##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939233_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2289840 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27363702267407 33.0 31.0 34.0 31.0 34.0 2 32.46766149599972 34.0 31.0 34.0 31.0 34.0 3 32.55086512594767 34.0 31.0 34.0 31.0 34.0 4 36.080626593997835 37.0 35.0 37.0 35.0 37.0 5 36.03868916605527 37.0 35.0 37.0 35.0 37.0 6 36.0895835516892 37.0 35.0 37.0 35.0 37.0 7 36.03116855326137 37.0 35.0 37.0 35.0 37.0 8 36.01199734479265 37.0 35.0 37.0 35.0 37.0 9 37.68734322048702 39.0 37.0 39.0 35.0 39.0 10-11 37.6167994270342 39.0 37.0 39.0 35.0 39.0 12-13 37.546794535862766 39.0 37.0 39.0 35.0 39.0 14-15 38.762508515878835 40.0 38.0 41.0 34.5 41.0 16-17 38.79059890647382 40.0 38.0 41.0 34.5 41.0 18-19 38.94444196974462 40.0 38.0 41.0 35.0 41.0 20-21 38.94036177200154 40.0 38.0 41.0 35.0 41.0 22-23 38.845071489711074 40.0 38.0 41.0 35.0 41.0 24-25 38.76236330922684 40.0 38.0 41.0 34.0 41.0 26-27 38.57319113999232 40.0 38.0 41.0 34.0 41.0 28-29 38.39345107081718 40.0 38.0 41.0 34.0 41.0 30-31 38.120676117108616 40.0 37.5 41.0 33.5 41.0 32-33 37.93171313279531 40.0 37.0 41.0 33.0 41.0 34-35 37.76245763896168 40.0 36.5 41.0 33.0 41.0 36-37 37.5503220749048 40.0 36.0 41.0 33.0 41.0 38-39 37.3702387503057 40.0 36.0 41.0 33.0 41.0 40-41 37.1515920326311 40.0 36.0 41.0 32.0 41.0 42-43 36.877960032141985 40.0 35.0 41.0 31.0 41.0 44-45 36.50685244383887 40.0 35.0 41.0 30.5 41.0 46-47 36.01564847325578 39.0 35.0 41.0 29.5 41.0 48-49 35.55175885651399 39.0 35.0 41.0 27.0 41.0 50-51 35.251661469797014 39.0 35.0 41.0 26.5 41.0 52-53 34.99951372148273 39.0 35.0 40.0 26.0 41.0 54-55 34.74768237082067 38.0 35.0 40.0 24.5 41.0 56-57 34.42179606435384 38.0 34.0 40.0 23.5 41.0 58-59 34.119468827516336 38.0 34.0 40.0 23.0 41.0 60-61 33.76272163120567 37.5 33.5 40.0 22.0 41.0 62-63 33.43899246235545 37.0 33.0 40.0 22.0 41.0 64-65 33.159226408832055 36.5 33.0 39.5 20.0 41.0 66-67 32.74697424274186 36.0 33.0 39.0 18.5 40.5 68-69 32.285304868462426 35.0 32.0 39.0 16.5 40.0 70-71 31.80880367187227 35.0 31.5 38.0 12.0 40.0 72-73 31.332138708381372 35.0 31.0 37.0 7.5 39.5 74-75 30.841351797505503 35.0 31.0 37.0 6.0 39.0 76-77 28.92478819480837 32.5 28.0 35.0 2.0 37.5 78-79 29.851328040387102 34.0 30.0 36.0 2.0 38.0 80-81 29.842663897914264 34.0 30.0 35.5 2.0 37.0 82-83 29.494067489431576 34.0 30.0 35.0 2.0 37.0 84-85 29.057206617056213 34.0 29.0 35.0 2.0 36.5 86-87 28.48895119309646 34.0 29.0 35.0 2.0 36.0 88-89 27.894462058484436 34.0 27.5 35.0 2.0 35.5 90-91 27.40350330154072 33.0 26.5 35.0 2.0 35.0 92-93 27.05906198686371 33.0 26.0 35.0 2.0 35.0 94-95 26.55978037766831 33.0 25.0 35.0 2.0 35.0 96-97 25.862199542326103 32.5 24.0 34.0 2.0 35.0 98-99 24.936461717849284 32.0 11.0 34.0 2.0 35.0 100-101 20.841521460014675 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2685386615947394 1101 2 0.3217728785507532 1101 3 0.31526216130707496 1101 4 0.15796772233598944 1101 5 0.22750456675138508 1101 6 0.22288888592706257 1101 7 0.22921339009228348 1101 8 0.24985956337751247 1101 9 0.34436308284726636 1101 10-11 0.33449577547243337 1101 12-13 0.29851883755063113 1101 14-15 0.39472328771102383 1101 16-17 0.3543514284685827 1101 18-19 0.39984518142000525 1101 20-21 0.48616478462118806 1101 22-23 0.43010711423626447 1101 24-25 0.5085916067919882 1101 26-27 0.4914496818656957 1101 28-29 0.44591060493907264 1101 30-31 0.45298851855244493 1101 32-33 0.6319046680741494 1101 34-35 0.6222018391133659 1101 36-37 0.5203594900410664 1101 38-39 0.5418357564833371 1101 40-41 0.6359390191278891 1101 42-43 0.5079050145302801 1101 44-45 0.590726538750161 1101 46-47 0.5948327490534737 1101 48-49 0.4353943931911388 1101 50-51 0.4454135882279431 1101 52-53 0.4153058581805382 1101 54-55 0.3587260982861622 1101 56-57 0.4230405576264431 1101 58-59 0.4578235116787184 1101 60-61 0.36824965956284217 1101 62-63 0.3600154731382972 1101 64-65 0.28663503041187255 1101 66-67 0.21459719578964354 1101 68-69 0.3388709050542573 1101 70-71 0.29172704730524757 1101 72-73 0.3973604605802983 1101 74-75 0.27804304568916294 1101 76-77 0.3867651621911854 1101 78-79 0.42465173480082896 1101 80-81 0.49419832796041163 1101 82-83 0.4572882899616104 1101 84-85 0.4365902550833596 1101 86-87 0.44921529564968665 1101 88-89 0.6213698040228621 1101 90-91 0.6154821097647236 1101 92-93 0.558253435142845 1101 94-95 0.49311346547640156 1101 96-97 0.49308221277372866 1101 98-99 0.7109742866649071 1101 100-101 0.6244901393918489 1102 1 0.21734104594685988 1102 2 0.19240568379088785 1102 3 0.18775915820197753 1102 4 0.12405280653625539 1102 5 0.16311550032043698 1102 6 0.18560731932612384 1102 7 0.14245379715467976 1102 8 0.1830904511633733 1102 9 0.2662780439768895 1102 10-11 0.2212295303737264 1102 12-13 0.24408455455613876 1102 14-15 0.3037237636748529 1102 16-17 0.2762127870053348 1102 18-19 0.22237243601531986 1102 20-21 0.3126943283758621 1102 22-23 0.3588059197937028 1102 24-25 0.2956899273196356 1102 26-27 0.27650475767993754 1102 28-29 0.18585768229452526 1102 30-31 0.35465053890556675 1102 32-33 0.3910166555630994 1102 34-35 0.3816365754067377 1102 36-37 0.2887542453729992 1102 38-39 0.33076903088781506 1102 40-41 0.3180749634627773 1102 42-43 0.29185132127764035 1102 44-45 0.46548141737937954 1102 46-47 0.4726767462430246 1102 48-49 0.573888647627804 1102 50-51 0.468630650397877 1102 52-53 0.4424710402933627 1102 54-55 0.36580560691017183 1102 56-57 0.42072737342844135 1102 58-59 0.45658532578084987 1102 60-61 0.4459958575184686 1102 62-63 0.45076806260816227 1102 64-65 0.3613697372408211 1102 66-67 0.4861274194926395 1102 68-69 0.4435033929851713 1102 70-71 0.3640012477993402 1102 72-73 0.49052256026072527 1102 74-75 0.566162195300457 1102 76-77 0.2595095469512785 1102 78-79 0.30740813198130823 1102 80-81 0.44617663760887893 1102 82-83 0.6355381029758185 1102 84-85 0.49963891671841054 1102 86-87 0.2800127617089352 1102 88-89 0.4792568646062598 1102 90-91 0.5804580849239436 1102 92-93 0.45012487957973235 1102 94-95 0.41523581084481975 1102 96-97 0.29059688832505515 1102 98-99 0.14970776958686827 1102 100-101 -0.15681876113171356 1103 1 0.1332847984600818 1103 2 0.1622616833441981 1103 3 0.21669388631495679 1103 4 0.1912821341934432 1103 5 0.1655651154741875 1103 6 0.11475265130076195 1103 7 0.20227551560473955 1103 8 0.09851298501106953 1103 9 0.15335393746725856 1103 10-11 0.24740337559857295 1103 12-13 0.25564750358658017 1103 14-15 0.34639783832597715 1103 16-17 0.3056901228867446 1103 18-19 0.2294651751128498 1103 20-21 0.26361984611598643 1103 22-23 0.33277819591396707 1103 24-25 0.32467959165480664 1103 26-27 0.2793446676745859 1103 28-29 0.40986677018662476 1103 30-31 0.39929252359817013 1103 32-33 0.39350353278559425 1103 34-35 0.43535857200519956 1103 36-37 0.5944197234059416 1103 38-39 0.6554513351651394 1103 40-41 0.6183641216192086 1103 42-43 0.6554909495665555 1103 44-45 0.6321361887028587 1103 46-47 0.6503688575365985 1103 48-49 0.5873196533614262 1103 50-51 0.5220014154118005 1103 52-53 0.4108293523464184 1103 54-55 0.5007491194151399 1103 56-57 0.6604861583832999 1103 58-59 0.5027998598338783 1103 60-61 0.5431913183455706 1103 62-63 0.5702314933212023 1103 64-65 0.5840554025342328 1103 66-67 0.5664373093184949 1103 68-69 0.5385681614402458 1103 70-71 0.4827740356370107 1103 72-73 0.55619205944501 1103 74-75 0.6516176278399008 1103 76-77 0.7597090284794241 1103 78-79 0.7370437247093733 1103 80-81 0.7910242503823639 1103 82-83 0.8229647327241452 1103 84-85 0.6778453796497423 1103 86-87 0.7931493858420531 1103 88-89 0.979170182452382 1103 90-91 0.9597599401021562 1103 92-93 1.046903703197085 1103 94-95 0.7371090504653637 1103 96-97 0.8188138431742331 1103 98-99 0.4800881308780873 1103 100-101 0.4208855982376356 1104 1 0.19816991242375792 1104 2 0.13411005854810298 1104 3 0.14965133759039873 1104 4 0.13903417677291685 1104 5 0.12945575391148623 1104 6 0.12551522894337808 1104 7 0.10469024378640768 1104 8 0.14065675133347355 1104 9 0.21620152295869133 1104 10-11 0.13625595586771766 1104 12-13 0.11616640354081653 1104 14-15 0.28759236849096936 1104 16-17 0.23822263154591639 1104 18-19 0.22807505275572737 1104 20-21 0.27891448617312165 1104 22-23 0.2713765133608135 1104 24-25 0.2239829053727007 1104 26-27 0.24987055996624008 1104 28-29 0.2613496552109922 1104 30-31 0.2536676537162563 1104 32-33 0.23765742042856886 1104 34-35 0.39838966243210905 1104 36-37 0.4133300431648266 1104 38-39 0.5546657074052064 1104 40-41 0.6452479460412661 1104 42-43 0.7255671713150065 1104 44-45 0.7364739853611155 1104 46-47 0.755423676232148 1104 48-49 0.8424172078880474 1104 50-51 0.7606729064399076 1104 52-53 0.78577873256706 1104 54-55 0.875299251224213 1104 56-57 0.8759467786106612 1104 58-59 0.8796075679195994 1104 60-61 1.0372919728669956 1104 62-63 1.073917184240564 1104 64-65 1.0373088598322227 1104 66-67 0.9811663694201016 1104 68-69 1.1445692735381314 1104 70-71 0.9469253494673389 1104 72-73 1.1597155474618468 1104 74-75 1.0135444384468215 1104 76-77 1.0454659182596338 1104 78-79 1.2720260550024456 1104 80-81 1.2405990442357826 1104 82-83 1.2649272341186588 1104 84-85 1.1604170225591517 1104 86-87 0.9867249123097217 1104 88-89 0.9986764759952713 1104 90-91 1.1228564299663404 1104 92-93 1.2527208711423157 1104 94-95 1.1546587632612528 1104 96-97 1.190960578604738 1104 98-99 1.1678306336605289 1104 100-101 1.061528208101226 1105 1 0.15817056873004276 1105 2 0.09945708680587018 1105 3 0.08709377931902651 1105 4 0.08804906524922984 1105 5 0.09016299124488114 1105 6 0.09005828200447752 1105 7 0.08867700154502245 1105 8 0.10751075978720337 1105 9 0.08923696896719235 1105 10-11 0.18177952630851735 1105 12-13 0.182430093942628 1105 14-15 0.14695255821396813 1105 16-17 0.17237880888155388 1105 18-19 0.23146481160102894 1105 20-21 0.27281052220938307 1105 22-23 0.2110279936890791 1105 24-25 0.27216756640103057 1105 26-27 0.2553266318300942 1105 28-29 0.2772575098088623 1105 30-31 0.21258698231493156 1105 32-33 0.03389314629280449 1105 34-35 0.1177817040452922 1105 36-37 0.10238022493221877 1105 38-39 0.16880567902417454 1105 40-41 0.24624044101063447 1105 42-43 0.18059073313126817 1105 44-45 0.052221781160668 1105 46-47 -0.008939123991162035 1105 48-49 -2.7490579261524317E-4 1105 50-51 0.015580373564638705 1105 52-53 0.07499008801202223 1105 54-55 -0.04429923237170641 1105 56-57 0.039479162066861306 1105 58-59 -0.0421098173393446 1105 60-61 -0.1163948697486532 1105 62-63 -0.11605277291862848 1105 64-65 0.08789703226744194 1105 66-67 0.08156366611134303 1105 68-69 0.1830556419304301 1105 70-71 0.058490160343847464 1105 72-73 0.1064642860706222 1105 74-75 0.13767225577827347 1105 76-77 0.1362200352142544 1105 78-79 0.20911815118160604 1105 80-81 0.1733500529911609 1105 82-83 0.24265562765336668 1105 84-85 0.32729278167623477 1105 86-87 0.2372016809604922 1105 88-89 0.40162192390117113 1105 90-91 0.48537519885354996 1105 92-93 0.6148659451219167 1105 94-95 0.8012503459648919 1105 96-97 0.8208491664061128 1105 98-99 0.8713087203930883 1105 100-101 0.37269584849923376 1106 1 0.1873172393388316 1106 2 0.15480685606748779 1106 3 0.18565246066289376 1106 4 0.16974996308374557 1106 5 0.13705742768355833 1106 6 0.1865367323335292 1106 7 0.22582321704693697 1106 8 0.14125276787416396 1106 9 0.09096785738307744 1106 10-11 0.2321413482904191 1106 12-13 0.2601185596675961 1106 14-15 0.3610009181265852 1106 16-17 0.30995519252062564 1106 18-19 0.302153505280053 1106 20-21 0.28711463936414816 1106 22-23 0.28920652057704643 1106 24-25 0.25897116975776413 1106 26-27 0.29932577129351046 1106 28-29 0.2628279978909447 1106 30-31 0.1322496022962909 1106 32-33 0.12610196503006676 1106 34-35 0.33074547938114307 1106 36-37 0.1856449517058465 1106 38-39 0.35899187622565876 1106 40-41 0.30400015339591135 1106 42-43 0.25018302387725555 1106 44-45 0.3488255661595332 1106 46-47 0.26536262147990186 1106 48-49 0.12115099291325038 1106 50-51 0.2019147881774117 1106 52-53 0.2802464922591028 1106 54-55 0.16579759001282213 1106 56-57 0.24385399706057598 1106 58-59 0.25139936111663985 1106 60-61 0.2950186377111308 1106 62-63 0.3610025894702389 1106 64-65 0.2845812221511679 1106 66-67 0.2539738536145748 1106 68-69 0.3454681009617886 1106 70-71 0.307594168689608 1106 72-73 0.3298708811941715 1106 74-75 0.3769864585980933 1106 76-77 0.5580844750622269 1106 78-79 0.49593062832110135 1106 80-81 0.4034562539424762 1106 82-83 0.45262995978603726 1106 84-85 0.4986453264487771 1106 86-87 0.648988568353996 1106 88-89 0.8117569456168994 1106 90-91 1.031553222559971 1106 92-93 0.9862589928035774 1106 94-95 1.1354236839935155 1106 96-97 0.9129274824465838 1106 98-99 1.2247699163226038 1106 100-101 1.3338800598162486 1107 1 0.13569599261224852 1107 2 0.0601590415265818 1107 3 0.11317213380671376 1107 4 0.010415254540355079 1107 5 0.09067000313870466 1107 6 0.03370087029527724 1107 7 0.08234987873029098 1107 8 0.06304815796428187 1107 9 0.10315451431347356 1107 10-11 0.08074269977407056 1107 12-13 0.01111762174515718 1107 14-15 0.14371590603494866 1107 16-17 0.21437531036176694 1107 18-19 0.21349766368950895 1107 20-21 0.20775835822083621 1107 22-23 0.2413648939438886 1107 24-25 0.14072143744765953 1107 26-27 0.13098289940803198 1107 28-29 0.1748069719965315 1107 30-31 0.2213587602661491 1107 32-33 0.2339628530502651 1107 34-35 0.24017549666149307 1107 36-37 0.16003310416757444 1107 38-39 0.15382374316737213 1107 40-41 0.24776127994709896 1107 42-43 0.14596479163261478 1107 44-45 0.18954547057821003 1107 46-47 0.11094144785214866 1107 48-49 0.15737224816291473 1107 50-51 0.3623248633762941 1107 52-53 0.2431528693254208 1107 54-55 0.2870507472946926 1107 56-57 0.3481035189811976 1107 58-59 0.27671626026751284 1107 60-61 0.07102285394698526 1107 62-63 0.08743695236828586 1107 64-65 0.10299485093582916 1107 66-67 0.20328803382698624 1107 68-69 0.326091921120252 1107 70-71 0.29592302356809697 1107 72-73 0.3823430919307107 1107 74-75 0.48263777719878576 1107 76-77 0.17946960567070747 1107 78-79 0.3602799009609079 1107 80-81 0.37570288723092915 1107 82-83 0.5561072217896594 1107 84-85 0.600265171858581 1107 86-87 0.5612915729691252 1107 88-89 0.8933650276442258 1107 90-91 0.9785512948855803 1107 92-93 0.9159810368218118 1107 94-95 1.0925258261653568 1107 96-97 0.9698089460202155 1107 98-99 0.9105603338497374 1107 100-101 0.7382157147283657 1108 1 0.0205882537511215 1108 2 0.017238593243298794 1108 3 -0.03416034840591209 1108 4 -0.007992286614197042 1108 5 0.03620841736467639 1108 6 0.03656436038852462 1108 7 0.07076014463348201 1108 8 0.02519529698749068 1108 9 0.1403234986239994 1108 10-11 -0.013579324708608453 1108 12-13 -0.04121174156696128 1108 14-15 0.012284159133493233 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-1.2405503493366155 2316 94-95 -1.0989857379741572 2316 96-97 -1.1586794137176284 2316 98-99 -0.9853250628776848 2316 100-101 -0.7119379102794454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 33.0 5 71.0 6 252.0 7 462.0 8 1117.0 9 1804.0 10 3120.0 11 4979.0 12 7470.0 13 10375.0 14 12497.0 15 17233.0 16 22958.0 17 33902.0 18 30894.0 19 17588.0 20 14672.0 21 15147.0 22 15761.0 23 17112.0 24 19502.0 25 22730.0 26 26350.0 27 31457.0 28 37847.0 29 45321.0 30 54789.0 31 66246.0 32 82121.0 33 105275.0 34 145566.0 35 215089.0 36 314815.0 37 487268.0 38 380173.0 39 27819.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03144314725792 20.236664517666185 14.725923603032895 21.005968732042998 2 15.488942458861754 22.632760367536598 39.148674143171576 22.729623030430073 3 19.781993501729378 27.176789644691336 30.468504349648885 22.572712503930408 4 12.193603046501066 17.279067533102747 38.29049191209866 32.23683750829752 5 12.32238933724627 39.57486112566817 33.343770743807426 14.758978793278132 6 29.150377318939313 37.765127694511406 17.741719945498374 15.342775041050905 7 26.90611571114139 35.32932431960312 20.781320965656988 16.983239003598506 8 26.344853788911017 33.338704887677736 20.278054361876812 20.038386961534428 9 27.293653705062365 17.27736435733501 21.47713377353876 33.95184816406386 10-11 24.577219369038886 27.468644097404187 26.91998130873773 21.0341552248192 12-13 26.633258218914857 25.460687209586695 27.66730426580023 20.238750305698215 14-15 22.731704419823753 26.25108169525967 28.25856398035032 22.758649904566255 16-17 20.305290438831534 29.80490383630675 28.485192828517707 21.404612896344013 18-19 20.050981772509672 27.678508261283113 32.47614023350123 19.79436973270598 20-21 21.491086713482165 26.16772350906614 32.16916902491004 20.172020752541663 22-23 20.648276735492434 26.57080844076442 32.229937462879505 20.55097736086364 24-25 20.79022988505747 26.769577787094295 32.50768612654159 19.93250620130664 26-27 20.435829863110435 26.56868595754679 33.16032986605824 19.83515431328454 28-29 19.731575131886945 27.665317227404536 32.852426370401425 19.750681270307098 30-31 20.160797260943998 26.89113649861999 32.45820668693009 20.48985955350592 32-33 19.791251790518114 27.289330258882714 32.429558397093246 20.48985955350592 34-35 20.89930782330639 26.95340281085009 32.27405122400134 19.87323814184218 36-37 20.841250253585297 26.50321761254438 32.44140459940948 20.21412753446085 38-39 20.156945277251648 27.311456695165777 32.389566853395024 20.14203117418755 40-41 20.293589080102105 27.33699746480534 32.28093275947532 20.088480695617235 42-43 20.03321203625156 27.67171834621091 31.39592088067577 20.899148736861758 44-45 20.336080359523358 27.328648955295186 31.296446276426053 21.038824408755403 46-47 20.9684804731521 28.096659387392407 30.45246283082904 20.482397308626453 48-49 20.90573952962894 28.043429415408696 30.228154441999088 20.822676612963274 50-51 20.299365894560317 28.364121510673236 30.572834783216297 20.763677811550153 52-53 20.967893375909835 28.85764020963919 29.538386759942508 20.63607965450846 54-55 20.13040212416588 28.163648988575623 30.016966250917093 21.688982636341404 56-57 20.818740674764385 28.503160617541585 29.379757330429467 21.29834137726456 58-59 21.275012866863932 27.884311990170307 29.57066272046446 21.2700124225013 60-61 20.563842215016432 28.45300405052788 30.336335746181476 20.646817988274215 62-63 20.47598625914594 28.28587824163884 30.26608274733232 20.972052751882906 64-65 20.039640341526194 28.67808610124991 29.96103656195916 21.321236995264734 66-67 20.3504675244025 28.448481042454297 29.350819952773726 21.850231480369477 68-69 20.351012422455188 28.316559707049286 29.634685049600296 21.69774282089523 70-71 20.61320878314642 28.47884568354121 29.4852915487545 21.422653984557876 72-73 20.70321507179541 28.05161059287985 29.777364357335014 21.46780997798973 74-75 19.914435032331966 29.31810926445047 29.08350761778448 21.68394808543308 76-77 20.157175405116245 28.526129748850803 29.296922804444975 22.019772041587974 78-79 20.217002298198018 28.601505565351253 29.384530561666494 21.796961574784238 80-81 20.463534772532043 28.777263379652457 29.031669912164134 21.72753193565137 82-83 20.353234287111764 28.596779687663766 29.075721447786744 21.974264577437726 84-85 19.87289504943577 28.66833490549558 29.057204870209276 22.40156517485938 86-87 20.035237321832383 28.722985903978298 28.795100001202268 22.44667677298705 88-89 19.945595449498366 28.615561779258066 28.520723132081937 22.91811963916163 90-91 19.873333517263365 28.28419116986946 28.851681011019597 22.99079430184758 92-93 20.306156762044512 28.47812510917793 28.808257345491388 22.40746078328617 94-95 19.911019983929005 28.004642245746425 29.04113387834958 23.043203891974986 96-97 19.536605177654334 27.967368899137057 29.306851133703667 23.189174789504943 98-99 19.61026475034353 28.297457045513948 28.3766330041468 23.71564519999572 100-101 19.520763236198693 27.59075625095941 29.325951703369924 23.562528809471974 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3743.0 1 2570.0 2 1924.5 3 2521.0 4 2145.5 5 1979.5 6 3674.0 7 7293.5 8 10862.5 9 11227.0 10 8667.5 11 6655.0 12 6308.5 13 6450.0 14 6548.5 15 6693.0 16 6788.0 17 6943.5 18 7172.0 19 7433.0 20 8000.5 21 8981.5 22 10059.5 23 11432.5 24 13084.0 25 15258.5 26 19095.0 27 23390.0 28 26096.0 29 29441.0 30 34599.0 31 39630.0 32 46513.5 33 52228.5 34 56482.0 35 60006.5 36 63972.5 37 69012.0 38 69007.5 39 66681.5 40 65270.5 41 66800.5 42 68262.0 43 69408.5 44 70403.5 45 70832.0 46 70590.5 47 78444.0 48 103590.0 49 102547.0 50 77694.0 51 65229.5 52 58313.5 53 56599.5 54 56377.5 55 51776.5 56 45711.0 57 41124.5 58 39264.5 59 36022.5 60 31034.0 61 27009.0 62 23423.5 63 18162.5 64 12745.5 65 10066.0 66 7346.0 67 5702.5 68 4520.0 69 3112.5 70 2396.5 71 2059.0 72 1860.5 73 1863.0 74 1643.0 75 1439.0 76 1068.5 77 545.5 78 301.5 79 198.5 80 133.0 81 91.0 82 67.0 83 41.0 84 19.5 85 9.0 86 5.5 87 6.0 88 4.0 89 1.0 90 1.5 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2885791146979702 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001375641966250917 16-17 8.952590574013905E-4 18-19 8.73423470635503E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.550676029766272E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015066554868462425 36-37 0.007707962128358313 38-39 0.002663941585438284 40-41 0.0032753380148831364 42-43 3.2753380148831357E-4 44-45 0.001331970792719142 46-47 2.401914544247633E-4 48-49 4.148761485518639E-4 50-51 0.0 52-53 4.367117353177515E-5 54-55 0.0 56-57 8.079167103378401E-4 58-59 0.001899696048632219 60-61 6.550676029766271E-4 62-63 0.0011354505118261538 64-65 4.367117353177515E-5 66-67 5.677252559130769E-4 68-69 0.0015284910736121302 70-71 0.0 72-73 0.0 74-75 4.367117353177515E-5 76-77 0.2602146874890822 78-79 1.0917793382943785E-4 80-81 4.367117353177515E-5 82-83 0.0 84-85 0.0 86-87 0.10885040002794955 88-89 0.3754410788526709 90-91 0.27787967718268525 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.52849107361213E-4 100-101 5.022184956154142E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2289840.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.59364415736436 #Duplication Level Percentage of deduplicated Percentage of total 1 69.50714171161344 26.13026751901893 2 14.647480991253575 11.013043763738908 3 5.959139425344241 6.720773011215362 4 2.912902263525588 4.3802644464064855 5 1.7478651242613175 3.2854309758273708 6 1.1124070695738248 2.5091661319016896 7 0.7294724332208316 1.9196468953977475 8 0.5352083080820749 1.6096344547282044 9 0.3884015696081879 1.3141287358210798 >10 2.0717974792972003 14.480593652934685 >50 0.21603802075319958 5.635532193975849 >100 0.1558198165477611 10.96773845410603 >500 0.010961599788355708 2.798286406623177 >1k 0.00454789778453056 3.551834761551751 >5k 3.498382911177354E-4 0.766612373182615 >10k+ 4.664510548236472E-4 2.9170462235701495 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23132 1.0102015861370228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15480 0.6760297662718793 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15213 0.6643695629388953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12715 0.5552789714565209 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6524 0.284910736121301 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5696 0.24875100443699122 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5267 0.2300160709918597 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4362 0.1904936589456032 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4343 0.18966390664849947 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4096 0.178877126786151 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3581 0.15638647241728681 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3578 0.15625545889669148 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3450 0.15066554868462426 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3245 0.14171295811061033 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3148 0.13747685427802817 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2798 0.12219194354190685 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2533 0.11061908255598643 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2510 0.10961464556475563 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2485 0.10852286622646125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2314 0.10105509555252767 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1835586765887574E-5 0.0 12-13 0.0 0.0 0.0 4.367117353177515E-5 0.0 14-15 0.0 0.0 0.0 4.367117353177515E-5 0.0 16-17 0.0 0.0 0.0 1.0917793382943786E-4 0.0 18-19 0.0 0.0 0.0 2.401914544247633E-4 0.0 20-21 0.0 0.0 0.0 3.05698214722426E-4 0.0 22-23 0.0 0.0 0.0 0.001026272577996716 0.0 24-25 0.0 0.0 0.0 0.0020088739824616567 0.0 26-27 0.0 0.0 0.0 0.0029259686266289347 0.0 28-29 0.0 0.0 0.0 0.007489606260699437 0.0 30-31 0.0 0.0 0.0 0.021508052964399258 0.0 32-33 0.0 0.0 0.0 0.045985745728959235 0.0 34-35 0.0 0.0 0.0 0.08461289871781434 0.0 36-37 0.0 0.0 0.0 0.14184397163120566 0.0 38-39 0.0 0.0 0.0 0.2330075463787863 0.0 40-41 0.0 0.0 0.0 0.34078800265520737 0.0 42-43 0.0 0.0 0.0 0.4496384026831569 0.0 44-45 0.0 0.0 0.0 0.5806519232784824 0.0 46-47 2.1835586765887574E-5 0.0 0.0 0.745226740732977 0.0 48-49 4.367117353177515E-5 0.0 0.0 0.9144525381686057 0.0 50-51 4.367117353177515E-5 0.0 0.0 1.1040072668832757 0.0 52-53 4.367117353177515E-5 0.0 0.0 1.2929724347552667 0.0 54-55 4.367117353177515E-5 0.0 0.0 1.503052615029871 0.0 56-57 4.367117353177515E-5 0.0 0.0 1.7552754777626385 0.0 58-59 4.367117353177515E-5 0.0 0.0 2.0269975194773435 0.0 60-61 4.367117353177515E-5 0.0 0.0 2.332171680117388 0.0 62-63 4.367117353177515E-5 0.0 0.0 2.6432632847709883 0.0 64-65 4.367117353177515E-5 0.0 0.0 2.9646612863780875 0.0 66-67 4.367117353177515E-5 0.0 0.0 3.3349928379275404 0.0 68-69 4.367117353177515E-5 0.0 0.0 3.756135799881214 0.0 70-71 4.367117353177515E-5 0.0 0.0 4.21730339237676 0.0 72-73 4.367117353177515E-5 0.0 0.0 4.680348845334172 0.0 74-75 4.367117353177515E-5 0.0 0.0 5.165360898578067 0.0 76-77 4.367117353177515E-5 0.0 0.0 5.68982985710792 0.0 78-79 4.367117353177515E-5 0.0 0.0 6.29661897774517 4.367117353177515E-5 80-81 4.367117353177515E-5 0.0 0.0 6.953979317332216 4.367117353177515E-5 82-83 4.367117353177515E-5 0.0 0.0 7.602627257799671 4.367117353177515E-5 84-85 4.367117353177515E-5 0.0 0.0 8.269660762324005 4.367117353177515E-5 86-87 4.367117353177515E-5 0.0 0.0 8.970670439856061 4.367117353177515E-5 88-89 4.367117353177515E-5 0.0 0.0 9.714084826887468 4.367117353177515E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 120 3.7343852E-9 35.685974 1 GTAAGCG 185 0.0 26.934841 94-95 GTACATG 15830 0.0 26.540928 1 TACATGG 15805 0.0 26.212992 2 ACATGGG 15755 0.0 25.00095 3 GAGTACG 260 2.3646862E-11 23.79065 1 CATGGGG 4750 0.0 23.37863 4 GAGTACT 16590 0.0 23.271893 12-13 ATCGATC 105 0.0022085768 22.598392 8 CATGGGA 9875 0.0 22.538893 4 AGTACTT 16820 0.0 22.248272 12-13 GTACTTT 17905 0.0 21.562967 14-15 ATGGGAG 3455 0.0 21.427593 5 TAGTCGA 180 5.2135365E-6 21.091833 5 GTATCAA 34375 0.0 20.928852 1 CGGTATA 205 6.895607E-7 20.834616 2 ATGGGAT 3605 0.0 20.14109 5 GTACCGT 120 0.0047901645 19.773594 6 GCACCGT 480 0.0 19.773594 6 AGAGTAC 27520 0.0 19.418056 10-11 >>END_MODULE