FastQCFastQC Report
Fri 27 May 2016
SRR939231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382.247191011235955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281.655824955647546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171.0053222945002958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.9461856889414548No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.5913660555884093No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC90.5322294500295683No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA80.4730928444707274No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA80.4730928444707274No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70.4139562389118865No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA60.35481963335304556No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT50.29568302779420463No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.29568302779420463No Hit
GTGTGGACTCGGCCCAGTCTTTCTGAGGCAGGCAGGCAGGGGCCACGTTC40.2365464222353637No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2365464222353637No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2365464222353637No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT40.2365464222353637No Hit
GTCGTAGCGCACGTACTCCTCCCGGTTGTAGATGTATCTGGTCACATCTC40.2365464222353637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2365464222353637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT30.17740981667652278No Hit
GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT30.17740981667652278No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30.17740981667652278No Hit
GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC30.17740981667652278No Hit
ACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTT30.17740981667652278No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.17740981667652278No Hit
TTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT30.17740981667652278No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30.17740981667652278No Hit
GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA30.17740981667652278No Hit
GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA30.17740981667652278No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30.17740981667652278No Hit
CTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATG30.17740981667652278No Hit
GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT30.17740981667652278No Hit
ACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTT20.11827321111768185No Hit
AGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.11827321111768185TruSeq Adapter, Index 3 (96% over 28bp)
GTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTAT20.11827321111768185No Hit
TAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGAT20.11827321111768185No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT20.11827321111768185No Hit
GTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11827321111768185No Hit
GTCTTTCATCTTTCCCTTGCGGTACTAGTTCTATAGCTCCTAGATGTACG20.11827321111768185No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11827321111768185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT20.11827321111768185No Hit
CCTAATAAGCTGTGTGGATGAGACCCCCACCGTCTCCTCCTGGCCATTCC20.11827321111768185No Hit
GTCGTAGGTGTCCCTCTGAAACTTGTTGTGCTTAATGACCACGTCCACCT20.11827321111768185No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG20.11827321111768185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.11827321111768185No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA20.11827321111768185No Hit
GTTCAAGCCGCCGCAGGGAGGTGTGGGTCTCCGGCCCCTCGTAGTTGTGT20.11827321111768185No Hit
GATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAGA20.11827321111768185No Hit
CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT20.11827321111768185No Hit
CTCCGGCCCCTCGTAGTTGTGTCTGCACACCGTGTCCAGCTCGGCCCGCG20.11827321111768185No Hit
CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA20.11827321111768185No Hit
ACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTA20.11827321111768185No Hit
TTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC20.11827321111768185No Hit
TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATA20.11827321111768185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.11827321111768185No Hit
CCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCT20.11827321111768185No Hit
GCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACG20.11827321111768185No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT20.11827321111768185No Hit
TCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGAC20.11827321111768185No Hit
ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA20.11827321111768185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA20.11827321111768185No Hit
GTACATGGGAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11827321111768185No Hit
GTTCTATAATCGATAAACCCCGCTCTACCTCACCATCTCTTGCTAATTCA20.11827321111768185No Hit
GCTATGAAGTTATCAGGGAGTAACTGCCCATTTGAAAGAAACTCGTCATC20.11827321111768185No Hit
GGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGAGGTGT20.11827321111768185No Hit
CTGTAGGATCAGCTTGGTATTTCAAGGGATCCTCCTTGCCACGGTGGTCA20.11827321111768185No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT20.11827321111768185No Hit
GACCAGGACCTGGAAGGTCCAGTCCCCATTCCTAATAAGCTGTGTGGATG20.11827321111768185No Hit
ATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTC20.11827321111768185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA20.11827321111768185No Hit
ATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCA20.11827321111768185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA201.2763348E-595.000011
AACGCAG309.54892E-563.3333367
GTATCAA309.54892E-563.3333362
TCAACGC309.54892E-563.3333365
TATCAAC309.54892E-563.3333363
ATCAACG309.54892E-563.3333364
CAACGCA309.54892E-563.3333366
ACGCAGA352.0485882E-454.285718
CGCAGAG352.0485882E-454.285719
CAGAGTA353.1462878E-640.71428310-11
GAGTACT250.001393405538.012-13
GTACTTT300.003411531231.66666814-15
AGAGTAC350.00725521727.14285512-13
GCAGAGT350.00725521727.1428559
Produced by FastQC (version 0.11.5)