##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939231_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1691 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.712004730928445 31.0 25.0 33.0 2.0 34.0 2 25.971023063276167 31.0 25.0 33.0 2.0 34.0 3 25.876404494382022 31.0 25.0 34.0 2.0 34.0 4 28.878769958604376 35.0 27.0 37.0 2.0 37.0 5 28.522767593140152 35.0 25.0 37.0 2.0 37.0 6 28.771141336487286 35.0 28.0 37.0 2.0 37.0 7 28.439384979302186 35.0 26.0 37.0 2.0 37.0 8 28.448255470136015 35.0 26.0 37.0 2.0 37.0 9 29.466587817859256 37.0 26.0 39.0 2.0 39.0 10-11 29.10348905972797 37.0 25.0 39.0 2.0 39.0 12-13 28.942046126552334 37.0 25.0 39.0 2.0 39.0 14-15 29.346540508574808 37.0 22.0 40.0 2.0 41.0 16-17 29.000591366055588 36.5 17.5 40.0 2.0 41.0 18-19 28.929036073329392 36.0 18.0 40.0 2.0 41.0 20-21 28.85600236546422 36.0 18.0 40.0 2.0 41.0 22-23 28.543465405085747 36.0 18.0 40.0 2.0 41.0 24-25 28.43524541691307 35.5 18.0 40.0 2.0 41.0 26-27 28.19574216439976 35.0 16.5 40.0 2.0 41.0 28-29 27.76167947959787 35.0 14.5 40.0 2.0 41.0 30-31 27.214665878178593 35.0 12.0 39.0 2.0 41.0 32-33 26.73920756948551 35.0 9.0 39.0 2.0 41.0 34-35 26.24600827912478 35.0 6.5 38.0 2.0 40.5 36-37 25.878769958604376 35.0 2.0 38.0 2.0 40.5 38-39 25.233589591957422 34.0 2.0 38.0 2.0 40.0 40-41 24.83619160260201 33.5 2.0 37.5 2.0 40.0 42-43 24.634831460674157 33.0 2.0 38.0 2.0 40.0 44-45 24.193672383205204 33.0 2.0 38.0 2.0 40.0 46-47 23.588113542282674 33.0 2.0 38.0 2.0 40.0 48-49 22.935836782968657 32.0 2.0 38.0 2.0 40.0 50-51 21.776167947959788 29.5 2.0 36.5 2.0 39.0 52-53 21.850680070963925 30.5 2.0 37.0 2.0 39.0 54-55 21.8672383205204 30.0 2.0 37.0 2.0 40.0 56-57 21.5204021289178 29.0 2.0 36.5 2.0 40.0 58-59 21.20195150798344 28.0 2.0 36.0 2.0 40.0 60-61 20.939976345357778 28.5 2.0 36.0 2.0 40.0 62-63 20.404790065050264 27.5 2.0 35.0 2.0 39.5 64-65 20.135718509757538 27.0 2.0 35.0 2.0 39.0 66-67 19.738320520402127 26.0 2.0 35.0 2.0 39.0 68-69 19.359846244825548 25.0 2.0 35.0 2.0 38.5 70-71 19.006209343583677 24.5 2.0 35.0 2.0 37.5 72-73 18.840331164991127 25.0 2.0 35.0 2.0 37.0 74-75 18.378474275576583 22.0 2.0 35.0 2.0 36.5 76-77 17.95623891188646 19.0 2.0 34.5 2.0 36.0 78-79 17.492607924305148 16.0 2.0 34.0 2.0 35.5 80-81 17.269071555292726 10.0 2.0 34.0 2.0 35.0 82-83 16.876700177409816 4.5 2.0 34.0 2.0 35.0 84-85 16.44736842105263 2.0 2.0 34.0 2.0 35.0 86-87 16.125073920756947 2.0 2.0 34.0 2.0 35.0 88-89 15.738911886457718 2.0 2.0 33.0 2.0 35.0 90-91 15.547309284447072 2.0 2.0 33.0 2.0 35.0 92-93 15.023063276167948 2.0 2.0 32.5 2.0 35.0 94-95 14.6428149024246 2.0 2.0 33.0 2.0 35.0 96-97 14.261088113542282 2.0 2.0 32.0 2.0 35.0 98-99 13.701655824955647 2.0 2.0 32.0 2.0 35.0 100-101 12.19189828503844 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 5.689964157706093 1102 2 6.369175627240146 1102 3 5.885304659498203 1102 4 7.026881720430104 1102 5 7.453405017921149 1102 6 7.4964157706093175 1102 7 6.894265232974902 1102 8 7.9372759856630815 1102 9 8.594982078853043 1102 10-11 6.773297491039429 1102 12-13 8.055555555555554 1102 14-15 9.820788530465947 1102 16-17 8.94534050179211 1102 18-19 10.627240143369178 1102 20-21 10.279569892473113 1102 22-23 11.74910394265233 1102 24-25 11.294802867383517 1102 26-27 10.870071684587813 1102 28-29 11.22043010752688 1102 30-31 12.113799283154119 1102 32-33 11.458781362007166 1102 34-35 13.140681003584227 1102 36-37 14.189068100358426 1102 38-39 15.842293906810038 1102 40-41 14.632616487455195 1102 42-43 12.741039426523297 1102 44-45 14.855734767025087 1102 46-47 15.672043010752684 1102 48-49 17.05734767025089 1102 50-51 18.183691756272403 1102 52-53 17.098566308243726 1102 54-55 16.419354838709676 1102 56-57 16.54032258064516 1102 58-59 14.924731182795693 1102 60-61 14.055555555555554 1102 62-63 14.26075268817204 1102 64-65 13.976702508960571 1102 66-67 12.942652329749109 1102 68-69 13.828853046594983 1102 70-71 14.609318996415773 1102 72-73 14.818100358422942 1102 74-75 15.442652329749105 1102 76-77 15.191756272401435 1102 78-79 14.93100358422939 1102 80-81 15.097670250896055 1102 82-83 12.793906810035846 1102 84-85 15.013440860215052 1102 86-87 13.517025089605735 1102 88-89 14.782258064516132 1102 90-91 14.151433691756274 1102 92-93 15.357526881720428 1102 94-95 18.259856630824373 1102 96-97 14.570788530465949 1102 98-99 12.554659498207881 1102 100-101 14.796594982078853 1103 1 6.689964157706093 1103 2 5.869175627240146 1103 3 3.8853046594982033 1103 4 7.026881720430104 1103 5 7.453405017921149 1103 6 7.9964157706093175 1103 7 8.894265232974902 1103 8 8.937275985663081 1103 9 9.594982078853043 1103 10-11 9.273297491039429 1103 12-13 9.555555555555554 1103 14-15 10.070788530465947 1103 16-17 9.44534050179211 1103 18-19 10.377240143369178 1103 20-21 11.029569892473113 1103 22-23 10.74910394265233 1103 24-25 9.794802867383517 1103 26-27 9.620071684587813 1103 28-29 10.97043010752688 1103 30-31 11.613799283154119 1103 32-33 12.458781362007166 1103 34-35 12.640681003584227 1103 36-37 13.439068100358426 1103 38-39 10.842293906810038 1103 40-41 11.632616487455195 1103 42-43 13.241039426523297 1103 44-45 13.105734767025087 1103 46-47 12.172043010752684 1103 48-49 13.057347670250891 1103 50-51 12.683691756272403 1103 52-53 12.848566308243726 1103 54-55 13.919354838709676 1103 56-57 14.29032258064516 1103 58-59 14.424731182795693 1103 60-61 14.805555555555554 1103 62-63 15.51075268817204 1103 64-65 15.976702508960571 1103 66-67 16.94265232974911 1103 68-69 15.078853046594983 1103 70-71 17.109318996415773 1103 72-73 17.068100358422942 1103 74-75 17.192652329749105 1103 76-77 17.191756272401435 1103 78-79 17.18100358422939 1103 80-81 13.847670250896055 1103 82-83 16.543906810035846 1103 84-85 17.513440860215052 1103 86-87 18.017025089605735 1103 88-89 17.28225806451613 1103 90-91 17.401433691756274 1103 92-93 18.10752688172043 1103 94-95 16.509856630824373 1103 96-97 18.320788530465947 1103 98-99 18.804659498207883 1103 100-101 4.296594982078853 1104 1 7.689964157706093 1104 2 7.869175627240146 1104 3 7.385304659498203 1104 4 8.026881720430104 1104 5 8.453405017921149 1104 6 7.9964157706093175 1104 7 6.894265232974902 1104 8 7.9372759856630815 1104 9 9.594982078853043 1104 10-11 10.023297491039429 1104 12-13 9.805555555555554 1104 14-15 11.320788530465947 1104 16-17 11.44534050179211 1104 18-19 11.627240143369178 1104 20-21 11.529569892473113 1104 22-23 11.99910394265233 1104 24-25 8.794802867383517 1104 26-27 6.120071684587813 1104 28-29 6.47043010752688 1104 30-31 6.863799283154119 1104 32-33 7.958781362007166 1104 34-35 8.390681003584227 1104 36-37 0.6890681003584262 1104 38-39 -19.40770609318996 1104 40-41 -18.117383512544805 1104 42-43 -10.008960573476703 1104 44-45 -0.644265232974913 1104 46-47 -14.577956989247316 1104 48-49 -16.44265232974911 1104 50-51 -14.066308243727597 1104 52-53 -1.4014336917562744 1104 54-55 6.919354838709676 1104 56-57 12.54032258064516 1104 58-59 12.674731182795693 1104 60-61 -2.4444444444444464 1104 62-63 11.01075268817204 1104 64-65 12.976702508960571 1104 66-67 -18.80734767025089 1104 68-69 -18.421146953405017 1104 70-71 -18.140681003584227 1104 72-73 -17.931899641577058 1104 74-75 -17.307347670250895 1104 76-77 -17.058243727598565 1104 78-79 -17.06899641577061 1104 80-81 -16.652329749103945 1104 82-83 -16.706093189964154 1104 84-85 -15.986559139784948 1104 86-87 -15.482974910394265 1104 88-89 -14.717741935483868 1104 90-91 -14.848566308243726 1104 92-93 -14.392473118279572 1104 94-95 -13.990143369175627 1104 96-97 -12.929211469534051 1104 98-99 -13.195340501792119 1104 100-101 -11.953405017921147 1105 1 -23.310035842293907 1105 2 -24.130824372759854 1105 3 -24.614695340501797 1105 4 -26.973118279569896 1105 5 -26.54659498207885 1105 6 -27.003584229390682 1105 7 -26.105734767025098 1105 8 -26.06272401433692 1105 9 -27.405017921146957 1105 10-11 -26.97670250896057 1105 12-13 -27.194444444444446 1105 14-15 -27.679211469534053 1105 16-17 -27.55465949820789 1105 18-19 -27.37275985663082 1105 20-21 -27.470430107526887 1105 22-23 -27.00089605734767 1105 24-25 -27.205197132616483 1105 26-27 -26.879928315412187 1105 28-29 -26.52956989247312 1105 30-31 -26.13620071684588 1105 32-33 -25.041218637992834 1105 34-35 -24.609318996415773 1105 36-37 -23.810931899641574 1105 38-39 -22.40770609318996 1105 40-41 -22.617383512544805 1105 42-43 -22.508960573476703 1105 44-45 -22.144265232974913 1105 46-47 -22.077956989247316 1105 48-49 -21.44265232974911 1105 50-51 -20.566308243727597 1105 52-53 -20.401433691756274 1105 54-55 -21.080645161290324 1105 56-57 -20.45967741935484 1105 58-59 -20.325268817204307 1105 60-61 -20.444444444444446 1105 62-63 -19.98924731182796 1105 64-65 -19.02329749103943 1105 66-67 -18.80734767025089 1105 68-69 -18.421146953405017 1105 70-71 -18.140681003584227 1105 72-73 -17.931899641577058 1105 74-75 -17.307347670250895 1105 76-77 -17.058243727598565 1105 78-79 -17.06899641577061 1105 80-81 -16.652329749103945 1105 82-83 -16.706093189964154 1105 84-85 -15.986559139784948 1105 86-87 -15.482974910394265 1105 88-89 -14.717741935483868 1105 90-91 -14.848566308243726 1105 92-93 -14.392473118279572 1105 94-95 -13.990143369175627 1105 96-97 -12.929211469534051 1105 98-99 -13.195340501792119 1105 100-101 -11.953405017921147 1106 1 8.689964157706093 1106 2 7.869175627240146 1106 3 7.385304659498203 1106 4 8.026881720430104 1106 5 8.453405017921149 1106 6 7.9964157706093175 1106 7 8.894265232974902 1106 8 8.937275985663081 1106 9 9.594982078853043 1106 10-11 9.523297491039429 1106 12-13 9.805555555555554 1106 14-15 9.820788530465947 1106 16-17 10.94534050179211 1106 18-19 11.127240143369178 1106 20-21 11.529569892473113 1106 22-23 11.49910394265233 1106 24-25 11.294802867383517 1106 26-27 11.120071684587813 1106 28-29 9.47043010752688 1106 30-31 6.863799283154119 1106 32-33 8.458781362007166 1106 34-35 5.890681003584227 1106 36-37 13.689068100358426 1106 38-39 16.09229390681004 1106 40-41 15.882616487455195 1106 42-43 14.491039426523297 1106 44-45 13.855734767025087 1106 46-47 15.422043010752684 1106 48-49 15.057347670250891 1106 50-51 15.933691756272403 1106 52-53 16.098566308243726 1106 54-55 16.919354838709676 1106 56-57 17.04032258064516 1106 58-59 17.674731182795693 1106 60-61 16.555555555555554 1106 62-63 17.01075268817204 1106 64-65 15.976702508960571 1106 66-67 16.19265232974911 1106 68-69 16.078853046594983 1106 70-71 13.859318996415773 1106 72-73 16.068100358422942 1106 74-75 14.692652329749105 1106 76-77 15.941756272401435 1106 78-79 15.93100358422939 1106 80-81 15.347670250896055 1106 82-83 16.293906810035846 1106 84-85 17.013440860215052 1106 86-87 17.517025089605735 1106 88-89 18.28225806451613 1106 90-91 18.151433691756274 1106 92-93 18.60752688172043 1106 94-95 18.509856630824373 1106 96-97 19.570788530465947 1106 98-99 19.804659498207883 1106 100-101 20.54659498207885 1107 1 5.689964157706093 1107 2 -4.130824372759854 1107 3 4.385304659498203 1107 4 5.526881720430104 1107 5 6.453405017921149 1107 6 6.9964157706093175 1107 7 6.394265232974902 1107 8 -4.5627240143369185 1107 9 -1.405017921146957 1107 10-11 -0.726702508960571 1107 12-13 0.8055555555555536 1107 14-15 -1.929211469534053 1107 16-17 1.19534050179211 1107 18-19 8.627240143369178 1107 20-21 8.029569892473113 1107 22-23 5.249103942652329 1107 24-25 9.294802867383517 1107 26-27 7.870071684587813 1107 28-29 6.47043010752688 1107 30-31 10.363799283154119 1107 32-33 -0.5412186379928343 1107 34-35 2.140681003584227 1107 36-37 7.939068100358426 1107 38-39 3.8422939068100384 1107 40-41 3.632616487455195 1107 42-43 2.7410394265232974 1107 44-45 0.855734767025087 1107 46-47 8.672043010752684 1107 48-49 9.557347670250891 1107 50-51 9.933691756272403 1107 52-53 12.098566308243726 1107 54-55 6.669354838709676 1107 56-57 6.54032258064516 1107 58-59 1.1747311827956928 1107 60-61 11.055555555555554 1107 62-63 11.26075268817204 1107 64-65 10.476702508960571 1107 66-67 0.6926523297491087 1107 68-69 8.328853046594983 1107 70-71 2.859318996415773 1107 72-73 5.318100358422942 1107 74-75 1.1926523297491052 1107 76-77 4.441756272401435 1107 78-79 9.18100358422939 1107 80-81 10.847670250896055 1107 82-83 14.043906810035846 1107 84-85 13.763440860215052 1107 86-87 15.517025089605735 1107 88-89 1.7822580645161317 1107 90-91 1.6514336917562744 1107 92-93 2.1075268817204282 1107 94-95 2.0098566308243733 1107 96-97 3.570788530465949 1107 98-99 3.304659498207881 1107 100-101 4.546594982078853 1108 1 7.189964157706093 1108 2 7.869175627240146 1108 3 7.385304659498203 1108 4 8.026881720430104 1108 5 8.453405017921149 1108 6 7.9964157706093175 1108 7 8.894265232974902 1108 8 8.937275985663081 1108 9 9.094982078853043 1108 10-11 10.023297491039429 1108 12-13 9.555555555555554 1108 14-15 8.820788530465947 1108 16-17 9.44534050179211 1108 18-19 10.377240143369178 1108 20-21 10.029569892473113 1108 22-23 7.999103942652329 1108 24-25 9.044802867383517 1108 26-27 9.620071684587813 1108 28-29 8.47043010752688 1108 30-31 8.613799283154119 1108 32-33 10.708781362007166 1108 34-35 9.390681003584227 1108 36-37 11.189068100358426 1108 38-39 12.842293906810038 1108 40-41 12.132616487455195 1108 42-43 13.491039426523297 1108 44-45 13.855734767025087 1108 46-47 12.672043010752684 1108 48-49 12.807347670250891 1108 50-51 13.933691756272403 1108 52-53 14.598566308243726 1108 54-55 13.169354838709676 1108 56-57 14.29032258064516 1108 58-59 14.924731182795693 1108 60-61 13.555555555555554 1108 62-63 14.01075268817204 1108 64-65 14.726702508960571 1108 66-67 15.692652329749109 1108 68-69 17.578853046594983 1108 70-71 17.859318996415773 1108 72-73 17.568100358422942 1108 74-75 18.692652329749105 1108 76-77 17.441756272401435 1108 78-79 16.93100358422939 1108 80-81 15.097670250896055 1108 82-83 15.543906810035846 1108 84-85 18.013440860215052 1108 86-87 11.017025089605735 1108 88-89 16.78225806451613 1108 90-91 15.151433691756274 1108 92-93 16.10752688172043 1108 94-95 16.259856630824373 1108 96-97 17.570788530465947 1108 98-99 18.554659498207883 1108 100-101 18.29659498207885 1109 1 4.189964157706093 1109 2 5.869175627240146 1109 3 -4.614695340501797 1109 4 3.5268817204301044 1109 5 6.453405017921149 1109 6 5.9964157706093175 1109 7 5.394265232974902 1109 8 6.9372759856630815 1109 9 -1.905017921146957 1109 10-11 5.273297491039429 1109 12-13 4.305555555555554 1109 14-15 -1.429211469534053 1109 16-17 -1.05465949820789 1109 18-19 0.12724014336917833 1109 20-21 6.029569892473113 1109 22-23 7.249103942652329 1109 24-25 -4.205197132616483 1109 26-27 6.620071684587813 1109 28-29 4.72043010752688 1109 30-31 1.113799283154119 1109 32-33 6.208781362007166 1109 34-35 8.890681003584227 1109 36-37 6.189068100358426 1109 38-39 12.092293906810038 1109 40-41 14.632616487455195 1109 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-17.307347670250895 2315 76-77 -17.058243727598565 2315 78-79 -17.06899641577061 2315 80-81 -16.652329749103945 2315 82-83 -16.706093189964154 2315 84-85 -15.986559139784948 2315 86-87 -15.482974910394265 2315 88-89 -14.717741935483868 2315 90-91 -14.848566308243726 2315 92-93 -14.392473118279572 2315 94-95 -13.990143369175627 2315 96-97 -12.929211469534051 2315 98-99 -13.195340501792119 2315 100-101 -11.953405017921147 2316 1 -2.9767025089605745 2316 2 -3.464157706093186 2316 3 -4.281362007168465 2316 4 -3.6397849462365635 2316 5 -3.879928315412183 2316 6 -4.336917562724015 2316 7 -2.7724014336917655 2316 8 -2.7293906810035864 2316 9 -4.071684587813625 2316 10-11 -3.476702508960571 2316 12-13 -3.1944444444444464 2316 14-15 -2.3458781362007173 2316 16-17 -2.721326164874558 2316 18-19 -2.2060931899641574 2316 20-21 -1.6370967741935516 2316 22-23 -2.167562724014335 2316 24-25 -2.038530465949819 2316 26-27 -1.8799283154121866 2316 28-29 -1.362903225806452 2316 30-31 -1.9695340501792131 2316 32-33 -2.207885304659502 2316 34-35 -1.4426523297491087 2316 36-37 -0.31093189964157375 2316 38-39 -0.7410394265232974 2316 40-41 -3.784050179211473 2316 42-43 0.9910394265232974 2316 44-45 1.855734767025087 2316 46-47 1.9220430107526845 2316 48-49 1.5573476702508913 2316 50-51 -2.5663082437275975 2316 52-53 1.7652329749103934 2316 54-55 2.752688172043012 2316 56-57 2.7069892473118244 2316 58-59 -2.4919354838709715 2316 60-61 -8.111111111111114 2316 62-63 -7.322580645161294 2316 64-65 -6.523297491039429 2316 66-67 -5.974014336917559 2316 68-69 -6.2544802867383495 2316 70-71 -5.974014336917559 2316 72-73 -6.598566308243726 2316 74-75 -5.974014336917563 2316 76-77 -6.058243727598565 2316 78-79 -6.4023297491039415 2316 80-81 -6.652329749103945 2316 82-83 -5.872759856630822 2316 84-85 -5.319892473118282 2316 86-87 -4.649641577060933 2316 88-89 -3.7177419354838683 2316 90-91 -3.8485663082437256 2316 92-93 -3.3924731182795718 2316 94-95 -2.9901433691756267 2316 96-97 -1.9292114695340512 2316 98-99 -2.195340501792119 2316 100-101 -1.1200716845878151 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 199.0 3 41.0 4 37.0 5 30.0 6 29.0 7 34.0 8 23.0 9 33.0 10 30.0 11 25.0 12 19.0 13 25.0 14 27.0 15 29.0 16 31.0 17 40.0 18 40.0 19 27.0 20 25.0 21 27.0 22 23.0 23 32.0 24 21.0 25 20.0 26 28.0 27 30.0 28 27.0 29 28.0 30 43.0 31 43.0 32 46.0 33 71.0 34 88.0 35 135.0 36 113.0 37 101.0 38 64.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.786982248520715 19.112426035502956 15.621301775147927 21.4792899408284 2 15.976331360946746 22.781065088757398 37.514792899408285 23.727810650887573 3 22.189349112426036 25.62130177514793 29.467455621301774 22.72189349112426 4 13.491124260355031 18.284023668639055 35.91715976331361 32.30769230769231 5 14.082840236686389 37.81065088757396 31.301775147928996 16.80473372781065 6 28.326434062684804 36.1324659964518 17.14961561206387 18.391484328799525 7 26.138379657007686 34.594914251921935 20.40212891780012 18.864577173270256 8 27.61679479597871 31.105854523950327 18.154937906564165 23.122412773506802 9 26.31578947368421 15.789473684210526 21.762270845653457 36.1324659964518 10-11 23.388527498521587 27.823772915434652 25.133057362507394 23.654642223536367 12-13 27.942046126552334 24.659964518036663 27.321111768184508 20.076877587226495 14-15 23.388527498521587 26.34535777646363 27.70549970431697 22.56061502069781 16-17 20.5204021289178 29.775280898876407 28.00118273211118 21.703134240094617 18-19 20.195150798344173 25.931401537551746 32.79124778237729 21.08219988172679 20-21 19.75162625665287 26.04967474866943 33.44175044352454 20.756948551153165 22-23 20.40212891780012 25.044352454169132 33.530455351862805 21.023063276167946 24-25 19.396806623299824 25.517445298639856 33.32347723240686 21.762270845653457 26-27 20.076877587226495 26.581904198698997 33.944411590774685 19.396806623299824 28-29 18.450620934358366 26.7297457125961 35.09757539917209 19.72205795387345 30-31 19.544648137196923 27.261975162625667 33.50088704908338 19.69248965109403 32-33 19.101123595505616 25.960969840331167 33.826138379657 21.11176818450621 34-35 20.79383886255924 26.74763033175355 32.49407582938388 19.964454976303315 36-37 20.00592768227623 26.733847065797274 32.24659158269117 21.01363366923533 38-39 20.657972732661527 27.622999407231774 32.483698873740366 19.235328986366333 40-41 19.531713100177832 27.682276229994073 31.653823355068166 21.13218731475993 42-43 18.761114404267932 28.77889745109662 31.742738589211616 20.71724955542383 44-45 19.70954356846473 27.415530527563725 32.483698873740366 20.391227030231178 46-47 19.122442929143197 29.41002075303884 30.388378298250817 21.079158019567153 48-49 20.74688796680498 27.711914641375223 30.02371072910492 21.51748666271488 50-51 20.077105575326215 29.270462633451956 30.041518386714117 20.61091340450771 52-53 20.13641755634638 28.973902728351124 30.308422301304866 20.581257413997626 54-55 19.85773562537048 28.24540604623592 29.63841138114997 22.258446947243627 56-57 19.88737403675163 28.39359810314167 29.164196799051574 22.55483106105513 58-59 21.01363366923533 29.134558387670424 28.74925903971547 21.10254890337878 60-61 22.40663900414938 25.903971547125078 29.81624184943687 21.87314759928868 62-63 18.731475992886782 29.045643153526974 30.14226437462952 22.080616478956728 64-65 19.205690574985184 27.889745109662123 31.535269709543567 21.369294605809127 66-67 19.329973317521496 27.601541654313667 30.56626148828936 22.502223539875484 68-69 19.72123368920522 28.202846975088967 30.01186239620403 22.064056939501782 70-71 19.804328490957605 28.431663207826862 28.90601838126297 22.857989919952566 72-73 19.205690574985184 28.838174273858918 30.52756372258447 21.428571428571427 74-75 19.531713100177832 28.63070539419087 29.549496147006522 22.28808535862478 76-77 20.21339656194428 27.208061647895672 30.73503260225252 21.84350918790753 78-79 20.09484291641968 28.33432128037937 30.05334914048607 21.51748666271488 80-81 19.353882631890933 28.71962062833432 29.608772969768822 22.31772377000593 82-83 21.07291049199763 26.763485477178424 29.312388855957323 22.851215174866628 84-85 20.00592768227623 27.415530527563725 29.87551867219917 22.70302311796088 86-87 19.65026674570243 28.15649081209247 29.99407231772377 22.199170124481327 88-89 19.65026674570243 27.208061647895672 28.54179016004742 24.599881446354473 90-91 19.21138452416247 28.04624962941002 29.736139934776162 23.00622591165135 92-93 20.575155647791284 27.690483249332935 29.350726356359324 22.383634746516453 94-95 21.345982804624963 25.55588496887044 29.2321375630003 23.8659946635043 96-97 19.97628927089508 27.415530527563725 29.608772969768822 22.999407231772377 98-99 20.160094871034687 28.016602431070265 27.601541654313667 24.221761043581385 100-101 20.98399525785418 26.615293420272675 29.075281564908124 23.325429756965026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25.0 1 13.5 2 8.0 3 10.5 4 5.5 5 5.0 6 8.0 7 15.0 8 17.5 9 12.5 10 9.0 11 9.0 12 9.0 13 7.0 14 8.0 15 9.0 16 6.5 17 6.0 18 7.0 19 8.0 20 12.5 21 10.5 22 9.5 23 12.5 24 10.5 25 8.0 26 9.0 27 16.5 28 21.0 29 17.5 30 23.0 31 27.0 32 20.5 33 25.0 34 26.0 35 24.0 36 29.5 37 26.5 38 29.0 39 36.0 40 29.0 41 33.5 42 38.5 43 32.5 44 43.5 45 46.0 46 42.5 47 48.0 48 53.5 49 60.0 50 51.5 51 44.5 52 50.5 53 53.5 54 49.5 55 51.0 56 46.5 57 43.0 58 41.0 59 36.0 60 36.0 61 30.0 62 27.5 63 24.5 64 20.0 65 16.5 66 14.0 67 10.0 68 5.5 69 7.0 70 8.0 71 5.0 72 2.5 73 2.5 74 3.0 75 1.5 76 0.0 77 0.5 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05913660555884093 2 0.05913660555884093 3 0.05913660555884093 4 0.05913660555884093 5 0.05913660555884093 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.17740981667652278 36-37 0.2365464222353637 38-39 0.2365464222353637 40-41 0.2365464222353637 42-43 0.2365464222353637 44-45 0.2365464222353637 46-47 0.26611472501478417 48-49 0.2365464222353637 50-51 0.29568302779420463 52-53 0.29568302779420463 54-55 0.2365464222353637 56-57 0.2365464222353637 58-59 0.2365464222353637 60-61 0.2365464222353637 62-63 0.2365464222353637 64-65 0.2365464222353637 66-67 0.26611472501478417 68-69 0.29568302779420463 70-71 0.26611472501478417 72-73 0.2365464222353637 74-75 0.2365464222353637 76-77 0.2365464222353637 78-79 0.2365464222353637 80-81 0.2365464222353637 82-83 0.2365464222353637 84-85 0.2365464222353637 86-87 0.2365464222353637 88-89 0.2365464222353637 90-91 0.26611472501478417 92-93 0.26611472501478417 94-95 0.26611472501478417 96-97 0.2365464222353637 98-99 0.26611472501478417 100-101 0.2365464222353637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1691.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.8929627439385 #Duplication Level Percentage of deduplicated Percentage of total 1 98.11320754716981 89.17800118273212 2 0.8458035133376708 1.537551744529864 3 0.3903708523096942 1.0644589000591367 4 0.06506180871828238 0.2365464222353637 5 0.0 0.0 6 0.1951854261548471 1.0644589000591367 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.3903708523096942 6.9189828503843875 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 2.0106445890005915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 1.360141927853341 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 1.360141927853341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.827912477823773 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.768775872264932 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.5913660555884093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6 0.35481963335304556 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.35481963335304556 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.35481963335304556 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2365464222353637 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3 0.17740981667652278 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3 0.17740981667652278 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3 0.17740981667652278 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.17740981667652278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 3 0.17740981667652278 No Hit GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA 3 0.17740981667652278 No Hit GCCTATATCTACATGATAATACATAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11827321111768185 No Hit GTTCTAACTGGGTGCTGGGACTGCACAAAGCCCTGCAGCTCACACGACTG 2 0.11827321111768185 No Hit CTCCGGGACCATCTCCTCGCTGCCTTCAGCTCCTAGGACCAGTCTGCACC 2 0.11827321111768185 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2 0.11827321111768185 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2 0.11827321111768185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA 2 0.11827321111768185 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 2 0.11827321111768185 No Hit AAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCG 2 0.11827321111768185 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 2 0.11827321111768185 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGG 2 0.11827321111768185 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2 0.11827321111768185 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11827321111768185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 2 0.11827321111768185 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.02956830277942046 0.0 28-29 0.0 0.0 0.0 0.05913660555884092 0.0 30-31 0.0 0.0 0.0 0.05913660555884092 0.0 32-33 0.0 0.0 0.0 0.11827321111768184 0.0 34-35 0.0 0.0 0.0 0.11827321111768184 0.0 36-37 0.0 0.0 0.0 0.11827321111768184 0.0 38-39 0.0 0.0 0.0 0.11827321111768184 0.0 40-41 0.0 0.0 0.0 0.11827321111768184 0.0 42-43 0.0 0.0 0.0 0.17740981667652278 0.0 44-45 0.0 0.0 0.0 0.23654642223536368 0.0 46-47 0.0 0.0 0.0 0.23654642223536368 0.0 48-49 0.0 0.0 0.0 0.26611472501478417 0.0 50-51 0.0 0.0 0.0 0.29568302779420463 0.0 52-53 0.0 0.0 0.0 0.35481963335304556 0.0 54-55 0.0 0.0 0.0 0.384387936132466 0.0 56-57 0.0 0.0 0.0 0.4435245416913069 0.0 58-59 0.0 0.0 0.0 0.5322294500295683 0.0 60-61 0.0 0.0 0.0 0.6505026611472502 0.0 62-63 0.0 0.0 0.0 0.6505026611472502 0.0 64-65 0.0 0.0 0.0 0.7983441750443525 0.0 66-67 0.0 0.0 0.0 0.8870490833826138 0.0 68-69 0.0 0.0 0.0 0.9461856889414547 0.0 70-71 0.0 0.0 0.0 1.0348905972797162 0.0 72-73 0.0 0.0 0.0 1.2418687167356595 0.0 74-75 0.0 0.0 0.0 1.27143701951508 0.0 76-77 0.0 0.0 0.0 1.4488468361916027 0.0 78-79 0.0 0.0 0.0 1.5671200473092846 0.0 80-81 0.0 0.0 0.0 1.9515079834417506 0.0 82-83 0.0 0.0 0.0 2.0697811945594324 0.0 84-85 0.0 0.0 0.0 2.424600827912478 0.0 86-87 0.0 0.0 0.0 2.5133057362507394 0.0 88-89 0.0 0.0 0.0 2.7202838557066826 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE