FastQCFastQC Report
Fri 27 May 2016
SRR939232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336298
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231520.9909694739284115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156910.6716180898155972No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT151510.6485046000124983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128850.5515135483572728No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63660.27248236312319746No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58220.2491976622845202No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53610.22946559043409703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45370.19419611710492413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.19372528675708323No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40790.1745924535311848No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.15558802858197027No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35650.15259183545934638No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34620.14818315129319976No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32690.1399222188265367No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG32490.13906616364864413No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28600.12241589043863411No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25630.10970347104692979No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.10863340207456411No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25140.10760613586109305No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.10448153446178526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.10234139651705391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAG856.3425966E-427.9911161
GTACATG168300.026.831891
TACATGG167650.026.607382
ACATGGG167200.025.5720943
ATTGCGT950.001233800924.9760746
TATATCG1201.7226994E-423.7272725
GTAAGCG1202.3283064E-1023.72727294-95
CATGGGA103900.023.1106834
CATGGGG50150.022.89934
CGTTAGA1050.00220915322.5974032
AGTACTT165900.022.34187512-13
GAGTACT164700.021.40968112-13
GTCGGGA8650.021.3956892
ATGGGAT39150.020.8484825
CATGGGC24100.020.0844944
GTACTTT175500.019.9434314-15
GTATCAA361650.019.697111
CGGGAGT10000.019.4563644
ATGGGAG37350.019.1851025
AGAGTAC281950.019.12126210-11
Produced by FastQC (version 0.11.5)