##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939226_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72890 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.080916449444366 33.0 31.0 34.0 30.0 34.0 2 32.23524488955961 33.0 31.0 34.0 30.0 34.0 3 32.27069556866511 34.0 31.0 34.0 30.0 34.0 4 35.82099053368089 37.0 35.0 37.0 35.0 37.0 5 35.821772533955276 37.0 35.0 37.0 35.0 37.0 6 35.983303608176705 37.0 35.0 37.0 35.0 37.0 7 35.924845657840585 37.0 35.0 37.0 35.0 37.0 8 35.975209219371656 37.0 35.0 37.0 35.0 37.0 9 37.504637124434076 39.0 37.0 39.0 35.0 39.0 10-11 37.449403210316916 39.0 37.0 39.0 34.5 39.0 12-13 37.394635752503774 39.0 37.0 39.0 34.0 39.0 14-15 38.368219234462885 40.0 38.0 41.0 33.5 41.0 16-17 38.53011387021539 40.0 38.0 41.0 34.5 41.0 18-19 38.66224447798052 40.0 38.0 41.0 34.0 41.0 20-21 38.67163534092468 40.0 38.0 41.0 34.5 41.0 22-23 38.53065578268624 40.0 38.0 41.0 34.5 41.0 24-25 38.31385649609 40.0 37.0 41.0 34.0 41.0 26-27 37.91615447935244 40.0 36.5 41.0 33.5 41.0 28-29 37.603326930991905 40.0 35.0 41.0 33.0 41.0 30-31 37.086157223213064 39.0 35.0 41.0 33.0 41.0 32-33 36.29488955961037 38.0 35.0 41.0 31.5 41.0 34-35 35.79330498010701 38.0 35.0 40.0 31.0 41.0 36-37 35.286404170668135 37.5 35.0 40.0 30.0 41.0 38-39 34.82202634106187 37.0 35.0 40.0 28.0 41.0 40-41 34.463108794073264 37.0 35.0 40.0 25.0 41.0 42-43 33.8080120729867 36.5 34.5 40.0 21.0 41.0 44-45 33.0368363287145 36.0 33.0 40.0 8.5 41.0 46-47 32.019282480449995 35.0 33.0 40.0 2.0 41.0 48-49 31.037611469337357 35.0 31.5 40.0 2.0 41.0 50-51 30.31735491837015 35.0 31.0 40.0 2.0 41.0 52-53 29.828028536150363 35.0 29.0 40.0 2.0 41.0 54-55 29.37601865825216 35.0 27.5 40.0 2.0 41.0 56-57 28.691576347921526 35.0 24.5 39.5 2.0 41.0 58-59 27.949869666620934 35.0 20.0 39.0 2.0 41.0 60-61 27.061092056523528 35.0 5.0 39.0 2.0 40.0 62-63 26.383941555768967 35.0 2.0 38.0 2.0 40.0 64-65 25.60972012621759 34.5 2.0 37.5 2.0 39.5 66-67 24.762587460557004 34.0 2.0 37.0 2.0 39.0 68-69 23.979160378652765 33.0 2.0 36.0 2.0 39.0 70-71 23.203093702839894 32.5 2.0 35.0 2.0 38.0 72-73 22.5463506653862 31.5 2.0 35.0 2.0 37.0 74-75 22.08636301275895 31.0 2.0 35.0 2.0 37.0 76-77 20.55550144052682 28.5 2.0 33.5 2.0 35.0 78-79 21.196474139113732 30.5 2.0 34.0 2.0 35.0 80-81 21.085594731787623 30.5 2.0 35.0 2.0 35.0 82-83 20.71500891754699 30.0 2.0 34.5 2.0 35.0 84-85 20.30274386061188 29.0 2.0 34.0 2.0 35.0 86-87 19.886459047880365 29.0 2.0 34.0 2.0 35.0 88-89 19.28387295925367 27.5 2.0 34.0 2.0 35.0 90-91 18.771820551515983 25.5 2.0 34.0 2.0 35.0 92-93 18.423974482096312 25.0 2.0 34.0 2.0 35.0 94-95 17.956509809301686 24.0 2.0 33.5 2.0 35.0 96-97 17.286143503910004 19.5 2.0 33.0 2.0 35.0 98-99 16.46867883111538 2.0 2.0 33.0 2.0 35.0 100-101 12.551275895184524 2.0 2.0 27.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4786583322181599 1101 2 0.16360636823581132 1101 3 0.3851518351917562 1101 4 0.2260356171396225 1101 5 0.11357240174334748 1101 6 0.39563126908044666 1101 7 0.46716206285709916 1101 8 0.07277025317730335 1101 9 0.24639126909976028 1101 10-11 0.3864441686334956 1101 12-13 -0.012836021634178962 1101 14-15 0.13666380048668003 1101 16-17 0.6892684396820528 1101 18-19 0.09702721827957816 1101 20-21 0.4788585506499814 1101 22-23 0.6653544482516693 1101 24-25 0.985837954198459 1101 26-27 0.9891675005261362 1101 28-29 1.090853401103736 1101 30-31 1.1772297872638262 1101 32-33 1.4390109196770382 1101 34-35 1.4923067347184684 1101 36-37 1.4164604753507533 1101 38-39 1.3084121549925953 1101 40-41 2.306631007784425 1101 42-43 2.2520781186779146 1101 44-45 3.289464401777842 1101 46-47 2.6644747211631845 1101 48-49 2.8598922386413683 1101 50-51 3.033671813694454 1101 52-53 2.6635030884090583 1101 54-55 1.6797368720185624 1101 56-57 1.9345910136827982 1101 58-59 2.5344689256022868 1101 60-61 3.0654296147205464 1101 62-63 3.093812447137527 1101 64-65 2.556576573773082 1101 66-67 3.1497670194710103 1101 68-69 3.5800621757617463 1101 70-71 2.9017972682857085 1101 72-73 2.632354868527237 1101 74-75 1.2494124879134603 1101 76-77 1.163473343032301 1101 78-79 2.10690441386458 1101 80-81 1.2802324177011712 1101 82-83 1.3831763238182475 1101 84-85 1.4838173064308613 1101 86-87 1.987442763331618 1101 88-89 2.639754972993771 1101 90-91 1.6917078786638662 1101 92-93 1.269131085494596 1101 94-95 2.3478254753714793 1101 96-97 2.519442459203219 1101 98-99 0.5626340455504639 1101 100-101 0.8791666365913553 1102 1 0.6212812830378383 1102 2 0.5201637452849894 1102 3 0.16247423956333762 1102 4 0.4593689504729568 1102 5 0.4624795055684814 1102 6 0.48552197946295905 1102 7 0.6403861065729473 1102 8 0.5063768105543573 1102 9 0.6748065696462078 1102 10-11 0.6006518189067194 1102 12-13 0.35301097290133754 1102 14-15 0.7704069698855847 1102 16-17 0.31809357629407486 1102 18-19 0.26888514177684897 1102 20-21 0.2988039058412326 1102 22-23 0.393906360819976 1102 24-25 0.5243625443623969 1102 26-27 0.08725493222012659 1102 28-29 -0.13947446774871963 1102 30-31 0.08979809327475863 1102 32-33 -0.6743770584650406 1102 34-35 -0.7130211341339958 1102 36-37 -0.38176356836509484 1102 38-39 0.07753783805269876 1102 40-41 0.46086598046201743 1102 42-43 1.2528977908090582 1102 44-45 1.0461583908488805 1102 46-47 1.500267070889958 1102 48-49 1.967405899843559 1102 50-51 1.7149559666999181 1102 52-53 1.3626834162779105 1102 54-55 1.6961303146415112 1102 56-57 2.6515309043931836 1102 58-59 2.3396601824328904 1102 60-61 2.7905662267424027 1102 62-63 2.291080206700368 1102 64-65 1.6733798524616077 1102 66-67 0.8802315003453209 1102 68-69 0.8561550719366124 1102 70-71 0.23663333385947993 1102 72-73 -0.07461234458751775 1102 74-75 0.1787840726129133 1102 76-77 -0.15865780450868172 1102 78-79 -0.7048442200152003 1102 80-81 0.08610673464106 1102 82-83 0.3942418975887385 1102 84-85 0.13955501134889303 1102 86-87 0.5360766431130379 1102 88-89 0.188798688840766 1102 90-91 0.5329647092649594 1102 92-93 1.2144862767514297 1102 94-95 1.0601205573386956 1102 96-97 1.3153440985474774 1102 98-99 1.8894100018346194 1102 100-101 2.0041666365913553 1103 1 -0.28839613631700445 1103 2 0.3185508420591816 1103 3 0.407097895477321 1103 4 -0.1933192215700572 1103 5 0.22753326900934212 1103 6 -0.07845651516069552 1103 7 0.20275169797079684 1103 8 -0.12104254428435723 1103 9 0.6543764621193233 1103 10-11 0.13075934578844084 1103 12-13 0.22989269333144335 1103 14-15 -0.202442492480003 1103 16-17 0.8307279848962281 1103 18-19 0.592003421346746 1103 20-21 -0.040443405986728465 1103 22-23 -0.007437725201526746 1103 24-25 -0.20466971370211695 1103 26-27 0.37005063114485637 1103 28-29 -0.3959260806519538 1103 30-31 0.5521636846725997 1103 32-33 0.5164831565887198 1103 34-35 1.3845595110273052 1103 36-37 2.309903098301568 1103 38-39 2.6205485907408743 1103 40-41 2.512210066483526 1103 42-43 2.3399945650026126 1103 44-45 2.7991153800961968 1103 46-47 3.323385350459848 1103 48-49 4.102620953606998 1103 50-51 4.5356548914310935 1103 52-53 3.3901027711166165 1103 54-55 4.188065798512479 1103 56-57 5.135939506543721 1103 58-59 4.529445128669455 1103 60-61 3.5733619256671325 1103 62-63 3.660972679818652 1103 64-65 4.177412110526124 1103 66-67 3.152543328302311 1103 68-69 3.562875502044136 1103 70-71 3.972923656440127 1103 72-73 3.8971618489608666 1103 74-75 2.9629238575591508 1103 76-77 2.757471227749381 1103 78-79 2.6666611563288853 1103 80-81 3.250354046469017 1103 82-83 3.2998870588790616 1103 84-85 2.671813075865021 1103 86-87 3.012958363543145 1103 88-89 3.205196538303131 1103 90-91 3.3211367522757094 1103 92-93 2.531690577826698 1103 94-95 2.997217331532241 1103 96-97 4.01534409854748 1103 98-99 3.278657313662574 1103 100-101 2.7823924430429674 1104 1 0.027341889098444483 1104 2 -0.13892716380591708 1104 3 0.367019694108798 1104 4 0.5896719807759894 1104 5 0.33066132375029866 1104 6 0.25976440370538256 1104 7 0.5888709550577929 1104 8 0.34425559843314346 1104 9 0.2990489938886327 1104 10-11 0.13247000072490067 1104 12-13 0.36967763956801036 1104 14-15 0.3166190910977065 1104 16-17 0.6794572126577094 1104 18-19 0.6870669599586705 1104 20-21 0.388197845235176 1104 22-23 0.25526999718361765 1104 24-25 -0.022607152607299952 1104 26-27 0.2630125079776988 1104 28-29 -0.08947446774872247 1104 30-31 0.21858597206263397 1104 32-33 -0.35619524028322047 1104 34-35 0.024100077987220914 1104 36-37 0.18869097708944338 1104 38-39 0.25178026229512795 1104 40-41 0.5752599198559594 1104 42-43 0.4377462756575454 1104 44-45 -0.23338706369657558 1104 46-47 0.5472367678596548 1104 48-49 1.0454362028738622 1104 50-51 0.5429862697302177 1104 52-53 0.7581379617324586 1104 54-55 0.8476454661566635 1104 56-57 0.0015309043931850397 1104 58-59 -0.7012489084762024 1104 60-61 -0.04882771265153707 1104 62-63 -0.09073797511781478 1104 64-65 -0.8152565111747592 1104 66-67 -1.2462836511698292 1104 68-69 -1.5536934129118762 1104 70-71 -1.6353363631102162 1104 72-73 -1.4571881021632755 1104 74-75 -1.7401553213264798 1104 76-77 -0.8495668954177731 1104 78-79 -0.6199957351667145 1104 80-81 -0.7237417502074237 1104 82-83 -0.7277277993809577 1104 84-85 -0.9968086250147437 1104 86-87 -0.9381657811293884 1104 88-89 -0.8331710081289323 1104 90-91 -0.38218680588655474 1104 92-93 -1.4370288747637225 1104 94-95 -1.329273382055245 1104 96-97 -1.189201355997973 1104 98-99 -0.4227112102865931 1104 100-101 0.5988636062883241 1105 1 -0.678718716962166 1105 2 -0.15291317779193037 1105 3 0.34580757289667474 1105 4 0.0721894632934692 1105 5 -0.20546921238023685 1105 6 0.017573261514243654 1105 7 -0.31089594470910953 1105 8 0.3025306567081998 1105 9 -0.6739113790717397 1105 10-11 0.33398515224005365 1105 12-13 0.38249815238852136 1105 14-15 0.23515055962917586 1105 16-17 0.24437562757611886 1105 18-19 -0.46829434540264003 1105 20-21 -0.6486319915946623 1105 22-23 -0.34776030584668405 1105 24-25 -0.18717591717606297 1105 26-27 -0.6730014780362836 1105 28-29 -0.4817821600564187 1105 30-31 -0.4230224195457595 1105 32-33 -0.45771039179837913 1105 34-35 -0.04955959567244861 1105 36-37 -0.4516353632368961 1105 38-39 -0.16220575169089102 1105 40-41 0.06791726251330488 1105 42-43 0.15674394465521857 1105 44-45 0.5339789036694 1105 46-47 0.42462604524893166 1105 48-49 1.0859956434332965 1105 50-51 0.8027764795204249 1105 52-53 0.4742218778163725 1105 54-55 0.2961303146415126 1105 56-57 -0.027956275093998784 1105 58-59 0.5768396696123794 1105 60-61 0.7803098164859925 1105 62-63 0.7962084118285766 1105 64-65 1.0945336986154537 1105 66-67 1.2065135516273777 1105 68-69 0.4452576360391731 1105 70-71 0.19624871847486602 1105 72-73 0.9119261169509443 1105 74-75 0.5332712521000929 1105 76-77 -0.22404241989329776 1105 78-79 0.7425916774206947 1105 80-81 0.7893118628461906 1105 82-83 1.0884726668195057 1105 84-85 1.4318627036565879 1105 86-87 1.146333053369446 1105 88-89 1.2817474067894814 1105 90-91 1.170144196444447 1105 92-93 0.46320422546937934 1105 94-95 0.356274403492538 1105 96-97 0.08842102162440568 1105 98-99 -0.05930794688332952 1105 100-101 -0.8054487480240287 1106 1 -0.4463657757856936 1106 2 -0.4945421370679526 1106 3 -0.1659571329856817 1106 4 0.0936826759631515 1106 5 0.07620499576456297 1106 6 0.20807099907079873 1106 7 -0.1321629130348967 1106 8 -0.03185848356329046 1106 9 -0.00264441074595112 1106 10-11 -0.26748543599523344 1106 12-13 -0.6254203996476733 1106 14-15 -0.5423381281536308 1106 16-17 -0.10641622762749137 1106 18-19 -0.28797760332118827 1106 20-21 -0.16639217259014316 1106 22-23 0.1948867529768492 1106 24-25 0.027303720832982492 1106 26-27 0.11127454006326332 1106 28-29 0.4914078851924515 1106 30-31 0.32754319131397125 1106 32-33 0.5584660787898557 1106 34-35 0.5252141599836548 1106 36-37 0.44225603947803904 1106 38-39 0.7785182302095706 1106 40-41 1.045179705952215 1106 42-43 1.827897790809061 1106 44-45 1.748119175162607 1106 46-47 1.5095807963801526 1106 48-49 1.607601978274925 1106 50-51 2.487504986307755 1106 52-53 2.940624592748499 1106 54-55 2.402012667582685 1106 56-57 1.7196681592951428 1106 58-59 1.8038758687074008 1106 60-61 1.755762305173775 1106 62-63 0.55921746160233 1106 64-65 0.5042622054027852 1106 66-67 0.8439569905414039 1106 68-69 2.021841346446415 1106 70-71 2.149868627977124 1106 72-73 0.44744647894189526 1106 74-75 -0.15258847640669515 1106 76-77 0.18251866607955236 1106 78-79 -0.1367069651132411 1106 80-81 0.39836163660184454 1106 82-83 1.2707124858240348 1106 84-85 0.720437364290067 1106 86-87 0.7164687999757824 1106 88-89 1.4471320221740989 1106 90-91 2.297180395539467 1106 92-93 1.812525492437704 1106 94-95 1.1542382043975152 1106 96-97 0.4050499809004222 1106 98-99 0.4487237273248148 1106 100-101 0.048284283650177784 1107 1 -0.2257775404915776 1107 2 0.02016374528498943 1107 3 -0.1512512506327397 1107 4 0.06427091125726747 1107 5 0.09091087811750498 1107 6 0.2816004108355088 1107 7 0.29430767520039325 1107 8 -0.22303495415152952 1107 9 -0.13499735192242213 1107 10-11 0.05604397576946951 1107 12-13 0.3010501885876167 1107 14-15 0.25177951890519523 1107 16-17 -0.1505338746863174 1107 18-19 0.6237871025611668 1107 20-21 0.5615490038804509 1107 22-23 0.3198867529768421 1107 24-25 0.6228919561271056 1107 26-27 0.023039245945618347 1107 28-29 0.020819649898335513 1107 30-31 0.44519025013749314 1107 32-33 0.8378778434957397 1107 34-35 -0.04096231060457711 1107 36-37 0.552550157125097 1107 38-39 0.6314594066801504 1107 40-41 0.030473823599272976 1107 42-43 0.12936837904435095 1107 44-45 -0.40629258954327696 1107 46-47 -0.41688979185514086 1107 48-49 -1.179162727607423 1107 50-51 -0.953671484280477 1107 52-53 -1.015257760192675 1107 54-55 -1.3994579206526048 1107 56-57 -1.2950377230577956 1107 58-59 -1.6961241312925992 1107 60-61 -1.486884753649754 1107 62-63 -1.9040178325153185 1107 64-65 -2.1869142651854503 1107 66-67 -1.053101832988009 1107 68-69 -1.3825704182594691 1107 70-71 -0.7986607837875788 1107 72-73 -0.36872999164634024 1107 74-75 -0.8731767117008111 1107 76-77 -0.45718721627338965 1107 78-79 -0.9455304945250056 1107 80-81 -0.33693248104521345 1107 82-83 0.18247719170638277 1107 84-85 -0.25750381218052 1107 86-87 0.5473511529169564 1107 88-89 0.013308492762334367 1107 90-91 0.488356866127706 1107 92-93 0.5404666689082909 1107 94-95 0.029238204397515233 1107 96-97 -0.2273029602760488 1107 98-99 -0.2350998020869497 1107 100-101 -1.5620098339968802 1108 1 -0.2816172676868014 1108 2 -0.04143045761355779 1108 3 0.2530539497082671 1108 4 -0.1942542379328387 1108 5 -0.357085711822819 1108 6 -0.09926062923269541 1108 7 -0.2885994006734336 1108 8 -0.5233333343731843 1108 9 -0.13968618397697696 1108 10-11 -0.1326815144266149 1108 12-13 -0.14481511405518432 1108 14-15 0.15555189742181597 1108 16-17 0.40178922846799026 1108 18-19 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-3.6987465843199985 2316 66-67 -4.821779993907551 2316 68-69 -4.42804033036224 2316 70-71 -4.17687241326696 2316 72-73 -4.12777326412775 2316 74-75 -4.754261904398579 2316 76-77 -4.96268079301443 2316 78-79 -4.8203614613945085 2316 80-81 -4.5319967136348005 2316 82-83 -4.642827067928501 2316 84-85 -4.79722659784651 2316 86-87 -4.482314161484666 2316 88-89 -4.889074874377625 2316 90-91 -4.93312724475803 2316 92-93 -4.728042458880754 2316 94-95 -4.815166798983146 2316 96-97 -5.102471993406542 2316 98-99 -5.733578503912508 2316 100-101 -4.040373593293703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 6.0 5 15.0 6 34.0 7 62.0 8 87.0 9 178.0 10 345.0 11 496.0 12 785.0 13 1033.0 14 1177.0 15 1612.0 16 2124.0 17 2761.0 18 2574.0 19 1881.0 20 1909.0 21 1948.0 22 1968.0 23 1689.0 24 1684.0 25 1652.0 26 1510.0 27 1264.0 28 1381.0 29 1502.0 30 1766.0 31 1915.0 32 2374.0 33 3010.0 34 4350.0 35 6774.0 36 7925.0 37 9529.0 38 3479.0 39 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.51961661869811 21.02400264400898 15.247118443339714 12.209262293953206 2 15.013033337906434 28.687062697214984 33.363973110166 22.93593085471258 3 27.680065852654685 30.672245849910823 31.555768966936483 10.09191933049801 4 11.43503910001372 22.10454108931266 35.2188228837975 31.241596926876113 5 7.922897516806146 45.1474825078886 31.356839072575116 15.572780902730141 6 21.960488407188915 39.090410207161476 13.730278501852105 25.218822883797504 7 17.232816572918097 42.72053779667993 20.377280834133625 19.669364796268347 8 25.331321168884617 27.210865688023052 18.526546851419948 28.93126629167238 9 21.377418027164218 23.560159143915488 23.984085608451092 31.078337220469198 10-11 24.31266291672383 28.85923995061051 21.210042529839484 25.618054602826177 12-13 31.773905885581012 28.82631362326794 23.584853889422416 15.814926601728633 14-15 24.904650843737137 29.90533680889011 31.214158320757306 13.975854026615448 16-17 15.016978219859373 33.96672954896244 31.17201166180758 19.844280569370607 18-19 14.086294416243655 33.35848538894224 35.65852654685142 16.89669364796268 20-21 18.42022225270956 27.51543421594183 34.04445054191247 20.019892989436137 22-23 15.457538757031141 30.006859651529705 36.47070928796817 18.064892303470984 24-25 20.00205789545891 31.043352997667718 34.10550144052682 14.84908766634655 26-27 17.210084033613445 28.70108043217287 40.45892642771394 13.629909106499744 28-29 13.663053916861024 36.12155302510632 36.094114418987516 14.121278639045137 30-31 15.624914254355879 31.79997256139388 35.40883523117026 17.166277953079984 32-33 15.127589518452464 32.99355192756208 37.63204829194677 14.24681026203869 34-35 16.80421494576962 30.827965177303508 36.302455288233965 16.065364588692916 36-37 14.44901095734389 31.178686353749992 39.908883201712555 14.46341948719356 38-39 14.821345261944618 32.02681193227037 38.09928922308516 15.052553582699854 40-41 13.5385438604916 33.54005309770939 37.17114064032819 15.750262401470822 42-43 13.719464090937155 33.668562334071936 34.04723847678894 18.56473509820197 44-45 15.31000041159603 30.313361779192448 35.375992975427714 19.000644833783802 46-47 18.339473016265014 32.803742805595 34.715619490577815 14.141164687562169 48-49 16.650088834315426 31.08995246163661 34.94309801957785 17.316860684470115 50-51 11.581229120792432 32.80582251215882 38.56659738371096 17.046350983337792 52-53 14.950610508986143 34.52462614899163 32.038002469474556 18.486760872547674 54-55 12.717107970915078 30.732610783372206 33.65276443956647 22.89751680614625 56-57 17.314446426121552 29.49375771710797 31.67238304294142 21.51941281382906 58-59 19.67992426840813 29.99492378822593 31.71329007120416 18.611861872161782 60-61 15.784382995945862 32.40634668020333 34.161767631382176 17.647502692468635 62-63 13.210407534692925 32.69332345536738 33.30109274998457 20.795176259955138 64-65 12.876007545875492 36.05968101526325 31.01354827645344 20.05076316240782 66-67 15.549305436460298 31.062939461498885 28.926084719602123 24.46167038243869 68-69 13.599226194151182 30.972127897484448 31.357658825708462 24.070987082655908 70-71 18.11714993243197 29.96522132817484 29.5399200159145 22.37770872347869 72-73 16.300589930031553 26.535875977500346 35.08574564412128 22.077788448346823 74-75 13.916861023460008 32.228700782000274 30.489093154067774 23.365345040471944 76-77 14.969110378912687 27.49176276771005 31.624794069192752 25.914332784184513 78-79 15.506678649588714 28.0503282726069 33.07560903658679 23.367384041217594 80-81 17.144444673101063 27.02789839414456 31.549811701273846 24.27784523148053 82-83 14.744134997942105 26.514611057758263 34.39909452599808 24.342159418301552 84-85 14.893675401289613 28.695980244203596 31.697077788448347 24.713266566058444 86-87 14.728421341756507 29.2906681315663 30.47655016136785 25.50436036530935 88-89 14.37385944978136 28.946045518713632 27.723031367282996 28.957063664222016 90-91 15.997906610016596 26.44213222787652 28.868123317196787 28.6918378449101 92-93 17.556592125120044 28.747427630676363 28.786527644395665 24.90945259980793 94-95 16.177802167649883 27.660172863218545 28.97311016600357 27.188914803128 96-97 12.726025517903691 28.740567979146658 30.95760735354644 27.575799149403213 98-99 15.682642904670768 29.819795718176145 26.225999629576275 28.27156174757681 100-101 15.349156262861847 27.23830429414186 27.526409658389355 29.88612978460694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1262.0 1 798.5 2 361.5 3 385.5 4 296.0 5 231.5 6 415.0 7 702.5 8 873.0 9 931.0 10 727.5 11 464.5 12 405.5 13 408.0 14 422.5 15 408.0 16 409.5 17 420.0 18 409.0 19 453.5 20 523.0 21 600.0 22 666.5 23 713.5 24 811.0 25 939.5 26 1034.0 27 1139.5 28 1300.5 29 1398.0 30 1433.0 31 1519.0 32 1573.0 33 1526.0 34 1443.0 35 1357.0 36 1246.5 37 1088.0 38 916.5 39 760.0 40 671.5 41 675.5 42 684.5 43 642.0 44 652.5 45 756.0 46 1374.0 47 3998.0 48 8539.0 49 8770.5 50 5363.5 51 3176.0 52 1508.5 53 663.5 54 407.5 55 318.5 56 289.5 57 276.5 58 237.5 59 192.5 60 191.0 61 181.5 62 160.0 63 139.0 64 115.5 65 107.5 66 105.5 67 111.0 68 95.5 69 78.0 70 66.5 71 52.0 72 36.0 73 26.0 74 25.0 75 22.0 76 11.5 77 6.5 78 6.0 79 3.0 80 3.0 81 3.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3745369735217451 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0034298257648511456 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0034298257648511456 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008917546988612978 36-37 0.02263685004801756 38-39 0.0164631636712855 40-41 0.007545616682672521 42-43 0.006173686376732062 44-45 0.004115790917821375 46-47 0.004801756070791603 48-49 0.0020578954589106874 50-51 6.859651529702291E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0013719303059404582 60-61 0.0020578954589106874 62-63 6.859651529702291E-4 64-65 0.0034298257648511456 66-67 0.0034298257648511456 68-69 0.004801756070791603 70-71 6.859651529702291E-4 72-73 0.0 74-75 0.0 76-77 0.06859651529702292 78-79 0.011661407600493895 80-81 6.859651529702291E-4 82-83 0.0 84-85 0.0 86-87 0.10289477294553437 88-89 0.38757031142817944 90-91 0.3848264508162985 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.859651529702291E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 72890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.11181231993415 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37072637049194 29.624091096172318 2 7.908412456531083 5.553573878446975 3 2.774196069237682 2.9222115516531764 4 1.2972297112491697 1.8219234462889287 5 0.7931856366975345 1.392509260529565 6 0.5353026218106514 1.1277267114830567 7 0.36338061188606263 0.8931266291672383 8 0.26179033329426016 0.7353546439840857 9 0.21880983081311295 0.6914528741939909 >10 1.121400382917204 7.217725339552751 >50 0.1367561442581956 3.2103169159006724 >100 0.1367561442581956 10.969954726299903 >500 0.05470245770327824 14.247496227191657 >1k 0.02735122885163912 19.592536699135685 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3213 4.4080120729866925 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2200 3.018246673069008 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2085 2.860474687885856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2030 2.78501852105913 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1924 2.6395939086294415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 1.9673480587186172 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1395 1.9138427767869393 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 925 1.2690355329949239 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 893 1.2251337632048291 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 877 1.203182878309782 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 875 1.200439017697901 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 870 1.1935793661681986 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 803 1.101660035670188 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 715 0.9809301687474277 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 714 0.9795582384414872 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 703 0.9644670050761422 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 648 0.8890108382494168 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 634 0.8698038139662505 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.8615722321306079 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.7970915077514061 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 519 0.7120318287830978 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 470 0.6448072437920154 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 469 0.6434353134860749 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 462 0.6338318013444917 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 454 0.6228563588969681 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 421 0.577582658800933 RNA PCR Primer, Index 33 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.5007545616682673 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 364 0.4993826313623268 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 318 0.4362738372890657 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 296 0.40609137055837563 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 280 0.3841404856633283 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 276 0.37865276443956647 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 271 0.37179311290986417 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 253 0.3470983674029359 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 240 0.32926327342571 RNA PCR Primer, Index 33 (95% over 24bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 222 0.3045685279187817 RNA PCR Primer, Index 33 (96% over 29bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 211 0.2894772945534367 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 189 0.2592948278227466 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 179 0.24557552476334202 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 176 0.24145973384552066 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 167 0.22911236109205654 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 156 0.2140211277267115 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.21127726711483055 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 147 0.20167375497324738 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 143 0.1961860337494855 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 136 0.1865825216079023 RNA PCR Primer, Index 33 (96% over 27bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 129 0.17697900946631911 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 125 0.17149128824255727 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 120 0.164631636712855 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 119 0.16325970640691453 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 119 0.16325970640691453 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 117 0.1605158457950336 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 116 0.15914391548909315 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.1550281245712718 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.1550281245712718 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 106 0.14542461242968857 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 97 0.13307723967622445 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 90 0.12347372753464124 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 90 0.12347372753464124 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 87 0.11935793661681986 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 83 0.11387021539305804 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.11249828508711757 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 77 0.10563863355741528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 75 0.10289477294553437 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0034298257648511456 0.0 22-23 0.0 0.0 0.0 0.014405268212374811 0.0 24-25 0.0 0.0 0.0 0.030868431883660313 0.0 26-27 0.0 0.0 0.0 0.07751406228563588 0.0 28-29 0.0 0.0 0.0 0.19001234737275347 0.0 30-31 0.0 0.0 0.0 0.3916861023460008 0.0 32-33 0.0 0.0 0.0 0.6989984908766635 0.0 34-35 0.0 0.0 0.0 1.1606530388256278 0.0 36-37 0.0 0.0 0.0 1.7855672931815065 0.0 38-39 0.0 0.0 0.0 2.431746467279462 0.0 40-41 0.0 0.0 0.0 3.0401975579640554 0.0 42-43 0.0 0.0 0.0 3.571134586363013 0.0 44-45 0.0 0.0 0.0 4.099327754150089 0.0 46-47 0.0 0.0 0.0 4.673480587186171 0.0 48-49 0.0 0.0 0.0 5.215393058032651 0.0 50-51 0.0 0.0 0.0 5.677733571134587 0.0 52-53 0.0 0.0 0.0 6.0934284538345445 0.0 54-55 0.0 0.0 0.0 6.629853203457264 0.0 56-57 0.0 0.0 0.0 7.279462203320071 0.0 58-59 0.0 0.0 0.0 7.743174646727946 0.0 60-61 0.0 0.0 0.0 8.23432569625463 0.0 62-63 0.0 0.0 0.0 8.737824118534778 0.0 64-65 0.0 0.0 0.0 9.22760323775552 0.0 66-67 0.0 0.0 0.0 9.76197009191933 0.0 68-69 0.0 0.0 0.0 10.437645767595006 0.0 70-71 0.0 0.0 0.0 11.129098641788996 0.0 72-73 0.0 0.0 0.0 11.695705858142407 0.0 74-75 0.0 0.0 0.0 12.374125394429964 0.0 76-77 0.0 0.0 0.0 12.982576485114556 0.0 78-79 0.0 0.0 0.0 13.89010838249417 0.0 80-81 0.0 0.0 0.0 14.726299903964879 0.0 82-83 0.0 0.0 0.0 15.363561531074222 0.0 84-85 0.0 0.0 0.0 16.14350391000137 0.0 86-87 0.0 0.0 0.0 16.870626972149815 0.0 88-89 0.0 0.0 0.0 17.658800932912605 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGATG 35 3.411691E-6 67.767426 6 CTTTACA 30 1.2927597E-4 63.33654 1 GTACCCA 40 7.4726886E-6 59.378006 1 GGATGCA 25 0.0050287307 56.92464 8 GATGCAA 25 0.0050287307 56.92464 9 GGGTGGT 35 2.7887642E-4 54.213943 8 ACCCATG 45 1.513676E-5 52.708 3 TACCCAT 45 1.513676E-5 52.708 2 GGTGGTA 40 5.389854E-4 47.4372 9 ATGGGAT 85 7.1449904E-9 44.646774 5 GTAAGCG 50 6.366463E-10 42.69348 94-95 GGGGTGG 45 9.6282177E-4 42.1664 7 TGGGGTG 65 0.005846355 29.192122 6 TGGGGGA 35 0.008422136 27.106972 12-13 TGGGTAC 35 0.008422136 27.106972 48-49 GCAGAGA 105 1.2623786E-9 24.848057 94-95 AGTACTC 175 1.4653415E-7 24.396275 7 GAGTACT 1515 0.0 22.231295 12-13 GAGTCTG 65 2.950757E-4 21.89409 92-93 ATGGGGG 55 0.002887535 21.562365 12-13 >>END_MODULE