FastQCFastQC Report
Fri 27 May 2016
SRR939228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375335
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81220.24062796729806077No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72750.21553416179431079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64400.19079587655743802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40690.12055099716028186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40270.11930667622621163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118150.024.5586531
TACATGG117200.024.0074652
ACATGGG117650.022.7883013
GTATCAA179050.022.7302131
GAGTACT90600.021.293112-13
CATGGGG72800.020.2356174
GGTATCA138700.019.8243581
ATCAACG203750.019.6680013
CAACGCA204950.019.5756655
AGAGTAC149600.019.57154310-11
GTACTTT98350.019.5677914-15
TCAACGC205900.019.5086384
TATCAAC206300.019.4478532
AACGCAG209600.019.2200816
GTGTAGC19100.019.1449571
AGTACTT97600.018.8443312-13
ACGCAGA228300.017.5420957
GAGTACA92300.017.2875981
CGCAGAG231200.017.2399028
GTGTAGG16100.017.1079961
Produced by FastQC (version 0.11.5)