##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3432882 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99759356715436 33.0 31.0 34.0 30.0 34.0 2 32.10619328016518 34.0 31.0 34.0 30.0 34.0 3 32.16977163794153 34.0 31.0 34.0 30.0 34.0 4 35.604629579461225 37.0 35.0 37.0 33.0 37.0 5 35.64405039264385 37.0 35.0 37.0 35.0 37.0 6 35.72488742694914 37.0 36.0 37.0 35.0 37.0 7 35.6441785648327 37.0 35.0 37.0 33.0 37.0 8 35.632300207231125 37.0 35.0 37.0 33.0 37.0 9 37.29947228014246 39.0 38.0 39.0 34.0 39.0 10-11 37.202883320778284 39.0 37.0 39.0 34.0 39.0 12-13 37.10314569507487 39.0 37.0 39.0 34.0 39.0 14-15 38.40049963849617 40.0 38.0 41.0 34.0 41.0 16-17 38.330690073238756 40.0 38.0 41.0 33.5 41.0 18-19 38.23578162605065 40.0 38.0 41.0 33.5 41.0 20-21 38.12049540298793 40.0 38.0 41.0 33.0 41.0 22-23 38.03435043791194 40.0 38.0 41.0 33.0 41.0 24-25 38.07863334655838 40.0 38.0 41.0 33.0 41.0 26-27 37.99151325329563 40.0 38.0 41.0 33.0 41.0 28-29 37.86240336836512 40.0 38.0 41.0 33.0 41.0 30-31 37.699566865391816 40.0 38.0 41.0 32.5 41.0 32-33 37.498351093920505 40.0 37.5 41.0 32.0 41.0 34-35 37.1822893999852 40.0 37.0 41.0 31.0 41.0 36-37 36.923899656323755 40.0 37.0 41.0 30.5 41.0 38-39 36.67540567954273 40.0 36.5 41.0 30.0 41.0 40-41 36.52519661322469 40.0 36.0 41.0 30.0 41.0 42-43 36.326575745976704 39.5 36.0 41.0 30.0 41.0 44-45 36.07564489545519 39.0 35.0 41.0 29.0 41.0 46-47 35.731142666715606 39.0 35.0 41.0 27.5 41.0 48-49 35.73124068931003 39.0 35.0 41.0 27.0 41.0 50-51 34.74448626547606 38.0 34.0 40.0 25.5 40.5 52-53 34.91726412384696 38.5 34.5 39.5 26.0 40.5 54-55 35.40750803552234 39.0 35.0 41.0 27.0 41.0 56-57 35.278789367068256 39.0 35.0 41.0 26.0 41.0 58-59 35.076927491244966 39.0 35.0 41.0 26.0 41.0 60-61 34.764344361384985 38.0 34.5 40.0 26.0 41.0 62-63 34.38471552473986 38.0 34.0 40.0 24.5 41.0 64-65 34.00567074545528 37.0 34.0 40.0 23.0 41.0 66-67 33.60309151319504 36.5 34.0 39.5 22.5 41.0 68-69 33.186076305564825 36.0 33.0 39.0 22.0 41.0 70-71 32.777854583990944 35.5 33.0 39.0 20.5 40.5 72-73 32.32271251968463 35.0 32.5 38.0 19.5 40.0 74-75 31.867277698446962 35.0 32.0 37.0 18.0 39.5 76-77 31.432589439427282 35.0 31.5 37.0 15.5 39.0 78-79 31.010557892755997 35.0 31.0 36.0 11.0 39.0 80-81 30.58190304822595 35.0 31.0 36.0 7.0 37.5 82-83 30.160829297365886 34.5 30.5 35.0 4.5 37.0 84-85 29.785002513922706 34.0 30.0 35.0 2.0 36.5 86-87 29.40293257968086 34.0 30.0 35.0 2.0 36.0 88-89 28.998754690665162 34.0 29.0 35.0 2.0 36.0 90-91 28.610472629120373 34.0 29.0 35.0 2.0 35.5 92-93 28.185044665094807 34.0 29.0 35.0 2.0 35.0 94-95 27.71672256721903 34.0 27.5 35.0 2.0 35.0 96-97 27.129492653694477 34.0 26.5 35.0 2.0 35.0 98-99 26.399724488054062 33.5 25.0 35.0 2.0 35.0 100-101 23.80072909584425 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.059784898894108096 1101 2 0.15505079711245173 1101 3 0.18258088570937048 1101 4 0.07864734400556017 1101 5 0.20422064267445705 1101 6 0.22359349834542286 1101 7 0.08522265535495421 1101 8 0.18327311971658133 1101 9 0.34245138105570305 1101 10-11 0.2818636971216506 1101 12-13 0.227752026099175 1101 14-15 0.2758502246416512 1101 16-17 0.33418570425619265 1101 18-19 0.36543989203082106 1101 20-21 0.32581953306058864 1101 22-23 0.277507781033286 1101 24-25 0.3044773457637433 1101 26-27 0.3161233094645155 1101 28-29 0.2958967549715865 1101 30-31 0.3971883426184988 1101 32-33 0.3500624732722031 1101 34-35 0.23554425470869234 1101 36-37 0.19993173475328518 1101 38-39 0.17734860850219292 1101 40-41 0.0915980943809771 1101 42-43 -0.015428380660232222 1101 44-45 -8.460037845381407E-4 1101 46-47 0.024408118055262662 1101 48-49 0.18805952831601758 1101 50-51 0.06613510949168244 1101 52-53 0.14726267753091804 1101 54-55 0.19841298410707253 1101 56-57 0.2259495793719779 1101 58-59 0.25053451358193257 1101 60-61 0.17453063814270564 1101 62-63 0.16305747789633784 1101 64-65 0.15262708563016503 1101 66-67 -0.0023746305418512748 1101 68-69 -0.013791340422017129 1101 70-71 -0.026974884603447435 1101 72-73 -0.10188133729408833 1101 74-75 -0.13245809640723039 1101 76-77 -0.1944260052444946 1101 78-79 -0.22233735974579005 1101 80-81 -0.3057427586196013 1101 82-83 -0.31044021849182357 1101 84-85 -0.45888077138473804 1101 86-87 -0.43902597669503507 1101 88-89 -0.47101835368988176 1101 90-91 -0.44077866432315815 1101 92-93 -0.5113469852713628 1101 94-95 -0.5836410932967979 1101 96-97 -0.6456987582373266 1101 98-99 -0.6883580048803744 1101 100-101 -0.7277714457122642 1102 1 0.1533630383130884 1102 2 0.2658787291034628 1102 3 0.3148384961938646 1102 4 0.34695985239167726 1102 5 0.29721562880484953 1102 6 0.27122378091298316 1102 7 0.29103852503268257 1102 8 0.34517592567626565 1102 9 0.39365539434204777 1102 10-11 0.21198004847041574 1102 12-13 0.3041906178343723 1102 14-15 0.342960828250213 1102 16-17 0.39276544559419335 1102 18-19 0.44390851221719174 1102 20-21 0.45276479672002523 1102 22-23 0.4175816062242248 1102 24-25 0.3316484115464817 1102 26-27 0.2852214427496449 1102 28-29 0.4574844894747301 1102 30-31 0.43180671621886546 1102 32-33 0.4234410984697021 1102 34-35 0.43861583173635665 1102 36-37 0.32612998386949243 1102 38-39 0.320076745045931 1102 40-41 0.31558941937337437 1102 42-43 0.2941750280043749 1102 44-45 0.3271810070345751 1102 46-47 0.39185049689130835 1102 48-49 0.33142728237078956 1102 50-51 0.22755624441708022 1102 52-53 0.3426879254176214 1102 54-55 0.3949652710176039 1102 56-57 0.45639737734946095 1102 58-59 0.3393832951650708 1102 60-61 0.2558661183213502 1102 62-63 0.24739651515669436 1102 64-65 0.324569076993491 1102 66-67 0.28945629443631304 1102 68-69 0.24932372117655888 1102 70-71 0.3129122964845976 1102 72-73 0.2726106900781957 1102 74-75 0.15634878883238557 1102 76-77 0.13668643851719509 1102 78-79 0.010993947228445933 1102 80-81 -0.012417366423274956 1102 82-83 -0.0540013529243204 1102 84-85 0.001889957036592449 1102 86-87 -0.06002480743106631 1102 88-89 0.019179818753279676 1102 90-91 -0.22858699265905358 1102 92-93 -0.24780222601103574 1102 94-95 -0.3176113605845181 1102 96-97 -0.3909250121839385 1102 98-99 -0.4488952638737125 1102 100-101 -0.4161962801121746 1103 1 0.28762556849451215 1103 2 0.3085284368064407 1103 3 0.2914137450765608 1103 4 0.25910313790407 1103 5 0.32019961374412276 1103 6 0.2658859835965899 1103 7 0.2743790028248867 1103 8 0.3301918572980611 1103 9 0.37703571422606785 1103 10-11 0.4032043584043379 1103 12-13 0.39771690370660195 1103 14-15 0.5145536775334207 1103 16-17 0.44478748390309164 1103 18-19 0.41753049211883564 1103 20-21 0.5374638689833589 1103 22-23 0.47423773872525743 1103 24-25 0.46968394610323827 1103 26-27 0.4994003775473317 1103 28-29 0.49574051141917863 1103 30-31 0.5010520110451395 1103 32-33 0.483146246130552 1103 34-35 0.44450688134602245 1103 36-37 0.39197211740307836 1103 38-39 0.45926300682516086 1103 40-41 0.3671564025865024 1103 42-43 0.4133177972383564 1103 44-45 0.3406488335024491 1103 46-47 0.26140816623323815 1103 48-49 0.27815574599923565 1103 50-51 0.3560331709438813 1103 52-53 0.44939140951467493 1103 54-55 0.4195054964070337 1103 56-57 0.3164705950155664 1103 58-59 0.35841326538780294 1103 60-61 0.4026442802659034 1103 62-63 0.24225083598983588 1103 64-65 0.11618930467749067 1103 66-67 0.0782047625495963 1103 68-69 0.03549867176506183 1103 70-71 0.12291047125788168 1103 72-73 -0.009440656156883165 1103 74-75 0.006044132418278281 1103 76-77 0.051908255712916684 1103 78-79 0.10880310152267825 1103 80-81 0.1765779766411235 1103 82-83 -0.03752889038057816 1103 84-85 0.04381395459600412 1103 86-87 0.11337929254963441 1103 88-89 0.12925924219022988 1103 90-91 -0.013176551319233454 1103 92-93 -0.16508722734084458 1103 94-95 -0.25340953222883655 1103 96-97 -0.2373955973516253 1103 98-99 -0.30255239242703524 1103 100-101 -0.3097605307001565 1104 1 0.23428470587104044 1104 2 0.337535669123767 1104 3 0.3835066044192743 1104 4 0.23313065385183762 1104 5 0.2396326422981474 1104 6 0.2661150827335348 1104 7 0.233346104066527 1104 8 0.24141624746938106 1104 9 0.4287345638182032 1104 10-11 0.3912766298061001 1104 12-13 0.3848638102669639 1104 14-15 0.46899010894340165 1104 16-17 0.41953689653082193 1104 18-19 0.4527041906410858 1104 20-21 0.47287330114158266 1104 22-23 0.4132147682755729 1104 24-25 0.4329107829881167 1104 26-27 0.5045023684473762 1104 28-29 0.5298333776919293 1104 30-31 0.4282432760086792 1104 32-33 0.3860859712482707 1104 34-35 0.33186608168404064 1104 36-37 0.4415503222482826 1104 38-39 0.4962634346535353 1104 40-41 0.31299988384980537 1104 42-43 0.3813239193216944 1104 44-45 0.46964576247335543 1104 46-47 0.33568566317433124 1104 48-49 0.38506458309078795 1104 50-51 0.40057119477630465 1104 52-53 0.5686913200559474 1104 54-55 0.5574728081997549 1104 56-57 0.47670832002037145 1104 58-59 0.5677709969639224 1104 60-61 0.6229570372763007 1104 62-63 0.7303541906819788 1104 64-65 0.5396397211222421 1104 66-67 0.5634372441600135 1104 68-69 0.4729226578332302 1104 70-71 0.46811252589394314 1104 72-73 0.4118100824534352 1104 74-75 0.4769916000269454 1104 76-77 0.5313157796628438 1104 78-79 0.4295709892429045 1104 80-81 0.38752202610696784 1104 82-83 0.4046008001134176 1104 84-85 0.42607962276547084 1104 86-87 0.39315708473103683 1104 88-89 0.5152697844193774 1104 90-91 0.470367954755055 1104 92-93 0.38322833348426144 1104 94-95 0.35186014537934795 1104 96-97 0.3881502201317808 1104 98-99 0.5130795165985944 1104 100-101 0.5703941234467713 1105 1 0.2769652505107558 1105 2 0.31054524285323737 1105 3 0.3951081396426446 1105 4 0.3136815691386232 1105 5 0.31334428288135996 1105 6 0.34247132787120904 1105 7 0.23445468834791683 1105 8 0.3062254452623705 1105 9 0.40073187827302803 1105 10-11 0.39473357193040925 1105 12-13 0.44102243474188185 1105 14-15 0.48062247414566883 1105 16-17 0.4266069727242012 1105 18-19 0.5206901214125921 1105 20-21 0.45633005400269866 1105 22-23 0.5222560206799685 1105 24-25 0.4402902547047276 1105 26-27 0.3848725373797208 1105 28-29 0.5123322097387728 1105 30-31 0.44205520616458926 1105 32-33 0.5120317144699058 1105 34-35 0.42918850548817744 1105 36-37 0.44008721811268003 1105 38-39 0.5173940730781439 1105 40-41 0.5429049633752143 1105 42-43 0.38108559711006507 1105 44-45 0.39671996261221665 1105 46-47 0.40847434682886785 1105 48-49 0.49136331213860984 1105 50-51 0.37883182613840916 1105 52-53 0.5395324853992634 1105 54-55 0.5980773845838812 1105 56-57 0.4214841576843469 1105 58-59 0.3372113656087876 1105 60-61 0.4019129618618891 1105 62-63 0.43495876507387266 1105 64-65 0.42851866539219685 1105 66-67 0.46454587824750604 1105 68-69 0.5281511034942739 1105 70-71 0.44707826228219005 1105 72-73 0.3803299346687581 1105 74-75 0.3699578822900911 1105 76-77 0.39172097224811253 1105 78-79 0.3392794940337538 1105 80-81 0.22187628497718848 1105 82-83 0.2715729774271374 1105 84-85 0.33061845309898175 1105 86-87 0.2938427155701788 1105 88-89 0.16225905518827588 1105 90-91 0.33920641662300355 1105 92-93 0.30964559110036305 1105 94-95 0.2411686174973937 1105 96-97 0.24777544911534832 1105 98-99 0.3119610707084419 1105 100-101 0.2568967188428921 1106 1 0.22600151183678463 1106 2 0.2549080668082837 1106 3 0.28642352975536767 1106 4 0.24663745516225077 1106 5 0.2829326783459152 1106 6 0.22508065899130258 1106 7 0.2967604481919679 1106 8 0.29597305762578685 1106 9 0.3081517502047859 1106 10-11 0.3772429654047542 1106 12-13 0.38695336862264895 1106 14-15 0.4605392307372327 1106 16-17 0.46950100750022017 1106 18-19 0.4635330959289732 1106 20-21 0.4400414040910121 1106 22-23 0.3996632319451052 1106 24-25 0.38903467532738034 1106 26-27 0.43384715625474257 1106 28-29 0.37382100542844654 1106 30-31 0.3967593461266361 1106 32-33 0.4039600649672721 1106 34-35 0.43852057465299765 1106 36-37 0.40019539373339796 1106 38-39 0.5771022133932675 1106 40-41 0.48635154988296136 1106 42-43 0.3595142982908115 1106 44-45 0.3464258915815108 1106 46-47 0.4476702616284669 1106 48-49 0.5056948735480589 1106 50-51 0.4103235734527644 1106 52-53 0.46968969025815 1106 54-55 0.36016424017351056 1106 56-57 0.43166996767754284 1106 58-59 0.4120690280169512 1106 60-61 0.4544465662511783 1106 62-63 0.4865724306215782 1106 64-65 0.47774896519564436 1106 66-67 0.2997319492364525 1106 68-69 0.40701628447821037 1106 70-71 0.448465193702944 1106 72-73 0.4153672380796394 1106 74-75 0.5155523214671653 1106 76-77 0.6261945722906326 1106 78-79 0.6181933151349277 1106 80-81 0.6736294646172425 1106 82-83 0.7322383552493328 1106 84-85 0.829600759485217 1106 86-87 0.6464342135624115 1106 88-89 0.8279752227836141 1106 90-91 0.6918950524883769 1106 92-93 0.9062854843483557 1106 94-95 0.7801386523379534 1106 96-97 0.9260466986775846 1106 98-99 0.8406071101691026 1106 100-101 0.7992745040234581 1107 1 0.2054714621909568 1107 2 0.16659180244025862 1107 3 0.20874652785452952 1107 4 0.2349976185608682 1107 5 0.24039063436084973 1107 6 0.2547624787811884 1107 7 0.25713718499795135 1107 8 0.2005835653639565 1107 9 0.24975212496990906 1107 10-11 0.328746723196339 1107 12-13 0.33120969385270627 1107 14-15 0.47748151001456307 1107 16-17 0.47471964458947724 1107 18-19 0.3817056484943535 1107 20-21 0.323148344112127 1107 22-23 0.38180768549312205 1107 24-25 0.5111895711515331 1107 26-27 0.3765801114579972 1107 28-29 0.281051858762126 1107 30-31 0.33782873997169816 1107 32-33 0.38119295637278583 1107 34-35 0.36723498785882924 1107 36-37 0.31380088286745433 1107 38-39 0.45901868664318357 1107 40-41 0.40081723993034046 1107 42-43 0.31011833967539815 1107 44-45 0.508998589403852 1107 46-47 0.6562872402674529 1107 48-49 0.6924438233863057 1107 50-51 0.6553516467327682 1107 52-53 0.6735518045580591 1107 54-55 0.7115745704239131 1107 56-57 0.654022240891571 1107 58-59 0.630428190113868 1107 60-61 0.5695310534687508 1107 62-63 0.6269208235869286 1107 64-65 0.65248761499533 1107 66-67 0.5329661255093825 1107 68-69 0.6714146344858065 1107 70-71 0.6571449696914726 1107 72-73 0.4712996686772044 1107 74-75 0.5738456322898564 1107 76-77 0.5142532265611628 1107 78-79 0.64333071673612 1107 80-81 0.595321669933746 1107 82-83 0.6389208117434073 1107 84-85 0.6099092113354736 1107 86-87 0.5814779266109902 1107 88-89 0.5695184296791886 1107 90-91 0.5215058353344091 1107 92-93 0.480885507066354 1107 94-95 0.39214888871518383 1107 96-97 0.5569048251411708 1107 98-99 0.5420053947874273 1107 100-101 0.5677128016941744 1108 1 0.0033332376592127844 1108 2 0.08066390400147583 1108 3 0.09087095865371708 1108 4 0.06799795672289832 1108 5 0.11408800678130149 1108 6 0.1723379781759533 1108 7 0.2181435217054215 1108 8 0.21704179380113686 1108 9 0.27595843516604646 1108 10-11 0.19357959133416358 1108 12-13 0.25142118231084254 1108 14-15 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-1.5457641743931951 2316 96-97 -1.6435200676746682 2316 98-99 -1.5332383812482178 2316 100-101 -1.3776299385732393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13170.0 3 3728.0 4 3736.0 5 4239.0 6 4828.0 7 5493.0 8 6589.0 9 7937.0 10 9442.0 11 11416.0 12 14367.0 13 20663.0 14 18110.0 15 18015.0 16 20631.0 17 25674.0 18 24702.0 19 18920.0 20 19191.0 21 20264.0 22 22097.0 23 24122.0 24 27441.0 25 30414.0 26 35274.0 27 40711.0 28 47384.0 29 56964.0 30 68363.0 31 83893.0 32 104955.0 33 137330.0 34 191257.0 35 282574.0 36 456494.0 37 734293.0 38 695618.0 39 122523.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.629456031137046 18.36412996140021 14.122449552241248 23.88396445522149 2 15.39555458078952 19.552704883393126 40.737407265578085 24.31433327023927 3 20.01579477524233 23.352791003277357 28.9973525670301 27.634061654450214 4 13.302511344609258 15.742779887894057 36.29735402793053 34.65735473956615 5 14.455011280215487 36.653057676409794 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22.790593347603338 26.186109657162582 28.27060758746746 22.752689407766617 28-29 22.61100944938753 26.654371982353055 28.111738322140173 22.62288024611924 30-31 22.865527877129253 26.11566261405977 28.04874385517938 22.970065653631597 32-33 22.61198802381589 26.255234914653503 28.166983024018567 22.96579403751204 34-35 23.228713863553267 26.23288365871102 27.92462160723727 22.61378087049844 36-37 23.080805205195418 26.09251595813116 28.06826366780214 22.758415168871284 38-39 22.829968190865344 26.423794602368254 28.073017314014308 22.67321989275209 40-41 23.043339978154894 26.34278208818272 27.89768788883983 22.716190044822554 42-43 22.780932728929084 26.42440739480549 27.62693769733689 23.167722178928535 44-45 22.97635300082076 26.328427682087103 27.663602366514727 23.0316169505774 46-47 23.172597711109127 26.586964826488956 27.31487837614331 22.925559086258605 48-49 23.178651286536653 26.676303645339665 27.256762048358112 22.888283019765566 50-51 22.796539789753968 26.757526800353503 27.459264135958826 22.986669273933703 52-53 23.17544400199359 26.827593222671016 27.074508609983027 22.922454165352367 54-55 22.868310310783205 26.509037349773873 27.30461858903716 23.31803375040576 56-57 23.001364128725875 26.667070506126432 27.188608683806727 23.142956681340966 58-59 23.26900688994549 26.506467900121685 27.19332662156852 23.031198588364305 60-61 23.143075821168605 26.51589840207321 27.537674274685948 22.803351502072243 62-63 22.949104455814854 26.64305044893544 27.443858559069273 22.963986536180432 64-65 22.718676887706984 26.73433721792003 27.336222974388306 23.210762919984678 66-67 22.80106708265499 26.729094193335317 27.09236665236414 23.377472071645556 68-69 22.701623919585572 26.545757273260477 27.3932660206752 23.359352786478745 70-71 22.885962684179976 26.447179353542836 27.264499609478793 23.402358352798394 72-73 22.917828007560622 26.40764763472921 27.328864220316714 23.34566013739346 74-75 22.670994588536082 26.849273074263134 27.18960502345007 23.290127313750716 76-77 22.85293971714093 26.549699830525764 27.097450392671174 23.49991005966213 78-79 22.87537828552899 26.595084164826183 27.141457167344697 23.388080382300135 80-81 22.97307145240311 26.626263601828605 27.099013117460096 23.30165182830819 82-83 22.88035923519971 26.582930590928555 27.293729102447656 23.242981071424076 84-85 22.93369406725299 26.600862875301658 27.09151544981169 23.37392760763366 86-87 22.835721349622297 26.59159820850846 27.17350657934504 23.399173862524204 88-89 22.742864018457727 26.550150469633003 26.986387251850257 23.720598260059013 90-91 22.944526819532967 26.364137322005394 27.09330325666594 23.598032601795698 92-93 23.08464570646515 26.36269587515753 27.12709994462071 23.42555847375661 94-95 22.93278405210559 26.257897198621823 27.231275651484577 23.578043097788015 96-97 22.828415441982184 26.115508753211735 27.41096330199345 23.645112502812626 98-99 22.830858855669117 26.18470296460098 27.04515132467093 23.939286855058974 100-101 23.098028217995832 25.55008757198013 27.42560333625386 23.926280873770178 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3038.0 1 1730.5 2 436.5 3 482.0 4 474.0 5 509.0 6 794.0 7 1457.0 8 2271.0 9 2814.0 10 2923.5 11 2905.0 12 3127.0 13 3430.0 14 3703.0 15 3996.5 16 4300.5 17 4453.5 18 4675.0 19 5029.5 20 5364.0 21 6008.5 22 6874.0 23 8296.0 24 10518.5 25 13242.5 26 16465.0 27 20308.0 28 24452.5 29 29047.5 30 34127.0 31 39840.0 32 46123.5 33 51175.5 34 56248.5 35 61776.0 36 68688.5 37 76171.5 38 81424.5 39 85170.5 40 89540.0 41 95730.5 42 101327.5 43 108070.5 44 115240.5 45 120838.5 46 125039.5 47 132357.5 48 146766.0 49 147881.5 50 136855.0 51 131022.5 52 127947.5 53 126409.0 54 123473.5 55 116038.0 56 107122.0 57 98525.0 58 90142.0 59 79952.5 60 66660.5 61 54174.0 62 44274.5 63 35166.0 64 26910.5 65 21073.0 66 16729.5 67 13868.5 68 11066.0 69 7931.5 70 5593.5 71 3981.0 72 3028.0 73 2468.5 74 1895.0 75 1467.5 76 1154.5 77 792.5 78 537.5 79 374.0 80 287.0 81 214.0 82 143.0 83 102.0 84 69.0 85 53.5 86 42.0 87 31.5 88 27.0 89 25.5 90 23.5 91 18.0 92 10.5 93 12.5 94 12.0 95 10.5 96 9.0 97 6.0 98 3.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3384910987327849 2 0.12056924764672948 3 0.11360716738880042 4 0.121210108590974 5 0.1385424841284961 6 0.08835142017698248 7 0.09691565279552282 8 0.1022464506499204 9 0.09039052318139686 10-11 0.08926901652896894 12-13 0.09330352747341739 14-15 0.09481828970526805 16-17 0.08417125901793304 18-19 0.09947909657250088 20-21 0.10910657575762873 22-23 0.11771450344054937 24-25 0.1313036684628251 26-27 0.13045889721813916 28-29 0.1252591845568825 30-31 0.11587931073657645 32-33 0.1090337506503282 34-35 0.3312377180456538 36-37 0.49036057749727485 38-39 0.4643037541051513 40-41 0.4422814416574761 42-43 0.4746594843632842 44-45 0.48210221032939665 46-47 0.48987993178909145 48-49 0.5016047740644741 50-51 0.4890642905873257 52-53 0.49330271183221563 54-55 0.479160075994456 56-57 0.4568318980961186 58-59 0.4370380339318392 60-61 0.39730465538867926 62-63 0.4666924176246081 64-65 0.4986335096866132 66-67 0.5069064418759515 68-69 0.5206412571128283 70-71 0.511945939301147 72-73 0.5036584420903486 74-75 0.5109409528204 76-77 0.48730192299065334 78-79 0.46565830110094086 80-81 0.4678867493843365 82-83 0.4810243987413491 84-85 0.4810389637628092 86-87 0.4842141384411116 88-89 0.49743917792688475 90-91 0.5129800558248142 92-93 0.50527515947242 94-95 0.5100524865113336 96-97 0.5091494551808072 98-99 0.5033380116182263 100-101 0.4904042725616552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3432882.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.149162246384876 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59335112817497 40.39167472741102 2 13.83382437471756 14.981800011091497 3 4.854931017786888 7.886713421314459 4 2.1693026733441343 4.698636896817119 5 1.2148039655761678 3.289030851476783 6 0.7577917163142738 2.4620271957400846 7 0.4946206689177175 1.874830639614863 8 0.35050645269462577 1.5183704620292895 9 0.27502315622696505 1.3403046157242107 >10 1.3359860944084374 12.899682177368218 >50 0.08311979680043315 3.076230907575599 >100 0.03398988714260763 3.3904169913928937 >500 0.0018327119337388316 0.6735464669392216 >1k 7.546460859494153E-4 0.8553596430887767 >5k 1.61709875560589E-4 0.6613749924159218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8488 0.24725580430670205 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7357 0.2143097257639499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6814 0.19849211245827847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4243 0.12359877211043083 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4073 0.11864666481399594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.913004292020524E-5 0.0 9 0.0 0.0 0.0 2.913004292020524E-5 0.0 10-11 0.0 0.0 0.0 5.826008584041048E-5 2.913004292020524E-5 12-13 0.0 0.0 0.0 5.826008584041048E-5 2.913004292020524E-5 14-15 0.0 0.0 0.0 5.826008584041048E-5 2.913004292020524E-5 16-17 0.0 0.0 0.0 5.826008584041048E-5 2.913004292020524E-5 18-19 0.0 0.0 0.0 5.826008584041048E-5 2.913004292020524E-5 20-21 0.0 0.0 0.0 8.739012876061571E-5 5.826008584041048E-5 22-23 0.0 0.0 0.0 1.6021523606112883E-4 5.826008584041048E-5 24-25 0.0 0.0 0.0 3.786905579626681E-4 5.826008584041048E-5 26-27 0.0 0.0 0.0 6.991210300849257E-4 5.826008584041048E-5 28-29 0.0 0.0 0.0 0.0020973630902547773 5.826008584041048E-5 30-31 0.0 0.0 0.0 0.005898833691341561 5.826008584041048E-5 32-33 0.0 0.0 0.0 0.014433936266961694 5.826008584041048E-5 34-35 0.0 0.0 0.0 0.028445486911580414 5.826008584041048E-5 36-37 0.0 0.0 0.0 0.051006705153279375 5.826008584041048E-5 38-39 0.0 0.0 0.0 0.08702600322411315 5.826008584041048E-5 40-41 0.0 0.0 0.0 0.12488049399891984 5.826008584041048E-5 42-43 0.0 0.0 0.0 0.1656479890657471 5.826008584041048E-5 44-45 0.0 0.0 0.0 0.21675664936924716 5.826008584041048E-5 46-47 0.0 0.0 0.0 0.27877451074636417 5.826008584041048E-5 48-49 0.0 0.0 0.0 0.3457299143984559 5.826008584041048E-5 50-51 0.0 0.0 0.0 0.42060868972484344 7.282510730051309E-5 52-53 0.0 0.0 0.0 0.4953272498151699 8.739012876061571E-5 54-55 0.0 0.0 0.0 0.5838680152711337 8.739012876061571E-5 56-57 0.0 0.0 0.0 0.6904839723590849 1.4565021460102618E-4 58-59 0.0 0.0 0.0 0.812553417216205 1.7478025752123142E-4 60-61 0.0 0.0 0.0 0.9446581618593357 1.7478025752123142E-4 62-63 0.0 0.0 0.0 1.0810158927688165 2.0391030044143667E-4 64-65 1.456502146010262E-5 0.0 0.0 1.2305112730353098 2.0391030044143667E-4 66-67 5.826008584041048E-5 0.0 0.0 1.4057430462218043 2.0391030044143667E-4 68-69 5.826008584041048E-5 0.0 0.0 1.6051818850749895 2.0391030044143667E-4 70-71 5.826008584041048E-5 0.0 0.0 1.8243708930280738 2.0391030044143667E-4 72-73 5.826008584041048E-5 0.0 0.0 2.0495461248012603 2.0391030044143667E-4 74-75 5.826008584041048E-5 0.0 0.0 2.2848294814677583 2.0391030044143667E-4 76-77 5.826008584041048E-5 0.0 0.0 2.5463881368482806 2.0391030044143667E-4 78-79 5.826008584041048E-5 0.0 0.0 2.8478112559651043 2.0391030044143667E-4 80-81 5.826008584041048E-5 0.0 0.0 3.181408507487295 2.0391030044143667E-4 82-83 5.826008584041048E-5 0.0 0.0 3.524036072314749 2.0391030044143667E-4 84-85 5.826008584041048E-5 0.0 0.0 3.872445950661864 2.0391030044143667E-4 86-87 5.826008584041048E-5 0.0 0.0 4.24245575583431 2.0391030044143667E-4 88-89 5.826008584041048E-5 0.0 0.0 4.653101970880444 2.0391030044143667E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11515 0.0 25.272058 1 TACATGG 11750 0.0 24.109488 2 ACATGGG 12045 0.0 22.771751 3 GTATCAA 18375 0.0 21.96017 1 GAGTACT 9130 0.0 21.732811 12-13 GTGTAGC 1910 0.0 20.629505 1 CATGGGG 7745 0.0 20.490288 4 GGTATCA 14320 0.0 20.421227 1 GTACTTT 10025 0.0 19.604912 14-15 AGAGTAC 15075 0.0 19.240305 10-11 ATCAACG 20740 0.0 19.163635 3 CAACGCA 20860 0.0 19.098812 5 TCAACGC 20860 0.0 19.098812 4 TATCAAC 21205 0.0 18.83276 2 AACGCAG 21200 0.0 18.83061 6 GTATAGC 910 0.0 18.258738 1 AGTACTT 9775 0.0 18.167166 12-13 GTATAGG 785 0.0 18.14244 1 TAGCCCT 2055 0.0 17.51969 4 ACGCAGA 23270 0.0 17.09447 7 >>END_MODULE