##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584714 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10640928727549 33.0 31.0 34.0 30.0 34.0 2 32.18932503753972 34.0 31.0 34.0 30.0 34.0 3 32.243729413012176 34.0 31.0 34.0 30.0 34.0 4 35.684387580937006 37.0 35.0 37.0 35.0 37.0 5 35.74439811600201 37.0 35.0 37.0 35.0 37.0 6 35.82685723276679 37.0 37.0 37.0 35.0 37.0 7 35.76622417113324 37.0 36.0 37.0 35.0 37.0 8 35.76031187896989 37.0 36.0 37.0 35.0 37.0 9 37.44026139274928 39.0 38.0 39.0 35.0 39.0 10-11 37.34565531182766 39.0 38.0 39.0 35.0 39.0 12-13 37.26532544115585 39.0 37.0 39.0 34.0 39.0 14-15 38.555498072561974 40.0 38.0 41.0 34.0 41.0 16-17 38.50732580372627 40.0 38.0 41.0 34.0 41.0 18-19 38.46535400212753 40.0 38.0 41.0 34.0 41.0 20-21 38.38033465933773 40.0 38.0 41.0 34.0 41.0 22-23 38.27810177283253 40.0 38.0 41.0 34.0 41.0 24-25 38.31330359799834 40.0 38.0 41.0 34.0 41.0 26-27 38.22659539535568 40.0 38.0 41.0 34.0 41.0 28-29 38.09847805935894 40.0 38.0 41.0 33.5 41.0 30-31 37.9089854185123 40.0 38.0 41.0 33.0 41.0 32-33 37.67710111268073 40.0 37.5 41.0 33.0 41.0 34-35 37.33650297410358 40.0 37.0 41.0 32.0 41.0 36-37 37.08086517511125 40.0 37.0 41.0 31.0 41.0 38-39 36.844091299336085 40.0 36.5 41.0 30.5 41.0 40-41 36.696422524516265 40.0 36.0 41.0 30.0 41.0 42-43 36.486454061301764 40.0 36.0 41.0 30.0 41.0 44-45 36.179231213892606 40.0 35.5 41.0 29.5 41.0 46-47 35.76637381694299 39.0 35.0 41.0 27.5 41.0 48-49 35.66973597348448 39.5 35.0 41.0 27.0 41.0 50-51 34.65501937699457 38.5 34.0 40.0 25.0 40.5 52-53 34.81326426252835 38.5 35.0 40.0 25.0 40.5 54-55 35.31644616000301 39.0 35.0 41.0 26.0 41.0 56-57 35.20826763169686 39.0 35.0 41.0 26.0 41.0 58-59 35.02993942337621 39.0 35.0 41.0 25.0 41.0 60-61 34.72959857297756 39.0 35.0 41.0 24.5 41.0 62-63 34.37863895853357 38.0 34.5 40.0 23.0 41.0 64-65 34.02244430610521 37.5 34.0 40.0 22.0 41.0 66-67 33.63305393748055 37.0 34.0 40.0 21.5 41.0 68-69 33.241416316352954 36.5 34.0 39.0 20.0 41.0 70-71 32.83652691743313 36.0 33.0 39.0 18.5 41.0 72-73 32.385438864128446 35.0 33.0 38.5 16.5 40.0 74-75 31.93907619793609 35.0 32.0 37.5 12.0 40.0 76-77 31.518536412673548 35.0 32.0 37.0 8.0 39.0 78-79 31.09452398950598 35.0 31.5 36.5 7.0 39.0 80-81 30.672393683065565 35.0 31.0 36.0 2.0 38.5 82-83 30.250617395855066 35.0 31.0 36.0 2.0 37.0 84-85 29.876405387933247 34.5 31.0 35.0 2.0 37.0 86-87 29.5113918941568 34.0 30.0 35.0 2.0 36.5 88-89 29.119002623504826 34.0 30.0 35.0 2.0 36.0 90-91 28.755856709434013 34.0 29.0 35.0 2.0 36.0 92-93 28.36303303837432 34.0 29.0 35.0 2.0 35.0 94-95 27.935106735942703 34.0 29.0 35.0 2.0 35.0 96-97 27.410627246824944 34.0 27.0 35.0 2.0 35.0 98-99 26.76583594714681 34.0 25.5 35.0 2.0 35.0 100-101 24.289489391394767 31.5 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08129921012925223 1101 2 0.23810858267510326 1101 3 0.16238188667366416 1101 4 0.014493366107267036 1101 5 0.1666458777197093 1101 6 0.08861499608413226 1101 7 -0.1929981845484292 1101 8 0.07962779073529447 1101 9 0.09334185439201548 1101 10-11 0.1187759091413838 1101 12-13 -0.09421758046894979 1101 14-15 0.10903614531628847 1101 16-17 0.2523026169841742 1101 18-19 0.12474263818784692 1101 20-21 0.05200579121805049 1101 22-23 0.0987832678792131 1101 24-25 0.0806798033900904 1101 26-27 0.04746685756360591 1101 28-29 -0.17939461597479323 1101 30-31 -0.2790864430606703 1101 32-33 0.11530080527131048 1101 34-35 0.1495156042839909 1101 36-37 0.04268108465796416 1101 38-39 -0.022832174910327296 1101 40-41 0.04675379090348741 1101 42-43 -0.17736606840190916 1101 44-45 -0.32529458109404175 1101 46-47 -0.28734711093654397 1101 48-49 -0.05170788403174953 1101 50-51 0.035376001925143896 1101 52-53 0.20033944645121693 1101 54-55 -0.016977681170544656 1101 56-57 -0.05058801652344158 1101 58-59 -0.01782947663719625 1101 60-61 0.20018550627521137 1101 62-63 0.38550898016285373 1101 64-65 0.10999294501739598 1101 66-67 0.27358076243118035 1101 68-69 0.17026177082244232 1101 70-71 0.22788247097058445 1101 72-73 0.22180986410510428 1101 74-75 0.37995368326767576 1101 76-77 0.03722884300120555 1101 78-79 -0.00959317780750979 1101 80-81 0.3075729805428189 1101 82-83 0.20136700223375925 1101 84-85 -0.0011988573837840022 1101 86-87 0.3878941785142693 1101 88-89 0.26144950501127795 1101 90-91 0.15599118012672974 1101 92-93 -0.044273938645996935 1101 94-95 -0.43015090513711485 1101 96-97 -0.15931286184282456 1101 98-99 -0.31848990630538765 1101 100-101 -0.7615959674574064 1102 1 0.3057471726465195 1102 2 0.3991992055875144 1102 3 0.4025284865986265 1102 4 0.4042551641068428 1102 5 0.39505167216867676 1102 6 0.40456437243962995 1102 7 0.4533958416418926 1102 8 0.14639847441072362 1102 9 0.3902616087776565 1102 10-11 0.3660832107693608 1102 12-13 0.4129248191210877 1102 14-15 0.6171554201865703 1102 16-17 0.5582498885966771 1102 18-19 0.5277368638712048 1102 20-21 0.5126356584362242 1102 22-23 0.5192514359292133 1102 24-25 0.4082807624208087 1102 26-27 0.11368619008554504 1102 28-29 0.4838831094386009 1102 30-31 0.5939060439219475 1102 32-33 0.7228371578432231 1102 34-35 0.7110473081191984 1102 36-37 0.6513087007162426 1102 38-39 0.6068794752996851 1102 40-41 0.6686161224096594 1102 42-43 0.6487615705340062 1102 44-45 0.9141565385377177 1102 46-47 0.8392783454724011 1102 48-49 0.8106716651872148 1102 50-51 0.9069798160854887 1102 52-53 1.1071016196441192 1102 54-55 1.0437395786371013 1102 56-57 1.2423716181663806 1102 58-59 1.3796192088135442 1102 60-61 1.113026268741237 1102 62-63 0.9592596321756304 1102 64-65 1.0448671637459483 1102 66-67 1.3463499636354612 1102 68-69 1.2142394605467288 1102 70-71 1.0684230238774148 1102 72-73 1.1344195623947684 1102 74-75 0.9725166196586805 1102 76-77 0.9162175597485529 1102 78-79 0.843603286224262 1102 80-81 1.0356699268352685 1102 82-83 0.8891787100954325 1102 84-85 0.8161529183787302 1102 86-87 0.46513367081365686 1102 88-89 0.5040096960853404 1102 90-91 0.34605862341306093 1102 92-93 -0.009218528998953701 1102 94-95 -0.20436460060825468 1102 96-97 -0.16548524944497345 1102 98-99 -0.36216561116551205 1102 100-101 -0.2120452843310403 1103 1 0.17208068366286966 1103 2 0.29485929798268273 1103 3 0.21988367494262206 1103 4 0.28892112004145787 1103 5 0.2949184099085471 1103 6 0.3257841663273382 1103 7 0.3411845765388364 1103 8 0.18269467199423417 1103 9 0.3888801233476613 1103 10-11 0.24052573031448787 1103 12-13 0.22654422210616332 1103 14-15 0.37209945003731804 1103 16-17 0.24560107907286977 1103 18-19 0.42304825335236274 1103 20-21 0.48360003299201537 1103 22-23 0.3653068730294322 1103 24-25 0.4497963985259972 1103 26-27 0.3101725084935083 1103 28-29 0.41198145698657385 1103 30-31 0.29248902188925285 1103 32-33 0.3309839240123722 1103 34-35 0.43043030385480563 1103 36-37 0.31334006176812323 1103 38-39 0.08667247458895844 1103 40-41 0.11143994970176863 1103 42-43 -0.22816184808716855 1103 44-45 -0.30256121199533226 1103 46-47 -0.4394840923385459 1103 48-49 -0.2653300228014146 1103 50-51 -0.0830610510076113 1103 52-53 -0.28000747772617984 1103 54-55 -0.24984606457540792 1103 56-57 -0.40852346001414475 1103 58-59 -0.2937683178389108 1103 60-61 -0.04550029486927798 1103 62-63 -0.3618890885066719 1103 64-65 -0.8515458604189377 1103 66-67 -0.6439174492998632 1103 68-69 -0.411851068948657 1103 70-71 -0.38844442459451045 1103 72-73 -0.4433323032768719 1103 74-75 -0.7087489276050043 1103 76-77 -0.6787851054966509 1103 78-79 -0.7296709675070794 1103 80-81 -0.7363960824042515 1103 82-83 -0.7540693353914207 1103 84-85 -0.6947879131777732 1103 86-87 -0.688040192015059 1103 88-89 -0.909973957077419 1103 90-91 -0.8072151861107493 1103 92-93 -0.7889448971581565 1103 94-95 -0.9822252971256695 1103 96-97 -0.7909294569787306 1103 98-99 -0.8902084327717681 1103 100-101 -1.0985058387020423 1104 1 0.2227009779818303 1104 2 0.3179043014049938 1104 3 0.3274769329850571 1104 4 0.22103953208799965 1104 5 0.29982087397425516 1104 6 0.31502526831227584 1104 7 0.22201534313705906 1104 8 0.27718046939327223 1104 9 0.314671533340821 1104 10-11 0.45021301641989453 1104 12-13 0.2639928874039015 1104 14-15 0.32496135968824547 1104 16-17 0.23982806401468793 1104 18-19 0.2798351287801566 1104 20-21 0.303028078851014 1104 22-23 0.3583253535222397 1104 24-25 0.542720765398002 1104 26-27 0.4620419472340984 1104 28-29 0.3905043009290807 1104 30-31 0.38114833058192943 1104 32-33 0.2580972025886936 1104 34-35 0.19265437983016653 1104 36-37 0.19078059907133849 1104 38-39 0.28074759436992736 1104 40-41 0.2317107402561831 1104 42-43 0.2275149144042814 1104 44-45 0.10718596459604868 1104 46-47 0.3106228549578276 1104 48-49 0.5441690360623781 1104 50-51 0.40904196062825093 1104 52-53 0.3110175051622548 1104 54-55 0.3594797396682594 1104 56-57 0.49722816558476524 1104 58-59 0.5916654603951699 1104 60-61 0.38572189883365837 1104 62-63 0.40013606549742775 1104 64-65 0.5559725345160444 1104 66-67 0.04955876562141981 1104 68-69 0.10635777773170219 1104 70-71 0.08618767670596839 1104 72-73 0.10394054066563285 1104 74-75 0.3294077287947239 1104 76-77 0.3713560649345595 1104 78-79 0.1896757984066788 1104 80-81 0.2477008546251831 1104 82-83 0.4664303223772315 1104 84-85 0.4913388835368053 1104 86-87 0.3962663617152664 1104 88-89 0.4058542427856864 1104 90-91 0.3880064086873318 1104 92-93 0.18177721098694732 1104 94-95 -0.18424446208117828 1104 96-97 -0.12084047682814969 1104 98-99 -0.18684173188196596 1104 100-101 -0.19347503788068465 1105 1 0.06123215519198055 1105 2 0.043183834131113485 1105 3 0.11412951627339396 1105 4 0.17886033949123714 1105 5 0.013560444457489496 1105 6 0.23507319383853087 1105 7 0.026281345572748194 1105 8 -0.007160574326945834 1105 9 0.0933706863674999 1105 10-11 0.09145143408927936 1105 12-13 0.3646680224900365 1105 14-15 0.15857977633289977 1105 16-17 0.2042603081195722 1105 18-19 0.14952578054366228 1105 20-21 0.12810779050960974 1105 22-23 0.24208230489123395 1105 24-25 0.1884640248855618 1105 26-27 0.22253255327917998 1105 28-29 0.10547353952016891 1105 30-31 0.054145291882853996 1105 32-33 0.10564003405715994 1105 34-35 -0.05604402436016187 1105 36-37 0.07886109503748173 1105 38-39 0.15017615341172785 1105 40-41 -0.11835251670897406 1105 42-43 -0.13164473356381734 1105 44-45 0.05003595691061946 1105 46-47 -0.17518866687469625 1105 48-49 -0.1658598532556752 1105 50-51 -0.11560263769987245 1105 52-53 0.07425515823032214 1105 54-55 0.10823135065172096 1105 56-57 -0.16869820409603875 1105 58-59 0.02003926373497933 1105 60-61 -0.10069263332098188 1105 62-63 -0.07073025933200938 1105 64-65 -0.18266190328522214 1105 66-67 -0.05106316267158917 1105 68-69 -0.058526132928186314 1105 70-71 -0.011565026002053003 1105 72-73 -0.075524001933303 1105 74-75 -0.22071109970993064 1105 76-77 -0.23083644906670742 1105 78-79 -0.40005844351475517 1105 80-81 -0.41970742277532835 1105 82-83 -0.5029816834400442 1105 84-85 -0.318480331603876 1105 86-87 -0.2606069706458989 1105 88-89 -0.37716845483813444 1105 90-91 -0.31311329871471827 1105 92-93 -0.29842986196941723 1105 94-95 -0.398380127579923 1105 96-97 -0.27045600848224893 1105 98-99 -0.13270283456319376 1105 100-101 -0.30435996538150434 1106 1 0.26470829743900737 1106 2 0.29586637055341214 1106 3 0.23482063681223764 1106 4 0.3174881557118141 1106 5 0.35686490437349505 1106 6 0.4088484344700163 1106 7 0.27260370323010363 1106 8 0.35267468429435667 1106 9 0.5117967900143299 1106 10-11 0.44843240313122124 1106 12-13 0.47409957265966796 1106 14-15 0.4539952064948807 1106 16-17 0.4928835206472897 1106 18-19 0.5296172877927106 1106 20-21 0.588716115402832 1106 22-23 0.37748399480064876 1106 24-25 0.47029147847679553 1106 26-27 0.5587349316177352 1106 28-29 0.33805387396075304 1106 30-31 0.37709080540709294 1106 32-33 0.27767826595579237 1106 34-35 0.1807185879876485 1106 36-37 0.16993984651597316 1106 38-39 0.4236266566307805 1106 40-41 0.400173805790331 1106 42-43 0.33185506458195846 1106 44-45 0.23497338210061258 1106 46-47 0.13543134431582615 1106 48-49 0.37581773242613536 1106 50-51 0.2859580141830378 1106 52-53 0.3686241335899396 1106 54-55 0.2580567644528813 1106 56-57 0.43327005167097354 1106 58-59 0.5735979183234505 1106 60-61 0.587821507098738 1106 62-63 0.32263581924240015 1106 64-65 0.2137178234679027 1106 66-67 0.2334733871084964 1106 68-69 0.23701501346216247 1106 70-71 0.4432745175949151 1106 72-73 0.37067830557921866 1106 74-75 0.2709827759620289 1106 76-77 0.219070060555012 1106 78-79 0.1372916708693026 1106 80-81 0.12386298893646241 1106 82-83 0.07981750347696703 1106 84-85 0.2193956661580181 1106 86-87 0.028499629633159884 1106 88-89 0.03009369359908831 1106 90-91 0.451356462105732 1106 92-93 0.3951571938627545 1106 94-95 0.5464339894671966 1106 96-97 0.873584688162719 1106 98-99 0.6582771145837079 1106 100-101 0.4513096594529422 1107 1 0.1328248945099162 1107 2 0.13370375258353562 1107 3 0.18427657561294097 1107 4 0.12153842656186953 1107 5 0.13794857444236897 1107 6 0.3315161894839491 1107 7 0.23897174899465767 1107 8 0.2195129230606554 1107 9 0.41490465107465724 1107 10-11 0.36219887169169596 1107 12-13 0.2109820648849592 1107 14-15 0.4139984445528597 1107 16-17 0.6174037146609308 1107 18-19 0.48967112050653583 1107 20-21 0.36088332062151096 1107 22-23 0.4285609863749542 1107 24-25 0.2925828092511651 1107 26-27 0.21937802342343105 1107 28-29 0.27871477508529807 1107 30-31 0.16369941189535098 1107 32-33 0.34881603857666477 1107 34-35 0.42223217771221044 1107 36-37 0.22010613428488313 1107 38-39 0.2368362466791467 1107 40-41 0.1475631215482096 1107 42-43 0.1411975364344613 1107 44-45 0.30572832182550513 1107 46-47 0.4538926596419586 1107 48-49 0.3876197791486149 1107 50-51 0.4313889794616088 1107 52-53 0.4376875253207473 1107 54-55 0.38770725657023775 1107 56-57 0.28898758508030653 1107 58-59 0.4079341501684013 1107 60-61 0.46673500372913423 1107 62-63 0.4548042387328053 1107 64-65 0.2714412663330421 1107 66-67 0.07792517714742786 1107 68-69 0.014666679375530123 1107 70-71 0.20185874420500483 1107 72-73 0.1923338301783346 1107 74-75 0.23849932041449406 1107 76-77 0.0539274173552684 1107 78-79 0.10471545845269858 1107 80-81 -0.1194989922153411 1107 82-83 -0.1299681775608299 1107 84-85 -0.08232219105711636 1107 86-87 -0.13297658750561325 1107 88-89 0.1838255554149626 1107 90-91 0.06151957927620799 1107 92-93 -0.08009358698143743 1107 94-95 -0.44362078768021007 1107 96-97 -0.32322683662528817 1107 98-99 0.32581457149392534 1107 100-101 0.5308360260145939 1108 1 0.04362053873533256 1108 2 0.15179770377978485 1108 3 0.10356121117450812 1108 4 0.19189213453421416 1108 5 0.27698362729985604 1108 6 0.33130952864617313 1108 7 0.2819635620460801 1108 8 0.32216930967538815 1108 9 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3882.0 14 3300.0 15 3486.0 16 4331.0 17 6194.0 18 5707.0 19 3419.0 20 3279.0 21 3315.0 22 3498.0 23 3812.0 24 4259.0 25 4701.0 26 5409.0 27 6313.0 28 7348.0 29 8623.0 30 10304.0 31 12784.0 32 16236.0 33 21339.0 34 29551.0 35 44904.0 36 72125.0 37 121768.0 38 133163.0 39 28599.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79090519090519 19.07936507936508 14.332904332904334 21.796825396825398 2 15.10495593374737 20.91276303194996 41.201903401842785 22.780377632459885 3 19.639212678236333 26.48581642856164 29.66119687153287 24.213774021669153 4 12.516502201149631 16.960238898791637 37.770563326501296 32.75269557355744 5 13.13059695741303 37.98746726812819 33.86218105156436 15.019754722894413 6 29.947400042107354 36.316736303556354 18.67906151952771 15.056802134808574 7 27.716360474113433 33.236899226929424 21.484548859566836 17.56219143939031 8 25.959461078869428 34.50362076934929 20.310889699210794 19.22602845257049 9 26.747653249162973 17.62613571785585 21.281829198989406 34.34438183399177 10-11 24.79626577110902 26.988516253378453 27.241590025624042 20.97362794988848 12-13 26.273522693134932 24.604563704164608 27.935121145025686 21.186792457674773 14-15 22.97935166363539 25.74156413822617 27.276944474162836 24.002139723975606 16-17 21.469203600865043 28.936745237685678 27.70268130769698 21.8913698537523 18-19 21.253431182483702 27.533083574917683 30.462652215257858 20.750833027340757 20-21 22.528210018472834 26.361707992282156 30.146156229830112 20.963925759414895 22-23 21.936413413828173 26.38831340937653 30.107233235283037 21.568039941512257 24-25 21.892244320638515 26.55842638042325 30.321064902218147 21.228264396720085 26-27 21.6224755042142 26.40861470808832 30.665795629287405 21.30311415841008 28-29 21.184127614690208 27.01632511883399 30.6227825646224 21.176764701853397 30-31 21.53610009870502 26.500628784370196 30.365705098846274 21.597566018078513 32-33 21.392814648051292 26.825913149176944 30.305852543635137 21.475419659136627 34-35 22.063920766527563 26.743098643751374 29.988554936305732 21.20442565341533 36-37 21.965039760355136 26.49515949685578 30.202144498428318 21.337656244360765 38-39 21.57533658174521 26.948188059680977 30.25850989986736 21.217965458706452 40-41 21.697709692879315 26.95813271172844 30.0435970859694 21.300560509422844 42-43 21.501533184926416 26.983195537138815 29.61774765726422 21.897523620670544 44-45 21.743800190062775 26.894398303526472 29.516869360196456 21.844932146214298 46-47 22.074907796006613 27.288171422089775 29.0012064716376 21.635714310266007 48-49 21.991527936410538 27.224647190717683 29.048487051699528 21.73533782117225 50-51 21.664730387439164 27.622741771386146 29.030783402535263 21.681744438639424 52-53 21.995148204895965 27.885963050392153 28.471340808959063 21.647547935752815 54-55 21.644583484053452 27.55089327984452 28.68462362644208 22.11989960965995 56-57 21.93942371870917 27.629134134912327 28.52277129743609 21.908670848942418 58-59 22.136212008258813 27.293332142378635 28.64543789915591 21.925017950206644 60-61 21.94884500773944 27.440799510307663 29.140986578961602 21.469368902991295 62-63 21.698759170053755 27.59121942921651 29.04313565794572 21.66688574278402 64-65 21.552101971258708 27.68218545197667 28.864528171519616 21.901184405245004 66-67 21.626511253342226 27.70289491981666 28.386509568800218 22.284084258040895 68-69 21.492290474364903 27.517995196696482 28.7716849211684 22.218029407770214 70-71 21.802505977869423 27.593088878154603 28.512932998011102 22.091472145964875 72-73 21.795763178724624 27.294056779146086 28.77116025717603 22.13901978495326 74-75 21.47395979718957 28.129504379642977 28.257313801784516 22.13922202138294 76-77 21.752337512685425 27.452594529519985 28.395591100050954 22.39947685774363 78-79 21.727711808055314 27.524437243234978 28.523318688455863 22.22453226025385 80-81 21.832272763589597 27.75849078648828 28.219230805577677 22.190005644344442 82-83 21.946380577265447 27.57588724145753 28.302796279875448 22.174935901401575 84-85 21.673418711254506 27.681521164759914 28.340199940713756 22.304860183271828 86-87 21.65603344202852 27.676715600981268 28.366865583151817 22.3003853738384 88-89 21.819463064177924 27.51495307827163 28.10637310508405 22.5592107524664 90-91 21.77686706932281 27.360562331910256 28.315488280024514 22.54708231874242 92-93 21.95419823709795 27.447453280345428 28.33672529255648 22.261623190000137 94-95 21.712448860315607 27.286846357479288 28.442775122907136 22.55792965929797 96-97 21.721623212819996 27.107465008444386 28.636478884739514 22.534432893996108 98-99 21.85589373284357 27.24127202941323 28.135511938382102 22.767322299361098 100-101 21.95699155728372 26.585490558336378 28.575221809276247 22.882296075103653 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 884.0 1 543.5 2 429.5 3 553.0 4 355.0 5 276.0 6 384.5 7 705.0 8 1115.5 9 1340.0 10 1256.0 11 1040.5 12 1038.5 13 1155.0 14 1200.5 15 1255.5 16 1317.0 17 1355.0 18 1374.0 19 1434.5 20 1563.0 21 1719.0 22 1971.0 23 2284.0 24 2615.5 25 3125.5 26 3928.0 27 4676.5 28 5311.5 29 6124.5 30 7094.0 31 8301.5 32 9706.5 33 10855.0 34 12074.5 35 13259.5 36 14534.5 37 16090.5 38 16968.5 39 17360.0 40 17418.0 41 17752.5 42 18325.5 43 18928.0 44 19717.0 45 20361.0 46 20522.5 47 21750.0 48 25464.5 49 25132.0 50 21569.5 51 19781.5 52 18517.5 53 17916.0 54 17251.5 55 16020.0 56 14423.0 57 12870.5 58 11706.5 59 10329.0 60 8640.5 61 7213.0 62 5915.5 63 4580.0 64 3446.5 65 2626.5 66 1963.0 67 1550.0 68 1215.5 69 913.5 70 694.0 71 498.5 72 403.5 73 329.0 74 235.5 75 185.0 76 130.0 77 81.5 78 59.0 79 42.0 80 26.0 81 19.0 82 12.5 83 10.0 84 8.0 85 4.5 86 5.0 87 4.0 88 2.5 89 2.0 90 1.5 91 2.0 92 1.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33589070896198825 2 0.12091381427501308 3 0.11253364892922009 4 0.11988767157960986 5 0.13733209740146465 6 0.08431472480563147 7 0.09406308041196208 8 0.09987788901924702 9 0.0858539388487363 10-11 0.08499881993590028 12-13 0.08850480747852797 14-15 0.0900440215216328 16-17 0.07961157078503336 18-19 0.09397756852067848 20-21 0.10483757871369592 22-23 0.1128756964943545 24-25 0.1257879920781784 26-27 0.125873503969462 28-29 0.12074279049244588 30-31 0.11125097055996608 32-33 0.10415348358342712 34-35 0.3300759003547033 36-37 0.4879308516642324 38-39 0.4593698799755094 40-41 0.43867600228487774 42-43 0.4712560328639301 44-45 0.4790376149707378 46-47 0.4877598278816652 48-49 0.4993039332049515 50-51 0.48604959005599313 52-53 0.4904106965114569 54-55 0.47638674634094613 56-57 0.4539826308246424 58-59 0.4357685979812353 60-61 0.3946373782738228 62-63 0.46595429560434676 64-65 0.49648204079259267 66-67 0.5051187418122365 68-69 0.5183730849611947 70-71 0.5105059909631033 72-73 0.5016982661608924 74-75 0.5111045742020885 76-77 0.4866481732949784 78-79 0.46398752210482386 80-81 0.46441508156124195 82-83 0.4785245436230362 84-85 0.4774128890363494 86-87 0.48083336468769344 88-89 0.49494282674948775 90-91 0.5119596931149246 92-93 0.5038360634429824 94-95 0.508624729354864 96-97 0.50734205098561 98-99 0.5019548018347432 100-101 0.48767431599038163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 584714.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.29349861968268 #Duplication Level Percentage of deduplicated Percentage of total 1 86.91431606754186 61.96425672591961 2 8.212712143075262 11.710259636723746 3 2.0711875495303236 4.4298662011063215 4 0.8806932095912613 2.51150800489434 5 0.4649396179977803 1.6573586006980268 6 0.3066602547183763 1.3117729467885662 7 0.20814596115882222 1.0387617656198311 8 0.15883906445527293 0.9059354097994953 9 0.1144025201332533 0.7340540320087482 >10 0.6162061567911252 7.738199832515271 >50 0.03579965361610243 1.7038621181960836 >100 0.01345487877419337 1.8391817248497537 >500 0.0014415941543683307 0.6831197465469161 >1k 0.0012013284619736089 1.7718632543333248 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3094 0.5291475832629285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.4325191461124584 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2047 0.3500856829150662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.2671391483699723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1112 0.19017844621473062 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 790 0.135108788228091 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 732 0.12518940883919316 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.11698026727596739 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 618 0.10569269762653194 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.710237825672038E-4 0.0 0.0 0.0 0.0 3 1.710237825672038E-4 0.0 0.0 0.0 0.0 4 1.710237825672038E-4 0.0 0.0 0.0 0.0 5 1.710237825672038E-4 0.0 0.0 0.0 0.0 6 1.710237825672038E-4 0.0 0.0 0.0 0.0 7 1.710237825672038E-4 0.0 0.0 0.0 0.0 8 1.710237825672038E-4 0.0 0.0 0.0 0.0 9 1.710237825672038E-4 0.0 0.0 0.0 0.0 10-11 1.710237825672038E-4 0.0 0.0 0.0 0.0 12-13 1.710237825672038E-4 0.0 0.0 0.0 0.0 14-15 1.710237825672038E-4 1.710237825672038E-4 0.0 0.0 0.0 16-17 1.710237825672038E-4 1.710237825672038E-4 0.0 0.0 0.0 18-19 1.710237825672038E-4 1.710237825672038E-4 0.0 0.0 0.0 20-21 1.710237825672038E-4 1.710237825672038E-4 0.0 0.0 0.0 22-23 1.710237825672038E-4 1.710237825672038E-4 0.0 8.55118912836019E-4 0.0 24-25 1.710237825672038E-4 1.710237825672038E-4 0.0 0.001453702151821232 0.0 26-27 2.565356738508057E-4 1.710237825672038E-4 0.0 0.0021377972820900474 0.0 28-29 5.130713477016114E-4 1.710237825672038E-4 0.0 0.004190082672896493 0.0 30-31 5.130713477016114E-4 1.710237825672038E-4 0.0 0.010603474519166635 0.0 32-33 5.130713477016114E-4 1.710237825672038E-4 0.0 0.022062067951169287 0.0 34-35 5.130713477016114E-4 1.710237825672038E-4 0.0 0.03967751755559128 0.0 36-37 6.840951302688152E-4 1.710237825672038E-4 0.0 0.06610069196222426 0.0 38-39 6.840951302688152E-4 1.710237825672038E-4 0.0 0.10825805436504 0.0 40-41 6.840951302688152E-4 1.710237825672038E-4 0.0 0.14725147679036246 0.0 42-43 7.69607021552417E-4 1.710237825672038E-4 0.0 0.19590774293073193 0.0 44-45 9.406308041196208E-4 1.710237825672038E-4 0.0 0.2525166149604764 0.0 46-47 0.0010261426954032227 1.710237825672038E-4 0.0 0.32656991281207565 0.0 48-49 0.0011971664779704266 1.710237825672038E-4 0.0 0.4013928176852273 0.0 50-51 0.0011971664779704266 1.710237825672038E-4 0.0 0.48981211327247165 0.0 52-53 0.0011971664779704266 1.710237825672038E-4 0.0 0.5739558142955359 0.0 54-55 0.0011971664779704266 1.710237825672038E-4 0.0 0.6749453579014698 0.0 56-57 0.0011971664779704266 1.710237825672038E-4 0.0 0.7966287791980353 0.0 58-59 0.0011971664779704266 1.710237825672038E-4 0.0 0.9289156750137674 0.0 60-61 0.0011971664779704266 1.710237825672038E-4 0.0 1.0710364383271136 0.0 62-63 0.0011971664779704266 1.710237825672038E-4 0.0 1.2188864983564613 0.0 64-65 0.0011971664779704266 1.710237825672038E-4 0.0 1.381102556121454 0.0 66-67 0.0013681902605376303 1.710237825672038E-4 0.0 1.5730767520531406 0.0 68-69 0.0013681902605376303 1.710237825672038E-4 0.0 1.788652229979101 0.0 70-71 0.001539214043104834 1.710237825672038E-4 0.0 2.0248360737044093 0.0 72-73 0.001539214043104834 1.710237825672038E-4 0.0 2.280260092968528 0.0 74-75 0.001539214043104834 1.710237825672038E-4 0.0 2.540387266253245 0.0 76-77 0.001539214043104834 1.710237825672038E-4 0.0 2.8203531983157575 0.0 78-79 0.001539214043104834 1.710237825672038E-4 0.0 3.1478637419319533 0.0 80-81 0.001710237825672038 1.710237825672038E-4 0.0 3.492818711370003 0.0 82-83 0.001710237825672038 1.710237825672038E-4 0.0 3.8500018812616084 0.0 84-85 0.001710237825672038 1.710237825672038E-4 0.0 4.213940490564617 0.0 86-87 0.001710237825672038 1.710237825672038E-4 0.0 4.612169368272352 0.0 88-89 0.001710237825672038 1.710237825672038E-4 0.0 5.042978276559138 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 740 0.0 39.706684 2 GTACCTG 825 0.0 35.667614 1 ACCTGGG 1000 0.0 27.96119 3 ACCGTGC 70 0.008496213 27.071785 8 GAGTACT 2685 0.0 22.937918 12-13 GTACATG 3250 0.0 22.489182 1 GTATCAA 5885 0.0 21.774775 1 TAAGGGT 175 4.1243075E-6 21.664845 4 TACATGG 3285 0.0 21.640114 2 GGTATCA 4610 0.0 21.414045 1 GTAGTAC 115 0.0037705828 20.60515 3 GTACTTT 2985 0.0 20.554123 14-15 GTATAGA 185 6.5897257E-6 20.523647 1 GTAAGGT 165 6.4276246E-5 20.105633 3 ACATGGG 3370 0.0 19.828636 3 ACGATTA 60 0.0047389665 19.809366 50-51 AGTACTT 2885 0.0 19.45931 12-13 GTCTAAG 200 1.2920938E-5 18.984377 1 GTACTAG 125 0.006056366 18.984375 1 TGTAAGG 250 2.2200766E-7 18.95674 2 >>END_MODULE