0.448707573917396 1102 30-31 0.46802021372754155 1102 32-33 0.5642708139834198 1102 34-35 0.6928234014541559 1102 36-37 0.6762176195867582 1102 38-39 0.6476211401292318 1102 40-41 0.7493844653388066 1102 42-43 0.7030651080084382 1102 44-45 0.6630725044472072 1102 46-47 0.7061208839704278 1102 48-49 0.6025670426162932 1102 50-51 0.6958281538174944 1102 52-53 0.7020560892355476 1102 54-55 0.5944834579323768 1102 56-57 0.47298306573779314 1102 58-59 0.5890833601796928 1102 60-61 0.7022702429620722 1102 62-63 0.7447006586193297 1102 64-65 0.6274021252710789 1102 66-67 0.6753061423874556 1102 68-69 0.6414887632028687 1102 70-71 0.5623890348699199 1102 72-73 0.6706020365994014 1102 74-75 0.588344036270616 1102 76-77 0.5461146926478619 1102 78-79 0.5889046983535273 1102 80-81 0.8560451675263572 1102 82-83 0.9181520245695829 1102 84-85 0.8029442039066623 1102 86-87 0.492031885732505 1102 88-89 0.6310707538972729 1102 90-91 0.8485867749479432 1102 92-93 1.0297682673029804 1102 94-95 0.7900398222590788 1102 96-97 0.19829660931339532 1102 98-99 0.2987642886815891 1102 100-101 -0.29306253098653556 1103 1 0.142080766332505 1103 2 0.19550350735333666 1103 3 0.2383748804955701 1103 4 0.19459989336987604 1103 5 0.15570619610841874 1103 6 0.18347423340616587 1103 7 0.26173141323832994 1103 8 0.22070267027837076 1103 9 0.3700952379140148 1103 10-11 0.2702937911419454 1103 12-13 0.2694369075566314 1103 14-15 0.3214576912916556 1103 16-17 0.20150014074617673 1103 18-19 0.19679486170692684 1103 20-21 0.22570117640604792 1103 22-23 0.26344985303272495 1103 24-25 0.39488931930995363 1103 26-27 0.30917410329998773 1103 28-29 0.32928532765816243 1103 30-31 0.39686564368967936 1103 32-33 0.39335161441609046 1103 34-35 0.28603126155872616 1103 36-37 0.20967902214669465 1103 38-39 0.34787194236466945 1103 40-41 0.5212072163754016 1103 42-43 0.3770813329949263 1103 44-45 0.44891378802390847 1103 46-47 0.6317530454991811 1103 48-49 0.5785299775360713 1103 50-51 0.5431771352489037 1103 52-53 0.27382937209115 1103 54-55 0.32192055906812556 1103 56-57 0.49472224006626675 1103 58-59 0.487484802400715 1103 60-61 0.5306485375357255 1103 62-63 0.6737878048244852 1103 64-65 0.5321846743967278 1103 66-67 0.39433581608494706 1103 68-69 0.2824579357285586 1103 70-71 0.23039350575508166 1103 72-73 0.20076363746473547 1103 74-75 0.2651267296183697 1103 76-77 0.3900750315698005 1103 78-79 0.4553029676883007 1103 80-81 0.5815848826877037 1103 82-83 0.7158335100572337 1103 84-85 0.7271867854822887 1103 86-87 0.6665560609623569 1103 88-89 1.0079539700501492 1103 90-91 1.0548679359358637 1103 92-93 0.9283407569370183 1103 94-95 0.7771377130108377 1103 96-97 0.7504389565281073 1103 98-99 0.1836701477044862 1103 100-101 0.12991104031326728 1104 1 0.26313352489202657 1104 2 0.19355126325186944 1104 3 0.16382118171962645 1104 4 0.1054798649859805 1104 5 0.20356654735908108 1104 6 0.16891755432154554 1104 7 0.16783863339089322 1104 8 0.26481265786041774 1104 9 0.3087655944866583 1104 10-11 0.2522414938206694 1104 12-13 0.22686334005619102 1104 14-15 0.23965900404666485 1104 16-17 0.20192391225585027 1104 18-19 0.23439401019016515 1104 20-21 0.4021790192302461 1104 22-23 0.4461881890270476 1104 24-25 0.3151870663385097 1104 26-27 0.4374095476853057 1104 28-29 0.41563962959180856 1104 30-31 0.4581859204326264 1104 32-33 0.43983463730059924 1104 34-35 0.5640507754845672 1104 36-37 0.6498779932306107 1104 38-39 0.6237358416018637 1104 40-41 0.7584119706773436 1104 42-43 0.8033314660444191 1104 44-45 0.6062823173834957 1104 46-47 0.6593858998543354 1104 48-49 0.8414403199167637 1104 50-51 0.8404095638456752 1104 52-53 0.7706123417558643 1104 54-55 0.6662552051564674 1104 56-57 0.6308990717143033 1104 58-59 0.8243574651646028 1104 60-61 0.8331352503262082 1104 62-63 0.9039537796337811 1104 64-65 0.8263012612336986 1104 66-67 0.7092321084390392 1104 68-69 0.866230154994124 1104 70-71 0.7191469561719721 1104 72-73 0.7365247870123675 1104 74-75 0.7613293906082568 1104 76-77 0.7229634473938198 1104 78-79 0.8873634253785632 1104 80-81 1.023822810663578 1104 82-83 0.9447738684038711 1104 84-85 0.8428927283597041 1104 86-87 0.8266203504780556 1104 88-89 1.0000002357943423 1104 90-91 1.156039906842377 1104 92-93 1.0108077429224736 1104 94-95 1.1726544772471321 1104 96-97 0.9183606169857974 1104 98-99 0.9872023811619961 1104 100-101 1.035001975207713 1105 1 0.14887351389177894 1105 2 0.0911983469628268 1105 3 0.13884601020268406 1105 4 0.09772597426248097 1105 5 0.13078402037619696 1105 6 0.07403071876181144 1105 7 0.06459389580457753 1105 8 0.08519778882788387 1105 9 0.1640517232696581 1105 10-11 0.19137775209033947 1105 12-13 0.18002240267517777 1105 14-15 0.2009176633976537 1105 16-17 0.12206262331242357 1105 18-19 0.08075218388265881 1105 20-21 0.10681637863756777 1105 22-23 0.21984594787233647 1105 24-25 0.18122042112305792 1105 26-27 0.2663933501619198 1105 28-29 0.1787374894433711 1105 30-31 0.2499212922252454 1105 32-33 0.11682692822362384 1105 34-35 0.2128422796898235 1105 36-37 0.05512267626106393 1105 38-39 0.15957250024472813 1105 40-41 0.2747618886208727 1105 42-43 0.2767528811120812 1105 44-45 0.2195377768663107 1105 46-47 0.2315332407572015 1105 48-49 0.2524729343003642 1105 50-51 0.46544310456550164 1105 52-53 0.3876293720911477 1105 54-55 0.3647000569760692 1105 56-57 0.4059960197036432 1105 58-59 0.3942444955666815 1105 60-61 0.4768153436723992 1105 62-63 0.5910640949221104 1105 64-65 0.5650517594734374 1105 66-67 0.487371241468491 1105 68-69 0.558621115644879 1105 70-71 0.36939796879552844 1105 72-73 0.6046891604772888 1105 74-75 0.6683037170660704 1105 76-77 0.7042619213884898 1105 78-79 0.683223888190394 1105 80-81 0.7820565702748752 1105 82-83 0.7765187262357536 1105 84-85 0.8844467575882895 1105 86-87 0.9273627555230277 1105 88-89 1.0376391862286702 1105 90-91 1.1062903906081125 1105 92-93 0.7976990554028482 1105 94-95 0.8815456628016314 1105 96-97 0.7749443958586504 1105 98-99 0.819192044496674 1105 100-101 0.6360743596995988 1106 1 0.2126900490329291 1106 2 0.16324696726895382 1106 3 0.20639513366013063 1106 4 0.10679567396059042 1106 5 0.09709353788056774 1106 6 0.1562337270770513 1106 7 0.17483858623410953 1106 8 0.07288832428680792 1106 9 0.2627737189266668 1106 10-11 0.25130307384235806 1106 12-13 0.20873142232456132 1106 14-15 0.20323321871781275 1106 16-17 0.12423769348879432 1106 18-19 0.20095688702338066 1106 20-21 0.21053493168030712 1106 22-23 0.34630217370782646 1106 24-25 0.38678130243231124 1106 26-27 0.37720448304683174 1106 28-29 0.3872566356750369 1106 30-31 0.48345804875297205 1106 32-33 0.3675490827705161 1106 34-35 0.4462675484785521 1106 36-37 0.43187986602855233 1106 38-39 0.5667107609300714 1106 40-41 0.5448494104682311 1106 42-43 0.4753598140075823 1106 44-45 0.5225272901336169 1106 46-47 0.47785599908568344 1106 48-49 0.4188911589706734 1106 50-51 0.2778573040252681 1106 52-53 0.3580724100658301 1106 54-55 0.4105889134985006 1106 56-57 0.5860673877455866 1106 58-59 0.484240920544174 1106 60-61 0.3596679468184192 1106 62-63 0.49735573731393146 1106 64-65 0.6145272904304839 1106 66-67 0.7114261114436005 1106 68-69 0.5615465433234981 1106 70-71 0.3365673454175315 1106 72-73 0.3951332577178981 1106 74-75 0.4667064764538118 1106 76-77 0.5963703902195903 1106 78-79 0.6074751195870327 1106 80-81 0.4656996506201949 1106 82-83 0.748225071238668 1106 84-85 0.7787243382249045 1106 86-87 0.794414288810458 1106 88-89 1.0271303413581698 1106 90-91 1.101983547750212 1106 92-93 1.1872707147429225 1106 94-95 1.3365807509855223 1106 96-97 1.2403731337432973 1106 98-99 1.3155317510800124 1106 100-101 1.1183397322963877 1107 1 0.08606754096138758 1107 2 0.09979311599031604 1107 3 0.12264532583969867 1107 4 0.06933295679767326 1107 5 0.10268109489383903 1107 6 0.11217463826446306 1107 7 0.08576002322483589 1107 8 0.12001306164139436 1107 9 0.11413896261036882 1107 10-11 0.08602671961697439 1107 12-13 0.1369003353568985 1107 14-15 0.21357901382877031 1107 16-17 0.19864373048976347 1107 18-19 0.1585900034073333 1107 20-21 0.18234193214154004 1107 22-23 0.2426880446656483 1107 24-25 0.23360213982277145 1107 26-27 0.18643807091132913 1107 28-29 0.1695223047161889 1107 30-31 0.2197781942969641 1107 32-33 0.19357954963876267 1107 34-35 0.292833555755756 1107 36-37 0.28287240946114167 1107 38-39 0.37699326490178464 1107 40-41 0.4420676752148154 1107 42-43 0.3894578512135425 1107 44-45 0.4226286328282285 1107 46-47 0.33926006304303513 1107 48-49 0.28941837159815265 1107 50-51 0.4869363795134092 1107 52-53 0.5245220846417524 1107 54-55 0.6459950529952536 1107 56-57 0.6472403237369804 1107 58-59 0.6395416175154267 1107 60-61 0.6414944214763381 1107 62-63 0.5877767386976274 1107 64-65 0.635759033371091 1107 66-67 0.8064946554911501 1107 68-69 0.9381016604249162 1107 70-71 0.780828863379913 1107 72-73 0.8457174836185821 1107 74-75 0.5964583085657402 1107 76-77 0.7178496604496907 1107 78-79 1.014640214651866 1107 80-81 1.020979079718746 1107 82-83 1.1694737259816606 1107 84-85 0.9803570958736536 1107 86-87 0.9536690164279449 1107 88-89 1.410098234557573 1107 90-91 1.4574255607671738 1107 92-93 1.3486345491097573 1107 94-95 1.5928988466140765 1107 96-97 1.2417098067305368 1107 98-99 1.0763882313752013 1107 100-101 0.7442522009070558 1108 1 0.04849099169409499 1108 2 0.07207962575226645 1108 3 0.003858634279630735 1108 4 0.13192972855318885 1108 5 0.07251588329307879 1108 6 0.021811266705860533 1108 7 0.07537608192316014 1108 8 0.07229769214180948 1108 9 0.14071723589584906 1108 10-11 0.06154397614025697 1108 12-13 0.08306664183177759 1108 14-15 0.06327511476961689 1108 16-17 0.15182815958236517 1108 18-19 0.20851554788250581 1108 20-21 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-0.5123734354855927 2316 64-65 -0.4464323798668346 2316 66-67 -0.3217355017445094 2316 68-69 -0.4281002038063271 2316 70-71 -0.42639409114414306 2316 72-73 -0.06020380439573003 2316 74-75 -0.2659988517769811 2316 76-77 -0.5187311699805903 2316 78-79 -0.5505761020791411 2316 80-81 -0.5142414739014392 2316 82-83 -0.4300145519582692 2316 84-85 -0.19890003622314012 2316 86-87 -0.3456857995027569 2316 88-89 -0.7573824640583773 2316 90-91 -0.8190173353819645 2316 92-93 -0.5329088554660828 2316 94-95 0.12881213161548644 2316 96-97 0.4090808169932245 2316 98-99 0.5116577446037063 2316 100-101 0.7553311953520279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 43.0 5 103.0 6 238.0 7 444.0 8 1110.0 9 1872.0 10 3105.0 11 4694.0 12 6601.0 13 8948.0 14 11482.0 15 15172.0 16 20251.0 17 27632.0 18 25803.0 19 17825.0 20 16783.0 21 18086.0 22 19325.0 23 21213.0 24 24457.0 25 28451.0 26 33270.0 27 39411.0 28 47533.0 29 56849.0 30 67719.0 31 80618.0 32 99629.0 33 125135.0 34 165472.0 35 226004.0 36 290010.0 37 314494.0 38 126468.0 39 3239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67255921694158 20.53501997556028 15.790567256538859 21.00185355095928 2 16.227955886124647 22.11408053347012 38.88971531161836 22.76824826878687 3 19.328648371377277 26.53300846370864 30.658579122852014 23.47976404206207 4 13.303565016670941 17.938189279302385 36.54460117978969 32.21364452423698 5 13.327571172095409 38.60051295203898 33.11161836368299 14.96029751218261 6 30.814311361887665 37.00938702231341 17.04724288279046 15.129058733008463 7 27.72033854834573 34.400769428058474 20.26386252885355 17.61502949474224 8 26.97773788150808 34.63123877917415 19.42262118491921 18.968402154398564 9 27.246935111567065 16.634932033854835 20.193177737881506 35.92495511669659 10-11 25.137522441651704 27.3622210823288 26.37468581687612 21.12557065914337 12-13 26.382431392664785 24.94526801744037 27.55642472428828 21.115875865606565 14-15 23.04058596153486 25.663773330265848 26.925802502668024 24.369838205531273 16-17 21.316224878935945 29.168890545167198 27.366106270657703 22.14877830523915 18-19 21.246068542207887 26.52329482515897 31.308907747860417 20.921728884772733 20-21 22.418876635034625 25.67573736855604 31.13441908181585 20.770966914593487 22-23 21.866760707873816 25.851013080276996 31.151654270325725 21.130571941523467 24-25 21.662734034367787 26.04557578866376 31.359194665298794 20.932495511669657 26-27 21.412398775275083 26.320531582729718 31.231229151694873 21.035840490300327 28-29 21.170223134136958 26.442600666837652 31.48294434470377 20.90423185432162 30-31 21.286098999743526 26.179456270838674 31.28456014362657 21.24988458579123 32-33 21.114773018722747 26.383482944344706 31.044293408566297 21.45745062836625 34-35 21.68487499705049 26.340848864873294 30.8797911641807 21.094484973895522 36-37 21.994178147456665 25.921889503813905 30.796517558535935 21.287414790193495 38-39 21.554706423684316 26.2284284426005 30.931979510034154 21.28488562368103 40-41 21.509528909900094 26.20119357598813 31.00070994892882 21.288567565182955 42-43 21.758607025656612 26.30949770389549 30.412785858920056 21.519109411527843 44-45 21.54670243707264 26.568554530205645 30.19946446983026 21.68527856289145 46-47 21.936185623170683 26.840114737229793 29.564457106393544 21.659242533205987 48-49 22.00707824152454 26.90244732547472 29.26880313393901 21.82167129906173 50-51 21.989695303845934 27.23988900740934 29.02211567635693 21.748300012387794 52-53 22.323117193819737 27.242822122834763 28.737156302369275 21.69690438097622 54-55 21.686765837394205 27.228468838163632 28.892203129007438 22.192562195434725 56-57 21.941215591135993 27.50205888289669 28.658624007074707 21.898101518892606 58-59 22.02253352354073 26.993933163747126 28.878566324570354 22.10496698814179 60-61 21.89536038172201 27.216211079715297 29.211562151269455 21.676866387293238 62-63 21.904233068176136 27.203589689005614 29.12521207660021 21.766965166218043 64-65 21.62031290074378 27.06758143113619 29.108361118235443 22.203744549884586 66-67 21.680177461752187 27.32011261403941 28.857334775419524 22.142375148788876 68-69 21.755006556915387 27.218623948014546 28.986541716370382 22.03982777869968 70-71 21.71174660169274 27.363118748397024 28.81472172351885 22.110412926391383 72-73 21.762118491921008 27.36329828161067 28.581815850218007 22.292767376250318 74-75 21.660456953182088 27.705008388050366 28.285542007063864 22.348992651703682 76-77 21.639748525205324 27.423982452090062 28.584407925147836 22.351861097556775 78-79 21.685476656530714 27.394824098125873 28.567178388670712 22.3525208566727 80-81 21.527778312103504 27.863385308265336 28.33212148258718 22.27671489704398 82-83 21.68976660682226 27.50882277507053 28.35432162092844 22.447088997178763 84-85 21.462015901513208 27.407078738137987 28.31241343934342 22.818491921005386 86-87 21.7210492806605 27.152832688101373 28.34944701738532 22.776671013852805 88-89 21.529877078017705 27.136001713011837 28.3346982180662 22.999422990904257 90-91 21.32926352956483 27.229064796223113 28.458465400111233 22.98320627410082 92-93 21.379302385226982 27.057912285201336 28.43080276994101 23.131982559630675 94-95 21.17907155680944 26.826032315978456 28.485278276481147 23.509617850730958 96-97 21.28463708643242 26.697332649397282 28.508232880225698 23.5097973839446 98-99 21.038426835229806 26.81019770740863 28.303711162417795 23.847664294943772 100-101 21.12702209781371 26.06600233958445 29.124158787047804 23.682816775554034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3671.0 1 2426.5 2 1565.5 3 1930.5 4 1595.5 5 1449.5 6 2610.5 7 5879.5 8 8941.5 9 9071.5 10 6704.0 11 4553.0 12 4165.0 13 4307.5 14 4459.0 15 4561.5 16 4662.5 17 4816.0 18 4958.0 19 5162.5 20 5483.0 21 6013.0 22 6837.0 23 8122.0 24 9459.5 25 10680.5 26 12726.0 27 15371.0 28 17493.5 29 19895.5 30 22835.0 31 25683.5 32 29458.5 33 32692.0 34 35520.5 35 38328.5 36 41648.0 37 45883.0 38 49188.5 39 50303.0 40 50874.5 41 55005.5 42 59617.0 43 62658.5 44 65282.5 45 68695.5 46 69341.0 47 69117.0 48 76067.5 49 77379.5 50 70786.5 51 66643.5 52 63690.5 53 62877.5 54 62422.5 55 59038.5 56 53262.0 57 47066.0 58 42616.5 59 37940.5 60 32174.5 61 27214.5 62 22960.0 63 17783.0 64 13366.0 65 10590.0 66 8012.5 67 6526.0 68 5052.5 69 3430.5 70 2606.5 71 2081.5 72 1752.0 73 1581.5 74 1325.0 75 1116.5 76 826.0 77 505.0 78 336.5 79 235.5 80 146.0 81 87.5 82 61.5 83 31.5 84 14.0 85 8.5 86 5.5 87 4.0 88 3.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2628366247755835 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010515516799179277 16-17 7.950756604257502E-4 18-19 2.564760194921775E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.12952038984355E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001333675301359323 36-37 0.006181072069761478 38-39 0.0029751218261092585 40-41 0.003282893049499872 42-43 1.2823800974608876E-4 44-45 0.001538856116953065 46-47 1.02590407796871E-4 48-49 2.3082841754295971E-4 50-51 2.564760194921775E-5 52-53 1.5388561169530648E-4 54-55 0.0 56-57 0.0010515516799179277 58-59 0.0025134649910233393 60-61 9.489612721210566E-4 62-63 0.0014106181072069761 64-65 0.0 66-67 4.873044370351373E-4 68-69 0.001615798922800718 70-71 0.0 72-73 0.0 74-75 2.564760194921775E-5 76-77 0.23867658373942038 78-79 2.05180815593742E-4 80-81 1.2823800974608876E-4 82-83 0.0 84-85 0.0 86-87 0.09643498332905873 88-89 0.3448576558091819 90-91 0.2531161836368299 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.5388561169530648E-4 100-101 4.360092331367017E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1949500.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.06317846139209 #Duplication Level Percentage of deduplicated Percentage of total 1 70.21556828253294 30.939211160013112 2 14.834794086353778 13.073363585300212 3 5.818752702313621 7.691782162342579 4 2.8889405907377217 5.091836192561429 5 1.6184960592924544 3.565804034983163 6 1.0112635176864937 2.6735690910789067 7 0.6773835849650254 2.0893371653792028 8 0.4779862484039562 1.684927469241185 9 0.3537602909644849 1.4029022549989885 >10 1.8501581691402036 14.923414787008578 >50 0.1582098363038058 4.85737642121067 >100 0.08827383835718082 7.307882928823542 >500 0.004547253181509639 1.3612586892576946 >1k 0.001515751060503213 1.25789016258248 >5k 0.0 0.0 >10k+ 3.497887062699722E-4 2.079443895218342 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16929 0.8683765067966145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12931 0.6632982816106694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10615 0.5444985893818928 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3840 0.19697358296999232 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2942 0.15091048986919722 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2802 0.14372916132341626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.02590407796871E-4 0.0 0.0 0.0 0.0 3 1.02590407796871E-4 0.0 0.0 0.0 0.0 4 1.02590407796871E-4 0.0 0.0 0.0 0.0 5 1.02590407796871E-4 0.0 0.0 0.0 0.0 6 1.02590407796871E-4 0.0 0.0 0.0 0.0 7 1.02590407796871E-4 0.0 0.0 0.0 0.0 8 1.02590407796871E-4 0.0 0.0 0.0 0.0 9 1.02590407796871E-4 0.0 0.0 0.0 0.0 10-11 1.02590407796871E-4 0.0 0.0 0.0 0.0 12-13 1.02590407796871E-4 0.0 0.0 0.0 0.0 14-15 1.02590407796871E-4 0.0 0.0 0.0 0.0 16-17 1.02590407796871E-4 0.0 0.0 0.0 0.0 18-19 1.2823800974608873E-4 0.0 0.0 0.0 0.0 20-21 1.5388561169530648E-4 0.0 0.0 2.564760194921775E-5 0.0 22-23 1.5388561169530648E-4 0.0 0.0 1.2823800974608873E-4 0.0 24-25 2.05180815593742E-4 0.0 0.0 3.334188253398307E-4 0.0 26-27 2.05180815593742E-4 0.0 0.0 8.720184662734035E-4 0.0 28-29 3.590664272890485E-4 0.0 0.0 0.002359579379328033 0.0 30-31 3.590664272890485E-4 0.0 0.0 0.007130033341882534 0.0 32-33 3.590664272890485E-4 0.0 0.0 0.01585021800461657 0.0 34-35 3.8471402923826624E-4 0.0 0.0 0.027622467299307514 0.0 36-37 4.10361631187484E-4 0.0 0.0 0.04808925365478328 0.0 38-39 4.10361631187484E-4 0.0 0.0 0.0816363170043601 0.0 40-41 4.10361631187484E-4 0.0 0.0 0.12003077712233906 0.0 42-43 4.616568350859195E-4 0.0 0.0 0.16109258784303668 0.0 44-45 4.616568350859195E-4 0.0 0.0 0.21015645037189024 0.0 46-47 5.642472428827904E-4 0.0 0.0 0.27191587586560656 0.0 48-49 6.155424467812259E-4 0.0 0.0 0.3401384970505258 0.0 50-51 6.155424467812259E-4 0.0 0.0 0.4185945114131829 0.0 52-53 6.155424467812259E-4 0.0 0.0 0.4973326493972814 0.0 54-55 6.668376506796615E-4 0.0 0.0 0.5863298281610669 0.0 56-57 6.668376506796615E-4 0.0 0.0 0.6935111567068479 0.0 58-59 6.668376506796615E-4 0.0 0.0 0.8149781995383432 0.0 60-61 6.668376506796615E-4 0.0 0.0 0.9579892280071813 0.0 62-63 6.668376506796615E-4 0.0 0.0 1.106104129263914 0.0 64-65 6.668376506796615E-4 0.0 0.0 1.2583226468325213 0.0 66-67 6.668376506796615E-4 0.0 0.0 1.4315465503975378 0.0 68-69 6.668376506796615E-4 0.0 0.0 1.6304437035137216 0.0 70-71 7.18132854578097E-4 0.0 0.0 1.8654270325724545 0.0 72-73 7.18132854578097E-4 0.0 0.0 2.108489356245191 0.0 74-75 7.18132854578097E-4 0.0 0.0 2.356552962298025 0.0 76-77 7.18132854578097E-4 0.0 0.0 2.6277763529110025 0.0 78-79 7.18132854578097E-4 0.0 0.0 2.944755065401385 0.0 80-81 8.20723262374968E-4 0.0 0.0 3.297255706591434 0.0 82-83 8.720184662734035E-4 0.0 0.0 3.6680943831751733 0.0 84-85 8.976660682226212E-4 0.0 0.0 4.0416260579635805 0.0 86-87 9.23313670171839E-4 0.0 0.0 4.433239292126187 0.0 88-89 9.23313670171839E-4 0.0 0.0 4.855706591433701 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9120 0.0 37.7125 1 GTATCAA 14955 0.0 36.517185 1 ATCAACG 19375 0.0 27.973797 3 TATCAAC 19535 0.0 27.890448 2 TCAACGC 19510 0.0 27.780235 4 CAACGCA 19860 0.0 27.338448 5 AACGCAG 20325 0.0 26.759693 6 ACGCAGA 22415 0.0 24.179895 7 CGCAGAG 22605 0.0 24.039644 8 AGTACTT 12785 0.0 23.926693 12-13 GAGTACT 12525 0.0 23.05915 12-13 GTACATG 12490 0.0 22.928514 1 GCAGAGT 23450 0.0 22.850748 9 TACATGG 12640 0.0 22.490898 2 GTACTTT 13180 0.0 21.715126 14-15 AGAGTAC 20980 0.0 21.152468 10-11 TACTTTT 14320 0.0 21.146482 14-15 ACATGGG 12745 0.0 21.113989 3 ACTTTTT 14905 0.0 19.360199 16-17 AGTACAT 9940 0.0 19.241802 2 >>END_MODULE