FastQCFastQC Report
Fri 27 May 2016
SRR939233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289840
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231321.0102015861370228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154800.6760297662718793No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152130.6643695629388953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127150.5552789714565209No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65240.284910736121301No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56960.24875100443699122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52670.2300160709918597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.1904936589456032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43430.18966390664849947No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40960.178877126786151No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35810.15638647241728681No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.15625545889669148No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34500.15066554868462426No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32450.14171295811061033No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31480.13747685427802817No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27980.12219194354190685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.11061908255598643No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25100.10961464556475563No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24850.10852286622646125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.10105509555252767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1203.7343852E-935.6859741
GTAAGCG1850.026.93484194-95
GTACATG158300.026.5409281
TACATGG158050.026.2129922
ACATGGG157550.025.000953
GAGTACG2602.3646862E-1123.790651
CATGGGG47500.023.378634
GAGTACT165900.023.27189312-13
ATCGATC1050.002208576822.5983928
CATGGGA98750.022.5388934
AGTACTT168200.022.24827212-13
GTACTTT179050.021.56296714-15
ATGGGAG34550.021.4275935
TAGTCGA1805.2135365E-621.0918335
GTATCAA343750.020.9288521
CGGTATA2056.895607E-720.8346162
ATGGGAT36050.020.141095
GTACCGT1200.004790164519.7735946
GCACCGT4800.019.7735946
AGAGTAC275200.019.41805610-11
Produced by FastQC (version 0.11.5)