##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939225_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584714 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51976521855129 34.0 31.0 34.0 31.0 34.0 2 32.71645625040618 34.0 31.0 34.0 31.0 34.0 3 32.80267960062526 34.0 31.0 34.0 31.0 34.0 4 36.25943794744097 37.0 35.0 37.0 35.0 37.0 5 36.22955154143735 37.0 37.0 37.0 35.0 37.0 6 36.269571106558075 37.0 37.0 37.0 35.0 37.0 7 36.227167469908366 37.0 37.0 37.0 35.0 37.0 8 36.22063607165212 37.0 37.0 37.0 35.0 37.0 9 37.965215472863655 39.0 38.0 39.0 35.0 39.0 10-11 37.906990426088655 39.0 38.0 39.0 35.0 39.0 12-13 37.843385826232996 39.0 38.0 39.0 35.0 39.0 14-15 39.19573586402925 40.5 39.0 41.0 36.0 41.0 16-17 39.14465191529534 40.0 38.5 41.0 36.0 41.0 18-19 39.19406923726814 40.5 38.5 41.0 36.0 41.0 20-21 39.26472856815469 41.0 39.0 41.0 36.0 41.0 22-23 39.213028933803535 41.0 39.0 41.0 36.0 41.0 24-25 39.15725636807054 41.0 39.0 41.0 35.0 41.0 26-27 39.01008099686342 40.5 39.0 41.0 35.0 41.0 28-29 38.862916742202174 40.0 38.0 41.0 35.0 41.0 30-31 38.659159862770515 40.0 38.0 41.0 35.0 41.0 32-33 38.486517340101315 40.0 38.0 41.0 34.0 41.0 34-35 38.40953098437869 40.0 38.0 41.0 34.0 41.0 36-37 38.29331177293514 40.0 38.0 41.0 34.0 41.0 38-39 38.154229247119105 40.0 38.0 41.0 34.0 41.0 40-41 37.99244844488075 40.0 38.0 41.0 33.0 41.0 42-43 37.81893541115828 40.0 38.0 41.0 33.0 41.0 44-45 37.55549037649176 40.0 37.0 41.0 33.0 41.0 46-47 37.21489138279569 40.0 37.0 41.0 32.0 41.0 48-49 36.86756773396908 40.0 37.0 41.0 31.0 41.0 50-51 36.60836665446697 40.0 36.0 41.0 31.0 41.0 52-53 36.384043310062694 40.0 36.0 41.0 30.0 41.0 54-55 36.154442171728405 39.0 35.5 41.0 30.0 41.0 56-57 35.87935127258797 39.0 35.0 41.0 29.0 41.0 58-59 35.595370557229685 39.0 35.0 40.0 28.0 41.0 60-61 35.23800353677183 38.0 35.0 40.0 28.0 41.0 62-63 34.858821577728605 38.0 34.0 40.0 27.0 41.0 64-65 34.460120674380974 37.0 34.0 40.0 26.0 41.0 66-67 34.09549198411531 37.0 34.0 39.5 26.0 41.0 68-69 33.63710207041392 36.0 33.5 39.0 25.5 40.5 70-71 33.20573476947705 36.0 33.0 39.0 24.5 40.0 72-73 32.73777265466536 35.0 33.0 38.0 23.5 40.0 74-75 32.24105716640956 35.0 32.0 37.0 22.0 39.0 76-77 30.26308588472313 33.5 29.5 35.5 19.0 37.5 78-79 31.22638247074638 34.5 31.0 36.0 20.0 39.0 80-81 31.249618616964874 35.0 31.5 36.0 20.0 37.5 82-83 30.949623234607003 35.0 31.0 35.5 18.0 37.0 84-85 30.56285808104475 34.5 31.0 35.0 15.0 37.0 86-87 30.019591629411988 34.0 30.5 35.0 7.0 36.0 88-89 29.468871106216028 34.0 30.0 35.0 2.0 36.0 90-91 29.010476061801157 34.0 29.0 35.0 2.0 35.5 92-93 28.738436226941715 34.0 29.0 35.0 2.0 35.0 94-95 28.42565681683695 34.0 29.0 35.0 2.0 35.0 96-97 27.88638035005148 34.0 29.0 35.0 2.0 35.0 98-99 27.14573107536334 34.0 27.0 35.0 2.0 35.0 100-101 23.58482266543986 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10801839236112443 1101 2 0.12548472515831577 1101 3 0.17483528005066518 1101 4 0.10056301278802238 1101 5 0.11449983635414185 1101 6 0.08767628893354384 1101 7 0.05876275774788553 1101 8 0.08758652269359857 1101 9 0.08839697952305414 1101 10-11 0.16674726560941622 1101 12-13 0.03823428584469468 1101 14-15 0.2633746053973951 1101 16-17 0.1396611523622795 1101 18-19 0.12459104588123893 1101 20-21 0.02697584836282374 1101 22-23 0.1591741380834506 1101 24-25 0.09260376353029898 1101 26-27 0.0578059467091947 1101 28-29 0.07448790546511219 1101 30-31 0.10425105062522988 1101 32-33 0.12821706286195678 1101 34-35 0.10151664888370249 1101 36-37 0.21127920442908987 1101 38-39 0.2044708264654247 1101 40-41 0.24969591827862558 1101 42-43 0.378630988687533 1101 44-45 0.42151095182039455 1101 46-47 -0.17695672287224085 1101 48-49 -0.21424483647579962 1101 50-51 -0.13289040460286827 1101 52-53 0.09916409117627012 1101 54-55 -0.0777515841168892 1101 56-57 -0.06369941030035164 1101 58-59 0.12022242115995141 1101 60-61 0.2843462751345456 1101 62-63 0.1638282776536073 1101 64-65 0.07400120234663632 1101 66-67 -0.05426589572002172 1101 68-69 -0.18059040305018925 1101 70-71 -0.2608585448248917 1101 72-73 -0.06429697718179739 1101 74-75 -0.017589485057058596 1101 76-77 -0.04804634055448176 1101 78-79 -0.026226460467761115 1101 80-81 0.1095864258994439 1101 82-83 0.10829674133711364 1101 84-85 -0.034710283933691244 1101 86-87 -0.22304057427755453 1101 88-89 0.19776173025375243 1101 90-91 0.35661429892660124 1101 92-93 0.032162548989415285 1101 94-95 -0.1558623900214151 1101 96-97 0.021615364931598435 1101 98-99 -0.11611688308621027 1101 100-101 0.4680904458824884 1102 1 0.16767412961598893 1102 2 0.15611788675682448 1102 3 0.232308490332791 1102 4 0.13011634972077246 1102 5 0.08653878325056041 1102 6 0.18123669048190294 1102 7 0.193205504162961 1102 8 0.177102157273616 1102 9 0.20503634553786299 1102 10-11 0.28016774750405204 1102 12-13 0.3148615724649346 1102 14-15 0.3923312934470218 1102 16-17 0.38161508594391336 1102 18-19 0.2959148377130063 1102 20-21 0.15064780128978583 1102 22-23 0.3824416143135849 1102 24-25 0.25374014172516013 1102 26-27 0.2895084092584952 1102 28-29 0.21534325471518656 1102 30-31 0.3219661233303768 1102 32-33 0.39378758547879755 1102 34-35 0.3580825319148886 1102 36-37 0.5714556367982127 1102 38-39 0.42687810338554755 1102 40-41 0.2958515594474065 1102 42-43 0.13513738985154333 1102 44-45 0.01725388034149944 1102 46-47 0.128484952869222 1102 48-49 0.3409268830992289 1102 50-51 0.14411665086169023 1102 52-53 0.041333989691054285 1102 54-55 -0.0029508483719524747 1102 56-57 0.1748297403900949 1102 58-59 0.18780983662706774 1102 60-61 0.15818294707831626 1102 62-63 0.07070026352437253 1102 64-65 -0.07360076330029841 1102 66-67 -0.018629393985897025 1102 68-69 -0.013384586638686358 1102 70-71 -0.06900206999487324 1102 72-73 -0.08280956445999976 1102 74-75 0.01582102218600312 1102 76-77 0.06207518547569535 1102 78-79 -0.16658307713915477 1102 80-81 -0.22794114165822776 1102 82-83 -0.012968483733121872 1102 84-85 0.3176259807880726 1102 86-87 0.0879763826301243 1102 88-89 0.051166380820660606 1102 90-91 0.46450296717334894 1102 92-93 0.4551678474511256 1102 94-95 0.0058184760345731945 1102 96-97 0.15566919144586677 1102 98-99 -0.25909527744184047 1102 100-101 -1.0734782282209387 1103 1 0.1404486498718498 1103 2 -0.016781011608493657 1103 3 0.08273048748986156 1103 4 0.04156801994109571 1103 5 -4.947988389929492E-4 1103 6 -0.04821426900637249 1103 7 0.007895447304399283 1103 8 0.017280728702182557 1103 9 -0.054065466828880915 1103 10-11 0.09919093513731525 1103 12-13 0.15513342747559733 1103 14-15 0.19443683715704907 1103 16-17 0.2130427689574148 1103 18-19 0.08321050224747495 1103 20-21 0.1370661559450781 1103 22-23 0.07373958872722852 1103 24-25 0.1700492213271474 1103 26-27 -0.20583407475003668 1103 28-29 0.02195750489286752 1103 30-31 0.20599908352937746 1103 32-33 0.29053376529400765 1103 34-35 0.10194136276066246 1103 36-37 0.12198646480105424 1103 38-39 0.09185011831091572 1103 40-41 -0.22213884566986764 1103 42-43 -0.21149329600488898 1103 44-45 0.23411377728534433 1103 46-47 0.15917525137668775 1103 48-49 0.08280899041976397 1103 50-51 0.1600459330223174 1103 52-53 0.2792248923207623 1103 54-55 -0.023486562657666354 1103 56-57 0.07948281430622472 1103 58-59 0.30826337251903624 1103 60-61 0.12956265567817837 1103 62-63 -0.025392931216210002 1103 64-65 -0.07923109379070326 1103 66-67 -0.043509457951778074 1103 68-69 0.003002229281712232 1103 70-71 -0.24646297973190912 1103 72-73 -0.4438481216739305 1103 74-75 -0.4454551860584921 1103 76-77 -0.2823811045029849 1103 78-79 -0.42385253342913387 1103 80-81 -0.20513996895034126 1103 82-83 -0.1078334446428606 1103 84-85 -0.25489089909820706 1103 86-87 -0.08301464437769823 1103 88-89 -0.2553945644529705 1103 90-91 0.2857094348350451 1103 92-93 0.48803254183634337 1103 94-95 0.05566300339775765 1103 96-97 -0.04734475667068949 1103 98-99 0.06596913265055449 1103 100-101 -0.42631227228632085 1104 1 0.08922945069321031 1104 2 0.1020117673100529 1104 3 0.17008076127493865 1104 4 0.08829115478025074 1104 5 0.11286505742383923 1104 6 0.03666766117843423 1104 7 0.1026550297821558 1104 8 0.13456768968781319 1104 9 -0.020582407280620885 1104 10-11 0.2106064895521058 1104 12-13 0.09504615848175746 1104 14-15 0.19430850040139802 1104 16-17 0.13008161221545578 1104 18-19 0.20047607377382093 1104 20-21 0.04754057415178892 1104 22-23 0.12116001993872771 1104 24-25 0.15422743830182384 1104 26-27 0.18753733182081334 1104 28-29 0.2632519949681509 1104 30-31 0.31422760851226883 1104 32-33 0.28503025742268306 1104 34-35 0.2592287515354741 1104 36-37 0.33439876801803337 1104 38-39 0.28386115527190725 1104 40-41 0.49513528164019505 1104 42-43 0.2967223953024387 1104 44-45 0.48069788063921237 1104 46-47 0.25787905014465196 1104 48-49 0.2069641067784289 1104 50-51 0.4490688625226511 1104 52-53 0.24011640498332554 1104 54-55 0.4583281190671826 1104 56-57 0.5658513119106061 1104 58-59 0.5522336839495665 1104 60-61 0.5344608418520309 1104 62-63 0.6068687902074714 1104 64-65 0.5656290191456392 1104 66-67 0.5607128213090036 1104 68-69 0.4465896693912299 1104 70-71 0.5037509148608379 1104 72-73 0.3283584149448231 1104 74-75 -0.08496793417621973 1104 76-77 -0.1178020490615026 1104 78-79 0.09535306205341243 1104 80-81 -0.07132323383740413 1104 82-83 0.016687431469392777 1104 84-85 -0.05376581354037313 1104 86-87 0.06662815165242009 1104 88-89 -0.10567262742353734 1104 90-91 0.21225803168651325 1104 92-93 0.427904632869879 1104 94-95 0.523394865136293 1104 96-97 0.2127831522957706 1104 98-99 0.25062219562112276 1104 100-101 -0.029794476256206792 1105 1 0.06759596273173685 1105 2 0.17486566145612414 1105 3 0.13678119126465305 1105 4 0.10653123171332624 1105 5 0.04267938542204064 1105 6 -0.015080871693513132 1105 7 -0.030200362036609363 1105 8 -2.973195040567589E-5 1105 9 0.04726610706747181 1105 10-11 0.06193685644250024 1105 12-13 0.034140145325885385 1105 14-15 -0.027132260731626445 1105 16-17 0.043181124683584926 1105 18-19 0.07839604287447344 1105 20-21 -0.15593008525773655 1105 22-23 -0.007054557146091156 1105 24-25 0.060942135594586944 1105 26-27 0.08932547419430392 1105 28-29 0.05360737693381168 1105 30-31 0.06903691142445467 1105 32-33 0.046253934839747046 1105 34-35 0.19532148112278946 1105 36-37 0.18744751726426045 1105 38-39 0.11169776706331902 1105 40-41 0.07188706603838568 1105 42-43 0.02583675837770727 1105 44-45 -0.05501570128151201 1105 46-47 -0.07723309795152744 1105 48-49 -0.06347419895962503 1105 50-51 -0.0321885519424967 1105 52-53 -0.12395009168435678 1105 54-55 -0.02913675459624443 1105 56-57 0.07024776952055589 1105 58-59 -0.22084131398128193 1105 60-61 0.09984016687459274 1105 62-63 0.06738737908449366 1105 64-65 -0.03757442392505794 1105 66-67 -0.20446195315332005 1105 68-69 -0.2937867982294904 1105 70-71 -0.1511734723742606 1105 72-73 -0.46450951002965013 1105 74-75 -0.23299317390237917 1105 76-77 -0.13564406035710874 1105 78-79 -0.12059557565562429 1105 80-81 -0.25671146607125905 1105 82-83 -0.06894988738118357 1105 84-85 -0.14058747619353795 1105 86-87 -0.09592451321966422 1105 88-89 0.31137207022393554 1105 90-91 0.17106612069556704 1105 92-93 0.08713482910441783 1105 94-95 -0.3256909633328888 1105 96-97 -0.5529729556470215 1105 98-99 -0.3871518686290365 1105 100-101 -0.3276638397847229 1106 1 0.08918019968734825 1106 2 0.11127095641118956 1106 3 0.2114743374283634 1106 4 0.2078195574564745 1106 5 0.042148190090891546 1106 6 0.14602004833680127 1106 7 0.19349661581608046 1106 8 0.13413958479074495 1106 9 0.27750741139990254 1106 10-11 0.3102033890118605 1106 12-13 0.15176626880401045 1106 14-15 0.21265639435262074 1106 16-17 0.15085795960932558 1106 18-19 0.2941268614110655 1106 20-21 0.3271991510250274 1106 22-23 0.24105969942833383 1106 24-25 0.19872003928532678 1106 26-27 0.21112856362635313 1106 28-29 0.3273464962827788 1106 30-31 0.2959706394949322 1106 32-33 0.3674518157245146 1106 34-35 0.18763862598329695 1106 36-37 0.3368196443828495 1106 38-39 0.4523090666504004 1106 40-41 0.44783347905337934 1106 42-43 0.4133260459385326 1106 44-45 0.5323110405079774 1106 46-47 0.556845915583331 1106 48-49 0.45398442092407265 1106 50-51 0.6353380609435959 1106 52-53 0.6801627766996106 1106 54-55 0.6545346550545119 1106 56-57 0.7119051697797829 1106 58-59 0.5716897255325648 1106 60-61 0.6140106876584994 1106 62-63 0.7773246272093743 1106 64-65 0.6520304375746093 1106 66-67 0.6463660033028304 1106 68-69 0.7335449722091454 1106 70-71 0.9762461223591217 1106 72-73 0.7405230345376381 1106 74-75 0.8338152628959961 1106 76-77 0.9176188954970179 1106 78-79 0.9747752340985407 1106 80-81 0.7840436103854529 1106 82-83 0.7901062847544331 1106 84-85 0.7956549188599702 1106 86-87 0.9832253617723623 1106 88-89 0.8000098020906989 1106 90-91 0.960909311833813 1106 92-93 1.1001827570884934 1106 94-95 1.2054246885146078 1106 96-97 1.305265175678631 1106 98-99 1.4150504672139022 1106 100-101 1.0491643574799738 1107 1 0.08502131897606091 1107 2 0.10155003653288475 1107 3 0.11593813526560837 1107 4 0.03883340690026671 1107 5 0.011993775201226242 1107 6 0.10368244031721474 1107 7 0.14184959111914708 1107 8 0.14524005228536652 1107 9 0.21637557521865602 1107 10-11 0.2485453684097081 1107 12-13 0.17704841833482732 1107 14-15 0.26242129785175194 1107 16-17 0.2454429822419968 1107 18-19 0.2534268337526484 1107 20-21 0.2574291206007828 1107 22-23 0.22359105125008227 1107 24-25 0.23057443461173222 1107 26-27 0.2918978722335126 1107 28-29 0.3014555243931696 1107 30-31 0.4340974229565546 1107 32-33 0.41509005718919667 1107 34-35 0.33532611291300896 1107 36-37 0.5091113124549196 1107 38-39 0.38974202873139063 1107 40-41 0.4169150848602996 1107 42-43 0.6339153724899376 1107 44-45 0.6214976133609085 1107 46-47 0.5146482861116013 1107 48-49 0.6571253371595631 1107 50-51 0.38217420846411443 1107 52-53 0.5492507911629332 1107 54-55 0.8211546622052239 1107 56-57 0.6465284419722863 1107 58-59 0.5779179276683379 1107 60-61 0.5976804192369798 1107 62-63 0.5810494057734346 1107 64-65 0.6296068860996087 1107 66-67 0.5065476718471231 1107 68-69 0.491854301189079 1107 70-71 0.6751168496404247 1107 72-73 0.6451639136703804 1107 74-75 0.7915096981584497 1107 76-77 1.0314214549120102 1107 78-79 0.7711486091668469 1107 80-81 0.6834226453092569 1107 82-83 0.9527546114205165 1107 84-85 0.8978932264350021 1107 86-87 0.966918704190249 1107 88-89 1.1651531198858507 1107 90-91 1.2125452057064656 1107 92-93 1.3000164693195835 1107 94-95 1.23237308871159 1107 96-97 1.243779176906397 1107 98-99 1.2393942545274577 1107 100-101 0.4189421457514584 1108 1 -0.04058395882380239 1108 2 0.09475884346397123 1108 3 0.01720512517102435 1108 4 0.056386860520802884 1108 5 0.08760302724796531 1108 6 -0.04397513857158941 1108 7 0.07374689657976319 1108 8 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-0.5708870802492534 2316 96-97 -0.7678526508572254 2316 98-99 -0.7609612834933195 2316 100-101 -0.6971456056196565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 9.0 6 33.0 7 83.0 8 140.0 9 282.0 10 458.0 11 765.0 12 1306.0 13 1920.0 14 2117.0 15 2658.0 16 3864.0 17 5669.0 18 5354.0 19 3394.0 20 2905.0 21 3018.0 22 3069.0 23 3389.0 24 3808.0 25 4403.0 26 5312.0 27 6175.0 28 7567.0 29 9266.0 30 11260.0 31 14122.0 32 17361.0 33 22553.0 34 31511.0 35 47250.0 36 76623.0 37 135771.0 38 135771.0 39 15519.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86431265680719 19.588757589382244 14.761412817775888 21.78551693603468 2 15.295340970115303 21.146919690652183 40.61404378892929 22.943695550303225 3 19.43616879363244 26.454642782625353 29.93531880543308 24.173869618309123 4 12.71031649661202 17.289478274848904 37.476954545299066 32.523250683240015 5 13.095804102518496 37.94846711383685 34.09324900720694 14.862479776437713 6 30.098304470219627 36.37795571852222 18.522730770941006 15.001009040317145 7 27.702432300235667 33.39461685541992 21.296223452833352 17.60672739151106 8 26.021952612730324 34.65335189511454 20.128473065464483 19.196222426690657 9 26.54802176790704 17.51574273918531 21.215842275026763 34.72039321788088 10-11 24.76518434653523 27.08845692081941 27.1537024938688 20.992656238776565 12-13 26.06616226052395 24.622208464309047 27.911850921989213 21.399778353177794 14-15 22.836402381355512 25.825068538366946 27.103567757636704 24.234961322640835 16-17 21.271641403041897 29.02449286045346 27.684149676501324 22.01971606000332 18-19 21.124088015679458 27.668655103178647 30.191426919827467 21.01582996131442 20-21 22.37025280735539 26.434633000064988 30.10326415991408 21.091850032665544 22-23 21.929524519679706 26.459260424754667 30.005780603850774 21.605434451714856 24-25 21.850775980975236 26.73480853378119 30.13768328483363 21.276732200409946 26-27 21.607849408207365 26.621903467808206 30.500430557895285 21.26981656608914 28-29 21.179499721231235 27.146519495000977 30.37630362878262 21.297677154985173 30-31 21.485341050190357 26.671387084526266 30.220936079385112 21.622335785898265 32-33 21.407730959067166 26.853897803028488 30.197754799782455 21.540616438121884 34-35 22.0412893073693 26.716097527601747 29.95236506999855 21.290248095030403 36-37 21.943598604078282 26.482739154235663 30.211013411796905 21.362648829889146 38-39 21.596647280191583 27.026171741361615 30.063291139240505 21.313889839206297 40-41 21.564081358982012 27.011589113707757 30.005806957573288 21.418522569736943 42-43 21.397466553096 27.073690944107497 29.53282089700303 21.996021605793473 44-45 21.606857604148495 27.05252662076231 29.400079357749526 21.94053641733967 46-47 21.962167279083232 27.401572244488893 28.949873055751997 21.686387420675878 48-49 21.963698119403187 27.273527983198452 28.902973792046627 21.85980010535173 50-51 21.610479653300587 27.585879592416124 29.084218951487394 21.719421802795896 52-53 21.897067450184963 27.942597479447183 28.394785133903298 21.765549936464556 54-55 21.46981259213906 27.652578867617333 28.645457437311233 22.232151102932374 56-57 21.8586351618056 27.771953467849237 28.437150470144378 21.93226090020078 58-59 22.14993295569603 27.386229920915085 28.520595599704457 21.943241523684424 60-61 21.817920592757805 27.64766350184809 29.03360431842944 21.500811586964666 62-63 21.62199604232692 27.544908643428023 29.040324139240308 21.792771175004745 64-65 21.384983752351634 27.863434239781082 28.695826919787926 22.05575508807936 66-67 21.606298081363327 27.6818668481886 28.3571865924616 22.354648477986476 68-69 21.458782643199083 27.656821614077813 28.53813881814901 22.346256924574092 70-71 21.71663705099767 27.62740010911336 28.377853750472454 22.278109089416517 72-73 21.66033308591893 27.386038302486345 28.6299797849889 22.323648826605826 74-75 21.402685757481436 28.12024340104735 28.07629028892758 22.400780552543637 76-77 21.505294326864156 27.542498168090056 28.393104305309752 22.559103199736033 78-79 21.49991919091723 27.633542353793285 28.511924256663328 22.35461419862616 80-81 21.595472488079192 27.74999486909347 28.168762356933225 22.48577028589411 82-83 21.54163734298827 27.647575419930753 28.355790354013404 22.454996883067572 84-85 21.403198828829137 27.717225857427735 28.2362830375192 22.64329227622393 86-87 21.32794903119374 27.66686043077271 28.313901276189192 22.691289261844368 88-89 21.405931054263657 27.555537617187397 28.04682944590766 22.99170188264128 90-91 21.348071497382126 27.37853807807426 28.263755209558628 23.009635214984982 92-93 21.483152447179304 27.540130730579392 28.1893370092045 22.7873798130368 94-95 21.241239306737995 27.400062252656852 28.249024309320454 23.109674131284695 96-97 21.08006741763274 27.24402634794647 28.431530997659536 23.244375236761254 98-99 21.064680011937714 27.382604628173524 28.01284074010414 23.539874619784626 100-101 21.116023327803525 26.622513724752437 28.63342511672453 23.628037830719503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 437.0 1 296.5 2 338.5 3 480.0 4 329.0 5 231.0 6 339.0 7 663.0 8 1038.0 9 1152.5 10 1005.0 11 846.5 12 833.0 13 935.0 14 1017.0 15 1076.5 16 1120.5 17 1155.5 18 1214.0 19 1274.5 20 1401.0 21 1587.5 22 1881.5 23 2222.5 24 2603.0 25 3118.5 26 3885.5 27 4717.0 28 5325.0 29 6068.5 30 7113.0 31 8363.0 32 9797.5 33 10997.0 34 12301.0 35 13514.0 36 14893.0 37 16366.0 38 16950.0 39 17405.5 40 17578.5 41 17857.5 42 18412.0 43 19102.0 44 19931.5 45 20552.5 46 20643.0 47 21873.0 48 25736.5 49 25430.5 50 21755.5 51 19909.5 52 18612.0 53 18003.0 54 17418.5 55 16265.5 56 14539.5 57 12850.0 58 11748.5 59 10433.5 60 8657.5 61 7099.5 62 5871.5 63 4558.0 64 3369.5 65 2550.5 66 1921.0 67 1538.5 68 1191.0 69 847.0 70 612.0 71 447.0 72 357.0 73 298.5 74 221.0 75 171.5 76 122.0 77 58.5 78 34.0 79 28.5 80 13.5 81 6.0 82 3.5 83 3.0 84 2.5 85 1.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34067937487386996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.55118912836019E-4 16-17 0.0022233091733736493 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.275594564180095E-4 26-27 0.0012826783692540284 28-29 0.0 30-31 0.003420475651344076 32-33 0.0 34-35 0.010090403171465024 36-37 0.026337662515349386 38-39 0.019496711212661232 40-41 0.012057176670987867 42-43 0.009577331823763413 44-45 0.003420475651344076 46-47 0.0014537021518212323 48-49 0.0010261426954032227 50-51 0.0 52-53 1.710237825672038E-4 54-55 0.0 56-57 0.0 58-59 0.004446618346747299 60-61 0.009748355606330616 62-63 0.004617642129314503 64-65 0.0023943329559408533 66-67 0.0047886659118817065 68-69 0.00359149943391128 70-71 1.710237825672038E-4 72-73 0.0 74-75 0.0 76-77 0.22344257192405179 78-79 4.275594564180095E-4 80-81 0.0037625232164784834 82-83 7.696070215524171E-4 84-85 0.0 86-87 0.11467144621131016 88-89 0.42302732617997857 90-91 0.4054973884668402 92-93 0.0 94-95 0.0 96-97 8.55118912836019E-5 98-99 0.0021377972820900474 100-101 6.840951302688152E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 584714.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.3388428187402 #Duplication Level Percentage of deduplicated Percentage of total 1 86.18052609137327 60.618384787774424 2 8.714536657028336 12.259408483137316 3 2.157734859037315 4.553177192830267 4 0.9218343189281575 2.5936303705603234 5 0.48432393645293764 1.7033392619758347 6 0.3233546564332175 1.3646635412138293 7 0.21971419282942106 1.0818109452132514 8 0.1680459348117727 0.9456125276042834 9 0.11593712438120998 0.7339394851835994 >10 0.6631375478033665 8.259463706408608 >50 0.035038646155756484 1.6998736223672337 >100 0.013139475035721888 1.830631233180394 >500 0.00145994167063146 0.6466085264533237 >1k 0.0012166180588595499 1.7094563160973524 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.48827289922936684 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2364 0.4043002219888698 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2072 0.3543612774792463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1583 0.2707306478038836 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1114 0.19052049377986505 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 746 0.12758374179513404 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 0.11629617214569858 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 640 0.10945522084301043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 602 0.10295631710545668 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.420475651344076E-4 2 0.0 0.0 0.0 0.0 3.420475651344076E-4 3 0.0 0.0 0.0 0.0 3.420475651344076E-4 4 0.0 0.0 0.0 0.0 3.420475651344076E-4 5 0.0 0.0 0.0 0.0 3.420475651344076E-4 6 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 7 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 8 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 9 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 10-11 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 12-13 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 14-15 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 16-17 1.710237825672038E-4 0.0 0.0 0.0 3.420475651344076E-4 18-19 2.565356738508057E-4 0.0 0.0 1.710237825672038E-4 3.420475651344076E-4 20-21 3.420475651344076E-4 0.0 0.0 1.710237825672038E-4 3.420475651344076E-4 22-23 3.420475651344076E-4 0.0 0.0 0.0010261426954032227 3.420475651344076E-4 24-25 3.420475651344076E-4 0.0 0.0 0.001624725934388436 3.420475651344076E-4 26-27 3.420475651344076E-4 0.0 0.0 0.002308821064657251 3.420475651344076E-4 28-29 3.420475651344076E-4 0.0 0.0 0.004361106455463696 3.420475651344076E-4 30-31 3.420475651344076E-4 0.0 0.0 0.01077449830173384 3.420475651344076E-4 32-33 3.420475651344076E-4 0.0 0.0 0.022404115516303696 3.420475651344076E-4 34-35 3.420475651344076E-4 0.0 0.0 0.04019058890329289 3.420475651344076E-4 36-37 3.420475651344076E-4 0.0 0.0 0.06721234654891109 3.420475651344076E-4 38-39 3.420475651344076E-4 0.0 0.0 0.10936970895172683 3.420475651344076E-4 40-41 3.420475651344076E-4 0.0 0.0 0.1484486432683329 3.420475651344076E-4 42-43 5.130713477016114E-4 0.0 0.0 0.19633530238714997 3.420475651344076E-4 44-45 6.840951302688152E-4 0.0 0.0 0.2536282695471632 3.420475651344076E-4 46-47 7.69607021552417E-4 0.0 0.0 0.32810912685518046 3.420475651344076E-4 48-49 9.406308041196208E-4 0.0 0.0 0.4033595911847502 3.420475651344076E-4 50-51 0.0010261426954032227 0.0 0.0 0.49160786298942727 3.420475651344076E-4 52-53 0.0010261426954032227 0.0 0.0 0.5759225877950588 3.420475651344076E-4 54-55 0.0010261426954032227 0.0 0.0 0.6779382740963958 3.420475651344076E-4 56-57 0.0010261426954032227 0.0 0.0 0.8003057905232301 3.420475651344076E-4 58-59 0.0010261426954032227 0.0 0.0 0.9329347339040968 3.420475651344076E-4 60-61 0.0010261426954032227 0.0 0.0 1.0757395923477118 3.420475651344076E-4 62-63 0.0010261426954032227 0.0 0.0 1.2242737475073282 3.420475651344076E-4 64-65 0.0010261426954032227 0.0 0.0 1.386062245815903 3.420475651344076E-4 66-67 0.0011971664779704266 0.0 0.0 1.5784640012040074 3.420475651344076E-4 68-69 0.0011971664779704266 0.0 0.0 1.7958352288469235 3.420475651344076E-4 70-71 0.0013681902605376303 0.0 0.0 2.0341568698543218 3.420475651344076E-4 72-73 0.0013681902605376303 0.0 0.0 2.2888112820968884 3.420475651344076E-4 74-75 0.0013681902605376303 0.0 0.0 2.5503066456421433 3.420475651344076E-4 76-77 0.0013681902605376303 0.0 0.0 2.8328379344431633 3.420475651344076E-4 78-79 0.0013681902605376303 0.0 0.0 3.1617166683198965 3.420475651344076E-4 80-81 0.001453702151821232 0.0 0.0 3.509664553952873 3.420475651344076E-4 82-83 0.001710237825672038 0.0 0.0 3.867018747627045 3.420475651344076E-4 84-85 0.001710237825672038 0.0 0.0 4.23061530936492 3.420475651344076E-4 86-87 0.001710237825672038 0.0 0.0 4.63183710326758 3.420475651344076E-4 88-89 0.001710237825672038 0.0 0.0 5.064783808836457 3.420475651344076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 685 0.0 51.942604 2 GTACCTG 855 0.0 41.7505 1 TAACGCA 65 1.3371429E-4 36.493004 4 ACCTGGG 1075 0.0 32.656998 3 ATAACGC 80 4.5238275E-4 29.650568 3 TATAACG 85 6.450163E-4 27.906416 2 TTCAACG 70 0.008439343 27.10909 2 CGATAAT 80 6.317714E-8 26.68551 48-49 TACATGG 3410 0.0 23.51177 2 GTACATG 3445 0.0 23.48693 1 AGTACTT 2835 0.0 22.509163 12-13 GTATAAC 235 4.5311026E-9 22.278778 1 ACATGGG 3455 0.0 22.244364 3 GTACAAC 110 0.0028423371 21.634352 1 GTTCGTC 55 0.0028753178 21.593596 80-81 CATGGGA 2000 0.0 21.585613 4 GTAGCAC 155 3.9398343E-5 21.424927 3 GTAGGGT 200 5.441525E-7 21.34841 3 GAGTACT 2815 0.0 20.899382 12-13 GTATCAA 5795 0.0 20.779428 1 >>END_MODULE