FastQCFastQC Report
Fri 27 May 2016
SRR939233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289840
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233711.020638996611117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147540.6443244942878106No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT140020.6114837717919156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116240.5076337211333543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71200.31093875554623907No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63800.2786220871327254No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55290.24145791845718478No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.19250253292806485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40300.17599482933305383No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38770.16931313978269225No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35140.15346050379065784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.14568703490200188No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31260.13651608846032912No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29240.12769451140691054No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29160.12734514201865632No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28870.12607867798623484No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.11865457848583306No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24060.105072843517451No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23730.10363169479090242No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.10144813611431366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG164950.028.1065121
TACATGG164250.027.799912
GACTGCG1257.532246E-626.537137
GTAAGCG906.6429493E-926.41374694-95
ACATGGG166100.026.2627753
CATGGGG49450.024.5463334
CATGGGA104100.023.8667624
GAGTACT149000.022.95911612-13
AGTACTT151750.022.37132512-13
GTACGCT1100.002908034521.5526053
GTATCAA332200.021.4696061
GTACTTT161650.021.13913314-15
ATGGGAG37100.020.9595955
TAAGGTG8150.020.9439145
ATGGGAT39200.020.9253625
GTATTAG8350.019.8905261
TATACGA1456.222547E-419.6203022
AGAGTAC260550.019.36659210-11
GTAAGGT7400.019.2221724
GGGCGTA4200.019.179919
Produced by FastQC (version 0.11.5)