##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939233_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2289840 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07796920308842 33.0 31.0 34.0 30.0 34.0 2 32.10934475771233 33.0 31.0 34.0 30.0 34.0 3 32.15761712608741 34.0 31.0 34.0 30.0 34.0 4 35.65373956258953 37.0 35.0 37.0 35.0 37.0 5 35.70513616671907 37.0 35.0 37.0 35.0 37.0 6 35.82197708136813 37.0 36.0 37.0 35.0 37.0 7 35.76887686475911 37.0 35.0 37.0 35.0 37.0 8 35.76279914753869 37.0 35.0 37.0 35.0 37.0 9 37.39065698913461 39.0 37.0 39.0 35.0 39.0 10-11 37.30356400447193 39.0 37.0 39.0 34.0 39.0 12-13 37.21979199420046 39.0 37.0 39.0 34.0 39.0 14-15 38.41676711036579 40.0 38.0 41.0 34.0 41.0 16-17 38.38366719945498 40.0 38.0 41.0 34.0 41.0 18-19 38.37942541836985 40.0 38.0 41.0 34.0 41.0 20-21 38.36463137162422 40.0 38.0 41.0 34.0 41.0 22-23 38.35207023198127 40.0 38.0 41.0 34.0 41.0 24-25 38.2567327848234 40.0 38.0 41.0 34.0 41.0 26-27 38.1472332128009 40.0 38.0 41.0 34.0 41.0 28-29 37.994193480767215 40.0 38.0 41.0 33.0 41.0 30-31 37.75185493309576 40.0 37.0 41.0 33.0 41.0 32-33 37.45425444572547 40.0 36.5 41.0 33.0 41.0 34-35 37.12036037452398 40.0 36.0 41.0 31.5 41.0 36-37 36.84112470740314 40.0 35.5 41.0 31.0 41.0 38-39 36.23775765992384 38.5 35.0 40.5 30.5 41.0 40-41 36.18339534639975 39.0 35.0 40.0 30.0 41.0 42-43 36.23628572301995 39.0 35.0 41.0 30.0 41.0 44-45 36.01056995248577 39.0 35.0 41.0 29.5 41.0 46-47 35.57068354120812 39.0 35.0 41.0 26.5 41.0 48-49 35.196233361282886 39.0 35.0 41.0 24.5 41.0 50-51 34.0023340058694 38.0 34.0 40.0 23.0 40.5 52-53 34.10535102889285 38.0 34.0 39.5 23.0 40.5 54-55 34.57703638682179 39.0 35.0 40.5 23.0 41.0 56-57 34.47406194319254 38.5 35.0 41.0 22.5 41.0 58-59 34.27036233972679 38.0 34.5 40.0 22.0 41.0 60-61 33.95809598050519 38.0 34.0 40.0 20.0 41.0 62-63 33.603450896132486 37.5 34.0 40.0 18.5 41.0 64-65 33.22652892778535 37.0 33.5 40.0 16.5 41.0 66-67 32.848256428396745 36.0 33.0 39.5 12.5 41.0 68-69 32.440021573559726 36.0 33.0 39.0 8.0 41.0 70-71 32.01174208678336 35.0 32.0 39.0 6.5 40.0 72-73 31.574824878594136 35.0 32.0 38.0 2.0 40.0 74-75 31.121310222548303 35.0 31.0 37.0 2.0 39.5 76-77 30.66190498026063 35.0 31.0 36.5 2.0 39.0 78-79 30.26474775530168 35.0 31.0 36.0 2.0 39.0 80-81 29.84358099255843 35.0 30.5 36.0 2.0 37.5 82-83 29.396261965901548 34.0 30.0 35.0 2.0 37.0 84-85 28.963037592146176 34.0 29.0 35.0 2.0 37.0 86-87 28.54940563532823 34.0 29.0 35.0 2.0 36.0 88-89 28.08280971596269 34.0 28.5 35.0 2.0 36.0 90-91 27.643008245117564 34.0 27.0 35.0 2.0 35.5 92-93 27.19135943996087 34.0 26.5 35.0 2.0 35.0 94-95 26.682681104356636 34.0 25.0 35.0 2.0 35.0 96-97 26.073456660727388 33.0 24.5 35.0 2.0 35.0 98-99 25.34118606540195 33.0 19.0 35.0 2.0 35.0 100-101 22.741827813297 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35609186738314236 1101 2 0.4387477833021052 1101 3 0.4672626600259093 1101 4 0.39297871249315364 1101 5 0.2531939468590281 1101 6 0.34614088024658685 1101 7 0.31056720986905617 1101 8 0.24227699542245773 1101 9 0.3138575680086575 1101 10-11 0.4831806582189273 1101 12-13 0.46822711300343656 1101 14-15 0.6009429395195625 1101 16-17 0.7195293919800747 1101 18-19 0.7026779727244588 1101 20-21 0.6304830806458455 1101 22-23 0.6756034546047474 1101 24-25 0.6738082209541929 1101 26-27 0.5620545739334233 1101 28-29 0.6648572030692819 1101 30-31 0.7617329967687922 1101 32-33 0.767024730851297 1101 34-35 0.7250776069036391 1101 36-37 0.5727027770331645 1101 38-39 0.9667714007821999 1101 40-41 0.7401575270170397 1101 42-43 0.7219587415633484 1101 44-45 0.653254954478065 1101 46-47 0.61323075916021 1101 48-49 0.4381580284878268 1101 50-51 0.4559062406097496 1101 52-53 0.4055533086631016 1101 54-55 0.4819871617095117 1101 56-57 0.5212477454092763 1101 58-59 0.5460441209195182 1101 60-61 0.5865584991004482 1101 62-63 0.6735522170079946 1101 64-65 0.5156457251784019 1101 66-67 0.4511808690125747 1101 68-69 0.6160911490454808 1101 70-71 0.6373350044882642 1101 72-73 0.6607071614354716 1101 74-75 0.5458043969995288 1101 76-77 0.6131751393360467 1101 78-79 0.6698898002243787 1101 80-81 0.49506929701501434 1101 82-83 0.5780378348929922 1101 84-85 0.6179820815304389 1101 86-87 0.5424057354486038 1101 88-89 0.5776362980140881 1101 90-91 0.6316653678265993 1101 92-93 0.6642360170188795 1101 94-95 0.6791788182668554 1101 96-97 0.6350358257201556 1101 98-99 0.5710041389348284 1101 100-101 0.3231539681424671 1102 1 0.3668214519347188 1102 2 0.36660435953564985 1102 3 0.366997706829018 1102 4 0.3120322244262468 1102 5 0.33316629562719413 1102 6 0.37699036235260763 1102 7 0.3338368547093822 1102 8 0.3483992501311022 1102 9 0.4986541501350814 1102 10-11 0.5601332431558106 1102 12-13 0.4271893985364059 1102 14-15 0.554271966513511 1102 16-17 0.5811917523073618 1102 18-19 0.5495957669720966 1102 20-21 0.5566896489466515 1102 22-23 0.6434429188054693 1102 24-25 0.6490700010589094 1102 26-27 0.5888258393172521 1102 28-29 0.5743259367788482 1102 30-31 0.6013036094022297 1102 32-33 0.4675179200355828 1102 34-35 0.5523567936854477 1102 36-37 0.6840949712264006 1102 38-39 1.0927879235264726 1102 40-41 0.6723465073212012 1102 42-43 0.6710247192157155 1102 44-45 0.7973698884097544 1102 46-47 0.6538192544345591 1102 48-49 0.6260884924392371 1102 50-51 0.6458223803164671 1102 52-53 0.7429922833010281 1102 54-55 0.7920347580921856 1102 56-57 0.6975796508511252 1102 58-59 0.7260325617980072 1102 60-61 0.6469733809027645 1102 62-63 0.572025733020773 1102 64-65 0.6989988903378546 1102 66-67 0.6207038852633389 1102 68-69 0.5394139431797953 1102 70-71 0.5760866193655332 1102 72-73 0.6241700249245135 1102 74-75 0.5640363047078765 1102 76-77 0.5068940033690446 1102 78-79 0.48808827714013603 1102 80-81 0.4406394336846269 1102 82-83 0.36362270377629 1102 84-85 0.2900042478458005 1102 86-87 0.24440829658729513 1102 88-89 0.14537966285169546 1102 90-91 0.2067305295409767 1102 92-93 0.08671782840121267 1102 94-95 0.11070394235742143 1102 96-97 0.21584723072004408 1102 98-99 0.24762524906379113 1102 100-101 -0.07691402668992708 1103 1 0.27832460153641136 1103 2 0.3796055568751129 1103 3 0.31201510404987687 1103 4 0.2567630076681766 1103 5 0.24577042115735992 1103 6 0.2980834851030991 1103 7 0.28902195600591796 1103 8 0.3238663993959676 1103 9 0.3586100120326208 1103 10-11 0.37484732488560013 1103 12-13 0.39108267907092653 1103 14-15 0.6178398174760389 1103 16-17 0.6144852418697013 1103 18-19 0.6117318989306995 1103 20-21 0.6321103275710982 1103 22-23 0.6583517989756089 1103 24-25 0.7397259125341407 1103 26-27 0.7502996070460028 1103 28-29 0.6429145982127693 1103 30-31 0.5621729210828832 1103 32-33 0.5770089629547286 1103 34-35 0.48812869488850197 1103 36-37 0.28603768715614564 1103 38-39 0.686567994916544 1103 40-41 0.7145693844752543 1103 42-43 0.6625106179430418 1103 44-45 0.7135655820403457 1103 46-47 0.7238598982330515 1103 48-49 0.6547080127199365 1103 50-51 0.5077952346179515 1103 52-53 0.601120952939354 1103 54-55 0.6008329516811273 1103 56-57 0.6561231790264301 1103 58-59 0.679052635583659 1103 60-61 0.7242327343574644 1103 62-63 0.5539684894772492 1103 64-65 0.6273533883761289 1103 66-67 0.6154902351431346 1103 68-69 0.5714443499284911 1103 70-71 0.44761882662638897 1103 72-73 0.6407434275975668 1103 74-75 0.5825940532593847 1103 76-77 0.5544460317989923 1103 78-79 0.5211149657872909 1103 80-81 0.5016918135713055 1103 82-83 0.3993213416731827 1103 84-85 0.5602053549457651 1103 86-87 0.5214517777065666 1103 88-89 0.5230494169040298 1103 90-91 0.5711008771296591 1103 92-93 0.7547658183433832 1103 94-95 0.6479016186452853 1103 96-97 0.7483218553638018 1103 98-99 0.6845960657717889 1103 100-101 0.26515964458522845 1104 1 0.327918369571762 1104 2 0.3798062791238337 1104 3 0.33612988379564257 1104 4 0.358297720319122 1104 5 0.30109685175357725 1104 6 0.31457038018026395 1104 7 0.33054598689518855 1104 8 0.42240433326567484 1104 9 0.5659055849870356 1104 10-11 0.4980732169137241 1104 12-13 0.5242192870318192 1104 14-15 0.5248015414561635 1104 16-17 0.5521855798033997 1104 18-19 0.5899627585050595 1104 20-21 0.6378368410915272 1104 22-23 0.6994003772735411 1104 24-25 0.7916587316320047 1104 26-27 0.6914915019079473 1104 28-29 0.7499852041304251 1104 30-31 0.7288380504894434 1104 32-33 0.6975049006350886 1104 34-35 0.6861420716867741 1104 36-37 0.5619739005243432 1104 38-39 1.157383550934746 1104 40-41 1.0544883401222265 1104 42-43 0.9751302497209267 1104 44-45 0.9990839768948305 1104 46-47 0.9794921996695578 1104 48-49 0.8691992806432864 1104 50-51 0.8404214283004308 1104 52-53 0.8251454296340484 1104 54-55 1.0512257875219504 1104 56-57 0.9975987203396315 1104 58-59 0.9185643490664859 1104 60-61 0.9578723338265362 1104 62-63 0.9750444573581731 1104 64-65 0.9205435896166563 1104 66-67 1.0324549106626648 1104 68-69 1.0388693689685553 1104 70-71 1.156427722355346 1104 72-73 1.0763133426266087 1104 74-75 1.1314510344846056 1104 76-77 1.0757935237371612 1104 78-79 1.1475546098808316 1104 80-81 1.0074714723698364 1104 82-83 0.9458399306616592 1104 84-85 1.1138515602411445 1104 86-87 1.0417086934005866 1104 88-89 0.9985999536713415 1104 90-91 1.1149257471872573 1104 92-93 1.1385867266620657 1104 94-95 1.14726211843929 1104 96-97 1.1995098938989592 1104 98-99 1.253163576526024 1104 100-101 1.2139087101506902 1105 1 0.24557338722261335 1105 2 0.3312051160313061 1105 3 0.26687148044578635 1105 4 0.3135327936634553 1105 5 0.23839730414213278 1105 6 0.28052217784944844 1105 7 0.23633932025376225 1105 8 0.31841007846990976 1105 9 0.4119840918397557 1105 10-11 0.44352490919526133 1105 12-13 0.4054425746298662 1105 14-15 0.504675867217145 1105 16-17 0.48288699872916396 1105 18-19 0.38915217817464764 1105 20-21 0.46497333891498016 1105 22-23 0.4608714331146828 1105 24-25 0.39568180500494776 1105 26-27 0.3929869967439572 1105 28-29 0.5032974473137841 1105 30-31 0.5027165555751836 1105 32-33 0.533346607132394 1105 34-35 0.48907948404779233 1105 36-37 0.4001812546045258 1105 38-39 0.8373633484810838 1105 40-41 0.7032785488739037 1105 42-43 0.5466869871887425 1105 44-45 0.5039780865038708 1105 46-47 0.6345619132802156 1105 48-49 0.5290433069308733 1105 50-51 0.6264896656426586 1105 52-53 0.5218394760525769 1105 54-55 0.6409676782477902 1105 56-57 0.7358588313479473 1105 58-59 0.6057004093524228 1105 60-61 0.7105677122102492 1105 62-63 0.6804102660726628 1105 64-65 0.651157732428068 1105 66-67 0.6199442193912432 1105 68-69 0.5687089020823564 1105 70-71 0.570698976266371 1105 72-73 0.5427624185179134 1105 74-75 0.4070008066916344 1105 76-77 0.508851019112516 1105 78-79 0.5066569048913472 1105 80-81 0.5654298381503615 1105 82-83 0.4241989672208639 1105 84-85 0.5023441761643959 1105 86-87 0.5635157317806261 1105 88-89 0.49027001756443767 1105 90-91 0.4838057436869576 1105 92-93 0.551580181689733 1105 94-95 0.5377508941293705 1105 96-97 0.594922334016271 1105 98-99 0.6154816671459322 1105 100-101 0.3842841383800213 1106 1 0.30920542049669564 1106 2 0.37248783635250504 1106 3 0.32872154596755365 1106 4 0.21698022821880159 1106 5 0.2202799642898725 1106 6 0.26611940746649054 1106 7 0.17710251364573537 1106 8 0.22856473067571415 1106 9 0.219575643037075 1106 10-11 0.3265651472930813 1106 12-13 0.4462648798343096 1106 14-15 0.5854585388627456 1106 16-17 0.5356762910580173 1106 18-19 0.5486701414752488 1106 20-21 0.5151073069942029 1106 22-23 0.5760348048636885 1106 24-25 0.4773228098136144 1106 26-27 0.5156007404106191 1106 28-29 0.5466419700264566 1106 30-31 0.4849570715445921 1106 32-33 0.45281732905768735 1106 34-35 0.4958108391886 1106 36-37 0.5681360219491651 1106 38-39 1.0683344928625402 1106 40-41 0.7986039082220415 1106 42-43 0.7000729262292609 1106 44-45 0.6675109858030552 1106 46-47 0.5901456259026574 1106 48-49 0.5322732236375032 1106 50-51 0.366930618530688 1106 52-53 0.6037868567242413 1106 54-55 0.395965941550358 1106 56-57 0.50054924850388 1106 58-59 0.4623684862093995 1106 60-61 0.5164568191711751 1106 62-63 0.36464101326920684 1106 64-65 0.4879932052845035 1106 66-67 0.49569583680340656 1106 68-69 0.577626452822166 1106 70-71 0.547979187891066 1106 72-73 0.621205456244116 1106 74-75 0.6963121650935022 1106 76-77 0.7254386229788388 1106 78-79 0.8885768028768979 1106 80-81 0.9288194549031026 1106 82-83 0.810680249949197 1106 84-85 0.9899133686341415 1106 86-87 1.0236151582097541 1106 88-89 0.9254555477298929 1106 90-91 0.8910944444970568 1106 92-93 0.9813245606591465 1106 94-95 1.1952063539495619 1106 96-97 1.3015395518790562 1106 98-99 1.3966514800994112 1106 100-101 0.9502791418193617 1107 1 0.15079442925895847 1107 2 0.08686473220932811 1107 3 0.16144271502663088 1107 4 0.1133781914336609 1107 5 0.08925415817760296 1107 6 0.11878380975881697 1107 7 0.20530161426911064 1107 8 0.16952840662523982 1107 9 0.22627470507624992 1107 10-11 0.24024029058251983 1107 12-13 0.21629123226816915 1107 14-15 0.3528708596237706 1107 16-17 0.3537376710363773 1107 18-19 0.3329341603058751 1107 20-21 0.24332719701578753 1107 22-23 0.21052963784399736 1107 24-25 0.33406657961680963 1107 26-27 0.33084528170589067 1107 28-29 0.396790912723354 1107 30-31 0.3374537957266668 1107 32-33 0.48389258437568117 1107 34-35 0.5075591527134549 1107 36-37 0.43814133543524036 1107 38-39 0.7177158211035177 1107 40-41 0.5464522151050417 1107 42-43 0.4295083145840479 1107 44-45 0.3924871154275493 1107 46-47 0.5659511239018471 1107 48-49 0.45114398882941487 1107 50-51 0.4536497635508425 1107 52-53 0.4992289491320534 1107 54-55 0.5189954118180609 1107 56-57 0.4455146436579369 1107 58-59 0.43992601410231913 1107 60-61 0.36103888700028364 1107 62-63 0.42806806590073876 1107 64-65 0.3003504581354193 1107 66-67 0.2907705346660663 1107 68-69 0.28128509896587417 1107 70-71 0.3085812050961607 1107 72-73 0.21468096372234413 1107 74-75 0.3273777071746622 1107 76-77 0.3414759064514037 1107 78-79 0.31952288750184366 1107 80-81 0.28968501564289184 1107 82-83 0.3525942106347735 1107 84-85 0.30359649750959505 1107 86-87 0.5642026669871782 1107 88-89 0.38082706368205876 1107 90-91 0.43106470202836533 1107 92-93 0.46395811862548086 1107 94-95 0.6341332255616869 1107 96-97 0.6347347691273093 1107 98-99 0.8679790991316771 1107 100-101 0.6335317362709922 1108 1 0.12022197051367556 1108 2 0.08882324962704047 1108 3 0.05322734748141045 1108 4 0.0766499152758442 1108 5 0.21358653447929754 1108 6 0.1267293548504398 1108 7 0.2282840992732602 1108 8 0.22905553939023804 1108 9 0.26818742297498943 1108 10-11 0.3483018925306851 1108 12-13 0.2678523240447106 1108 14-15 0.2827339934037383 1108 16-17 0.26800491119246317 1108 18-19 0.26769414407980463 1108 20-21 0.3275612523673246 1108 22-23 0.3006791243429561 1108 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-1.2864634793289795 2316 58-59 -1.1694979848928568 2316 60-61 -1.177355550083874 2316 62-63 -1.5132473238455972 2316 64-65 -1.5100847350115174 2316 66-67 -1.5977260494889372 2316 68-69 -1.6492632980657191 2316 70-71 -1.4540319995814457 2316 72-73 -1.3214937846843924 2316 74-75 -1.4104622557536075 2316 76-77 -1.410847261443113 2316 78-79 -1.3274330631795657 2316 80-81 -1.2329647215595436 2316 82-83 -1.247268784467682 2316 84-85 -1.2810140574657005 2316 86-87 -1.2059839476708092 2316 88-89 -1.343736793223865 2316 90-91 -1.3777593078142942 2316 92-93 -1.4350429144222154 2316 94-95 -1.4723280167534938 2316 96-97 -1.386447704141748 2316 98-99 -1.6007827058249866 2316 100-101 -0.6800757094115362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7506.0 3 1574.0 4 1462.0 5 1611.0 6 2045.0 7 2367.0 8 3048.0 9 3774.0 10 4501.0 11 6497.0 12 9723.0 13 14194.0 14 15325.0 15 17664.0 16 23991.0 17 32426.0 18 26995.0 19 15648.0 20 14296.0 21 14868.0 22 15657.0 23 17088.0 24 19587.0 25 22180.0 26 25134.0 27 29512.0 28 34264.0 29 40906.0 30 49447.0 31 60245.0 32 75529.0 33 97246.0 34 133844.0 35 202506.0 36 293611.0 37 445167.0 38 434675.0 39 73708.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.377594756745246 19.439444069233808 14.006277259344715 21.176683914676225 2 15.091276275343462 21.930543755124255 39.77791466764201 23.200265301890276 3 19.791573697335608 27.225576409852565 30.07673797961237 22.906111913199457 4 12.054672833806663 16.66917553156575 38.626746541923566 32.649405092704015 5 12.366407402933655 39.35233083522836 33.38094963001087 14.900312131827118 6 29.321986177758504 37.137393500188864 18.18711614600092 15.353504176051707 7 27.004400952337228 35.16472416982035 21.083644329592634 16.747230548249785 8 26.267549738380165 32.876243869480895 20.719897319954608 20.136309072184332 9 27.62494074181625 17.1779634777163 21.681804107751056 33.515291672716394 10-11 24.51646869484208 27.125459601525996 27.122201915737882 21.235869787894046 12-13 26.614837778948086 25.311573420358997 27.784485568557958 20.28910323213496 14-15 22.92818519108862 25.95461223090464 28.32293782746281 22.794264750543935 16-17 20.49058453155241 29.621444525615416 28.494093390919545 21.393877551912627 18-19 20.190438623688678 27.630611692462704 32.417422151631094 19.761527532217528 20-21 21.55643020739105 26.21936069735744 31.97554279835319 20.24866629689832 22-23 20.709134728424424 26.549388144868015 32.055417240165795 20.68605988654177 24-25 20.84092472316586 26.70443109643297 32.34990925335728 20.1047349270439 26-27 20.51396294658266 26.550377677833616 32.883103384467596 20.05255599111614 28-29 19.749369201642 27.589051055296782 32.7203026082331 19.941277134828116 30-31 20.169654920832514 26.92026914189261 32.32217769960927 20.587898237665605 32-33 19.832689630011664 27.283571982566862 32.274405279810814 20.60933310761066 34-35 20.88803197095653 27.023285349727256 32.081175517273266 20.00750716204295 36-37 20.866364405028257 26.49616658936807 32.22848996573559 20.408979039868086 38-39 20.241925667784805 27.32126154004735 32.16174872496115 20.275064067206692 40-41 20.315460789105906 27.42752772238819 32.101590242193026 20.155421246312873 42-43 19.998218915859347 27.78028440755153 31.17717597703542 21.044320699553698 44-45 20.308503646977172 27.41554657277152 31.16926133028461 21.1066884499667 46-47 20.976470402423157 28.072750820192844 30.41733096656842 20.533447810815577 48-49 20.84686223164516 28.08228524592466 30.148038324370617 20.922814198059562 50-51 20.254774582912958 28.411913582237514 30.521814270210935 20.811497564638593 52-53 20.96972526396085 28.826255874907336 29.54707352116217 20.65694533996964 54-55 20.13472370891607 28.126590274974994 30.155101163423236 21.5835848526857 56-57 20.791177612061386 28.467812843288375 29.466218779258313 21.274790765391927 58-59 21.20247260306258 27.87104666879331 29.63054557094264 21.295935157201473 60-61 20.657423810646655 28.26044938285024 30.496187354763155 20.58593945173995 62-63 20.486347553827038 28.21817061784383 30.370308781511717 20.925173046817413 64-65 20.072504384815105 28.60077506200167 30.137187948696294 21.189532604486935 66-67 20.380049767638397 28.404280666312413 29.44531154357345 21.770358022475737 68-69 20.381073960041146 28.215104461053986 29.84995242993509 21.553869148969785 70-71 20.644696009632767 28.397227068640497 29.654090064979062 21.303986856747677 72-73 20.790596265464764 27.906361943474746 29.98836797054214 21.314673820518358 74-75 19.974290616952793 29.217009089115987 29.366684299175777 21.442015994755444 76-77 20.336537870984383 28.34883982108169 29.460191728274392 21.854430579659535 78-79 20.37768953836605 28.439664748720546 29.579948964429718 21.602696748483684 80-81 20.653308375633124 28.6161306134265 29.207088600932295 21.523472410008083 82-83 20.595227728805217 28.32865663594954 29.366661921608273 21.709453713636968 84-85 20.21670642988451 28.298857376864717 29.375151209533694 22.109284983717078 86-87 20.432354060489207 28.454209754562136 29.119531826641616 21.99390435830704 88-89 20.39249992761056 28.21481500330359 28.87004911075058 22.52263595833527 90-91 20.333628503426198 27.929819141765893 29.187217409009214 22.549334945798698 92-93 20.893801820532698 27.98557247474975 29.21769138093814 21.902934323779416 94-95 20.55181792391292 27.48566765817576 29.473951658501363 22.48856275940996 96-97 20.239615130866607 27.362818406826612 29.769588852889623 22.627977609417158 98-99 20.433747627771258 27.618666286378748 28.826226702794017 23.121359383055978 100-101 20.523218102159166 26.7770390517223 29.720807177583147 22.97893566853538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5902.0 1 3780.0 2 2191.0 3 2802.0 4 2360.5 5 2203.5 6 3985.0 7 7598.0 8 11415.5 9 11854.5 10 9102.5 11 7174.5 12 6869.5 13 6897.5 14 7100.0 15 7268.5 16 7179.5 17 7241.0 18 7467.5 19 7639.5 20 8096.0 21 8968.5 22 10111.5 23 11551.5 24 12990.5 25 15072.0 26 18791.0 27 22963.0 28 25767.5 29 28747.5 30 33470.0 31 38652.5 32 45807.0 33 51824.0 34 56309.5 35 59991.0 36 63815.5 37 68674.0 38 68712.5 39 66164.5 40 64812.0 41 66329.5 42 67387.0 43 68492.0 44 69467.5 45 69918.0 46 70503.5 47 77339.5 48 101529.0 49 101291.5 50 77064.5 51 65111.0 52 58221.5 53 56896.0 54 56808.0 55 52479.5 56 46576.0 57 41459.0 58 39291.5 59 36159.0 60 31326.5 61 27592.0 62 23849.0 63 18345.0 64 13001.5 65 10289.5 66 7620.5 67 5987.0 68 4769.5 69 3388.0 70 2692.0 71 2295.5 72 2013.0 73 1883.0 74 1621.5 75 1446.5 76 1128.0 77 635.0 78 378.5 79 231.0 80 150.5 81 133.0 82 112.0 83 83.5 84 52.0 85 29.5 86 21.5 87 14.5 88 10.5 89 12.0 90 10.0 91 4.0 92 2.5 93 3.0 94 4.5 95 3.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2916360968451944 2 0.18088600076861264 3 0.1807986584215491 4 0.20132410998148342 5 0.21433811969395244 6 0.10970198791181918 7 0.12380777696258252 8 0.13647241728679732 9 0.14131991754882436 10-11 0.12841508577018482 12-13 0.11784666177549524 14-15 0.13252017608217168 16-17 0.1086102085735248 18-19 0.1362103902456067 20-21 0.16263145023233064 22-23 0.16597229500751146 24-25 0.19023163190441253 26-27 0.17018656325332773 28-29 0.16804667575027074 30-31 0.15769660762324006 32-33 0.16809034692380254 34-35 0.35019914055130485 36-37 0.47160500296963986 38-39 0.43703926911923974 40-41 0.4270385703804632 42-43 0.45110138699647134 44-45 0.4549662858540335 46-47 0.4584599797365755 48-49 0.47025119659015474 50-51 0.46332931558536844 52-53 0.4699673339621982 54-55 0.4524333577891905 56-57 0.4150945044195228 58-59 0.4038491772350906 60-61 0.35906438877825525 62-63 0.4667575027076128 64-65 0.46586224365021134 66-67 0.47378856164622857 68-69 0.484575341508577 70-71 0.4782866925200014 72-73 0.47112462006079026 74-75 0.47625598295077387 76-77 0.45260804248331765 78-79 0.43922282779582855 80-81 0.4359474897809454 82-83 0.4487431436257555 84-85 0.450009607658177 86-87 0.45166911225238443 88-89 0.4586346644307026 90-91 0.4772385843552388 92-93 0.4804484156098242 94-95 0.4799680327009747 96-97 0.4775661181567271 98-99 0.47448730042273696 100-101 0.4570406665967928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2289840.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.83796291896264 #Duplication Level Percentage of deduplicated Percentage of total 1 70.66489354447697 27.44480515152839 2 14.257128456277988 11.07435652631623 3 5.595667732520903 6.5197300770744775 4 2.8343112453115857 4.403155001848407 5 1.694692892647609 3.290920986183869 6 1.0451628639531552 2.435519793269366 7 0.6982452460573949 1.8982896082303329 8 0.49222788648010013 1.5293702722234783 9 0.3714570056217889 1.298397006929514 >10 1.9800856771856161 14.306640032198944 >50 0.20608055025227134 5.5476082659853665 >100 0.14502726937944274 10.578512964345324 >500 0.010050729739390071 2.662449263296783 >1k 0.004178393262443064 3.379552674190668 >5k 3.3878864290078894E-4 0.8346031073944795 >10k+ 4.5171819053438524E-4 2.7960892689844687 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23371 1.020638996611117 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14754 0.6443244942878106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14002 0.6114837717919156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11624 0.5076337211333543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7120 0.31093875554623907 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6380 0.2786220871327254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5529 0.24145791845718478 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4408 0.19250253292806485 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4030 0.17599482933305383 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3877 0.16931313978269225 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3514 0.15346050379065784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3336 0.14568703490200188 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3126 0.13651608846032912 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2924 0.12769451140691054 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2916 0.12734514201865632 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2887 0.12607867798623484 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2717 0.11865457848583306 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2406 0.105072843517451 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2373 0.10363169479090242 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.10144813611431366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.550676029766272E-5 0.0 18-19 0.0 0.0 0.0 1.9652028089298817E-4 0.0 20-21 0.0 0.0 0.0 2.620270411906509E-4 0.0 22-23 0.0 0.0 0.0 9.826014044649408E-4 0.0 24-25 0.0 0.0 0.0 0.0019652028089298815 0.0 26-27 0.0 0.0 0.0 0.0028822974530971594 0.0 28-29 0.0 0.0 0.0 0.007424099500401775 4.367117353177515E-5 30-31 0.0 0.0 0.0 0.021202354749676834 4.367117353177515E-5 32-33 0.0 0.0 0.0 0.04554903399364148 4.367117353177515E-5 34-35 0.0 0.0 0.0 0.08343377703245641 4.367117353177515E-5 36-37 0.0 0.0 0.0 0.13959490619431925 4.367117353177515E-5 38-39 0.0 0.0 0.0 0.2298413862977326 4.367117353177515E-5 40-41 0.0 0.0 0.0 0.3368575970373476 4.367117353177515E-5 42-43 0.0 0.0 0.0 0.4443978618593439 4.367117353177515E-5 44-45 0.0 0.0 0.0 0.5746034657443315 4.367117353177515E-5 46-47 0.0 0.0 0.0 0.7381083394472976 4.367117353177515E-5 48-49 0.0 0.0 0.0 0.906417042238759 4.367117353177515E-5 50-51 0.0 0.0 0.0 1.0952511965901548 4.367117353177515E-5 52-53 0.0 0.0 0.0 1.2830809139503196 4.367117353177515E-5 54-55 0.0 0.0 0.0 1.4918509590189708 4.367117353177515E-5 56-57 0.0 0.0 0.0 1.7428073577193168 4.367117353177515E-5 58-59 0.0 0.0 0.0 2.013481291269259 4.367117353177515E-5 60-61 0.0 0.0 0.0 2.31708328966216 4.367117353177515E-5 62-63 0.0 0.0 0.0 2.6266027320686165 4.367117353177515E-5 64-65 0.0 0.0 0.0 2.946079202040317 4.367117353177515E-5 66-67 0.0 0.0 0.0 3.3156028368794326 4.367117353177515E-5 68-69 0.0 0.0 0.0 3.736003388883066 4.367117353177515E-5 70-71 0.0 0.0 0.0 4.195773503825595 4.367117353177515E-5 72-73 0.0 0.0 0.0 4.658469587394753 4.367117353177515E-5 74-75 0.0 0.0 0.0 5.14367816091954 4.367117353177515E-5 76-77 0.0 0.0 0.0 5.6667059707228455 4.367117353177515E-5 78-79 0.0 0.0 0.0 6.27288369493065 4.367117353177515E-5 80-81 0.0 0.0 0.0 6.930003843063271 4.367117353177515E-5 82-83 0.0 0.0 0.0 7.577822031233623 4.367117353177515E-5 84-85 0.0 0.0 0.0 8.24542326101387 4.367117353177515E-5 86-87 0.0 0.0 0.0 8.94634559619886 4.367117353177515E-5 88-89 0.0 0.0 0.0 9.688930230933167 4.367117353177515E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16495 0.0 28.106512 1 TACATGG 16425 0.0 27.79991 2 GACTGCG 125 7.532246E-6 26.53713 7 GTAAGCG 90 6.6429493E-9 26.413746 94-95 ACATGGG 16610 0.0 26.262775 3 CATGGGG 4945 0.0 24.546333 4 CATGGGA 10410 0.0 23.866762 4 GAGTACT 14900 0.0 22.959116 12-13 AGTACTT 15175 0.0 22.371325 12-13 GTACGCT 110 0.0029080345 21.552605 3 GTATCAA 33220 0.0 21.469606 1 GTACTTT 16165 0.0 21.139133 14-15 ATGGGAG 3710 0.0 20.959595 5 TAAGGTG 815 0.0 20.943914 5 ATGGGAT 3920 0.0 20.925362 5 GTATTAG 835 0.0 19.890526 1 TATACGA 145 6.222547E-4 19.620302 2 AGAGTAC 26055 0.0 19.366592 10-11 GTAAGGT 740 0.0 19.222172 4 GGGCGTA 420 0.0 19.17991 9 >>END_MODULE