FastQCFastQC Report
Fri 27 May 2016
SRR939229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432882
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84880.24725580430670205No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73570.2143097257639499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68140.19849211245827847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.12359877211043083No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40730.11864666481399594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115150.025.2720581
TACATGG117500.024.1094882
ACATGGG120450.022.7717513
GTATCAA183750.021.960171
GAGTACT91300.021.73281112-13
GTGTAGC19100.020.6295051
CATGGGG77450.020.4902884
GGTATCA143200.020.4212271
GTACTTT100250.019.60491214-15
AGAGTAC150750.019.24030510-11
ATCAACG207400.019.1636353
CAACGCA208600.019.0988125
TCAACGC208600.019.0988124
TATCAAC212050.018.832762
AACGCAG212000.018.830616
GTATAGC9100.018.2587381
AGTACTT97750.018.16716612-13
GTATAGG7850.018.142441
TAGCCCT20550.017.519694
ACGCAGA232700.017.094477
Produced by FastQC (version 0.11.5)