FastQCFastQC Report
Fri 27 May 2016
SRR939234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987967
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165540.8327099997132749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112000.5633896337313447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94580.475762424627773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39500.19869545118203674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28240.14205467193368904No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.12087725802289474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134900.037.2211531
GGTATCA81450.036.6264951
ATCAACG173600.028.6044923
TATCAAC174400.028.473282
TCAACGC174950.028.3566554
CAACGCA178650.027.7693655
AACGCAG181750.027.277866
GTACATG134700.026.9081461
TACATGG135900.026.3141382
ACATGGG135650.025.1389083
ACGCAGA204850.024.109317
CGCAGAG205850.023.9691648
CATGGGG52000.023.8965514
ATGGGGA28500.022.6324395
GAGTACT106450.022.59718512-13
GCAGAGT215900.022.5021659
AGTACTT110650.022.3605812-13
TACCGTA1302.9859578E-421.875657
CATGGGA77950.021.6606354
CTATACG1150.00375696720.6209874
Produced by FastQC (version 0.11.5)