FastQCFastQC Report
Fri 27 May 2016
SRR939228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375335
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84470.25025664119265195No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78760.2333398018270779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73610.21808205704026412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.12801692276470336No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38060.1127591779778896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112250.023.2664641
GTATCAA185200.022.6195561
TACATGG112900.022.2338812
GGTATCA142650.021.214761
ACATGGG115150.020.9752623
GAGTACT103750.020.65198512-13
ATTCGCG4350.019.65477480-81
AGTACTT106950.019.54597312-13
GTAAGCG3650.019.5007894-95
GTACTTT108100.019.46973614-15
TATCAAC214250.019.379422
ATCAACG212050.019.3343263
AGAGTAC161200.019.26786210-11
TCAACGC214150.019.1447284
CAACGCA214950.019.0293245
AACGCAG218550.018.715876
GTGTAGC20600.018.5079781
ATACCGT3851.2732926E-1118.4877516
CATGGGG71800.018.3727384
CGCGGAA4200.018.1738382-83
Produced by FastQC (version 0.11.5)