##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939232_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2336298 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23035503176393 33.0 31.0 34.0 31.0 34.0 2 32.41846673669198 34.0 31.0 34.0 31.0 34.0 3 32.502882337783966 34.0 31.0 34.0 30.0 34.0 4 36.05048114581273 37.0 35.0 37.0 35.0 37.0 5 35.99968197550141 37.0 35.0 37.0 35.0 37.0 6 36.055756585846495 37.0 35.0 37.0 35.0 37.0 7 35.99848392627995 37.0 35.0 37.0 35.0 37.0 8 35.98542138032049 37.0 35.0 37.0 35.0 37.0 9 37.65950362496565 39.0 37.0 39.0 35.0 39.0 10-11 37.584640529589976 39.0 37.0 39.0 35.0 39.0 12-13 37.51019219294798 39.0 37.0 39.0 35.0 39.0 14-15 38.727753480078306 40.0 38.0 41.0 34.0 41.0 16-17 38.70627398559601 40.0 38.0 41.0 34.0 41.0 18-19 38.86298537258517 40.0 38.0 41.0 35.0 41.0 20-21 38.875507533713595 40.0 38.0 41.0 35.0 41.0 22-23 38.80659209570012 40.0 38.0 41.0 34.5 41.0 24-25 38.7197540724685 40.0 38.0 41.0 34.0 41.0 26-27 38.520993041127454 40.0 38.0 41.0 34.0 41.0 28-29 38.357341828824914 40.0 38.0 41.0 34.0 41.0 30-31 38.09185151038095 40.0 37.0 41.0 33.5 41.0 32-33 37.80143992761198 40.0 36.5 41.0 33.0 41.0 34-35 37.64712078681744 40.0 36.0 41.0 33.0 41.0 36-37 37.497566235129256 40.0 36.0 41.0 33.0 41.0 38-39 37.34177746160807 40.0 36.0 41.0 33.0 41.0 40-41 37.12716956484147 40.0 36.0 41.0 32.0 41.0 42-43 36.86411194119928 40.0 35.0 41.0 31.0 41.0 44-45 36.51351711125892 40.0 35.0 41.0 30.5 41.0 46-47 36.04972696120101 39.5 35.0 41.0 29.5 41.0 48-49 35.59771762848746 39.0 35.0 41.0 27.5 41.0 50-51 35.305991358979036 39.0 35.0 41.0 26.5 41.0 52-53 35.08141726783141 39.0 35.0 40.5 26.0 41.0 54-55 34.80410225921522 38.5 35.0 40.0 25.0 41.0 56-57 34.55438004912044 38.0 34.0 40.0 24.0 41.0 58-59 34.26384819059897 38.0 34.0 40.0 23.0 41.0 60-61 33.91409892916057 37.5 34.0 40.0 22.5 41.0 62-63 33.56508972742347 37.0 33.0 40.0 22.0 41.0 64-65 33.16191962669146 36.5 33.0 39.5 20.5 41.0 66-67 32.77773533170854 36.0 33.0 39.0 19.5 40.5 68-69 32.39757385402034 35.5 32.0 39.0 18.0 40.0 70-71 32.016138994255016 35.0 32.0 38.0 14.0 40.0 72-73 31.540589856259775 35.0 31.5 37.5 10.0 40.0 74-75 31.06228357855034 35.0 31.0 37.0 7.0 39.0 76-77 29.10385982438884 33.0 28.5 35.0 3.5 37.5 78-79 30.06047559001463 34.0 30.0 36.0 2.0 38.0 80-81 30.08815827432973 34.0 30.5 35.5 2.0 37.0 82-83 29.771549905020677 34.0 30.0 35.0 2.0 37.0 84-85 29.39203218082625 34.0 30.0 35.0 2.0 36.5 86-87 28.8871899047125 34.0 29.0 35.0 2.0 36.0 88-89 28.374041967249042 34.0 29.0 35.0 2.0 36.0 90-91 27.9391824159418 34.0 28.0 35.0 2.0 35.0 92-93 27.603556566842073 34.0 27.0 35.0 2.0 35.0 94-95 27.20846548685142 33.0 26.5 35.0 2.0 35.0 96-97 26.670606232595325 33.0 25.5 35.0 2.0 35.0 98-99 25.97789879544476 33.0 24.5 35.0 2.0 35.0 100-101 22.812523488013944 29.0 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1844634184167191 1101 2 0.22235181918422597 1101 3 0.1771555277720438 1101 4 0.1461663028316309 1101 5 0.20316868045177472 1101 6 0.20189755929701647 1101 7 0.20113494527870301 1101 8 0.1878063804691692 1101 9 0.2622791311023178 1101 10-11 0.26110634192148297 1101 12-13 0.25608118800305846 1101 14-15 0.2899089659346643 1101 16-17 0.3181166552483887 1101 18-19 0.3031852829590136 1101 20-21 0.34063979255739696 1101 22-23 0.3443590781562449 1101 24-25 0.35515848127972305 1101 26-27 0.3084229127116416 1101 28-29 0.3455788844843326 1101 30-31 0.3601011661559568 1101 32-33 0.2806506922782148 1101 34-35 0.44178622523910605 1101 36-37 0.41531562796799193 1101 38-39 0.4211624567346419 1101 40-41 0.3913225864405163 1101 42-43 0.3178854824300288 1101 44-45 0.32422044782164505 1101 46-47 0.37470643613324484 1101 48-49 0.37641391570934246 1101 50-51 0.39993084274437507 1101 52-53 0.369187971628385 1101 54-55 0.41888073241165813 1101 56-57 0.341411912199284 1101 58-59 0.33524392206389564 1101 60-61 0.380914637955307 1101 62-63 0.5116526172713804 1101 64-65 0.4194794625501075 1101 66-67 0.4147061606620852 1101 68-69 0.5043183638439501 1101 70-71 0.4674363100471268 1101 72-73 0.3577589186451746 1101 74-75 0.2958558647574314 1101 76-77 0.4574113074714674 1101 78-79 0.5715130000895918 1101 80-81 0.6589250865758878 1101 82-83 0.7191357953002289 1101 84-85 0.5015954875102899 1101 86-87 0.4940326805402293 1101 88-89 0.7057425957751562 1101 90-91 0.8197654117097883 1101 92-93 0.7685725684699669 1101 94-95 0.7420220485717444 1101 96-97 0.5023730516927642 1101 98-99 0.5646763267177484 1101 100-101 0.6090225954008872 1102 1 0.2168350631440532 1102 2 0.2082727946355334 1102 3 0.21036385142987513 1102 4 0.13329397511478192 1102 5 0.13488606015233273 1102 6 0.16461501192733863 1102 7 0.19615111286862685 1102 8 0.15089004791479965 1102 9 0.31249507927654463 1102 10-11 0.21696571185302815 1102 12-13 0.2603084300005847 1102 14-15 0.2771360777324148 1102 16-17 0.38728684871809094 1102 18-19 0.3623740533855013 1102 20-21 0.2370391425428764 1102 22-23 0.2416847033052889 1102 24-25 0.30288226716687916 1102 26-27 0.4096336587115772 1102 28-29 0.4528479465583004 1102 30-31 0.4732721703541145 1102 32-33 0.4717006214289583 1102 34-35 0.5798126338686203 1102 36-37 0.5643217697282523 1102 38-39 0.5471469526713832 1102 40-41 0.636524993391312 1102 42-43 0.5949000330372485 1102 44-45 0.5449402646292327 1102 46-47 0.7162499611107904 1102 48-49 0.679524236076837 1102 50-51 0.6254136892603199 1102 52-53 0.6755663733210682 1102 54-55 0.690167052442689 1102 56-57 0.5911156070539789 1102 58-59 0.5045700644326416 1102 60-61 0.5423610097441056 1102 62-63 0.4638077813924397 1102 64-65 0.6438305554004415 1102 66-67 0.6418998366221444 1102 68-69 0.7230635960044509 1102 70-71 0.6044613884913659 1102 72-73 0.7660801604019802 1102 74-75 0.8119926944243652 1102 76-77 0.7942272609252718 1102 78-79 0.927036893089543 1102 80-81 1.1086299447274932 1102 82-83 1.0272373305389522 1102 84-85 0.9605530303050749 1102 86-87 1.0018143538712465 1102 88-89 1.123394932861018 1102 90-91 1.22460425705707 1102 92-93 1.1173072817418905 1102 94-95 1.0690673146915763 1102 96-97 0.6577019358628462 1102 98-99 0.7635056920900674 1102 100-101 0.5191764772108165 1103 1 0.15942240931358498 1103 2 0.22006437509752175 1103 3 0.14936481204466645 1103 4 0.127859523864295 1103 5 0.0744696221228267 1103 6 0.09674805097924377 1103 7 0.11424820135222546 1103 8 0.1096378072706301 1103 9 0.2287251336481546 1103 10-11 0.1655545294297056 1103 12-13 0.2011357781211487 1103 14-15 0.20163466797261975 1103 16-17 0.13496050795590975 1103 18-19 0.0835159791404152 1103 20-21 0.15369226034081152 1103 22-23 0.13653396536615503 1103 24-25 0.17903984863960432 1103 26-27 0.27087935525214846 1103 28-29 0.20416760637331066 1103 30-31 0.2551316577462117 1103 32-33 0.27606003768949705 1103 34-35 0.41831452190348273 1103 36-37 0.35717751898746286 1103 38-39 0.21786777958593717 1103 40-41 0.30563113387410823 1103 42-43 0.3575577249332582 1103 44-45 0.5417769425008174 1103 46-47 0.5559973289043256 1103 48-49 0.49349115515892805 1103 50-51 0.5286358134790632 1103 52-53 0.47998032377827116 1103 54-55 0.5310203466468906 1103 56-57 0.7138046781299892 1103 58-59 0.6219296879908711 1103 60-61 0.5615238522425727 1103 62-63 0.33806208552930883 1103 64-65 0.5928871476702753 1103 66-67 0.4806737181969112 1103 68-69 0.3977837102485182 1103 70-71 0.47075065141935823 1103 72-73 0.5487827261512557 1103 74-75 0.6322591600258214 1103 76-77 0.6488692388433286 1103 78-79 0.6160612255029072 1103 80-81 0.6367248789409601 1103 82-83 0.5652849250050487 1103 84-85 0.5237537608600782 1103 86-87 0.5191965362959223 1103 88-89 0.6983543878713192 1103 90-91 0.6803309574062233 1103 92-93 0.5724408803620697 1103 94-95 0.6596428570866628 1103 96-97 0.5358647499086828 1103 98-99 0.39556312845441965 1103 100-101 0.314087928579184 1104 1 0.19015176734734496 1104 2 0.1132405454246097 1104 3 0.1825114040147895 1104 4 0.14523977168509106 1104 5 0.1579139072181519 1104 6 0.11101835712709374 1104 7 0.09985015252291163 1104 8 0.1691013670245738 1104 9 0.24998112076326606 1104 10-11 0.27689314103795937 1104 12-13 0.24156567833699683 1104 14-15 0.27505609107877405 1104 16-17 0.20781400290348984 1104 18-19 0.20068334444102476 1104 20-21 0.1710392555563871 1104 22-23 0.26983246577243136 1104 24-25 0.16583724742987727 1104 26-27 0.18390667243195935 1104 28-29 0.2931417009476647 1104 30-31 0.29704442661493147 1104 32-33 0.2720414109628351 1104 34-35 0.18438376107070553 1104 36-37 0.313406225796939 1104 38-39 0.31683148354425583 1104 40-41 0.3235394725739553 1104 42-43 0.43977890677694376 1104 44-45 0.48356249546201013 1104 46-47 0.4941165352140189 1104 48-49 0.42450253479305644 1104 50-51 0.34539716776219365 1104 52-53 0.40953056418472755 1104 54-55 0.46825292656178874 1104 56-57 0.5508632909498203 1104 58-59 0.5122724729657051 1104 60-61 0.6186195631174272 1104 62-63 0.5791447903554854 1104 64-65 0.5224601052030735 1104 66-67 0.5319228967587861 1104 68-69 0.5242647681159553 1104 70-71 0.5167534907309275 1104 72-73 0.4937075225860532 1104 74-75 0.475364479257415 1104 76-77 0.4917519960835044 1104 78-79 0.48791753737415533 1104 80-81 0.44811511439574403 1104 82-83 0.46105446930608096 1104 84-85 0.4468138170405176 1104 86-87 0.44577445472234345 1104 88-89 0.6390742112966947 1104 90-91 0.8340538005632361 1104 92-93 0.8300020618633503 1104 94-95 0.8648371115632543 1104 96-97 0.7421882760281129 1104 98-99 0.7248416193524214 1104 100-101 0.6339611999375663 1105 1 0.056238996462816715 1105 2 0.10262510791385893 1105 3 0.0955140298545345 1105 4 0.054554339870975355 1105 5 0.07379728824805909 1105 6 0.09339253423475924 1105 7 0.07276759868308602 1105 8 0.09579948309410469 1105 9 0.09157708526218045 1105 10-11 0.03770766540366566 1105 12-13 0.0672601859836206 1105 14-15 0.09335889354753846 1105 16-17 -0.020645852759599848 1105 18-19 0.03830094262350059 1105 20-21 0.04899901346676927 1105 22-23 0.08559411941102013 1105 24-25 0.09002307699635281 1105 26-27 0.1174708622474796 1105 28-29 0.20082354624896226 1105 30-31 0.1867852808386914 1105 32-33 0.14810868504454078 1105 34-35 0.14558310241975647 1105 36-37 0.15651651443706527 1105 38-39 0.1995838624741637 1105 40-41 0.2556013466617415 1105 42-43 0.19598978462988725 1105 44-45 0.36687063219443417 1105 46-47 0.3169831668245422 1105 48-49 0.2965040128812859 1105 50-51 0.42140810763754644 1105 52-53 0.36115056236450727 1105 54-55 0.26192120614799563 1105 56-57 0.21343403525236226 1105 58-59 0.30271091067532296 1105 60-61 0.12641892233163077 1105 62-63 0.09859231063916951 1105 64-65 0.16200355796323862 1105 66-67 0.12572609192373818 1105 68-69 0.2285026004329893 1105 70-71 0.351057188142498 1105 72-73 0.23799973405547803 1105 74-75 0.1888148714479847 1105 76-77 0.434984854810633 1105 78-79 0.4957225847842146 1105 80-81 0.4615236667456877 1105 82-83 0.40026429884559533 1105 84-85 0.24673279185065766 1105 86-87 0.27227900359363844 1105 88-89 0.6046102753011162 1105 90-91 0.5541250420562207 1105 92-93 0.5823220030769001 1105 94-95 0.4669931063317989 1105 96-97 0.4067764337942279 1105 98-99 0.46272597285314987 1105 100-101 0.4209020292175296 1106 1 0.16501608226322162 1106 2 0.17252720829692691 1106 3 0.14449540080075707 1106 4 0.0435791871325506 1106 5 0.09566988610372817 1106 6 0.10232111993207837 1106 7 0.2071222778405044 1106 8 0.09576632251540218 1106 9 0.25150815920147807 1106 10-11 0.22916156500780716 1106 12-13 0.18258219118383323 1106 14-15 0.18581548115618318 1106 16-17 0.27221559355302816 1106 18-19 0.12541857717953775 1106 20-21 0.30667319040522045 1106 22-23 0.2541184776539467 1106 24-25 0.3059704587489307 1106 26-27 0.3073112799589026 1106 28-29 0.29869000260564604 1106 30-31 0.3742723961560017 1106 32-33 0.3140637976067282 1106 34-35 0.38958443363720363 1106 36-37 0.43611892309365885 1106 38-39 0.49548010102576967 1106 40-41 0.5261557116326614 1106 42-43 0.4829833067308158 1106 44-45 0.5291523117338031 1106 46-47 0.49932260170436393 1106 48-49 0.5306279863016883 1106 50-51 0.5800412245881077 1106 52-53 0.5353810371760517 1106 54-55 0.4759336881094356 1106 56-57 0.5627289934061039 1106 58-59 0.5831095727458546 1106 60-61 0.5408205816390463 1106 62-63 0.5586962390890307 1106 64-65 0.5199249609993117 1106 66-67 0.6021108657046668 1106 68-69 0.5393584549930921 1106 70-71 0.5214080750081891 1106 72-73 0.6581915100721538 1106 74-75 0.7719661212422331 1106 76-77 0.704204706251236 1106 78-79 0.7149291919695919 1106 80-81 0.6430468719593243 1106 82-83 0.7192457936445784 1106 84-85 0.7632902083938724 1106 86-87 0.7524515744414124 1106 88-89 0.9062306090483574 1106 90-91 0.9286771744811979 1106 92-93 0.8532842831183878 1106 94-95 1.0315019182561826 1106 96-97 0.9448138149555021 1106 98-99 0.9981164007125116 1106 100-101 1.0606940444697983 1107 1 0.0015221049560736333 1107 2 0.08228816447222442 1107 3 0.045417443623612996 1107 4 0.025109789867805432 1107 5 -0.0066162908259812525 1107 6 0.09946004959355292 1107 7 0.04504893378980768 1107 8 0.11068326294083874 1107 9 0.03030816386120705 1107 10-11 0.14804160042171333 1107 12-13 0.106104476312332 1107 14-15 0.17437178226752792 1107 16-17 0.1401939724351564 1107 18-19 0.21062977915526204 1107 20-21 0.15913265080921946 1107 22-23 0.22331420934705193 1107 24-25 0.14458558887171336 1107 26-27 0.23166270466941086 1107 28-29 0.24782620632876728 1107 30-31 0.21900312237393393 1107 32-33 0.22522645691103804 1107 34-35 0.10905450656188265 1107 36-37 0.17483860576646748 1107 38-39 0.11172681009592367 1107 40-41 0.20131909319412955 1107 42-43 0.1708916150181352 1107 44-45 0.1868679404470157 1107 46-47 0.2240252530871345 1107 48-49 0.30551265814064266 1107 50-51 0.2386922928297608 1107 52-53 0.24124588189075524 1107 54-55 0.24787661814368533 1107 56-57 0.22888862389001474 1107 58-59 0.295335522747024 1107 60-61 0.20623873359263456 1107 62-63 0.3032688259346301 1107 64-65 0.20586162370521066 1107 66-67 0.2854599256054158 1107 68-69 0.3252875456480169 1107 70-71 0.2921490875661519 1107 72-73 0.3281226044082537 1107 74-75 0.43361979892913993 1107 76-77 0.4000269727408359 1107 78-79 0.5842478238747191 1107 80-81 0.5186013790770581 1107 82-83 0.5213074301395402 1107 84-85 0.4005455729007572 1107 86-87 0.3226811823256668 1107 88-89 0.505952190558574 1107 90-91 0.6124354163674504 1107 92-93 0.4574407566395067 1107 94-95 0.5085315191508499 1107 96-97 0.6679432271313601 1107 98-99 0.7956982953561571 1107 100-101 0.8819033592574321 1108 1 0.009631928169675064 1108 2 0.03851949183031422 1108 3 0.06058973735409978 1108 4 0.10357026564110328 1108 5 0.003544434290269294 1108 6 0.05917431605355716 1108 7 0.11360588955950846 1108 8 0.09834527969847784 1108 9 0.049910225994771906 1108 10-11 0.052544374642799596 1108 12-13 -5.064812993680334E-4 1108 14-15 0.07938503563141097 1108 16-17 0.10681346611173126 1108 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2316 68-69 -0.5935347606694492 2316 70-71 -0.6825153917638431 2316 72-73 -0.8268304388287468 2316 74-75 -0.6885694895734673 2316 76-77 -0.8722345817522381 2316 78-79 -0.8370440274896218 2316 80-81 -1.0083468991212001 2316 82-83 -0.7875626556118043 2316 84-85 -0.9325113591498564 2316 86-87 -1.0101132209314656 2316 88-89 -1.3996855245456636 2316 90-91 -1.8222704062066377 2316 92-93 -1.6136186498760487 2316 94-95 -1.3903768288615161 2316 96-97 -1.2771317972261471 2316 98-99 -1.3366374285619465 2316 100-101 -0.7185520132672991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 30.0 5 70.0 6 235.0 7 567.0 8 1029.0 9 1856.0 10 3092.0 11 5045.0 12 7448.0 13 10991.0 14 12835.0 15 17394.0 16 23592.0 17 33567.0 18 30417.0 19 17297.0 20 14761.0 21 14806.0 22 15361.0 23 16634.0 24 18611.0 25 21746.0 26 26027.0 27 30856.0 28 37205.0 29 44798.0 30 54421.0 31 65801.0 32 80306.0 33 102852.0 34 141168.0 35 207037.0 36 302155.0 37 486046.0 38 444095.0 39 46117.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9693046461081 20.326508328383117 14.700128419512899 21.004058605995887 2 15.401545522018168 22.677629309274757 39.201463169510056 22.719361999197023 3 19.803424049500535 27.260049873774665 30.394281893833746 22.542244182891054 4 12.226565275491396 17.321463272236677 38.195598335486316 32.25637311678561 5 12.298602318711055 39.61018671419485 33.35524834588738 14.735962621206713 6 29.22773550291958 37.755543171290654 17.723466783775017 15.293254542014761 7 26.943523471748893 35.26703357191591 20.812327879405796 16.9771150769294 8 26.305377139388895 33.35058284516787 20.330754039082343 20.01328597636089 9 27.222982684571917 17.34804378551024 21.501923127957134 33.927050401960706 10-11 24.599366176746287 27.44992291223123 26.90497958736428 21.0457313236582 12-13 26.5909785481133 25.492980775568867 27.69578195932197 20.22025871699586 14-15 22.728965426024843 26.2757160339469 28.222714561921585 22.772603978106673 16-17 20.312431782752626 29.802344404751818 28.486880458984903 21.398343353510658 18-19 20.081534975418375 27.63611491342286 32.53782265789724 19.744527453261526 20-21 21.481998443691687 26.232227224437977 32.150329281624174 20.135445050246158 22-23 20.620122090589472 26.61854352484144 32.203426104032964 20.55790828053613 24-25 20.786590917165068 26.79986748260174 32.50928713258631 19.90425446764688 26-27 20.469149642196673 26.586080929447565 33.070842212338945 19.873927216016817 28-29 19.688284628073987 27.669137241910065 32.88623711529951 19.756341014716444 30-31 20.161383522136305 26.930789650977744 32.434346988269475 20.47347983861648 32-33 19.81750615717687 27.30319505474045 32.42831179926534 20.450986988817352 34-35 20.882963154472645 26.952187150743534 32.274625644151456 19.89022405063237 36-37 20.789333531979967 26.5504070115412 32.4286135064299 20.231645950048936 38-39 20.158399024367483 27.35079943265034 32.35083046694195 20.139971076040222 40-41 20.26406852090719 27.399950596586887 32.26049298099082 20.07548790151511 42-43 19.987419523367304 27.710201294046914 31.40988447064509 20.89249471194069 44-45 20.356459765854314 27.368460251241235 31.292389762273835 20.982690220630616 46-47 20.95094563166346 28.126365008193527 30.444115481111016 20.478573879031998 48-49 20.912229518665857 28.08496604457137 30.197667420851275 20.805137015911498 50-51 20.260621718633494 28.37578938988091 30.552844714158894 20.810744177326693 52-53 20.989766716403473 28.910288841577575 29.500196464663325 20.59974797735563 54-55 20.153957243468085 28.20571262741311 29.974472434595246 21.665857694523556 56-57 20.806506704196124 28.509226990734916 29.444638483618103 21.23962782145086 58-59 21.260595616672543 27.923996413964137 29.545823721383247 21.269584247980074 60-61 20.61964135939032 28.459851166736616 30.314220915373152 20.606286558499914 62-63 20.48020268261692 28.37021594524761 30.170933201670103 20.978648170465362 64-65 20.07830336712183 28.691630947764367 29.952493217902855 21.27757246721095 66-67 20.350507512312213 28.541735686115384 29.272892413553407 21.834864388018993 68-69 20.344589144453202 28.383639955579138 29.625673530613394 21.64609736935427 70-71 20.59127303109449 28.536599355048033 29.47881648659546 21.39331112726202 72-73 20.748765782447272 28.042933735336845 29.780490331284792 21.42781015093109 74-75 19.950622737339156 29.34816106506961 29.04802811970048 21.653188077890746 76-77 20.136915364998952 28.5157041018407 29.356468181646573 21.990912351513778 78-79 20.188691682311074 28.691652349143816 29.34841788162298 21.771238086922132 80-81 20.405806021922178 28.86184951700664 29.047230963623978 21.685113497447205 82-83 20.350486110932767 28.6284540756359 29.070007336392873 21.95105247703846 84-85 19.88656840865335 28.70423636025884 29.081778095088893 22.327417135998918 86-87 20.089181943976993 28.723514335888233 28.84327196700945 22.34403175312532 88-89 19.947010320659295 28.62426361162723 28.58257547625097 22.846150591462504 90-91 19.906604864304928 28.3153458276855 28.85674293592433 22.92130637208524 92-93 20.299529426468713 28.52279546530451 28.85278333500264 22.324891773224135 94-95 19.906266198176002 28.064304324321782 29.06109540011394 22.968334077388278 96-97 19.550267132018263 28.038460847032354 29.316101798657535 23.09517022229185 98-99 19.595381239893197 28.33600422548836 28.45349779865411 23.615116735964335 100-101 19.463513122503524 27.799821255219808 29.25935627909525 23.477309343181417 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4071.0 1 2731.0 2 1957.0 3 2601.5 4 2254.0 5 2132.0 6 3764.0 7 7631.5 8 11318.0 9 11443.5 10 8685.0 11 6599.0 12 6377.0 13 6519.5 14 6755.0 15 6985.5 16 7009.0 17 7075.5 18 7258.5 19 7530.0 20 8162.5 21 9243.0 22 10390.0 23 11695.0 24 13284.0 25 15578.5 26 19527.5 27 23962.5 28 26514.0 29 29885.5 30 35241.0 31 40347.5 32 47755.5 33 53710.5 34 57679.5 35 61299.5 36 65649.5 37 70770.5 38 70376.5 39 67856.0 40 66956.5 41 68436.5 42 69366.0 43 70576.5 44 72254.0 45 72613.0 46 71762.5 47 79938.0 48 105329.0 49 103989.5 50 79164.0 51 66468.0 52 59128.0 53 57764.0 54 57605.0 55 53068.5 56 46812.5 57 41837.0 58 39848.0 59 36620.0 60 31700.5 61 27505.5 62 23728.0 63 18337.0 64 12946.0 65 10219.5 66 7485.0 67 5859.5 68 4603.0 69 3098.0 70 2467.5 71 2134.0 72 1826.0 73 1853.5 74 1653.0 75 1402.5 76 1044.0 77 531.5 78 298.5 79 180.0 80 113.0 81 77.5 82 38.5 83 26.0 84 17.5 85 7.5 86 4.5 87 5.0 88 3.0 89 2.0 90 2.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2753929507280321 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002610968292572266 16-17 5.136331067355277E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.280275889462731E-5 26-27 0.0012198786284968784 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0036382345060433216 36-37 0.021058957376156637 38-39 0.007383475909323212 40-41 0.01827677804800586 42-43 0.005928182106905883 44-45 7.490482806559779E-4 46-47 1.0700689723656828E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.778372450774687E-4 60-61 0.002546764154230325 62-63 0.0014766951818646421 64-65 0.0 66-67 0.0 68-69 3.6382345060433216E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23293261390456182 78-79 0.0 80-81 0.0014552938024173286 82-83 0.0 84-85 0.0 86-87 0.10347566962776152 88-89 0.35515589192817015 90-91 0.35695360780174445 92-93 0.0 94-95 4.280275889462731E-5 96-97 0.0 98-99 0.0 100-101 2.5681655336776387E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2336298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.5997671379508 #Duplication Level Percentage of deduplicated Percentage of total 1 69.48597297206595 26.126564031036224 2 14.620861640253475 10.994819860594559 3 5.928815516248871 6.6876624844448145 4 3.0049320843468244 4.51938986547191 5 1.7188092969380557 3.2313414659707913 6 1.1162900526515662 2.5183347622865835 7 0.7723285924692329 2.0327562661577123 8 0.5193929758132037 1.5623243954931012 9 0.3859012068238191 1.305881596334681 >10 2.0593701156546595 14.451076831990967 >50 0.21579561788972382 5.643230209271012 >100 0.1545397361519972 10.809994936831608 >500 0.011744898304312235 2.9736338439142864 >1k 0.004447097416195893 3.5231968096033044 >5k 3.420844166304533E-4 0.7524013484846778 >10k+ 4.561125555072711E-4 2.867391292113896 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23152 0.9909694739284115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15691 0.6716180898155972 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15151 0.6485046000124983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12885 0.5515135483572728 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6366 0.27248236312319746 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5822 0.2491976622845202 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5361 0.22946559043409703 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4537 0.19419611710492413 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4526 0.19372528675708323 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4079 0.1745924535311848 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3635 0.15558802858197027 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3565 0.15259183545934638 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3462 0.14818315129319976 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3269 0.1399222188265367 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3249 0.13906616364864413 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2860 0.12241589043863411 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2563 0.10970347104692979 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.10863340207456411 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2514 0.10760613586109305 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2441 0.10448153446178526 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2391 0.10234139651705391 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.280275889462731E-5 0.0 18-19 0.0 0.0 0.0 1.0700689723656827E-4 0.0 20-21 0.0 0.0 0.0 2.7821793281507755E-4 0.0 22-23 0.0 0.0 0.0 8.560551778925463E-4 0.0 24-25 0.0 0.0 0.0 0.0019475255297055427 0.0 26-27 0.0 0.0 0.0 0.003424220711570185 0.0 28-29 0.0 0.0 0.0 0.006955448320376938 0.0 30-31 0.0 0.0 0.0 0.0194324525381608 0.0 32-33 0.0 0.0 0.0 0.044921495459911365 0.0 34-35 0.0 0.0 0.0 0.0817960722476328 0.0 36-37 0.0 0.0 0.0 0.14390287540373703 0.0 38-39 0.0 0.0 0.0 0.23682766496397292 0.0 40-41 0.0 0.0 0.0 0.34421978703059286 0.0 42-43 0.0 0.0 0.0 0.4479950759706167 0.0 44-45 0.0 0.0 0.0 0.5747554464370556 0.0 46-47 0.0 0.0 0.0 0.7303220736395786 0.0 48-49 0.0 0.0 0.0 0.8962683698740486 0.0 50-51 0.0 0.0 0.0 1.0867620483345874 0.0 52-53 0.0 0.0 0.0 1.2720551915894291 0.0 54-55 0.0 0.0 0.0 1.4808898522363156 0.0 56-57 0.0 0.0 0.0 1.7322704552244619 0.0 58-59 0.0 0.0 0.0 1.9996378886597515 0.0 60-61 0.0 0.0 0.0 2.299492616096063 0.0 62-63 0.0 0.0 0.0 2.602857169761734 0.0 64-65 0.0 0.0 0.0 2.9157239359020126 0.0 66-67 0.0 0.0 0.0 3.2858821948227495 0.0 68-69 0.0 0.0 0.0 3.704792796124467 0.0 70-71 0.0 0.0 0.0 4.16764042943152 0.0 72-73 0.0 0.0 0.0 4.628647544106103 0.0 74-75 0.0 0.0 0.0 5.11336738720831 0.0 76-77 0.0 0.0 0.0 5.632243831908429 0.0 78-79 0.0 0.0 0.0 6.239636382002638 0.0 80-81 0.0 0.0 0.0 6.895866879995617 0.0 82-83 0.0 0.0 0.0 7.546297604158374 0.0 84-85 0.0 0.0 0.0 8.210018584957911 0.0 86-87 0.0 0.0 0.0 8.909715284608385 0.0 88-89 0.0 0.0 0.0 9.652064933497353 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTAG 85 6.3425966E-4 27.991116 1 GTACATG 16830 0.0 26.83189 1 TACATGG 16765 0.0 26.60738 2 ACATGGG 16720 0.0 25.572094 3 ATTGCGT 95 0.0012338009 24.976074 6 TATATCG 120 1.7226994E-4 23.727272 5 GTAAGCG 120 2.3283064E-10 23.727272 94-95 CATGGGA 10390 0.0 23.110683 4 CATGGGG 5015 0.0 22.8993 4 CGTTAGA 105 0.002209153 22.597403 2 AGTACTT 16590 0.0 22.341875 12-13 GAGTACT 16470 0.0 21.409681 12-13 GTCGGGA 865 0.0 21.395689 2 ATGGGAT 3915 0.0 20.848482 5 CATGGGC 2410 0.0 20.084494 4 GTACTTT 17550 0.0 19.94343 14-15 GTATCAA 36165 0.0 19.69711 1 CGGGAGT 1000 0.0 19.456364 4 ATGGGAG 3735 0.0 19.185102 5 AGAGTAC 28195 0.0 19.121262 10-11 >>END_MODULE