FastQCFastQC Report
Fri 27 May 2016
SRR939224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577174
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.5218530287227076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.4024782821124999No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20900.36210917331688536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.26283235211565315No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10590.18348019834573281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.12561203380609662No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7060.12232013223048856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.11417700728030022No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6440.1115781376153465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5830.10100940097786802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6550.039.042222
GTACACG1255.664333E-934.1858181
GTACCTG7600.033.7360041
ACCTGGG9500.026.420093
GTACATG34450.024.6704641
GTACTAG1351.3455279E-524.6194171
TACATGG35800.023.2815362
GAGTACT26050.023.25724812-13
ATGGGAC3350.022.6181555
AGGACCG1050.00223376322.5508425
ACATGGG36150.022.5321273
CATGGGA19250.022.3868354
CTAGGTA1100.002926478621.5258034
GTATCAA59600.020.8722151
GTACTTT29000.020.56499714-15
GTATTAG2108.626612E-720.3487031
GGTATCA46350.020.282851
TACACGG2351.1659722E-720.1518152
TAGACCC1656.4641616E-520.0907484
ATAAGCC1708.131583E-519.4998463
Produced by FastQC (version 0.11.5)