FastQCFastQC Report
Fri 27 May 2016
SRR939232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336298
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240101.0276942410600018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148550.6358349833796888No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT141060.6037757169676129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122430.5240341771469221No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70660.3024442943494366No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63760.27291039071214374No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55780.23875378911423112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.19732071850423188No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41540.17780266044828186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41030.17561971974465587No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35950.15387591822618518No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.14882519267661917No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32940.14099228779890235No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30260.12952114841514223No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30120.12892190979061746No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.12322914285763202No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28730.12297232630426426No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24430.10456713997957454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.1024698047937378No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.1021701854814754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC905.699094E-736.946281
GTACATG169700.029.531521
TACATGG170100.029.0243872
ACATGGG170750.027.8584463
ATGGGCG4050.026.9332945
CATGGGA109900.025.7628154
CATGGGG49600.024.1910924
ATGGGAG39150.023.985575
CGTACAC1801.9824438E-723.7130073
GAGTACT154050.023.1223912-13
AGTACTT157550.022.1574512-13
ATGGGAT41350.021.6771875
GTATCAA340000.021.6135751
GTCGGGA9450.021.5800912
GTACTTT169050.021.08586114-15
CGGGAGT10200.020.4582824
CATGGGC23650.019.852754
AGTACAT124950.019.3575572
AGAGTAC264750.019.2542210-11
CAACGCA384900.018.938375
Produced by FastQC (version 0.11.5)