FastQCFastQC Report
Fri 27 May 2016
SRR939230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281.6857314870559903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271.6255267910897049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181.0836845273931366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171.0234798314268514No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC80.481637567730283No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT70.4214328717639976No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA70.4214328717639976No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3612281757977122No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3612281757977122No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3612281757977122No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA50.30102347983142685No Hit
TCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTAT50.30102347983142685No Hit
GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA50.30102347983142685No Hit
GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT50.30102347983142685No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40.2408187838651415No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2408187838651415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2408187838651415No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2408187838651415No Hit
TTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT40.2408187838651415No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT40.2408187838651415No Hit
ACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTT30.1806140878988561No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30.1806140878988561No Hit
CTCTGTGAGGGACCGACTGCGCGCATTCAATGATTATTCTTTAGTTGTTG30.1806140878988561No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG30.1806140878988561RNA PCR Primer, Index 26 (100% over 29bp)
CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT30.1806140878988561No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.1806140878988561No Hit
TTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC30.1806140878988561No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT30.1806140878988561No Hit
CCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCT30.1806140878988561No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA30.1806140878988561RNA PCR Primer, Index 31 (96% over 29bp)
GACCAGAGTGTTGTGGTGGTTGAGGGCCTCTGTCCTGGACAGGGAGATGA30.1806140878988561No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA30.1806140878988561RNA PCR Primer, Index 1 (96% over 28bp)
GTCGTAGCGCACGTACTCCTCCCGGTTGTAGATGTATCTGGTCACATCTC30.1806140878988561No Hit
CTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATG30.1806140878988561No Hit
GAACATATTCTGTGTGATGCTGAAGCTGGTGGAGAGCTTGCAGGTGTTGC20.12040939193257075No Hit
CACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAG20.12040939193257075No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12040939193257075No Hit
ATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAAT20.12040939193257075No Hit
GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT20.12040939193257075No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA20.12040939193257075No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG20.12040939193257075RNA PCR Primer, Index 26 (96% over 29bp)
CATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAG20.12040939193257075No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12040939193257075No Hit
GGTGTGGGTCTCCGGCCCCTCGTAGTTGTGTCTGCACACCGTGTCCAGCT20.12040939193257075No Hit
GTGTGGACTCGGCCCAGTCTTTCTGAGGCAGGCAGGCAGGGGCCACGTTC20.12040939193257075No Hit
GTTCAAGCCGCCGCAGGGAGGTGTGGGTCTCCGGCCCCTCGTAGTTGTGT20.12040939193257075No Hit
GTGTAGACCTCTCCCCGCCGAGGGGTCATCTCCAGCATGACCAGGACCTG20.12040939193257075No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC20.12040939193257075No Hit
TTTCTTCTCTGTGCAGGCAGCTATGAAGTTATCAGGGAGTAACTGCCCAT20.12040939193257075No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT20.12040939193257075No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG20.12040939193257075No Hit
GCTGTGTGGATGAGACCCCCACCGTCTCCTCCTGGCCATTCCGGAACCAG20.12040939193257075No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20.12040939193257075No Hit
GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA20.12040939193257075No Hit
AGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCC20.12040939193257075No Hit
ATCCTCTTCAGCCTTTTCTCGTTTTCCGAAGGCTCCACCAGCTTCTCGGA20.12040939193257075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC20.12040939193257075No Hit
TCCTATAACAGAGCAACTCTGCCTGATGGGGCCTCTGATTCTTGCATCAA20.12040939193257075No Hit
GTAGTTGTGTCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCAGGA20.12040939193257075No Hit
TTCCAGTACTCGGCGTCTGGCCGCCCCAGCTCGGTCACCGCGCGGTGCTC20.12040939193257075No Hit
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA20.12040939193257075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG300.00860457547.56
GTATCAA300.00860457547.51
TCAACGC300.00860457547.54
TATCAAC300.00860457547.52
ATCAACG300.00860457547.53
CAACGCA300.00860457547.55
GTACTTT306.630262E-539.58333614-15
ACTTTTT306.630262E-539.58333616-17
CAGAGTA351.6447133E-433.9285710-11
GAGTACT351.6447133E-433.9285712-13
Produced by FastQC (version 0.11.5)