FastQCFastQC Report
Fri 27 May 2016
SRR939234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987967
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173190.8711915238029605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128270.6452320385599962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.5531781966199639No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38650.19441972628318277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.15679334717326798No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.14285951426759097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96500.036.2327731
GTATCAA156750.035.5074841
ATCAACG198600.027.8134253
TCAACGC199650.027.690924
TATCAAC202200.027.412112
CAACGCA202750.027.2441235
AACGCAG208000.026.6477326
ACGCAGA230750.023.9587967
CGCAGAG231950.023.7734668
AGTACTT129600.023.71091512-13
GTACATG126250.023.6630021
TATCACG1452.3526763E-522.9092452
GAGTACT126100.022.76951612-13
GCAGAGT239850.022.7151559
TACATGG129650.022.6934172
ACATGGG130500.021.636513
AGAGTAC212750.021.30332210-11
GTACTTT133650.021.28794514-15
TACTTTT147850.020.6717914-15
CATGGGG52750.020.4213374
Produced by FastQC (version 0.11.5)