FastQCFastQC Report
Fri 27 May 2016
SRR939229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432882
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86890.2531109429336633No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79160.23059341975634468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.21390190516306704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45840.1335321167462208No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37500.10923766095076964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115850.023.2621351
TACATGG116750.022.433822
GAGTACT98550.021.6674912-13
ACATGGG119100.021.234233
GTATCAA198300.020.7693331
GTACTTT105400.020.14675714-15
GGTATCA157950.019.7447151
CATGGGG79200.019.5904144
TACCGTA2451.7788079E-719.3666737
AGAGTAC159850.019.3250710-11
AGTACTT104700.019.1257512-13
ATCAACG226650.018.0037843
TCAACGC227350.017.948354
TATCAAC228950.017.8850922
CAACGCA229350.017.7918385
AACGCAG232000.017.568166
GAGTACA95750.017.2054671
GTACCGT3903.0559022E-1017.032746
AGTACAT90700.016.2694992
ACTTTTT132150.016.06768216-17
Produced by FastQC (version 0.11.5)