##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939227_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73778 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08223318604462 33.0 31.0 34.0 30.0 34.0 2 32.21819512591829 33.0 31.0 34.0 30.0 34.0 3 32.24275529290574 34.0 31.0 34.0 30.0 34.0 4 35.8141586922931 37.0 35.0 37.0 35.0 37.0 5 35.820081867223294 37.0 35.0 37.0 35.0 37.0 6 35.95886307571363 37.0 35.0 37.0 35.0 37.0 7 35.900796985551246 37.0 35.0 37.0 35.0 37.0 8 35.959513676163624 37.0 35.0 37.0 35.0 37.0 9 37.50223643904687 39.0 37.0 39.0 35.0 39.0 10-11 37.47813711404484 39.0 37.0 39.0 34.5 39.0 12-13 37.43028409552983 39.0 37.0 39.0 34.0 39.0 14-15 38.35348613407791 40.0 38.0 41.0 33.5 41.0 16-17 38.330186505462336 40.0 37.5 41.0 33.5 41.0 18-19 38.51801349995934 40.0 38.0 41.0 34.0 41.0 20-21 38.62895443086015 40.0 38.0 41.0 34.0 41.0 22-23 38.51833202309632 40.0 38.0 41.0 34.0 41.0 24-25 38.31559543495351 40.0 37.0 41.0 34.0 41.0 26-27 37.94967334435739 40.0 36.5 41.0 33.5 41.0 28-29 37.646107240640845 40.0 35.5 41.0 33.0 41.0 30-31 37.1488587383773 39.0 35.0 41.0 33.0 41.0 32-33 36.39730678522052 38.0 35.0 41.0 32.0 41.0 34-35 35.85275420857166 38.0 35.0 40.0 31.0 41.0 36-37 35.40833988451842 37.5 35.0 40.5 30.5 41.0 38-39 34.95575239231207 37.0 35.0 40.0 28.5 41.0 40-41 34.60834530618884 37.0 35.0 40.0 26.0 41.0 42-43 34.03718588197023 36.5 35.0 40.0 22.5 41.0 44-45 33.312498305728 36.0 33.5 40.0 12.5 41.0 46-47 32.37208246360704 36.0 33.0 40.0 2.0 41.0 48-49 31.38887608772263 35.0 33.0 40.0 2.0 41.0 50-51 30.650939304399685 35.0 31.0 40.0 2.0 41.0 52-53 30.18630892678034 35.0 30.5 40.0 2.0 41.0 54-55 29.69242863726314 35.0 28.5 40.0 2.0 41.0 56-57 28.89088210577679 35.0 25.0 39.5 2.0 41.0 58-59 28.193119900241264 35.0 21.0 39.0 2.0 41.0 60-61 27.259975873566646 35.0 7.0 39.0 2.0 40.5 62-63 26.611151020629457 35.0 2.0 38.0 2.0 40.0 64-65 25.874867846783594 35.0 2.0 37.5 2.0 39.5 66-67 25.07676407601182 34.0 2.0 37.0 2.0 39.0 68-69 24.34513676163626 33.5 2.0 36.0 2.0 39.0 70-71 23.696698202716256 33.0 2.0 35.5 2.0 38.0 72-73 23.13247173954295 33.0 2.0 35.0 2.0 37.0 74-75 22.70804982515113 32.5 2.0 35.0 2.0 37.0 76-77 21.19298435848085 29.5 2.0 33.5 2.0 35.0 78-79 21.821796470492558 31.0 2.0 35.0 2.0 36.0 80-81 21.716385643416736 31.0 2.0 35.0 2.0 35.0 82-83 21.444753178454285 31.0 2.0 35.0 2.0 35.0 84-85 21.1054040499878 31.0 2.0 35.0 2.0 35.0 86-87 20.77430941473068 30.5 2.0 35.0 2.0 35.0 88-89 20.27408577082599 29.5 2.0 34.0 2.0 35.0 90-91 19.843869446176367 29.0 2.0 34.0 2.0 35.0 92-93 19.625789530754425 29.0 2.0 34.0 2.0 35.0 94-95 19.288920816503566 28.0 2.0 34.0 2.0 35.0 96-97 18.788832714359295 27.0 2.0 34.0 2.0 35.0 98-99 18.23725907452086 25.0 2.0 34.0 2.0 35.0 100-101 15.837471875084713 10.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5099788301760952 1101 2 0.31253068528742034 1101 3 0.5486359635917353 1101 4 0.5429571364506032 1101 5 0.720480581099828 1101 6 0.5748608866194616 1101 7 0.3994376546162428 1101 8 0.5933358187837996 1101 9 0.6198728074904807 1101 10-11 0.3443762235148853 1101 12-13 0.6589836978120474 1101 14-15 0.007300771346393731 1101 16-17 0.3640900605722024 1101 18-19 0.3722352958763153 1101 20-21 0.4717641904323031 1101 22-23 0.6741086408487504 1101 24-25 0.6701841017052246 1101 26-27 0.6179809210240919 1101 28-29 0.5773118379903366 1101 30-31 0.9655330026219175 1101 32-33 1.297350291050769 1101 34-35 2.0006216253160503 1101 36-37 2.480728489821601 1101 38-39 2.3154705975999974 1101 40-41 2.77330988289453 1101 42-43 3.422469256085222 1101 44-45 3.8072408019198463 1101 46-47 3.4344136565543266 1101 48-49 3.293110497379626 1101 50-51 2.9538733131044133 1101 52-53 3.3836429734406757 1101 54-55 2.7260513288430452 1101 56-57 2.286513307502169 1101 58-59 1.706552594566059 1101 60-61 2.4440230753011427 1101 62-63 2.9022173162185894 1101 64-65 3.1798315050475523 1101 66-67 2.2489873509848515 1101 68-69 1.877665899947683 1101 70-71 2.0542908823972112 1101 72-73 2.4056809085048236 1101 74-75 3.059596556673789 1101 76-77 2.047374868349692 1101 78-79 2.151485452063323 1101 80-81 2.937438294698506 1101 82-83 2.357354631448981 1101 84-85 1.593417623222539 1101 86-87 1.7648374879193653 1101 88-89 1.5032202636159049 1101 90-91 1.7446494535330181 1101 92-93 1.5143775162971558 1101 94-95 1.5368252509245437 1101 96-97 1.4236123642460647 1101 98-99 0.7950190838616322 1101 100-101 0.3705914774123542 1102 1 0.13921380285368912 1102 2 -0.6800922655322488 1102 3 0.010657821515231092 1102 4 -0.10239805480622977 1102 5 -0.07569428228815411 1102 6 0.09070788115498374 1102 7 0.2797655234687042 1102 8 0.0919696985652152 1102 9 -0.15280478813793508 1102 10-11 0.2976549120394836 1102 12-13 -0.15467750437374406 1102 14-15 -0.08122381881754137 1102 16-17 0.06490973270334877 1102 18-19 0.07797300079434422 1102 20-21 0.16548003742683903 1102 22-23 0.06386273920941221 1102 24-25 0.1868507683718903 1102 26-27 -0.8671283685933986 1102 28-29 -0.37678652266539814 1102 30-31 -0.3565981449190687 1102 32-33 0.5387437336737193 1102 34-35 0.9860041389772576 1102 36-37 1.3927503477450998 1102 38-39 0.46697332984043527 1102 40-41 1.3812333801622856 1102 42-43 1.750611332587951 1102 44-45 1.4443992718652012 1102 46-47 1.8975284106526829 1102 48-49 2.6444766175982117 1102 50-51 2.4454033677492255 1102 52-53 2.3519489843696313 1102 54-55 1.890942039225564 1102 56-57 1.4306389905622794 1102 58-59 0.5860607912873697 1102 60-61 0.44074438677654726 1102 62-63 1.1395123981858006 1102 64-65 1.0889845105120344 1102 66-67 0.2116922690176395 1102 68-69 1.2196057906580684 1102 70-71 0.829973942506502 1102 72-73 1.2507628757179354 1102 74-75 1.155224971974338 1102 76-77 0.5771562891147219 1102 78-79 1.1768952881288932 1102 80-81 1.2148973110919492 1102 82-83 1.427846434727666 1102 84-85 1.4113137980859243 1102 86-87 0.33901781578821755 1102 88-89 -0.31973055605622847 1102 90-91 -0.17433961750523252 1102 92-93 -0.020458549276614946 1102 94-95 0.0972077645857432 1102 96-97 -0.8496116794697812 1102 98-99 -0.11358747351541609 1102 100-101 -0.04143038051114267 1103 1 0.1433121635094281 1103 2 -0.04996931471257682 1103 3 0.2007192969250653 1103 4 0.020040469783936032 1103 5 -0.01806108556684194 1103 6 0.2915275532861301 1103 7 0.09943765461624565 1103 8 0.4631274854504639 1103 9 -0.3415855258428522 1103 10-11 0.40062622351489097 1103 12-13 0.5022128644787145 1103 14-15 0.29480077134639515 1103 16-17 0.4812775605722024 1103 18-19 0.5550477958763125 1103 20-21 0.694680857098966 1103 22-23 0.4803586408487561 1103 24-25 0.0852882683718903 1103 26-27 0.2997517543574233 1103 28-29 0.7679368379903337 1103 30-31 0.5030330026219119 1103 32-33 0.6786002910507634 1103 34-35 0.6011424586493845 1103 36-37 1.106249323154934 1103 38-39 0.6899497642666645 1103 40-41 1.1613307162278588 1103 42-43 0.02507342275188762 1103 44-45 -0.1464050314134866 1103 46-47 0.09847615655432662 1103 48-49 -0.40636866928703697 1103 50-51 0.47626914643774754 1103 52-53 0.23155964010733854 1103 54-55 0.40157216217638236 1103 56-57 1.3125549741688367 1103 58-59 1.0518650945660575 1103 60-61 0.8065230753011399 1103 62-63 1.7287798162185908 1103 64-65 1.520977338380888 1103 66-67 1.052112350984853 1103 68-69 2.0328742332810172 1103 70-71 2.5350200490638777 1103 72-73 2.221305908504821 1103 74-75 2.9710548900071245 1103 76-77 2.8327915350163586 1103 78-79 2.34992295206332 1103 80-81 2.4015007946985065 1103 82-83 3.040167131448978 1103 84-85 3.0710217898892047 1103 86-87 2.336712487919364 1103 88-89 2.6079077636159056 1103 90-91 2.4363161201996846 1103 92-93 2.045627516297156 1103 94-95 1.8764085842578737 1103 96-97 2.108508197579397 1103 98-99 2.5575190838616315 1103 100-101 1.7841331440790213 1104 1 -0.012937836490571897 1104 2 -0.20621931471257682 1104 3 -0.018030703074934706 1104 4 -0.29245953021606397 1104 5 0.04443891443315806 1104 6 -0.2553474467138699 1104 7 -0.10368734538375435 1104 8 -0.0681225145495361 1104 9 -0.2790855258428522 1104 10-11 0.22093872351489097 1104 12-13 -0.1852871355212855 1104 14-15 -0.8614492286536048 1104 16-17 -0.9171599394277976 1104 18-19 -0.8668272041236875 1104 20-21 -1.266256642901034 1104 22-23 -0.6680788591512439 1104 24-25 -0.5162742316281097 1104 26-27 -0.4658732456425767 1104 28-29 -1.7320631620096663 1104 30-31 -1.8641544973780881 1104 32-33 -1.4932747089492366 1104 34-35 -2.0082325413506155 1104 36-37 -1.378125676845066 1104 38-39 -1.6772377357333355 1104 40-41 -2.2058567837721412 1104 42-43 -2.6624265772481124 1104 44-45 -2.3260925314134866 1104 46-47 -2.4718363434456734 1104 48-49 -2.226681169287037 1104 50-51 -3.3206058535622525 1104 52-53 -3.6825028598926615 1104 54-55 -3.3015528378236176 1104 56-57 -1.8671325258311633 1104 58-59 -1.6746974054339425 1104 60-61 -1.2169144246988601 1104 62-63 -1.0134076837814092 1104 64-65 -2.455585161619112 1104 66-67 -2.369762649015147 1104 68-69 -2.1702507667189828 1104 70-71 -2.2696674509361223 1104 72-73 -1.8958815914951792 1104 74-75 -1.6851951099928755 1104 76-77 -1.4406459649836414 1104 78-79 -1.18913954793668 1104 80-81 -1.3016242053014935 1104 82-83 -1.2723328685510218 1104 84-85 -1.2336657101107953 1104 86-87 -1.100787512080636 1104 88-89 -1.3608422363840944 1104 90-91 -1.4464963798003154 1104 92-93 -0.8606224837028442 1104 94-95 -1.5767164157421263 1104 96-97 -1.860241802420603 1104 98-99 -1.0596684161383685 1104 100-101 -1.2002418559209787 1105 1 -0.19492313060822042 1105 2 -0.07570460883022179 1105 3 -0.1503836442514057 1105 4 -0.14723894198076692 1105 5 0.12440214972727404 1105 6 0.17388049446260112 1105 7 0.011202360498600683 1105 8 0.06882601486223194 1105 9 0.26595123886303185 1105 10-11 0.08261151763254304 1105 12-13 0.19476801153754053 1105 14-15 0.7764184184052212 1105 16-17 0.4008547664545574 1105 18-19 0.4369411782292545 1105 20-21 0.47960732768719794 1105 22-23 0.19910864084875612 1105 24-25 -0.0557962904516387 1105 26-27 0.09984366612213336 1105 28-29 -0.46827639730378223 1105 30-31 0.3743565320336799 1105 32-33 0.4488208792860533 1105 34-35 -0.1778097472329634 1105 36-37 0.165532411390231 1105 38-39 0.8659607936784326 1105 40-41 0.9448785103455108 1105 42-43 1.2920770992224746 1105 44-45 1.0121427627041584 1105 46-47 0.6770607153778556 1105 48-49 -0.435320875169392 1105 50-51 -0.5480874712093105 1105 52-53 -1.0864550657750165 1105 54-55 -0.5612035731177336 1105 56-57 -0.8703494375958698 1105 58-59 -1.2905062289633555 1105 60-61 -1.7486239835223891 1105 62-63 -1.4169003308402317 1105 64-65 -1.001541043972054 1105 66-67 -0.3716008843092631 1105 68-69 -1.0360595902483958 1105 70-71 -0.3202189215243578 1105 72-73 -0.3604955620834147 1105 74-75 -0.18059952175758198 1105 76-77 -0.2977231708659964 1105 78-79 0.28236780500449754 1105 80-81 0.296261824110271 1105 82-83 -0.03382183913925729 1105 84-85 -0.3825627689343243 1105 86-87 -0.06310368855122306 1105 88-89 -0.11589738344292044 1105 90-91 -1.0416250562709024 1105 92-93 -1.5076813072322572 1105 94-95 -1.8680767098597748 1105 96-97 -0.8386425377147191 1105 98-99 -1.1079220926089555 1105 100-101 -0.44656538533274315 1106 1 0.09879161556422389 1106 2 -0.10048301334271059 1106 3 0.047037790075748376 1106 4 0.20368773005790786 1106 5 0.16751254457014397 1106 6 0.42508919712174986 1106 7 0.4966979285888513 1106 8 0.29767371832717515 1106 9 0.2262655015544084 1106 10-11 0.5560200591313276 1106 12-13 0.18318461105405248 1106 14-15 0.09360214120940924 1106 16-17 0.36751471810644887 1106 18-19 0.24415310409549562 1106 20-21 0.7267870214825294 1106 22-23 0.4080127504377984 1106 24-25 0.6937000834403833 1106 26-27 0.6427526105218035 1106 28-29 1.0622433448396507 1106 30-31 1.0415603998821865 1106 32-33 1.0223502910507634 1106 34-35 0.9171741367315747 1106 36-37 1.3880344258946593 1106 38-39 2.0639865793351575 1106 40-41 1.2470541751319715 1106 42-43 0.9322866076833947 1106 44-45 1.2903458247508937 1106 46-47 1.1844136565543266 1106 48-49 0.7606904060554278 1106 50-51 0.9646039067117194 1106 52-53 0.9023644346278878 1106 54-55 0.6610969909435056 1106 56-57 0.4069470974565057 1106 58-59 0.36340190963455044 1106 60-61 -0.4820043219591348 1106 62-63 -0.2871662454252437 1106 64-65 0.014877167148011239 1106 66-67 -0.7725879914809006 1106 68-69 -1.4009870680888454 1106 70-71 -0.848220533127904 1106 72-73 -0.40733279012531654 1106 74-75 -0.8057002469791783 1106 76-77 -0.4510269581343245 1106 78-79 -0.8327611232791448 1106 80-81 -1.4252329381782047 1106 82-83 -1.3357960534825288 1106 84-85 -1.3299842032614784 1106 86-87 -0.7786556627655656 1106 88-89 -1.2241769966580662 1106 90-91 -0.5671085373345619 1106 92-93 -0.8366498809631189 1106 94-95 -1.3620331965640418 1106 96-97 -1.0170269051603285 1106 98-99 -0.6573781764123403 1106 100-101 -0.27644048605796456 1107 1 0.010959222332957097 1107 2 0.08606009705212614 1107 3 0.15843988516036234 1107 4 0.22040811684276207 1107 5 0.15381391443315806 1107 6 -0.046707740831514855 1107 7 0.24649647814565867 1107 8 0.21588483839163786 1107 9 0.4424218270983218 1107 10-11 -0.19680024707334098 1107 12-13 -0.0993496355212855 1107 14-15 0.2984772419346271 1107 16-17 0.3493841782192604 1107 18-19 0.6648823546998415 1107 20-21 0.6046073276871979 1107 22-23 0.721167464378162 1107 24-25 0.21626253307777432 1107 26-27 0.32043190141624933 1107 28-29 0.5243706615197468 1107 30-31 -0.16240817384868222 1107 32-33 -0.24235559130217865 1107 34-35 -0.23663327664473144 1107 36-37 -0.23887935331566013 1107 38-39 0.14537255838431662 1107 40-41 0.1581138044631487 1107 42-43 -0.37704054783634433 1107 44-45 -0.9069748843546606 1107 46-47 -0.8082334022692024 1107 48-49 -1.29561499281645 1107 50-51 -1.9230874712093105 1107 52-53 -2.0570433010691325 1107 54-55 -2.3847329848824437 1107 56-57 -3.3041729670076343 1107 58-59 -2.6140356407280585 1107 60-61 -3.9471533952870956 1107 62-63 -4.336017977899058 1107 64-65 -4.148599867501467 1107 66-67 -3.533365590191618 1107 68-69 -4.308118413777809 1107 70-71 -3.8569836274067093 1107 72-73 -4.184024973848121 1107 74-75 -3.651187757051698 1107 76-77 -3.8050761120424674 1107 78-79 -3.541161606760209 1107 80-81 -3.4905028817720805 1107 82-83 -3.379410074433377 1107 84-85 -3.0590333571696178 1107 86-87 -3.29104486502181 1107 88-89 -2.8070738540311524 1107 90-91 -3.4754485856826705 1107 92-93 -3.33121071899696 1107 94-95 -3.221017886330362 1107 96-97 -2.8974660671264854 1107 98-99 -2.460863269079546 1107 100-101 -2.0053889147445076 1108 1 0.43997883017609496 1108 2 0.33586401862075377 1108 3 0.18530263025839844 1108 4 0.10962380311726605 1108 5 0.14048058109982264 1108 6 0.21486088661946212 1108 7 0.35277098794957595 1108 8 0.3300024854504642 1108 9 0.3132061408238158 1108 10-11 0.014376223514886988 1108 12-13 0.5789836978120491 1108 14-15 0.25396743801306343 1108 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2.0930067899028018 2316 64-65 1.801322733117729 2316 66-67 1.610829456248009 2316 68-69 1.6886308122283857 2316 70-71 1.7130628122217715 2316 72-73 0.8662072242942962 2316 74-75 0.44029831105975603 2316 76-77 0.46404153501636003 2316 78-79 0.34885387311595295 2316 80-81 0.8571751368037681 2316 82-83 1.2889335788173995 2316 84-85 1.6377158688365725 2316 86-87 1.142469066866731 2316 88-89 0.9874307899316932 2316 90-91 0.9705266465154736 2316 92-93 0.28937751629715436 2316 94-95 0.41489542636313814 2316 96-97 0.37799832915834486 2316 98-99 0.14370329438794727 2316 100-101 0.9477844598684939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 3.0 6 31.0 7 55.0 8 89.0 9 174.0 10 314.0 11 474.0 12 718.0 13 1045.0 14 1163.0 15 1586.0 16 1989.0 17 2702.0 18 2729.0 19 1865.0 20 1845.0 21 1907.0 22 1860.0 23 1720.0 24 1603.0 25 1552.0 26 1417.0 27 1209.0 28 1230.0 29 1428.0 30 1625.0 31 1898.0 32 2184.0 33 2665.0 34 3648.0 35 6473.0 36 7441.0 37 10879.0 38 5986.0 39 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.76516985340115 20.930453939674166 15.334403133244487 11.969973073680203 2 15.04242457101033 28.382444631190868 33.653663693784054 22.92146710401475 3 27.727777928379734 30.78153379056087 31.516170132017674 9.97451814904172 4 11.567133833934236 21.439995662663666 35.6501938247174 31.342676678684704 5 8.071511832795684 45.082544932093576 31.014665618477053 15.831277616633685 6 21.911680988912686 38.95199110846052 14.181734392366288 24.954593510260512 7 17.49708585215105 42.75122665293177 20.529155032665564 19.22253246225162 8 25.43441134213451 26.793895199110846 18.611239122773725 29.16045433598092 9 21.629754127246606 23.302339450784785 24.13998753015804 30.92791889181057 10-11 24.266041367345277 28.86903955108569 21.09436417360189 25.770554907967146 12-13 31.942448968527202 28.620998129523706 23.660169698284044 15.776383203665048 14-15 25.242284180706726 29.47327757973352 31.524052211393794 13.76038602816596 16-17 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33.40269352510184 37.09527650316188 15.874446756450835 42-43 13.539379151416565 33.41331164429985 34.37034024671276 18.67696895757083 44-45 15.290880503144654 29.86540338321405 35.40311212318369 19.4406039904576 46-47 18.255689442509183 32.59213576046736 34.85164753242881 14.300527264594656 48-49 16.52863997397598 30.880479275664836 35.383854265499195 17.207026484859984 50-51 11.638970966954918 32.726558052535985 38.62533546585703 17.009135514652066 52-53 14.952289300333433 34.37881211201171 32.3361977825368 18.33270080511806 54-55 12.803952397733742 30.462332944780286 33.95050014909594 22.783214508390035 56-57 17.453034780015724 29.59079942530294 31.56835370977798 21.38781208490336 58-59 19.819319159895088 29.832670294741547 31.782478160389825 18.565532384973533 60-61 15.892993906861049 32.404112699348666 34.228665541570926 17.474227852219354 62-63 13.369118055696752 32.71116713769663 33.64306094924398 20.27665385736264 64-65 12.99049839380837 35.98885831627744 30.862599455114736 20.158043834799464 66-67 15.554832631871445 31.040535686449928 29.11642911846234 24.288202563216288 68-69 13.848958651024407 30.7233529200469 31.54409721516242 23.883591213766277 70-71 18.163273604597578 29.857138984521132 29.75751579061509 22.222071620266203 72-73 16.308384613299356 26.43267640760118 35.13852367914554 22.120415299953915 74-75 13.789340995960856 32.14915015316219 30.931307435820976 23.13020141505598 76-77 15.048286540890754 27.265935916765144 32.00426497154422 25.681512570799885 78-79 15.48090216595733 27.97039767952506 33.27346905581609 23.27523109870151 80-81 17.21427022337888 27.027759705053136 31.689844936022553 24.068125135545433 82-83 14.8560546504378 26.415055978747056 34.77730488763588 23.951584483179268 84-85 14.947545338718859 28.496977418742713 32.123397218683074 24.43208002385535 86-87 14.87317943464959 29.306099190709094 30.78018899282967 25.04053238181164 88-89 14.491728341314758 29.254190248149758 28.265128428384852 27.988952982150632 90-91 16.150597452411233 26.594623268340122 28.915744588244095 28.33903469100455 92-93 17.4164385047033 29.072352191710266 28.982894629835453 24.528314673750984 94-95 16.1830084849142 27.746076066035947 29.375288026240888 26.695627422808965 96-97 12.793786765702514 28.793813874054596 31.35352001951801 27.05887934072488 98-99 15.797606300066416 29.896851322227796 26.435067839570596 27.87047453813519 100-101 14.904272982955508 27.579546609738742 28.044457998712346 29.471722408593404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1312.0 1 803.5 2 349.0 3 384.5 4 288.0 5 263.0 6 431.5 7 715.5 8 963.5 9 999.0 10 756.5 11 505.5 12 445.0 13 436.0 14 424.0 15 421.0 16 416.0 17 411.0 18 453.0 19 511.5 20 548.5 21 590.5 22 669.5 23 756.0 24 809.5 25 890.5 26 1050.5 27 1173.5 28 1265.0 29 1401.0 30 1500.5 31 1501.0 32 1478.5 33 1467.5 34 1420.5 35 1333.5 36 1221.0 37 1065.0 38 896.5 39 771.0 40 692.0 41 658.0 42 641.0 43 644.5 44 642.0 45 720.0 46 1370.5 47 4079.5 48 8720.0 49 8875.0 50 5437.5 51 3294.0 52 1557.0 53 650.5 54 375.5 55 317.5 56 299.0 57 281.5 58 245.5 59 206.0 60 203.5 61 185.5 62 159.5 63 141.5 64 132.0 65 119.0 66 117.5 67 123.5 68 101.5 69 74.5 70 64.0 71 51.5 72 40.5 73 39.5 74 26.0 75 10.5 76 11.0 77 9.0 78 5.0 79 4.5 80 3.5 81 2.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3307218954159777 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001355417604163843 16-17 0.001355417604163843 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.777088020819215E-4 26-27 6.777088020819215E-4 28-29 0.0 30-31 0.002033126406245764 32-33 0.0 34-35 0.008132505624983057 36-37 0.023719808072867252 38-39 0.023042099270785328 40-41 0.011521049635392664 42-43 0.010843340833310743 44-45 0.002710835208327686 46-47 0.001355417604163843 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.002033126406245764 60-61 0.008810214427064979 62-63 0.00474396161457345 64-65 0.001355417604163843 66-67 0.00474396161457345 68-69 0.00474396161457345 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21008972864539566 78-79 0.0 80-81 0.002710835208327686 82-83 0.0 84-85 0.0 86-87 0.09691235869771476 88-89 0.37138442354089296 90-91 0.34902003307218954 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001355417604163843 100-101 6.777088020819215E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 73778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.63363062159451 #Duplication Level Percentage of deduplicated Percentage of total 1 84.21649968691295 29.167231424001734 2 7.948497182216656 5.505706308113529 3 2.774733876017533 2.882973244056494 4 1.3267063243581716 1.837946271246171 5 0.8649029430181591 1.4977364526010464 6 0.5087664370695053 1.0572257312477973 7 0.34439574201628054 0.8349372441649272 8 0.23090169067000627 0.6397571091653338 9 0.28177833437695676 0.8783106074981702 >10 1.1505948653725735 7.626934858629944 >50 0.13697557921102066 3.206918051451652 >100 0.12914840325610522 10.379788012686708 >500 0.058703819661866 14.8485998536149 >1k 0.02739511584220413 19.635934831521592 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3225 4.371221773428393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 3.154056764889263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 2.8260457046816123 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2008 2.7216785491609965 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1995 2.7040581203068665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 2.069722681558188 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1320 1.7891512374962726 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 975 1.3215321640597468 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 971 1.3161104936430914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 910 1.233430019789097 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 847 1.1480387107267749 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 827 1.120930358643498 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 761 1.0314727967686845 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 745 1.009786115102063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 714 0.9677681693729838 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 708 0.9596356637480008 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 683 0.9257502236439046 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 599 0.8118951448941418 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 597 0.8091843096858142 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 580 0.7861422104150289 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 537 0.7278592534359837 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 501 0.6790642196860852 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 488 0.6614437908319554 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.6411125267694977 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 467 0.6329800211445146 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 452 0.6126487570820569 RNA PCR Primer, Index 33 (95% over 22bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 359 0.4865949198948196 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.4865949198948196 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 330 0.44728780937406815 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 321 0.43508905093659356 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.41204695166580824 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 275 0.37273984114505676 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.3483423242701076 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 255 0.34563148906177993 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 252 0.3415652362492884 RNA PCR Primer, Index 33 (96% over 29bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 241 0.3266556426034861 RNA PCR Primer, Index 33 (95% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 241 0.3266556426034861 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 228 0.30903521374935616 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 183 0.24804142156198325 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.24804142156198325 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 177 0.2399089159370002 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 169 0.22906557510368944 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 154 0.2087343110412318 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 149 0.2019572230204126 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 145 0.1965355526037572 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 143 0.19382471739542953 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 137 0.18569221177044648 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 127 0.17213803572880804 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 125 0.16942720052048035 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 125 0.16942720052048035 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 116 0.1572284420830058 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 110 0.14909593645802272 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 107 0.1450296836455312 RNA PCR Primer, Index 33 (96% over 27bp) TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 104 0.14096343083303967 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 102 0.13825259562471198 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.12740925479140122 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 90 0.12198758437474587 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 88 0.11927674916641817 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 84 0.11385507874976279 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 84 0.11385507874976279 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 80 0.10843340833310744 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 79 0.10707799072894358 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.10301173791645206 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.777088020819215E-4 0.0 14-15 0.0 0.0 0.0 0.001355417604163843 0.0 16-17 0.0 0.0 0.0 0.001355417604163843 0.0 18-19 0.0 0.0 0.0 0.003388544010409607 0.0 20-21 0.0 0.0 0.0 0.006099379218737292 0.0 22-23 0.0 0.0 0.0 0.022364390468703407 0.0 24-25 0.0 0.0 0.0 0.05286128656238987 0.0 26-27 0.0 0.0 0.0 0.07929192984358481 0.0 28-29 0.0 0.0 0.0 0.19992409661416682 0.0 30-31 0.0 0.0 0.0 0.414080078072054 0.0 32-33 0.0 0.0 0.0 0.7454796822901135 0.0 34-35 0.0 0.0 0.0 1.2029331236954106 0.0 36-37 0.0 0.0 0.0 1.7633983030171596 0.0 38-39 0.0 0.0 0.0 2.426197511453279 0.0 40-41 0.0 0.0 0.0 3.0686654558269404 0.0 42-43 0.0 0.0 0.0 3.621675838325788 0.0 44-45 0.0 0.0 0.0 4.1706199680121445 0.0 46-47 0.0 0.0 0.0 4.739217652958876 0.0 48-49 0.0 0.0 0.0 5.254276342541138 0.0 50-51 0.0 0.0 0.0 5.706985822331861 0.0 52-53 0.0 0.0 0.0 6.130553823633061 0.0 54-55 0.0 0.0 0.0 6.671365447694434 0.0 56-57 0.0 0.0 0.0 7.351785084984684 0.0 58-59 0.0 0.0 0.0 7.861422104150289 0.0 60-61 0.0 0.0 0.0 8.378513920138793 0.0 62-63 0.0 0.0 0.0 8.873241345658597 0.0 64-65 0.0 0.0 0.0 9.353059177532597 0.0 66-67 0.0 0.0 0.0 9.916912900864757 0.0 68-69 0.0 0.0 0.0 10.606820461384153 0.0 70-71 0.0 0.0 0.0 11.325869500393072 0.0 72-73 0.0 0.0 0.0 11.920220119818916 0.0 74-75 0.0 0.0 0.0 12.631814362004935 0.0 76-77 0.0 0.0 0.0 13.223454146222451 0.0 78-79 0.0 0.0 0.0 14.2000325300225 0.0 80-81 0.0 0.0 0.0 15.00718371330207 0.0 82-83 0.0 0.0 0.0 15.654395619290304 0.0 84-85 0.0 0.0 0.0 16.418851148038712 0.0 86-87 0.0 0.0 0.0 17.132478516630975 0.0 88-89 0.0 0.0 0.0 17.976903684025046 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGAAA 35 3.4032546E-6 67.7971 6 ATGGGCA 30 1.2988785E-4 63.277283 5 TGGGCAG 45 9.6082775E-4 42.184856 6 GGGCAGT 55 0.0025752245 34.514885 7 ATGGGGT 75 3.0666843E-4 31.638643 5 ATGGGGA 95 3.4681023E-5 29.973454 5 TGGGGAA 80 4.4765827E-4 29.66123 6 GGCAGTG 65 0.0058343266 29.204903 8 CATGGGG 230 0.0 28.887457 4 GTACTTT 1490 0.0 21.673365 14-15 GAGTACT 1610 0.0 21.532806 12-13 ACTTTTT 1580 0.0 20.582106 16-17 CATGGGC 210 8.4573E-7 20.339128 4 GTACATG 1105 0.0 19.83695 1 AGTACTT 1390 0.0 19.474384 12-13 TACATGG 1085 0.0 19.245626 2 ACATGGG 1095 0.0 18.203056 3 AGTACTC 235 8.986917E-7 18.17539 7 TACTTTT 1530 0.0 17.692413 14-15 CTTGAAA 320 0.0 15.582709 56-57 >>END_MODULE