FastQCFastQC Report
Fri 27 May 2016
SRR939225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.5291475832629285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.4325191461124584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20470.3500856829150662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.2671391483699723No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11120.19017844621473062No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.135108788228091No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7320.12518940883919316No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.11698026727596739No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6180.10569269762653194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7400.039.7066842
GTACCTG8250.035.6676141
ACCTGGG10000.027.961193
ACCGTGC700.00849621327.0717858
GAGTACT26850.022.93791812-13
GTACATG32500.022.4891821
GTATCAA58850.021.7747751
TAAGGGT1754.1243075E-621.6648454
TACATGG32850.021.6401142
GGTATCA46100.021.4140451
GTAGTAC1150.003770582820.605153
GTACTTT29850.020.55412314-15
GTATAGA1856.5897257E-620.5236471
GTAAGGT1656.4276246E-520.1056333
ACATGGG33700.019.8286363
ACGATTA600.004738966519.80936650-51
AGTACTT28850.019.4593112-13
GTCTAAG2001.2920938E-518.9843771
GTACTAG1250.00605636618.9843751
TGTAAGG2502.2200766E-718.956742
Produced by FastQC (version 0.11.5)