FastQCFastQC Report
Fri 27 May 2016
SRR939225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.48827289922936684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.4043002219888698No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20720.3543612774792463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.2707306478038836No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11140.19052049377986505No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7460.12758374179513404No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.11629617214569858No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6400.10945522084301043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.10295631710545668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6850.051.9426042
GTACCTG8550.041.75051
TAACGCA651.3371429E-436.4930044
ACCTGGG10750.032.6569983
ATAACGC804.5238275E-429.6505683
TATAACG856.450163E-427.9064162
TTCAACG700.00843934327.109092
CGATAAT806.317714E-826.6855148-49
TACATGG34100.023.511772
GTACATG34450.023.486931
AGTACTT28350.022.50916312-13
GTATAAC2354.5311026E-922.2787781
ACATGGG34550.022.2443643
GTACAAC1100.002842337121.6343521
GTTCGTC550.002875317821.59359680-81
CATGGGA20000.021.5856134
GTAGCAC1553.9398343E-521.4249273
GTAGGGT2005.441525E-721.348413
GAGTACT28150.020.89938212-13
GTATCAA57950.020.7794281
Produced by FastQC (version 0.11.5)