##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939231_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1691 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.150798344175044 31.0 31.0 33.0 30.0 34.0 2 31.33116499112951 31.0 31.0 34.0 30.0 34.0 3 31.481371969248965 31.0 31.0 34.0 30.0 34.0 4 35.28622117090479 35.0 35.0 37.0 33.0 37.0 5 35.03666469544648 35.0 35.0 37.0 32.0 37.0 6 35.15552927261975 37.0 35.0 37.0 32.0 37.0 7 34.90715552927262 36.0 35.0 37.0 32.0 37.0 8 34.82436428149024 35.0 35.0 37.0 30.0 37.0 9 36.31460674157304 38.0 35.0 39.0 30.0 39.0 10-11 36.264044943820224 38.0 35.0 39.0 32.0 39.0 12-13 36.08338261383797 38.0 35.0 39.0 30.0 39.0 14-15 36.852454169130695 38.0 35.5 40.0 31.0 41.0 16-17 36.841218214074516 38.0 36.0 40.0 31.0 41.0 18-19 37.04612655233589 38.5 36.0 40.0 31.5 41.0 20-21 37.036073329390895 39.0 35.5 40.0 31.0 41.0 22-23 36.95505617977528 38.5 35.0 40.0 31.0 41.0 24-25 36.85866351271437 38.5 35.0 40.0 30.5 41.0 26-27 36.3926670609107 38.0 35.0 40.0 30.0 41.0 28-29 36.14399763453578 38.0 35.0 40.0 30.0 41.0 30-31 35.67090479006505 37.5 34.5 40.0 29.0 41.0 32-33 35.0665286812537 37.0 33.5 40.0 27.0 41.0 34-35 34.89444115907747 37.0 33.5 40.0 27.0 41.0 36-37 34.678296865759904 37.0 33.5 40.0 26.0 41.0 38-39 34.19041986989947 36.5 33.0 40.0 24.0 41.0 40-41 33.76759314015376 36.0 33.0 40.0 23.5 41.0 42-43 33.3574807806032 36.0 33.0 40.0 21.0 41.0 44-45 32.92164399763453 36.0 32.0 39.5 18.5 41.0 46-47 31.8214074512123 35.5 30.0 39.5 10.5 41.0 48-49 31.12448255470136 35.0 29.5 39.0 4.0 41.0 50-51 30.722057953873446 35.0 29.0 39.0 2.0 40.0 52-53 30.16499112950917 35.0 28.0 39.0 2.0 40.0 54-55 29.928444707273805 35.0 27.5 39.0 2.0 40.0 56-57 29.30751034890597 35.0 26.0 38.0 2.0 40.0 58-59 28.878769958604376 34.0 25.5 38.0 2.0 40.0 60-61 28.401537551744532 34.0 25.5 38.0 2.0 40.0 62-63 27.96836191602602 34.0 23.5 37.0 2.0 40.0 64-65 27.539030159668833 33.5 23.5 37.0 2.0 39.5 66-67 27.25813128326434 33.0 22.5 36.5 2.0 39.0 68-69 26.668539325842694 33.0 21.0 36.0 2.0 39.0 70-71 26.01892371377883 32.0 19.0 35.0 2.0 39.0 72-73 25.64399763453578 32.0 19.5 35.0 2.0 38.0 74-75 25.05499704316972 31.0 13.5 35.0 2.0 37.0 76-77 23.412477823772917 29.5 9.0 33.5 2.0 35.5 78-79 23.91632170313424 30.5 7.0 34.0 2.0 36.0 80-81 23.824659964518034 30.5 4.0 35.0 2.0 36.0 82-83 23.37995269071555 31.0 2.0 34.0 2.0 35.0 84-85 22.75162625665287 30.0 2.0 34.0 2.0 35.0 86-87 22.052335895919576 29.5 2.0 34.0 2.0 35.0 88-89 21.181253696037846 29.0 2.0 34.0 2.0 35.0 90-91 20.55410999408634 28.5 2.0 34.0 2.0 35.0 92-93 19.941454760496747 27.0 2.0 34.0 2.0 35.0 94-95 19.045535186280308 26.0 2.0 33.0 2.0 35.0 96-97 18.06179775280899 24.5 2.0 33.0 2.0 34.5 98-99 17.05913660555884 20.5 2.0 33.0 2.0 34.0 100-101 14.14488468361916 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 1.2956989247311839 1102 2 -0.5967741935483808 1102 3 1.1810035842293907 1102 4 -0.05376344086020879 1102 5 -0.04301075268817556 1102 6 -0.2831541218637952 1102 7 -0.032258064516128115 1102 8 1.1290322580645196 1102 9 2.6433691756272353 1102 10-11 0.9946236559139763 1102 12-13 0.7849462365591364 1102 14-15 1.7634408602150557 1102 16-17 0.14516129032258362 1102 18-19 2.1057347670250834 1102 20-21 3.1353046594982033 1102 22-23 0.7392473118279597 1102 24-25 2.7697132616487394 1102 26-27 0.9390681003584262 1102 28-29 1.0259856630824373 1102 30-31 4.179211469534046 1102 32-33 4.202508960573475 1102 34-35 2.8405017921146936 1102 36-37 4.905913978494631 1102 38-39 3.1998207885304666 1102 40-41 4.1917562724014275 1102 42-43 5.189068100358419 1102 44-45 2.920250896057347 1102 46-47 3.1227598566308217 1102 48-49 6.029569892473113 1102 50-51 7.148745519713259 1102 52-53 7.699820788530467 1102 54-55 7.739247311827956 1102 56-57 8.272401433691751 1102 58-59 7.293906810035843 1102 60-61 8.208781362007166 1102 62-63 7.928315412186375 1102 64-65 8.187275985663081 1102 66-67 1.0492831541218663 1102 68-69 7.717741935483875 1102 70-71 1.4775985663082416 1102 72-73 5.847670250896055 1102 74-75 5.239247311827956 1102 76-77 6.717741935483865 1102 78-79 4.921146953405021 1102 80-81 6.322580645161292 1102 82-83 7.8315412186379945 1102 84-85 5.43548387096774 1102 86-87 7.18010752688172 1102 88-89 7.914874551971327 1102 90-91 10.020609318996417 1102 92-93 8.543010752688172 1102 94-95 13.158602150537636 1102 96-97 14.890681003584227 1102 98-99 13.25 1102 100-101 14.54390681003584 1103 1 0.7956989247311839 1103 2 1.4032258064516192 1103 3 1.1810035842293907 1103 4 -0.05376344086020879 1103 5 0.9569892473118244 1103 6 1.2168458781362048 1103 7 0.9677419354838719 1103 8 1.1290322580645196 1103 9 1.6433691756272353 1103 10-11 2.7446236559139763 1103 12-13 1.2849462365591364 1103 14-15 3.2634408602150557 1103 16-17 2.1451612903225836 1103 18-19 -0.8942652329749166 1103 20-21 2.1353046594982033 1103 22-23 3.2392473118279597 1103 24-25 3.5197132616487394 1103 26-27 1.4390681003584262 1103 28-29 1.2759856630824373 1103 30-31 1.9292114695340459 1103 32-33 4.952508960573475 1103 34-35 5.340501792114694 1103 36-37 3.155913978494631 1103 38-39 1.6998207885304666 1103 40-41 0.19175627240142745 1103 42-43 5.189068100358419 1103 44-45 5.920250896057347 1103 46-47 7.622759856630822 1103 48-49 9.529569892473113 1103 50-51 8.398745519713259 1103 52-53 7.949820788530467 1103 54-55 7.489247311827956 1103 56-57 10.522401433691751 1103 58-59 11.043906810035843 1103 60-61 8.708781362007166 1103 62-63 10.678315412186375 1103 64-65 10.187275985663081 1103 66-67 11.299283154121866 1103 68-69 7.467741935483875 1103 70-71 6.977598566308242 1103 72-73 7.347670250896055 1103 74-75 7.989247311827956 1103 76-77 8.217741935483865 1103 78-79 9.421146953405021 1103 80-81 10.072580645161292 1103 82-83 10.581541218637994 1103 84-85 10.18548387096774 1103 86-87 9.43010752688172 1103 88-89 12.414874551971327 1103 90-91 13.770609318996417 1103 92-93 13.793010752688172 1103 94-95 2.408602150537636 1103 96-97 13.140681003584227 1103 98-99 15.25 1103 100-101 17.293906810035843 1104 1 1.7956989247311839 1104 2 2.903225806451619 1104 3 -1.3189964157706093 1104 4 -0.05376344086020879 1104 5 -0.04301075268817556 1104 6 1.7168458781362048 1104 7 1.9677419354838719 1104 8 2.1290322580645196 1104 9 2.6433691756272353 1104 10-11 2.2446236559139763 1104 12-13 3.2849462365591364 1104 14-15 3.5134408602150557 1104 16-17 -1.3548387096774164 1104 18-19 1.6057347670250834 1104 20-21 3.3853046594982033 1104 22-23 4.23924731182796 1104 24-25 3.0197132616487394 1104 26-27 3.4390681003584262 1104 28-29 4.275985663082437 1104 30-31 5.429211469534046 1104 32-33 6.452508960573475 1104 34-35 5.840501792114694 1104 36-37 6.405913978494631 1104 38-39 4.699820788530467 1104 40-41 7.4417562724014275 1104 42-43 7.939068100358419 1104 44-45 8.170250896057347 1104 46-47 8.372759856630822 1104 48-49 10.029569892473113 1104 50-51 10.398745519713259 1104 52-53 11.199820788530467 1104 54-55 11.239247311827956 1104 56-57 11.772401433691751 1104 58-59 12.293906810035843 1104 60-61 10.208781362007166 1104 62-63 13.178315412186375 1104 64-65 12.437275985663081 1104 66-67 13.549283154121866 1104 68-69 14.467741935483875 1104 70-71 12.977598566308242 1104 72-73 13.097670250896055 1104 74-75 12.989247311827956 1104 76-77 10.217741935483865 1104 78-79 12.671146953405021 1104 80-81 13.322580645161292 1104 82-83 11.581541218637994 1104 84-85 10.18548387096774 1104 86-87 13.68010752688172 1104 88-89 13.414874551971327 1104 90-91 12.520609318996417 1104 92-93 14.043010752688172 1104 94-95 14.658602150537636 1104 96-97 17.140681003584227 1104 98-99 18.0 1104 100-101 19.043906810035843 1105 1 -0.20430107526881613 1105 2 -1.0967741935483808 1105 3 -0.31899641577060933 1105 4 -0.05376344086020879 1105 5 -0.04301075268817556 1105 6 -0.2831541218637952 1105 7 -3.032258064516128 1105 8 -5.87096774193548 1105 9 -4.356630824372765 1105 10-11 -6.255376344086024 1105 12-13 0.2849462365591364 1105 14-15 0.5134408602150557 1105 16-17 1.1451612903225836 1105 18-19 2.1057347670250834 1105 20-21 -2.6146953405017967 1105 22-23 1.7392473118279597 1105 24-25 -10.48028673835126 1105 26-27 -1.0609318996415738 1105 28-29 -0.7240143369175627 1105 30-31 -2.070788530465954 1105 32-33 -3.547491039426525 1105 34-35 1.8405017921146936 1105 36-37 3.905913978494631 1105 38-39 0.1998207885304666 1105 40-41 0.44175627240142745 1105 42-43 -10.06093189964158 1105 44-45 -2.829749103942653 1105 46-47 -2.6272401433691783 1105 48-49 8.529569892473113 1105 50-51 3.898745519713259 1105 52-53 8.699820788530467 1105 54-55 10.239247311827956 1105 56-57 10.772401433691751 1105 58-59 8.793906810035843 1105 60-61 8.708781362007166 1105 62-63 8.678315412186375 1105 64-65 4.4372759856630815 1105 66-67 12.549283154121866 1105 68-69 11.467741935483875 1105 70-71 12.477598566308242 1105 72-73 8.597670250896055 1105 74-75 3.989247311827956 1105 76-77 4.217741935483865 1105 78-79 3.671146953405021 1105 80-81 2.322580645161292 1105 82-83 6.5815412186379945 1105 84-85 10.68548387096774 1105 86-87 11.18010752688172 1105 88-89 9.414874551971327 1105 90-91 13.520609318996417 1105 92-93 9.543010752688172 1105 94-95 9.658602150537636 1105 96-97 -0.35931899641577303 1105 98-99 5.0 1105 100-101 10.04390681003584 1106 1 1.7956989247311839 1106 2 1.9032258064516192 1106 3 2.6810035842293907 1106 4 -2.053763440860209 1106 5 -0.04301075268817556 1106 6 -0.2831541218637952 1106 7 -0.032258064516128115 1106 8 2.1290322580645196 1106 9 2.6433691756272353 1106 10-11 2.2446236559139763 1106 12-13 2.2849462365591364 1106 14-15 4.013440860215056 1106 16-17 3.1451612903225836 1106 18-19 3.6057347670250834 1106 20-21 3.8853046594982033 1106 22-23 4.23924731182796 1106 24-25 4.019713261648739 1106 26-27 3.9390681003584262 1106 28-29 2.2759856630824373 1106 30-31 4.429211469534046 1106 32-33 4.952508960573475 1106 34-35 3.8405017921146936 1106 36-37 2.905913978494631 1106 38-39 5.699820788530467 1106 40-41 7.4417562724014275 1106 42-43 8.43906810035842 1106 44-45 8.170250896057347 1106 46-47 7.872759856630822 1106 48-49 10.029569892473113 1106 50-51 2.398745519713259 1106 52-53 7.699820788530467 1106 54-55 9.239247311827956 1106 56-57 5.272401433691751 1106 58-59 8.293906810035843 1106 60-61 4.708781362007166 1106 62-63 8.678315412186375 1106 64-65 7.4372759856630815 1106 66-67 8.049283154121866 1106 68-69 2.4677419354838754 1106 70-71 5.477598566308242 1106 72-73 8.597670250896055 1106 74-75 4.489247311827956 1106 76-77 7.717741935483865 1106 78-79 10.671146953405021 1106 80-81 11.322580645161292 1106 82-83 8.581541218637994 1106 84-85 11.18548387096774 1106 86-87 12.18010752688172 1106 88-89 12.914874551971327 1106 90-91 14.520609318996417 1106 92-93 14.543010752688172 1106 94-95 14.158602150537636 1106 96-97 16.140681003584227 1106 98-99 17.5 1106 100-101 15.04390681003584 1107 1 1.2956989247311839 1107 2 1.4032258064516192 1107 3 1.1810035842293907 1107 4 0.9462365591397912 1107 5 0.9569892473118244 1107 6 0.7168458781362048 1107 7 -1.5322580645161281 1107 8 -1.3709677419354804 1107 9 -2.3566308243727647 1107 10-11 1.7446236559139763 1107 12-13 -3.4650537634408636 1107 14-15 -3.9865591397849443 1107 16-17 -2.8548387096774164 1107 18-19 -2.6442652329749166 1107 20-21 -2.3646953405017967 1107 22-23 2.2392473118279597 1107 24-25 0.019713261648739433 1107 26-27 -1.0609318996415738 1107 28-29 0.025985663082437327 1107 30-31 0.4292114695340459 1107 32-33 -3.297491039426525 1107 34-35 -4.159498207885306 1107 36-37 -1.344086021505369 1107 38-39 -1.8001792114695334 1107 40-41 -1.0582437275985725 1107 42-43 -0.060931899641580856 1107 44-45 -0.5797491039426532 1107 46-47 -5.877240143369178 1107 48-49 -14.220430107526887 1107 50-51 -14.351254480286741 1107 52-53 -12.550179211469533 1107 54-55 -10.760752688172044 1107 56-57 -8.227598566308249 1107 58-59 -7.206093189964157 1107 60-61 -12.541218637992834 1107 62-63 -10.071684587813625 1107 64-65 -8.562724014336919 1107 66-67 -10.450716845878134 1107 68-69 -12.532258064516125 1107 70-71 -9.272401433691758 1107 72-73 -9.902329749103945 1107 74-75 -10.260752688172044 1107 76-77 -8.032258064516135 1107 78-79 -8.328853046594979 1107 80-81 -6.677419354838708 1107 82-83 -6.9184587813620055 1107 84-85 -7.81451612903226 1107 86-87 -5.06989247311828 1107 88-89 -5.585125448028673 1107 90-91 -2.979390681003583 1107 92-93 -3.456989247311828 1107 94-95 -3.341397849462364 1107 96-97 -4.359318996415773 1107 98-99 -1.5 1107 100-101 0.5439068100358408 1108 1 -0.20430107526881613 1108 2 -0.0967741935483808 1108 3 -1.8189964157706093 1108 4 -4.553763440860209 1108 5 -4.543010752688176 1108 6 0.7168458781362048 1108 7 0.9677419354838719 1108 8 1.1290322580645196 1108 9 2.6433691756272353 1108 10-11 2.2446236559139763 1108 12-13 2.2849462365591364 1108 14-15 3.0134408602150557 1108 16-17 1.3951612903225836 1108 18-19 0.6057347670250834 1108 20-21 -1.6146953405017967 1108 22-23 -5.01075268817204 1108 24-25 1.0197132616487394 1108 26-27 1.1890681003584262 1108 28-29 3.0259856630824373 1108 30-31 3.929211469534046 1108 32-33 -1.547491039426525 1108 34-35 1.3405017921146936 1108 36-37 -5.844086021505369 1108 38-39 -16.550179211469533 1108 40-41 -12.808243727598573 1108 42-43 -12.06093189964158 1108 44-45 -12.329749103942653 1108 46-47 -11.377240143369178 1108 48-49 -9.470430107526887 1108 50-51 -9.601254480286741 1108 52-53 -9.300179211469533 1108 54-55 -12.010752688172044 1108 56-57 -8.727598566308249 1108 58-59 -7.706093189964157 1108 60-61 -8.291218637992834 1108 62-63 -8.071684587813625 1108 64-65 -7.3127240143369185 1108 66-67 -5.950716845878134 1108 68-69 -6.532258064516125 1108 70-71 -6.522401433691758 1108 72-73 -4.902329749103945 1108 74-75 -5.260752688172044 1108 76-77 -5.782258064516135 1108 78-79 -5.328853046594979 1108 80-81 -4.677419354838708 1108 82-83 -5.4184587813620055 1108 84-85 -5.31451612903226 1108 86-87 -4.31989247311828 1108 88-89 -3.0851254480286734 1108 90-91 -2.229390681003583 1108 92-93 -1.956989247311828 1108 94-95 -1.8413978494623642 1108 96-97 0.14068100358422697 1108 98-99 1.25 1108 100-101 -0.45609318996415915 1109 1 1.2956989247311839 1109 2 -0.5967741935483808 1109 3 -1.3189964157706093 1109 4 -0.05376344086020879 1109 5 -2.5430107526881756 1109 6 0.7168458781362048 1109 7 -2.532258064516128 1109 8 -0.8709677419354804 1109 9 0.6433691756272353 1109 10-11 1.7446236559139763 1109 12-13 -0.7150537634408636 1109 14-15 -5.486559139784944 1109 16-17 1.3951612903225836 1109 18-19 2.1057347670250834 1109 20-21 3.3853046594982033 1109 22-23 1.2392473118279597 1109 24-25 2.2697132616487394 1109 26-27 -1.3109318996415738 1109 28-29 0.025985663082437327 1109 30-31 1.4292114695340459 1109 32-33 1.202508960573475 1109 34-35 3.8405017921146936 1109 36-37 0.9059139784946311 1109 38-39 3.1998207885304666 1109 40-41 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30-31 0.9292114695340459 2316 32-33 1.452508960573475 2316 34-35 -2.6594982078853064 2316 36-37 -7.094086021505369 2316 38-39 -7.133512544802866 2316 40-41 -7.724910394265237 2316 42-43 -8.06093189964158 2316 44-45 -9.496415770609321 2316 46-47 -16.62724014336918 2316 48-49 -15.470430107526887 2316 50-51 -15.601254480286741 2316 52-53 -14.800179211469533 2316 54-55 -14.760752688172044 2316 56-57 -14.394265232974917 2316 58-59 -13.372759856630825 2316 60-61 -14.291218637992834 2316 62-63 -13.321684587813625 2316 64-65 -13.229390681003585 2316 66-67 -13.284050179211468 2316 68-69 -12.865591397849458 2316 70-71 -13.522401433691758 2316 72-73 -12.902329749103945 2316 74-75 -12.010752688172044 2316 76-77 -10.782258064516135 2316 78-79 -11.662186379928311 2316 80-81 -11.177419354838708 2316 82-83 -11.251792114695338 2316 84-85 -11.147849462365592 2316 86-87 -9.31989247311828 2316 88-89 -8.585125448028673 2316 90-91 -7.479390681003583 2316 92-93 -6.956989247311828 2316 94-95 -6.341397849462364 2316 96-97 -5.192652329749105 2316 98-99 -4.0 2316 100-101 -2.6227598566308252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 3.0 9 2.0 10 5.0 11 14.0 12 20.0 13 22.0 14 20.0 15 27.0 16 38.0 17 48.0 18 42.0 19 25.0 20 32.0 21 37.0 22 31.0 23 36.0 24 40.0 25 51.0 26 56.0 27 36.0 28 51.0 29 59.0 30 71.0 31 73.0 32 79.0 33 117.0 34 138.0 35 146.0 36 156.0 37 148.0 38 63.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73372781065088 19.053254437869825 14.615384615384617 21.597633136094675 2 16.49911295091662 21.052631578947366 39.739798935541096 22.708456534594916 3 16.558249556475456 27.38024837374335 31.40153755174453 24.659964518036663 4 12.18214074512123 19.101123595505616 36.72383205204021 31.99290360733294 5 11.64991129509166 37.67001774098166 33.944411590774685 16.735659373151982 6 27.61679479597871 39.739798935541096 16.6173861620343 16.02602010644589 7 26.7297457125961 36.07332939089296 19.988172678888233 17.20875221762271 8 24.068598462448257 35.36369012418687 18.332347723240687 22.23536369012419 9 25.547013601419277 17.327025428740388 20.69781194559432 36.42814902424601 10-11 23.2702542874039 28.562980484920164 24.807806031933765 23.358959195742166 12-13 26.552335895919576 26.84801892371378 26.7297457125961 19.869899467770548 14-15 22.087522176227083 27.35068007096393 27.942046126552334 22.619751626256654 16-17 18.628030751034892 30.691898285038437 29.065641632170312 21.61442933175636 18-19 18.894145476049676 26.40449438202247 34.29923122412774 20.40212891780012 20-21 18.83500887049083 26.138379657007686 35.8367829686576 19.189828503843877 22-23 19.544648137196923 25.901833234772326 35.06800709639267 19.485511531638082 24-25 19.160260201064457 24.275576581904197 35.89591957421644 20.6682436428149 26-27 18.421052631578945 25.251330573625076 36.69426374926079 19.633353045535188 28-29 18.48018923713779 26.907155529272618 35.8367829686576 18.77587226493199 30-31 18.71673565937315 25.901833234772326 35.45239503252514 19.92903607332939 32-33 18.56889414547605 26.43406268480189 35.00887049083383 19.988172678888233 34-35 19.89946777054997 27.261975162625667 34.062684801892374 18.77587226493199 36-37 18.74630396215257 27.61679479597871 34.41750443524542 19.2193968066233 38-39 19.278533412182142 27.557658190419872 33.678296865759904 19.485511531638082 40-41 18.657599053814312 26.43406268480189 35.71850975753992 19.189828503843877 42-43 18.657599053814312 27.823772915434652 33.944411590774685 19.574216439976343 44-45 20.047309284447074 27.67593140153755 33.20520402128918 19.0715552927262 46-47 18.657599053814312 28.237729154346543 32.58426966292135 20.5204021289178 48-49 19.101123595505616 28.592548787699585 32.46599645180367 19.840331164991127 50-51 18.71673565937315 28.976936723832054 31.99290360733294 20.313424009461855 52-53 19.012418687167358 30.011827321111767 32.0816085156712 18.894145476049676 54-55 18.657599053814312 29.450029568302778 31.16499112950917 20.727380248373745 56-57 19.89946777054997 27.971614429331755 30.83973979893554 21.289178001182734 58-59 20.5204021289178 28.1785925487877 31.25369603784743 20.047309284447074 60-61 20.017740981667654 29.331756357185096 31.076286221170907 19.574216439976343 62-63 19.278533412182142 28.00118273211118 31.57894736842105 21.14133648728563 64-65 18.894145476049676 29.834417504435244 31.046717918391487 20.224719101123593 66-67 19.04198698994678 28.0307510348906 31.40153755174453 21.525724423418097 68-69 18.982850384387937 27.76463630987581 31.726788882318157 21.525724423418097 70-71 19.72205795387345 28.14902424600828 32.377291543465404 19.75162625665287 72-73 19.840331164991127 27.38024837374335 32.52513305736251 20.254287403903014 74-75 19.337670017740983 28.11945594322886 30.573625073920756 21.969248965109404 76-77 18.566775244299674 28.36837429671306 30.91501332543678 22.149837133550488 78-79 18.48018923713779 29.124778237729153 31.076286221170907 21.31874630396215 80-81 20.047309284447074 28.474275576581903 30.86930810171496 20.60910703725606 82-83 19.544648137196923 28.08988764044944 30.86930810171496 21.496156120638677 84-85 19.160260201064457 28.1785925487877 30.987581312832646 21.673565937315196 86-87 18.31360946745562 28.994082840236686 30.680473372781066 22.011834319526628 88-89 19.82809721398933 28.71962062833432 28.867812685240068 22.58446947243628 90-91 18.068720379146917 27.75473933649289 30.953791469194314 23.22274881516588 92-93 18.953282081608517 28.592548787699585 30.337078651685395 22.117090479006503 94-95 19.69248965109403 27.76463630987581 29.775280898876407 22.767593140153757 96-97 18.391484328799525 28.296865759905383 29.893554109994085 23.418095801301007 98-99 19.2193968066233 27.49852158486103 29.775280898876407 23.50680070963927 100-101 19.426374926079244 27.70549970431697 29.775280898876407 23.092844470727382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 8.0 2 5.5 3 7.0 4 4.5 5 2.5 6 6.5 7 12.0 8 17.5 9 23.0 10 18.0 11 10.0 12 8.5 13 5.0 14 5.5 15 7.0 16 5.5 17 6.5 18 9.0 19 8.0 20 7.0 21 8.5 22 9.5 23 7.5 24 12.0 25 15.0 26 17.0 27 20.0 28 17.5 29 18.5 30 18.5 31 21.5 32 23.5 33 27.5 34 38.0 35 37.0 36 43.0 37 46.5 38 53.0 39 56.0 40 39.0 41 38.5 42 45.0 43 48.5 44 51.5 45 48.0 46 52.0 47 51.5 48 51.0 49 58.5 50 51.0 51 44.5 52 43.5 53 43.5 54 43.5 55 42.0 56 34.0 57 35.5 58 40.5 59 36.0 60 32.0 61 21.5 62 14.5 63 13.5 64 10.0 65 6.0 66 4.0 67 4.5 68 5.0 69 5.5 70 4.0 71 0.5 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05913660555884093 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.14784151389710232 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.05913660555884093 88-89 0.2365464222353637 90-91 0.17740981667652278 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1691.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.51685393258427 #Duplication Level Percentage of deduplicated Percentage of total 1 95.21531100478468 82.3772915434654 2 2.6657552973342447 4.612655233589591 3 0.888585099111415 2.3063276167947957 4 0.4101161995898838 1.4192785334121822 5 0.1367053998632946 0.5913660555884093 6 0.0683526999316473 0.35481963335304556 7 0.0683526999316473 0.4139562389118865 8 0.1367053998632946 0.9461856889414548 9 0.0683526999316473 0.5322294500295683 >10 0.3417634996582365 6.445890005913661 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38 2.247191011235955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 1.655824955647546 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 1.0053222945002958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.9461856889414548 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.5913660555884093 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 9 0.5322294500295683 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 8 0.4730928444707274 No Hit ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA 8 0.4730928444707274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7 0.4139562389118865 No Hit CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA 6 0.35481963335304556 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 5 0.29568302779420463 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.29568302779420463 No Hit GTGTGGACTCGGCCCAGTCTTTCTGAGGCAGGCAGGCAGGGGCCACGTTC 4 0.2365464222353637 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2365464222353637 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2365464222353637 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 4 0.2365464222353637 No Hit GTCGTAGCGCACGTACTCCTCCCGGTTGTAGATGTATCTGGTCACATCTC 4 0.2365464222353637 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2365464222353637 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT 3 0.17740981667652278 No Hit GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT 3 0.17740981667652278 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3 0.17740981667652278 No Hit GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC 3 0.17740981667652278 No Hit ACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTT 3 0.17740981667652278 No Hit GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA 3 0.17740981667652278 No Hit TTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT 3 0.17740981667652278 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3 0.17740981667652278 No Hit GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA 3 0.17740981667652278 No Hit GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA 3 0.17740981667652278 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3 0.17740981667652278 No Hit CTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATG 3 0.17740981667652278 No Hit GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT 3 0.17740981667652278 No Hit ACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTT 2 0.11827321111768185 No Hit AGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA 2 0.11827321111768185 TruSeq Adapter, Index 3 (96% over 28bp) GTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTAT 2 0.11827321111768185 No Hit TAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGAT 2 0.11827321111768185 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2 0.11827321111768185 No Hit GTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11827321111768185 No Hit GTCTTTCATCTTTCCCTTGCGGTACTAGTTCTATAGCTCCTAGATGTACG 2 0.11827321111768185 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11827321111768185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 2 0.11827321111768185 No Hit CCTAATAAGCTGTGTGGATGAGACCCCCACCGTCTCCTCCTGGCCATTCC 2 0.11827321111768185 No Hit GTCGTAGGTGTCCCTCTGAAACTTGTTGTGCTTAATGACCACGTCCACCT 2 0.11827321111768185 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2 0.11827321111768185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC 2 0.11827321111768185 No Hit CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA 2 0.11827321111768185 No Hit GTTCAAGCCGCCGCAGGGAGGTGTGGGTCTCCGGCCCCTCGTAGTTGTGT 2 0.11827321111768185 No Hit GATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAGA 2 0.11827321111768185 No Hit CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT 2 0.11827321111768185 No Hit CTCCGGCCCCTCGTAGTTGTGTCTGCACACCGTGTCCAGCTCGGCCCGCG 2 0.11827321111768185 No Hit CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA 2 0.11827321111768185 No Hit ACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTA 2 0.11827321111768185 No Hit TTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC 2 0.11827321111768185 No Hit TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATA 2 0.11827321111768185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2 0.11827321111768185 No Hit CCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCT 2 0.11827321111768185 No Hit GCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACG 2 0.11827321111768185 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 2 0.11827321111768185 No Hit TCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGAC 2 0.11827321111768185 No Hit ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA 2 0.11827321111768185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 2 0.11827321111768185 No Hit GTACATGGGAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11827321111768185 No Hit GTTCTATAATCGATAAACCCCGCTCTACCTCACCATCTCTTGCTAATTCA 2 0.11827321111768185 No Hit GCTATGAAGTTATCAGGGAGTAACTGCCCATTTGAAAGAAACTCGTCATC 2 0.11827321111768185 No Hit GGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGAGGTGT 2 0.11827321111768185 No Hit CTGTAGGATCAGCTTGGTATTTCAAGGGATCCTCCTTGCCACGGTGGTCA 2 0.11827321111768185 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2 0.11827321111768185 No Hit GACCAGGACCTGGAAGGTCCAGTCCCCATTCCTAATAAGCTGTGTGGATG 2 0.11827321111768185 No Hit ATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTC 2 0.11827321111768185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 2 0.11827321111768185 No Hit ATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCA 2 0.11827321111768185 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.02956830277942046 0.0 28-29 0.0 0.0 0.0 0.05913660555884092 0.0 30-31 0.0 0.0 0.0 0.05913660555884092 0.0 32-33 0.0 0.0 0.0 0.05913660555884092 0.0 34-35 0.0 0.0 0.0 0.05913660555884092 0.0 36-37 0.0 0.0 0.0 0.05913660555884092 0.0 38-39 0.0 0.0 0.0 0.05913660555884092 0.0 40-41 0.0 0.0 0.0 0.05913660555884092 0.0 42-43 0.0 0.0 0.0 0.11827321111768184 0.0 44-45 0.0 0.0 0.0 0.17740981667652278 0.0 46-47 0.0 0.0 0.0 0.17740981667652278 0.0 48-49 0.0 0.0 0.0 0.20697811945594324 0.0 50-51 0.0 0.0 0.0 0.23654642223536368 0.0 52-53 0.0 0.0 0.0 0.29568302779420463 0.0 54-55 0.0 0.0 0.0 0.384387936132466 0.0 56-57 0.0 0.0 0.0 0.4435245416913069 0.0 58-59 0.0 0.0 0.0 0.5026611472501479 0.0 60-61 0.0 0.0 0.0 0.5322294500295683 0.0 62-63 0.0 0.0 0.0 0.5322294500295683 0.0 64-65 0.0 0.0 0.0 0.6800709639266707 0.0 66-67 0.0 0.0 0.0 0.7392075694855116 0.0 68-69 0.0 0.0 0.0 0.768775872264932 0.0 70-71 0.0 0.0 0.0 0.7983441750443525 0.0 72-73 0.0 0.0 0.0 1.0644589000591367 0.0 74-75 0.0 0.0 0.0 1.0940272028385571 0.0 76-77 0.0 0.0 0.0 1.153163808397398 0.0 78-79 0.0 0.0 0.0 1.2418687167356595 0.0 80-81 0.0 0.0 0.0 1.6262566528681253 0.0 82-83 0.0 0.0 0.0 1.803666469544648 0.0 84-85 0.0 0.0 0.0 2.1880544056771143 0.0 86-87 0.0 0.0 0.0 2.3358959195742166 0.0 88-89 0.0 0.0 0.0 2.5133057362507394 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20 1.2763348E-5 95.00001 1 AACGCAG 30 9.54892E-5 63.333336 7 GTATCAA 30 9.54892E-5 63.333336 2 TCAACGC 30 9.54892E-5 63.333336 5 TATCAAC 30 9.54892E-5 63.333336 3 ATCAACG 30 9.54892E-5 63.333336 4 CAACGCA 30 9.54892E-5 63.333336 6 ACGCAGA 35 2.0485882E-4 54.28571 8 CGCAGAG 35 2.0485882E-4 54.28571 9 CAGAGTA 35 3.1462878E-6 40.714283 10-11 GAGTACT 25 0.0013934055 38.0 12-13 GTACTTT 30 0.0034115312 31.666668 14-15 AGAGTAC 35 0.007255217 27.142855 12-13 GCAGAGT 35 0.007255217 27.142855 9 >>END_MODULE