##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939229_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3432882 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50495181599601 34.0 31.0 34.0 31.0 34.0 2 32.71477813685411 34.0 31.0 34.0 31.0 34.0 3 32.81150677477408 34.0 31.0 34.0 31.0 34.0 4 36.26309380864242 37.0 37.0 37.0 35.0 37.0 5 36.22051792051111 37.0 37.0 37.0 35.0 37.0 6 36.25674549838882 37.0 37.0 37.0 35.0 37.0 7 36.211248449553466 37.0 37.0 37.0 35.0 37.0 8 36.196381640848706 37.0 37.0 37.0 35.0 37.0 9 37.94740512490671 39.0 38.0 39.0 35.0 39.0 10-11 37.88497259736862 39.0 38.0 39.0 35.0 39.0 12-13 37.81654204834305 39.0 38.0 39.0 35.0 39.0 14-15 39.19205393602227 40.5 39.0 41.0 36.0 41.0 16-17 39.13514053206606 40.0 38.5 41.0 36.0 41.0 18-19 39.14876188578576 40.5 38.5 41.0 36.0 41.0 20-21 39.213536177474204 41.0 39.0 41.0 36.0 41.0 22-23 39.17451502848044 41.0 39.0 41.0 36.0 41.0 24-25 39.11771217303712 41.0 39.0 41.0 35.5 41.0 26-27 38.98133929450532 40.0 38.5 41.0 35.0 41.0 28-29 38.83157679174525 40.0 38.0 41.0 35.0 41.0 30-31 38.654651397863375 40.0 38.0 41.0 34.5 41.0 32-33 38.52800460371198 40.0 38.0 41.0 34.0 41.0 34-35 38.487076893409096 40.0 38.0 41.0 34.0 41.0 36-37 38.387122394536135 40.0 38.0 41.0 34.0 41.0 38-39 38.25398397031998 40.0 38.0 41.0 34.0 41.0 40-41 38.088181737676976 40.0 38.0 41.0 33.0 41.0 42-43 37.93193095480707 40.0 38.0 41.0 33.0 41.0 44-45 37.72782125922184 40.0 37.0 41.0 33.0 41.0 46-47 37.46480318869102 40.0 37.0 41.0 32.5 41.0 48-49 37.17222045499962 40.0 37.0 41.0 31.5 41.0 50-51 36.91915743098656 40.0 36.0 41.0 31.0 41.0 52-53 36.683487227350085 39.5 36.0 41.0 31.0 41.0 54-55 36.42947048573181 39.0 35.5 41.0 30.5 41.0 56-57 36.14818132403036 39.0 35.0 40.5 30.0 41.0 58-59 35.835201297335594 38.5 35.0 40.0 29.0 41.0 60-61 35.45976966292462 38.0 35.0 40.0 28.5 41.0 62-63 35.05806637105499 37.5 34.0 40.0 28.0 41.0 64-65 34.63890894006843 37.0 34.0 40.0 27.5 41.0 66-67 34.27139790997768 36.5 34.0 39.0 26.5 41.0 68-69 33.792699545163515 36.0 33.5 39.0 26.0 40.0 70-71 33.34181134102483 35.0 33.0 38.5 26.0 40.0 72-73 32.86782709105644 35.0 32.5 37.5 25.0 40.0 74-75 32.37223213614683 35.0 32.0 37.0 24.0 39.0 76-77 30.39845980724068 33.5 29.5 35.0 20.0 37.5 78-79 31.3841763276454 34.5 31.0 36.0 22.0 38.0 80-81 31.41150686216421 35.0 31.0 36.0 22.0 37.0 82-83 31.1267786075956 34.5 31.0 35.0 20.5 37.0 84-85 30.753060984910057 34.0 31.0 35.0 19.5 36.5 86-87 30.20520702430203 34.0 30.5 35.0 16.0 36.0 88-89 29.66590797469881 34.0 30.0 35.0 7.5 36.0 90-91 29.208671605956745 34.0 29.5 35.0 4.5 35.0 92-93 28.91642925681687 34.0 29.0 35.0 2.0 35.0 94-95 28.578451283790123 34.0 29.0 35.0 2.0 35.0 96-97 28.00831196644685 34.0 29.0 35.0 2.0 35.0 98-99 27.21784378257103 33.5 27.0 35.0 2.0 35.0 100-101 23.56771438692038 29.5 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1331051876424354 1101 2 0.15856504232902324 1101 3 0.16144782427523552 1101 4 0.06286443486598614 1101 5 0.12674492581322028 1101 6 0.11723247428981409 1101 7 0.08981081530173185 1101 8 0.10841181764865837 1101 9 0.20588456155788748 1101 10-11 0.15342032261177252 1101 12-13 0.1753343883741536 1101 14-15 0.20589550162577552 1101 16-17 0.2358331281292223 1101 18-19 0.2785813857811803 1101 20-21 0.2550963096663139 1101 22-23 0.23118823537391364 1101 24-25 0.15860645503908444 1101 26-27 0.1927932269510535 1101 28-29 0.13201720386050653 1101 30-31 0.15694703650546415 1101 32-33 0.09407682363019632 1101 34-35 0.1731384698407723 1101 36-37 0.1817683213645651 1101 38-39 0.30358941907272197 1101 40-41 0.2992303035270112 1101 42-43 0.27670210126945705 1101 44-45 0.2888787407686948 1101 46-47 0.16902296332682454 1101 48-49 0.2615546199841887 1101 50-51 0.36071308362274124 1101 52-53 0.3273078231959303 1101 54-55 0.35311955756677804 1101 56-57 0.29247210841568716 1101 58-59 0.3366761386757773 1101 60-61 0.31876312015894825 1101 62-63 0.1702315782518653 1101 64-65 0.15544381169537047 1101 66-67 0.19988840723429746 1101 68-69 0.17053487763993047 1101 70-71 0.1684416035674019 1101 72-73 0.11760426001745827 1101 74-75 0.1182252580797396 1101 76-77 0.07495949443895711 1101 78-79 0.18886396850527376 1101 80-81 0.10948609309035007 1101 82-83 0.09378451579885194 1101 84-85 0.24751879337227223 1101 86-87 0.2851335297369708 1101 88-89 0.47466371918402217 1101 90-91 0.45099207687810505 1101 92-93 0.3227875417179398 1101 94-95 0.12601898665730715 1101 96-97 -0.017250137561951817 1101 98-99 0.008261755752258182 1101 100-101 -0.4203586010911202 1102 1 0.02967489028792869 1102 2 0.01902831183204512 1102 3 0.06017006794765223 1102 4 0.03730503653034134 1102 5 0.051743241744510726 1102 6 0.05522763841121048 1102 7 0.04393755037170877 1102 8 0.05347941315451976 1102 9 0.1093011672968629 1102 10-11 0.11665559659468983 1102 12-13 0.09158891003018255 1102 14-15 0.10309551148372975 1102 16-17 0.13836388634350527 1102 18-19 0.06907436608989315 1102 20-21 0.08115884748940516 1102 22-23 0.13471809815654723 1102 24-25 0.09186771570827545 1102 26-27 0.08959574182699725 1102 28-29 -0.09052642447237247 1102 30-31 -0.01745670732988458 1102 32-33 -0.04441737249398159 1102 34-35 0.0024090079486427385 1102 36-37 0.07464402614047572 1102 38-39 0.12497260083987527 1102 40-41 0.05964170919041578 1102 42-43 0.00772702822469995 1102 44-45 0.06418706225877457 1102 46-47 0.04052309887381966 1102 48-49 0.08685617781620181 1102 50-51 0.09321385719901798 1102 52-53 0.024544143218271586 1102 54-55 0.011421687708313755 1102 56-57 -0.007104218233394022 1102 58-59 -0.060104250549464666 1102 60-61 -0.10143064358400977 1102 62-63 -0.06355160680127625 1102 64-65 -0.15069649450391864 1102 66-67 0.011752465922043598 1102 68-69 -0.14154419417806707 1102 70-71 -0.1223709938142079 1102 72-73 -0.15841777800689982 1102 74-75 -0.09744244093473498 1102 76-77 -0.1932984117844292 1102 78-79 -0.1290152002493663 1102 80-81 -0.25100116412307827 1102 82-83 -0.22980712355304078 1102 84-85 -0.1366271373426784 1102 86-87 -0.12172438141540098 1102 88-89 -0.1600554794731046 1102 90-91 0.06197001420884263 1102 92-93 0.037721151074240566 1102 94-95 -0.1683218305130758 1102 96-97 -0.2088930492756802 1102 98-99 -0.360538776577755 1102 100-101 -0.6525050904239826 1103 1 0.12410638431422427 1103 2 0.09883504639714147 1103 3 0.14900656413814772 1103 4 0.07728563697778412 1103 5 0.13191001531730961 1103 6 0.1001827639085846 1103 7 0.1647076917485535 1103 8 0.18356384292761874 1103 9 0.11085782494169649 1103 10-11 0.1989602842984013 1103 12-13 0.14787034956042788 1103 14-15 0.20919682721145705 1103 16-17 0.2233751245251696 1103 18-19 0.23259194302320196 1103 20-21 0.19826774051547602 1103 22-23 0.21202317698026008 1103 24-25 0.12785372925667815 1103 26-27 0.21034194665915606 1103 28-29 0.24409338080200627 1103 30-31 0.2997274371863554 1103 32-33 0.2939461813788924 1103 34-35 0.26781251342693224 1103 36-37 0.39789115662332364 1103 38-39 0.3299843605148851 1103 40-41 0.3043996059034413 1103 42-43 0.3244296779323932 1103 44-45 0.40436124103712245 1103 46-47 0.3832343427919085 1103 48-49 0.3258088627247062 1103 50-51 0.36851483708502286 1103 52-53 0.41918532971993727 1103 54-55 0.4564879434382263 1103 56-57 0.44039928880937396 1103 58-59 0.5029478935704361 1103 60-61 0.491879118249976 1103 62-63 0.35676228004509625 1103 64-65 0.314960873936343 1103 66-67 0.37666117851141223 1103 68-69 0.3312803702446132 1103 70-71 0.3447503107535468 1103 72-73 0.34904223649061805 1103 74-75 0.24950937829481035 1103 76-77 0.216188855955469 1103 78-79 0.37955288331473014 1103 80-81 0.4468735418850436 1103 82-83 0.5095260429024187 1103 84-85 0.4186960060106273 1103 86-87 0.567832818582513 1103 88-89 0.5981812472174468 1103 90-91 0.7523518516385046 1103 92-93 0.6612156780015503 1103 94-95 0.5297744058694285 1103 96-97 0.5660564962428687 1103 98-99 0.44849562633843476 1103 100-101 -0.19951286588980466 1104 1 0.02476429018097548 1104 2 0.03178533168060227 1104 3 0.05043350118256029 1104 4 0.022898439461506825 1104 5 0.06980488775658245 1104 6 0.06725271843322389 1104 7 0.03562704171848452 1104 8 0.03184518155958216 1104 9 0.07140212291080417 1104 10-11 0.06248863929118187 1104 12-13 0.09079419264545407 1104 14-15 0.0592761558449908 1104 16-17 0.06046158682480751 1104 18-19 0.1315150955496165 1104 20-21 0.1074184675689196 1104 22-23 0.1350741476655628 1104 24-25 0.0911044646057988 1104 26-27 0.09810543359693469 1104 28-29 0.03562035100872407 1104 30-31 0.12688579462706429 1104 32-33 0.04843048105605163 1104 34-35 0.13884773782688598 1104 36-37 0.1727196227210399 1104 38-39 0.19861098660409482 1104 40-41 0.2777428818131966 1104 42-43 0.218130277157087 1104 44-45 0.29467044945680243 1104 46-47 0.25488970431299407 1104 48-49 0.1340951278034126 1104 50-51 0.1221189700823686 1104 52-53 0.06947706423480327 1104 54-55 0.10612682694273445 1104 56-57 0.19773647402481487 1104 58-59 0.2371811238295365 1104 60-61 0.2681067400643755 1104 62-63 0.3220706273047682 1104 64-65 0.16215011899558363 1104 66-67 0.13516306990415217 1104 68-69 0.0036899041114324405 1104 70-71 0.08620612570340569 1104 72-73 -0.019176688932518005 1104 74-75 -0.047163507068184174 1104 76-77 -0.01972776378052643 1104 78-79 0.09307893508665188 1104 80-81 0.053275925168311034 1104 82-83 0.0994236576012888 1104 84-85 -0.008374136032870183 1104 86-87 0.07857958597127279 1104 88-89 0.23919971517607763 1104 90-91 0.2952335946997451 1104 92-93 0.2701945093635878 1104 94-95 0.12458771555092696 1104 96-97 0.05247860161909301 1104 98-99 0.23044109585204353 1104 100-101 -0.21741414046777052 1105 1 0.1133688526194021 1105 2 0.14754986037041107 1105 3 0.13927703331197705 1105 4 0.14430251340166222 1105 5 0.11437647058463796 1105 6 0.16056086979995143 1105 7 0.18262348456647715 1105 8 0.19031964049096217 1105 9 0.18033106254879527 1105 10-11 0.1996219293928192 1105 12-13 0.19221102690965353 1105 14-15 0.18457651156019494 1105 16-17 0.2248549534485349 1105 18-19 0.22811787094500602 1105 20-21 0.23391262581088768 1105 22-23 0.23852929677009627 1105 24-25 0.23036377809837205 1105 26-27 0.26068247118958965 1105 28-29 0.28355773078924074 1105 30-31 0.28794329776734884 1105 32-33 0.25314054477798464 1105 34-35 0.3329421781706259 1105 36-37 0.2111733224679213 1105 38-39 0.3063101139015245 1105 40-41 0.3616854434208534 1105 42-43 0.3013376508512948 1105 44-45 0.27453010581460546 1105 46-47 0.27537744553640664 1105 48-49 0.24570114557622702 1105 50-51 0.2486536206875627 1105 52-53 0.15648618771709266 1105 54-55 0.19911875934987933 1105 56-57 0.24899283301925124 1105 58-59 0.37003796215793017 1105 60-61 0.44320069928114236 1105 62-63 0.33820133047731105 1105 64-65 0.20540004702576198 1105 66-67 0.1735576498046001 1105 68-69 0.12564035102161597 1105 70-71 0.09890688166046857 1105 72-73 0.08137909739131999 1105 74-75 0.07482200333979705 1105 76-77 0.09541887037271835 1105 78-79 0.19548183061435864 1105 80-81 0.16060803127922085 1105 82-83 0.13800794895504964 1105 84-85 0.12375209654370778 1105 86-87 0.1746379806408207 1105 88-89 0.25001919494135194 1105 90-91 0.2795847655914656 1105 92-93 0.2280501772485124 1105 94-95 0.09796356337534817 1105 96-97 0.14936063984428571 1105 98-99 0.053060887005088375 1105 100-101 -0.13395933004474614 1106 1 0.026666538678526308 1106 2 0.042783508512073354 1106 3 0.09664304521148637 1106 4 0.008017840116139041 1106 5 0.03423188842933911 1106 6 0.03968699908111262 1106 7 0.04646503831688875 1106 8 -0.009320849725490632 1106 9 0.06004808621019464 1106 10-11 0.08755049627938405 1106 12-13 0.10516149371242278 1106 14-15 0.04556746971830705 1106 16-17 0.06068136564909565 1106 18-19 0.060799996152738345 1106 20-21 0.1379830206357724 1106 22-23 0.11563389532540924 1106 24-25 0.17468954065677167 1106 26-27 0.19456698009523166 1106 28-29 0.23333037976324533 1106 30-31 0.12825281025575208 1106 32-33 0.18338824398180265 1106 34-35 0.015532480327777876 1106 36-37 0.12445905017627723 1106 38-39 0.11324386938362352 1106 40-41 0.022525118950937895 1106 42-43 0.12540497067169554 1106 44-45 0.08890162330084195 1106 46-47 0.12209653913138396 1106 48-49 0.11289606764289317 1106 50-51 0.07922775439521246 1106 52-53 0.12756534345752613 1106 54-55 0.02336559679318384 1106 56-57 0.10302493264939727 1106 58-59 0.054618950088531903 1106 60-61 -0.009986058838435952 1106 62-63 0.1440632001369906 1106 64-65 0.22893503042168106 1106 66-67 0.10089315841888435 1106 68-69 0.14909978801107826 1106 70-71 0.17924630900731842 1106 72-73 0.1739640182906257 1106 74-75 0.16614764346747535 1106 76-77 0.1639429726185142 1106 78-79 0.2552907152893553 1106 80-81 0.11622257187449847 1106 82-83 0.1335088567457241 1106 84-85 0.11769376826419986 1106 86-87 0.07250687915333742 1106 88-89 0.34081069540117426 1106 90-91 0.4373877792856753 1106 92-93 0.25276704431246344 1106 94-95 0.05430106220086728 1106 96-97 0.19857466300362603 1106 98-99 0.33339903310006846 1106 100-101 0.3540744418563584 1107 1 0.05935835684323365 1107 2 0.06889802050209681 1107 3 -0.013579171917832866 1107 4 0.012732506875934746 1107 5 -0.0026634870028630075 1107 6 0.05682180839316686 1107 7 0.051577042687327435 1107 8 0.024245311261083202 1107 9 0.06106284772532433 1107 10-11 0.05577767480856721 1107 12-13 0.03605735315257874 1107 14-15 0.161436037093857 1107 16-17 0.061942587435417806 1107 18-19 0.0985220138856846 1107 20-21 0.11273453002304024 1107 22-23 0.11940487389183119 1107 24-25 0.07013072591422542 1107 26-27 0.12497974102595322 1107 28-29 0.0322286205007245 1107 30-31 0.10270970412695846 1107 32-33 0.13210718259836796 1107 34-35 0.11798701545595947 1107 36-37 0.15943571875551044 1107 38-39 0.23882460844976805 1107 40-41 0.2255054784318915 1107 42-43 0.21808512790061485 1107 44-45 0.133957471107351 1107 46-47 0.2524710214170298 1107 48-49 0.09466169083678722 1107 50-51 0.029689421774435232 1107 52-53 0.06026804770874605 1107 54-55 0.08357138475350467 1107 56-57 0.09881660078254839 1107 58-59 0.04558104277086983 1107 60-61 0.1311395024839257 1107 62-63 -0.005766046548551174 1107 64-65 -0.09330026965552207 1107 66-67 0.017742692240645397 1107 68-69 -0.0351094387521087 1107 70-71 -0.046707558177153885 1107 72-73 -0.18786567002261734 1107 74-75 -0.14476703607915908 1107 76-77 -0.005526747645436814 1107 78-79 0.23488821565472406 1107 80-81 0.12871847140310777 1107 82-83 0.22263803075731659 1107 84-85 0.31248378002062793 1107 86-87 0.3242786420272701 1107 88-89 0.2458507351913255 1107 90-91 0.18269964565655172 1107 92-93 0.21925626867834325 1107 94-95 0.23520162803181321 1107 96-97 0.23153072113805706 1107 98-99 0.32526388883850643 1107 100-101 -0.11919207618840133 1108 1 -0.07019057277950935 1108 2 0.008605316143011521 1108 3 -0.026282786260601654 1108 4 0.0049377651707942505 1108 5 -0.058624492537816764 1108 6 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-0.5700119420950607 2316 94-95 -0.35837322410643324 2316 96-97 -0.4600202342586286 2316 98-99 -0.3978135944722432 2316 100-101 -0.12794752469040205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 26.0 5 50.0 6 120.0 7 302.0 8 651.0 9 1400.0 10 2463.0 11 3773.0 12 5448.0 13 8113.0 14 10066.0 15 13243.0 16 16969.0 17 23465.0 18 23183.0 19 17040.0 20 16383.0 21 17056.0 22 17912.0 23 20022.0 24 23259.0 25 27054.0 26 31877.0 27 38969.0 28 47101.0 29 57583.0 30 70528.0 31 87091.0 32 109024.0 33 142322.0 34 198600.0 35 292612.0 36 481078.0 37 827941.0 38 729211.0 39 70929.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.899393409348136 18.915064447333712 14.513862256685591 23.671679886632557 2 15.75300287047443 20.033254856997708 40.04195308781368 24.171789184714186 3 20.085455893910716 23.506196834030415 29.237445388452038 27.170901883606835 4 13.626567997385287 16.190536115135913 36.003130897013065 34.17976499046574 5 14.373957508588994 36.79217636959266 33.70415877970754 15.129707342110798 6 33.22255178010779 35.09293357592833 16.839611731483924 14.844902912479952 7 29.415255170436964 31.94438375685503 20.1175863312517 18.522774741456306 8 26.146922614875777 35.66353285664931 19.092383600717998 19.097160927756914 9 26.084875623455744 17.428912499759676 20.204656029540192 36.281555847244384 10-11 25.624664640380878 26.140062489768074 26.643968537223245 21.591304332627804 12-13 26.357212394716743 23.3166622097701 27.37995363662369 22.94617175888947 14-15 22.869473722846536 24.919578460922693 25.715011059327107 26.49593675690367 16-17 22.666030456243565 28.118301347300832 26.34525805484198 22.870410141613622 18-19 22.60657561676721 26.948892322028783 28.272999387249104 22.17153267395491 20-21 23.222149785515494 26.006661458214992 28.340298326595555 22.43089042967396 22-23 22.902913645152964 25.894685573229726 28.36542298861423 22.83697779300308 24-25 22.94869651189414 26.06284369969582 28.344843637939842 22.643616150470187 26-27 22.745068737486683 26.177369602979223 28.459049831291438 22.618511828242653 28-29 22.517319267018205 26.67956544967173 28.270939694402546 22.532175588907513 30-31 22.760470103770302 26.132480611889974 28.203167467476742 22.90388181686298 32-33 22.56191736272904 26.28748963698723 28.29268527144248 22.857907728841248 34-35 23.14387555480457 26.24693555599903 28.076832876185538 22.53235601301087 36-37 23.026267097681806 26.115694580666442 28.201755996244643 22.656282325407105 38-39 22.784244181074705 26.45836426033134 28.191189087046673 22.566202471547285 40-41 22.990109803987835 26.366038348217526 28.010476085315506 22.633375762479137 42-43 22.724368017780556 26.44540218122959 27.7412892913604 23.08894050962946 44-45 22.902280505580116 26.361309723825034 27.76618121315748 22.97022855743737 46-47 23.11288793703635 26.64307200048473 27.434224976535447 22.809815085943466 48-49 23.124342657492384 26.69390637214227 27.33341751978272 22.84833345058263 50-51 22.74309697487153 26.83629396783283 27.487176215648727 22.93343284164691 52-53 23.155644823005755 26.833067278786327 27.11080783482091 22.90048006338701 54-55 22.861898544721313 26.54017819429855 27.28571794777682 23.312205313203307 56-57 22.95871224236662 26.742500907400835 27.199638670947618 23.099148179284928 58-59 23.256561545220013 26.576122547431662 27.195090901463793 22.972225005884532 60-61 23.053097815644964 26.617283173735007 27.540102784438332 22.7895162261817 62-63 22.917838599663998 26.66456240665199 27.436108227909127 22.981490765774886 64-65 22.705704741242762 26.79510400030296 27.2772610716754 23.221930186778877 66-67 22.790217141312343 26.766599125445328 27.071896515837228 23.371287217405097 68-69 22.658978767485234 26.619210115260227 27.385970564741896 23.335840552512646 70-71 22.905512891358597 26.535321206881335 27.230757489117742 23.328408412642325 72-73 22.83157417004138 26.503066519618212 27.304725300782255 23.36063400955815 74-75 22.63478616509394 26.925175406553443 27.077103145403775 23.362935282948847 76-77 22.777387320124916 26.587830712158265 27.103789482749434 23.53099248496738 78-79 22.77281901941553 26.623983249006443 27.191103306823937 23.41209442475409 80-81 22.891204583367987 26.70771139079216 27.118022686995698 23.283061338844156 82-83 22.773412295560703 26.644013091106117 27.297359494183432 23.28521511914975 84-85 22.78061447795387 26.68213277970708 27.06670766235304 23.47054507998601 86-87 22.703478797063827 26.686058201725526 27.15169708495624 23.458765916254407 88-89 22.608127227858514 26.669021113243762 26.93966547847546 23.783186180422263 90-91 22.74763027544735 26.523192776589717 27.044813657217016 23.684363290745917 92-93 22.864185253090554 26.48586522927383 27.086570409352838 23.563379108282778 94-95 22.61675466852633 26.421181968969513 27.17576660077451 23.78629676172965 96-97 22.477178067874164 26.31676533012204 27.341618500140697 23.8644381018631 98-99 22.440282047625796 26.39051311827913 26.98572268987244 24.183482144222637 100-101 22.60537112773058 25.702924490391304 27.50721010750674 24.18449427437137 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 369.0 1 278.0 2 262.5 3 349.5 4 326.0 5 352.0 6 585.5 7 1191.5 8 1981.0 9 2463.5 10 2521.5 11 2513.0 12 2636.5 13 2792.5 14 2942.5 15 3127.5 16 3350.0 17 3562.0 18 3858.0 19 4246.5 20 4793.0 21 5738.0 22 6813.0 23 8336.5 24 10682.5 25 13529.5 26 16690.0 27 20548.0 28 24987.0 29 29595.0 30 34734.0 31 40519.0 32 46739.5 33 51819.0 34 57015.0 35 63003.5 36 69171.5 37 76120.5 38 81876.5 39 85643.5 40 89943.0 41 96213.0 42 102117.0 43 108916.0 44 116071.0 45 121729.0 46 125786.0 47 133646.5 48 148440.0 49 149199.0 50 138021.5 51 132178.0 52 128434.0 53 126808.0 54 124165.5 55 116321.5 56 107411.5 57 98977.5 58 90039.0 59 79432.5 60 66190.0 61 53442.0 62 43485.0 63 34412.5 64 26256.5 65 20473.0 66 16138.5 67 13240.0 68 10447.0 69 7336.0 70 5129.0 71 3773.0 72 2850.5 73 2256.0 74 1718.5 75 1291.0 76 952.5 77 601.5 78 414.5 79 276.5 80 166.0 81 124.0 82 76.0 83 43.0 84 22.5 85 13.5 86 10.0 87 5.0 88 3.0 89 2.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3435597262009006 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.612914163667728E-4 16-17 0.0016167173820713907 18-19 7.28251073005131E-5 20-21 0.0 22-23 0.0 24-25 4.660806867232838E-4 26-27 0.0011360716738880044 28-29 0.0 30-31 0.0031460446353821655 32-33 0.0 34-35 0.010690725751715324 36-37 0.028663962233481957 38-39 0.02157079678241198 40-41 0.014361111159661185 42-43 0.01073442081609563 44-45 0.0032771298285230897 46-47 0.0012817218884890304 48-49 7.428160944652336E-4 50-51 8.739012876061571E-5 52-53 5.826008584041048E-5 54-55 0.0 56-57 0.0 58-59 0.004486026609711607 60-61 0.010443120386893578 62-63 0.004748196995993454 64-65 0.0023158384121563166 66-67 0.004762762017453556 68-69 0.0037286454937862706 70-71 2.913004292020524E-5 72-73 0.0 74-75 0.0 76-77 0.21598470323186172 78-79 3.204304721222576E-4 80-81 0.0035684302577251416 82-83 4.95210729643489E-4 84-85 1.456502146010262E-5 86-87 0.10843658477046401 88-89 0.40256262813577626 90-91 0.3916388620406993 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0016749774679118011 100-101 8.010761803056441E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3432882.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.2470921743086 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81905904629076 38.04584090037974 2 14.653171800389034 15.311712354018105 3 5.2973641598948165 8.303156206287097 4 2.3397987658084385 4.889907271461079 5 1.306488230206254 3.4130105494117733 6 0.8251812734583605 2.5867993232935387 7 0.5348033699328095 1.9559344674807255 8 0.3730853970316772 1.5594101702082046 9 0.2692051817562608 1.265866915051877 >10 1.4532973745859772 13.609652042068824 >50 0.08773508243520807 3.1641225813347966 >100 0.037962959658671365 3.633690950477214 >500 0.0017307473586622676 0.5990027483692033 >1k 9.491195141208355E-4 0.9633340969092794 >5k 1.674916789625004E-4 0.6985594232485518 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8689 0.2531109429336633 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7916 0.23059341975634468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7343 0.21390190516306704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4584 0.1335321167462208 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3750 0.10923766095076964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.913004292020524E-5 0.0 9 0.0 0.0 0.0 8.739012876061571E-5 2.913004292020524E-5 10-11 0.0 0.0 0.0 8.739012876061571E-5 2.913004292020524E-5 12-13 0.0 0.0 0.0 8.739012876061571E-5 2.913004292020524E-5 14-15 0.0 0.0 0.0 8.739012876061571E-5 5.826008584041048E-5 16-17 0.0 0.0 0.0 8.739012876061571E-5 5.826008584041048E-5 18-19 0.0 0.0 0.0 8.739012876061571E-5 5.826008584041048E-5 20-21 0.0 0.0 0.0 1.1652017168082096E-4 5.826008584041048E-5 22-23 0.0 0.0 0.0 1.8934527898133407E-4 5.826008584041048E-5 24-25 0.0 0.0 0.0 4.0782060088287333E-4 5.826008584041048E-5 26-27 0.0 0.0 0.0 7.28251073005131E-4 5.826008584041048E-5 28-29 0.0 0.0 0.0 0.0021701881975552902 5.826008584041048E-5 30-31 0.0 0.0 0.0 0.005898833691341561 5.826008584041048E-5 32-33 0.0 0.0 0.0 0.01456502146010262 5.826008584041048E-5 34-35 0.0 0.0 0.0 0.028838742491003187 5.826008584041048E-5 36-37 0.0 0.0 0.0 0.05151648090438296 5.826008584041048E-5 38-39 0.0 0.0 0.0 0.08766686416835767 1.1652017168082096E-4 40-41 0.0 0.0 0.0 0.12585635043674673 1.1652017168082096E-4 42-43 0.0 0.0 0.0 0.16701710108299672 1.3108519314092358E-4 44-45 0.0 0.0 0.0 0.21878118735220142 1.456502146010262E-4 46-47 0.0 0.0 0.0 0.2816583849954645 1.456502146010262E-4 48-49 0.0 0.0 0.0 0.3492837796347209 1.456502146010262E-4 50-51 0.0 0.0 0.0 0.42515297642039546 1.456502146010262E-4 52-53 0.0 0.0 0.0 0.5002356620472245 1.6021523606112883E-4 54-55 0.0 0.0 0.0 0.5888055575461085 2.0391030044143667E-4 56-57 0.0 0.0 0.0 0.6959458554066233 2.330403433616419E-4 58-59 0.0 0.0 0.0 0.818816376444049 2.330403433616419E-4 60-61 0.0 0.0 0.0 0.952086322803988 2.330403433616419E-4 62-63 0.0 0.0 0.0 1.0898277307521784 2.330403433616419E-4 64-65 0.0 0.0 0.0 1.2408233082290623 2.330403433616419E-4 66-67 0.0 0.0 0.0 1.4168415925744027 2.330403433616419E-4 68-69 0.0 0.0 0.0 1.617460198165856 2.6217038628184716E-4 70-71 0.0 0.0 0.0 1.8381785333722511 2.6217038628184716E-4 72-73 0.0 0.0 0.0 2.0657278636434344 2.6217038628184716E-4 74-75 0.0 0.0 0.0 2.3040698748165536 2.6217038628184716E-4 76-77 0.0 0.0 0.0 2.567711328265871 2.6217038628184716E-4 78-79 0.0 0.0 0.0 2.871683326138213 2.6217038628184716E-4 80-81 0.0 2.913004292020524E-5 0.0 3.2067807748707935 2.6217038628184716E-4 82-83 0.0 2.913004292020524E-5 0.0 3.5499181154493513 2.6217038628184716E-4 84-85 0.0 2.913004292020524E-5 0.0 3.9005855721227816 2.6217038628184716E-4 86-87 0.0 2.913004292020524E-5 0.0 4.27219752965584 2.6217038628184716E-4 88-89 0.0 2.913004292020524E-5 0.0 4.6846206773201065 2.6217038628184716E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11585 0.0 23.262135 1 TACATGG 11675 0.0 22.43382 2 GAGTACT 9855 0.0 21.66749 12-13 ACATGGG 11910 0.0 21.23423 3 GTATCAA 19830 0.0 20.769333 1 GTACTTT 10540 0.0 20.146757 14-15 GGTATCA 15795 0.0 19.744715 1 CATGGGG 7920 0.0 19.590414 4 TACCGTA 245 1.7788079E-7 19.366673 7 AGAGTAC 15985 0.0 19.32507 10-11 AGTACTT 10470 0.0 19.12575 12-13 ATCAACG 22665 0.0 18.003784 3 TCAACGC 22735 0.0 17.94835 4 TATCAAC 22895 0.0 17.885092 2 CAACGCA 22935 0.0 17.791838 5 AACGCAG 23200 0.0 17.56816 6 GAGTACA 9575 0.0 17.205467 1 GTACCGT 390 3.0559022E-10 17.03274 6 AGTACAT 9070 0.0 16.269499 2 ACTTTTT 13215 0.0 16.067682 16-17 >>END_MODULE