##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.147598471171605 33.0 31.0 34.0 31.0 34.0 2 32.23331958820044 34.0 31.0 34.0 30.0 34.0 3 32.2900737039437 34.0 31.0 34.0 30.0 34.0 4 35.729733148062806 37.0 35.0 37.0 35.0 37.0 5 35.77345653130598 37.0 35.0 37.0 35.0 37.0 6 35.86431474737254 37.0 37.0 37.0 35.0 37.0 7 35.80415784494797 37.0 37.0 37.0 35.0 37.0 8 35.80295370200321 37.0 37.0 37.0 35.0 37.0 9 37.486906894627964 39.0 38.0 39.0 35.0 39.0 10-11 37.3898720316577 39.0 38.0 39.0 35.0 39.0 12-13 37.30153125400659 39.0 37.5 39.0 35.0 39.0 14-15 38.57735102412791 40.0 38.0 41.0 34.0 41.0 16-17 38.50142071541684 40.0 38.0 41.0 34.0 41.0 18-19 38.41899756399283 40.0 38.0 41.0 34.0 41.0 20-21 38.4056670951914 40.0 38.0 41.0 34.0 41.0 22-23 38.43919771160863 40.0 38.0 41.0 34.0 41.0 24-25 38.38682269125082 40.0 38.0 41.0 34.0 41.0 26-27 38.26402696587164 40.0 38.0 41.0 34.0 41.0 28-29 38.12642114856179 40.0 38.0 41.0 34.0 41.0 30-31 37.906801068655206 40.0 38.0 41.0 33.0 41.0 32-33 37.66452577558934 40.0 37.5 41.0 33.0 41.0 34-35 37.34296330049517 40.0 37.0 41.0 32.5 41.0 36-37 37.065835432642494 40.0 37.0 41.0 31.0 41.0 38-39 36.88371097797198 40.0 36.5 41.0 31.0 41.0 40-41 36.69597642998472 40.0 36.0 41.0 30.0 41.0 42-43 36.46716847952264 40.0 36.0 41.0 30.0 41.0 44-45 36.14780897961447 40.0 35.5 41.0 29.5 41.0 46-47 35.91803251705724 40.0 36.0 41.0 28.0 41.0 48-49 35.74710226032357 40.0 36.0 41.0 27.0 41.0 50-51 34.61724020832539 38.5 34.0 40.0 25.0 40.5 52-53 34.75989476310437 38.5 34.5 39.5 25.0 40.5 54-55 35.28007585234262 39.0 35.0 41.0 26.0 41.0 56-57 35.200861438664944 39.0 35.0 41.0 25.5 41.0 58-59 35.00257894499752 39.0 35.0 41.0 25.0 41.0 60-61 34.707455637294814 39.0 35.0 41.0 24.0 41.0 62-63 34.34789768769903 38.0 34.5 40.0 23.0 41.0 64-65 33.99031574533849 37.5 34.0 40.0 22.0 41.0 66-67 33.607857422545024 37.0 34.0 40.0 21.0 41.0 68-69 33.19328140214216 36.5 33.5 39.0 19.5 41.0 70-71 32.76939709688933 36.0 33.0 39.0 18.0 41.0 72-73 32.32930710669573 35.0 33.0 38.5 14.5 40.0 74-75 31.888556137317345 35.0 32.0 37.5 10.0 40.0 76-77 31.456145806983685 35.0 32.0 37.0 7.5 39.0 78-79 31.017019131145894 35.0 31.5 36.5 4.5 39.0 80-81 30.571921119107927 35.0 31.0 36.0 2.0 38.5 82-83 30.174704681777072 35.0 31.0 36.0 2.0 37.0 84-85 29.792881349471735 34.5 30.5 35.0 2.0 37.0 86-87 29.39377899905401 34.0 30.0 35.0 2.0 36.5 88-89 28.978395596475238 34.0 29.5 35.0 2.0 36.0 90-91 28.572820328012003 34.0 29.0 35.0 2.0 36.0 92-93 28.175552779577735 34.0 29.0 35.0 2.0 35.0 94-95 27.717731567950047 34.0 28.0 35.0 2.0 35.0 96-97 27.128712485316385 34.0 26.5 35.0 2.0 35.0 98-99 26.451045958411157 34.0 25.0 35.0 2.0 35.0 100-101 23.58499170094287 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1537691046594034 1101 2 0.3289634680219251 1101 3 0.24830092314839192 1101 4 0.15841400612104195 1101 5 -0.017790621803563056 1101 6 0.07739293924893786 1101 7 -0.016114695175915017 1101 8 -0.06018895062816654 1101 9 0.05728394036447071 1101 10-11 0.22013664877227512 1101 12-13 0.036480810877286274 1101 14-15 0.20980462498800279 1101 16-17 0.17711399928502658 1101 18-19 0.23634541859838976 1101 20-21 0.1275590435480538 1101 22-23 0.17838911030670346 1101 24-25 0.3334978003745377 1101 26-27 0.03125940840639174 1101 28-29 0.1382478859376306 1101 30-31 0.2512921705493554 1101 32-33 0.27435114195080956 1101 34-35 0.19222467850386948 1101 36-37 0.029373238026899173 1101 38-39 0.13678267534934463 1101 40-41 0.2392019561306924 1101 42-43 -0.060614246274290906 1101 44-45 0.07925188521105042 1101 46-47 0.8720343744861623 1101 48-49 0.715654698554232 1101 50-51 0.5102547818706213 1101 52-53 0.6207288877633417 1101 54-55 0.4712190614961358 1101 56-57 0.6202981651633976 1101 58-59 0.509334869931422 1101 60-61 -0.02366634887704322 1101 62-63 0.1073490097008829 1101 64-65 0.08975035401956433 1101 66-67 0.2759980054141167 1101 68-69 5.42171435078842E-4 1101 70-71 -0.1277207804395033 1101 72-73 -0.28809216825826667 1101 74-75 -0.17921351786691986 1101 76-77 0.07883433602020773 1101 78-79 0.13389348585199556 1101 80-81 0.020146389396526132 1101 82-83 -0.09712781889787436 1101 84-85 -0.1449594186781873 1101 86-87 -0.11680506844604821 1101 88-89 -0.06826300158549259 1101 90-91 -0.09052402709646756 1101 92-93 -0.1434392410937697 1101 94-95 -0.12237677803084779 1101 96-97 -0.09592999355350074 1101 98-99 -0.3508708761950601 1101 100-101 -0.024259556874476118 1102 1 0.11921834462276593 1102 2 0.2144599568879073 1102 3 0.11408200656973122 1102 4 -0.0705429643433888 1102 5 0.019472945989100765 1102 6 -0.06149581060716969 1102 7 0.038326651618014296 1102 8 -0.07815754812658327 1102 9 0.0827903394875591 1102 10-11 0.017048953007162027 1102 12-13 0.10330497388600435 1102 14-15 0.1676534985721574 1102 16-17 0.16550948861647896 1102 18-19 0.025204387631340808 1102 20-21 0.08100335841394468 1102 22-23 0.1710515158964867 1102 24-25 0.1690475944113743 1102 26-27 0.08520023716415892 1102 28-29 0.0388274858235107 1102 30-31 0.2038630814421296 1102 32-33 0.1153465722211422 1102 34-35 0.20953008960436392 1102 36-37 0.08413992154434169 1102 38-39 0.12108893232530704 1102 40-41 0.34518414769897277 1102 42-43 0.19729268740206152 1102 44-45 0.33008399644617725 1102 46-47 0.49618787335749204 1102 48-49 0.32289319039328745 1102 50-51 0.06600998850949225 1102 52-53 0.14097734491649305 1102 54-55 0.10457458446240508 1102 56-57 0.11824604121510873 1102 58-59 -0.004649603999091312 1102 60-61 0.2540861249333517 1102 62-63 0.32177844416555246 1102 64-65 0.2307437822177505 1102 66-67 0.10636743777661195 1102 68-69 -0.18407140361509988 1102 70-71 -0.28918039113657557 1102 72-73 -0.29999949233869216 1102 74-75 -0.21726540156655005 1102 76-77 -0.3693995860891768 1102 78-79 -0.18928850751113657 1102 80-81 0.05380122214842231 1102 82-83 -0.299234499312103 1102 84-85 -0.3661658673525672 1102 86-87 -0.47020583824278717 1102 88-89 -0.6025184559807926 1102 90-91 -0.5560958939786893 1102 92-93 -0.6475860330234156 1102 94-95 -0.8551777270130465 1102 96-97 -1.0784443959599912 1102 98-99 -0.8775234616209744 1102 100-101 -0.8462603276722511 1103 1 0.040371716995650786 1103 2 0.12916990818322205 1103 3 0.2458120651290585 1103 4 0.13985368507764662 1103 5 0.26777207233701006 1103 6 0.10758240115396944 1103 7 0.17374272605847807 1103 8 0.27725871582627093 1103 9 0.2494806952023083 1103 10-11 0.3517301931762091 1103 12-13 0.2662998381003874 1103 14-15 0.2144109416710691 1103 16-17 0.3428883428246081 1103 18-19 0.1660689431300426 1103 20-21 0.1402686188987161 1103 22-23 0.26473222543085484 1103 24-25 0.08398496056825167 1103 26-27 0.26875482620655333 1103 28-29 0.34912795770934224 1103 30-31 0.341366161018378 1103 32-33 0.12457108754322377 1103 34-35 0.3606591096358187 1103 36-37 0.2642809187585087 1103 38-39 0.2985222713440123 1103 40-41 0.4815762109203021 1103 42-43 -0.002948467107387387 1103 44-45 -0.15134852766927054 1103 46-47 0.37759812976774754 1103 48-49 0.3899639922201885 1103 50-51 0.34522806041049137 1103 52-53 0.07794078797750359 1103 54-55 0.3016366687196097 1103 56-57 0.1402934382625105 1103 58-59 0.3545071606454755 1103 60-61 0.034202011756548245 1103 62-63 -0.08998222064388983 1103 64-65 0.15631042424781327 1103 66-67 -0.18970302292799346 1103 68-69 -0.2804455579160816 1103 70-71 -0.22199351297408754 1103 72-73 -0.0608632897878536 1103 74-75 -0.19671752126257047 1103 76-77 -0.15189148443473144 1103 78-79 -0.25055549255860754 1103 80-81 -0.21931455096735064 1103 82-83 -0.2959132947713847 1103 84-85 -0.38737467209795895 1103 86-87 -0.6786623546261339 1103 88-89 -0.4972774909837341 1103 90-91 -0.3649158003595723 1103 92-93 -0.572193268606263 1103 94-95 -0.5542225845944877 1103 96-97 -0.7389187454587294 1103 98-99 -0.9399488411195236 1103 100-101 -1.1156213866674562 1104 1 0.0021599867171104847 1104 2 0.19141675435491123 1104 3 0.27878674528536607 1104 4 0.3323844204058801 1104 5 0.2893626367250661 1104 6 0.38350000014046515 1104 7 0.3408554787662226 1104 8 0.2958292777162441 1104 9 0.3720339132670034 1104 10-11 0.3873256288238309 1104 12-13 0.37075980633905914 1104 14-15 0.3751580465726576 1104 16-17 0.059574637277080456 1104 18-19 0.18674057027348567 1104 20-21 0.3859415843002836 1104 22-23 0.5074253360780361 1104 24-25 0.41647965214436056 1104 26-27 0.3360045273288961 1104 28-29 0.2999938107150797 1104 30-31 0.4146380236952538 1104 32-33 0.47643929588324596 1104 34-35 0.604273387957825 1104 36-37 0.7353210129718804 1104 38-39 0.11778382051161174 1104 40-41 0.36934988470650154 1104 42-43 0.6678244215673814 1104 44-45 0.6989048492020586 1104 46-47 0.7790688754451764 1104 48-49 0.840687097692765 1104 50-51 0.9923115498739392 1104 52-53 0.8197774134258111 1104 54-55 0.7288287355254539 1104 56-57 0.7681796149482736 1104 58-59 0.8749267491757067 1104 60-61 0.8024268569647006 1104 62-63 0.5884232001936809 1104 64-65 0.7993456828884931 1104 66-67 0.8568638988998885 1104 68-69 0.4130197276682068 1104 70-71 0.21480055586591362 1104 72-73 0.2877618730335527 1104 74-75 0.5337795970216028 1104 76-77 0.5043233862895313 1104 78-79 0.8715806350576862 1104 80-81 0.8735962496678766 1104 82-83 0.5491654840234688 1104 84-85 0.506004072794358 1104 86-87 0.19796139856338257 1104 88-89 0.45987594477095683 1104 90-91 0.16025237407791337 1104 92-93 0.023228996440220584 1104 94-95 -0.19851357544080273 1104 96-97 -0.04324310354091665 1104 98-99 0.038291081796806736 1104 100-101 -0.08219392917945711 1105 1 0.01224490799234701 1105 2 0.019744362502699175 1105 3 0.02234695122569974 1105 4 0.171283521288359 1105 5 0.2059509806354356 1105 6 0.01496994499569837 1105 7 0.060486349853896115 1105 8 0.03141303870569345 1105 9 -0.048617510266417696 1105 10-11 0.15826899523293037 1105 12-13 -0.1390360831717956 1105 14-15 -0.06312847331854954 1105 16-17 0.14168461074437744 1105 18-19 0.1502518695440429 1105 20-21 0.0814185490924686 1105 22-23 -2.080136129691823E-4 1105 24-25 -0.016067819763414093 1105 26-27 0.08661586070105187 1105 28-29 0.00926944305003019 1105 30-31 0.12429002113347565 1105 32-33 0.12819760710599581 1105 34-35 7.355559871697892E-4 1105 36-37 0.10389681415129104 1105 38-39 0.2209224143616879 1105 40-41 0.300352388262219 1105 42-43 0.163152087937398 1105 44-45 -0.042289039127709316 1105 46-47 0.20435520403478336 1105 48-49 0.4955374249848532 1105 50-51 0.4823888190851946 1105 52-53 0.3720464167444817 1105 54-55 0.23838965676741708 1105 56-57 0.31536664969030426 1105 58-59 0.3374940166402993 1105 60-61 0.2905058562078011 1105 62-63 0.1990454294395292 1105 64-65 0.02953972925002546 1105 66-67 0.12492734449121912 1105 68-69 0.11112916881801027 1105 70-71 -0.17796450081758763 1105 72-73 0.19352088292301772 1105 74-75 0.15394819195293863 1105 76-77 0.17518482570930516 1105 78-79 0.19414008410908323 1105 80-81 0.18536587263737303 1105 82-83 0.22001444725197317 1105 84-85 0.017744168777856117 1105 86-87 -0.19066988007987362 1105 88-89 0.17488854572413715 1105 90-91 0.44174643956960225 1105 92-93 0.24255628020702957 1105 94-95 -0.0742195199300717 1105 96-97 0.1809062281511018 1105 98-99 0.08433862440299933 1105 100-101 -0.1255091858978865 1106 1 -0.0600433767272861 1106 2 -0.2014587790420208 1106 3 -0.25901707641136085 1106 4 -0.10849983096228755 1106 5 -0.05591932018803902 1106 6 -0.08204924402260616 1106 7 -0.00936426679994895 1106 8 -0.029669590624024522 1106 9 -0.08605475935800655 1106 10-11 -0.16499816556936509 1106 12-13 -0.13870661709727727 1106 14-15 -0.20243548999371797 1106 16-17 -0.22697078678595517 1106 18-19 -0.10542523084978939 1106 20-21 -0.012911587340759922 1106 22-23 -0.1764723634792844 1106 24-25 0.17160405487950214 1106 26-27 0.046122004386347726 1106 28-29 -0.13591273454932207 1106 30-31 0.15491513063809492 1106 32-33 -0.18857545361479566 1106 34-35 -0.279003994415433 1106 36-37 -0.2555259155798737 1106 38-39 -0.1562933878215489 1106 40-41 -0.06007207068973486 1106 42-43 0.03816256229236359 1106 44-45 0.05679074117745131 1106 46-47 0.05163720414143569 1106 48-49 0.2216692266500857 1106 50-51 -0.002257680956709862 1106 52-53 -0.07368690236911846 1106 54-55 -0.1876080731541947 1106 56-57 -0.29414749259333206 1106 58-59 -0.5605886050717075 1106 60-61 -0.34362111582757393 1106 62-63 -0.34661514051773423 1106 64-65 -0.31009599172839586 1106 66-67 -0.30628470977737265 1106 68-69 -0.14306628645459085 1106 70-71 -0.24852927803757296 1106 72-73 -0.21199662638964156 1106 74-75 -0.16987125302050998 1106 76-77 -0.019702927293977268 1106 78-79 -0.13580209745148508 1106 80-81 0.008259765517099282 1106 82-83 0.2288738471285683 1106 84-85 0.08517235279132152 1106 86-87 -0.2063604638251988 1106 88-89 -0.21643145153348797 1106 90-91 -0.39210934189345537 1106 92-93 -0.12757314473183712 1106 94-95 0.06513320302133607 1106 96-97 -0.26541365484082746 1106 98-99 -0.20976793216232892 1106 100-101 -0.06197174864879429 1107 1 -0.18998237335843982 1107 2 -0.11021959120628111 1107 3 -0.13352859421159735 1107 4 -0.14141615619219294 1107 5 -0.06443793987300239 1107 6 -0.17269842912686073 1107 7 -0.09817424585849466 1107 8 -0.2060257774582226 1107 9 0.08377861950022947 1107 10-11 0.01785351429952442 1107 12-13 -0.15083080903026058 1107 14-15 -0.14586988860975936 1107 16-17 -0.19768552774927173 1107 18-19 -0.22494448788339128 1107 20-21 -0.26404151541141374 1107 22-23 0.020446511145138402 1107 24-25 -0.1278587512754612 1107 26-27 -0.37711208466036084 1107 28-29 -0.2607438371624511 1107 30-31 -0.44918939453717144 1107 32-33 -0.2866743337022015 1107 34-35 -0.25563515165844564 1107 36-37 -0.4293454548678639 1107 38-39 -0.6087854209636774 1107 40-41 -0.6206948513507555 1107 42-43 -0.35253943169835367 1107 44-45 -0.1328259391466844 1107 46-47 -0.22857403140441335 1107 48-49 -0.7174658002096095 1107 50-51 -0.3285692533868243 1107 52-53 -0.3364548164181045 1107 54-55 -0.36336333128038945 1107 56-57 -0.5930093700402992 1107 58-59 -0.4776112764729632 1107 60-61 -0.585132542578009 1107 62-63 -0.542882098543771 1107 64-65 -0.5490070360696464 1107 66-67 -0.7095442927692659 1107 68-69 -0.693833836304357 1107 70-71 -0.7667920477726184 1107 72-73 -0.7048437537683157 1107 74-75 -0.5945075090525584 1107 76-77 -0.8060673086105545 1107 78-79 -0.6487056757087899 1107 80-81 -0.6635147951675968 1107 82-83 -0.8050585939166837 1107 84-85 -0.5658301055533919 1107 86-87 -0.9576306085943891 1107 88-89 -0.36322376693000535 1107 90-91 -0.2995189749627478 1107 92-93 -0.7449527313657249 1107 94-95 -0.7522445626164647 1107 96-97 -0.708869905409891 1107 98-99 -0.8574579986286821 1107 100-101 -0.7974101534562266 1108 1 0.04264883971072919 1108 2 0.08094481616250704 1108 3 0.20178249372722945 1108 4 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-1.1688794421132762 2316 84-85 -1.2708683253597464 2316 86-87 -1.4278531328002622 2316 88-89 -1.498225182478393 2316 90-91 -1.6162700083130659 2316 92-93 -1.3662901414784052 2316 94-95 -1.2471248072232264 2316 96-97 -1.7172692032978922 2316 98-99 -1.732869356988914 2316 100-101 -1.5788811913363006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2145.0 3 525.0 4 573.0 5 563.0 6 632.0 7 782.0 8 931.0 9 1149.0 10 1393.0 11 1845.0 12 2582.0 13 4008.0 14 3320.0 15 3330.0 16 4431.0 17 6547.0 18 5348.0 19 3412.0 20 3188.0 21 3261.0 22 3496.0 23 3769.0 24 4274.0 25 4629.0 26 5341.0 27 6236.0 28 7200.0 29 8799.0 30 10358.0 31 12660.0 32 16027.0 33 20923.0 34 29358.0 35 44379.0 36 71676.0 37 121563.0 38 129897.0 39 26600.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.78301256627562 19.15504668865417 14.276395441072099 21.78554530399811 2 15.115879679422683 21.035978211844707 41.20129757485342 22.646844533879193 3 19.660994603183966 26.44994977890575 29.68665159744714 24.202404020463145 4 12.579612585337927 16.934167266670716 37.79574416405701 32.69047598393435 5 13.177878985035784 38.03617436564736 33.78061158100195 15.0053350683149 6 29.85017174503708 36.240543619424784 18.77283359371072 15.136451041827414 7 27.743144847342478 33.445584489225446 21.32731500668478 17.483955656747295 8 25.89887513807173 34.445016672649054 20.367335826861087 19.288772362418133 9 26.678920399864747 17.685431640641934 21.27034220861981 34.36530575087351 10-11 24.821832961682382 26.89021789265241 27.28938141791485 20.998567727750363 12-13 26.22889985772116 24.678572078840336 27.899657438339336 21.192870625099168 14-15 23.010786062129313 25.81138219558483 27.13664339411132 24.041188348174536 16-17 21.401001198177937 29.00275528906259 27.720306081576666 21.8759374311828 18-19 21.359051572028207 27.51381014272721 30.36679357575831 20.760344709486276 20-21 22.491828011063618 26.39409362454805 30.103092783505154 21.010985580883183 22-23 21.9355241513449 26.479894763039074 30.004153634169757 21.580427451446273 24-25 21.93600815942801 26.5850087856086 30.23142663366354 21.247556421299855 26-27 21.70232362899645 26.472128940941385 30.63492173623446 21.19062569382771 28-29 21.249696465119506 27.056145280466232 30.526242758524997 21.167915495889268 30-31 21.590578000998946 26.68459334591265 30.245331316943226 21.47949733614518 32-33 21.43378033288677 26.840177065584502 30.244657466540065 21.481385134988663 34-35 22.039948085049417 26.795573146444145 29.964106357842834 21.2003724106636 36-37 21.969100467900233 26.416939869768346 30.28817846404694 21.32578119828448 38-39 21.643589877562395 26.964923280561504 30.15518052144835 21.236306320427747 40-41 21.691850843278885 26.9203279267954 30.065702015633722 21.322119214291995 42-43 21.481107987696767 27.04403665889877 29.5524925194807 21.922362833923764 44-45 21.720878868215056 26.90288485321424 29.49249436511282 21.88374191345789 46-47 22.046183925764325 27.380370742922832 29.027159074579682 21.54628625673316 48-49 22.03056357802544 27.29061353604537 28.96859682066325 21.710226065265935 50-51 21.677436099766844 27.594745317931256 29.092188611836185 21.63562997046571 52-53 21.956508864401997 27.866690820667 28.518064985873394 21.65873532905761 54-55 21.54905818286641 27.566690093437106 28.733151667001955 22.151100056694524 56-57 21.94235835537439 27.66821578006188 28.529862947865865 21.859562916697865 58-59 22.149127388712465 27.321943423031925 28.64191598417739 21.887013204078222 60-61 21.924228740666496 27.5125853305422 29.050057684577595 21.513128244213707 62-63 21.666997554910033 27.644929050594552 29.07889420632101 21.6091791881744 64-65 21.472107833915146 27.772406753206937 28.83970191684609 21.91578349603183 66-67 21.636721887244097 27.691066740301846 28.37294708545804 22.299264286996014 68-69 21.539712049958272 27.624629956492118 28.67686903552592 22.158788958023692 70-71 21.865289307315898 27.568414239256654 28.422062258199126 22.144234195228318 72-73 21.866948191429874 27.27166617016569 28.723191125001524 22.13819451340291 74-75 21.493032144991314 28.06628303829489 28.311233760690463 22.12945105602333 76-77 21.69557534763135 27.5063116633617 28.371525537090115 22.426587451916838 78-79 21.707227035912293 27.681233109519965 28.47229961273786 22.13924024182988 80-81 21.881630787314162 27.745238458022246 28.267125225833134 22.106005528830455 82-83 21.92064733328573 27.62452300795408 28.369597210587088 22.085232448173105 84-85 21.706968765510577 27.610957758988512 28.338049999564614 22.344023475936297 86-87 21.76960788582276 27.544126995184566 28.39810517332962 22.288159945663057 88-89 21.74849054089713 27.4650579703708 28.16054566086571 22.62590582786636 90-91 21.774479445671158 27.42481849737164 28.2683320921036 22.5323699648536 92-93 21.948157949523818 27.480624780594205 28.291435355975285 22.27978191390669 94-95 21.848242485902084 27.276615315467552 28.38528555575493 22.489856642875434 96-97 21.693506520656 27.13850688649564 28.599513607492394 22.56847298535596 98-99 21.74055065434628 27.312859751083607 28.079138269849896 22.86745132472021 100-101 22.015945379889317 26.4914503677191 28.6216521015941 22.870952150797486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 873.0 1 529.0 2 399.5 3 503.5 4 316.5 5 258.5 6 384.5 7 696.0 8 1101.5 9 1314.5 10 1175.0 11 1017.0 12 1030.0 13 1119.5 14 1204.5 15 1251.5 16 1310.5 17 1347.0 18 1369.0 19 1414.0 20 1530.5 21 1733.5 22 1961.5 23 2232.0 24 2593.5 25 3069.0 26 3770.0 27 4629.5 28 5376.5 29 6126.5 30 7077.5 31 8232.5 32 9471.0 33 10712.0 34 12167.5 35 13328.0 36 14544.5 37 16012.5 38 16777.0 39 17103.0 40 17157.5 41 17430.0 42 17982.5 43 18741.0 44 19357.0 45 19921.5 46 20251.5 47 21412.5 48 25099.0 49 24985.5 50 21322.0 51 19432.5 52 18234.0 53 17770.5 54 17187.5 55 15873.5 56 14267.0 57 12672.0 58 11450.5 59 10044.5 60 8434.0 61 6999.0 62 5686.0 63 4424.0 64 3329.5 65 2547.0 66 1996.0 67 1585.5 68 1235.0 69 894.0 70 659.5 71 509.5 72 414.0 73 339.5 74 256.5 75 186.5 76 135.0 77 98.0 78 66.0 79 40.0 80 31.5 81 21.5 82 13.0 83 10.0 84 8.5 85 6.5 86 6.5 87 3.5 88 2.5 89 2.5 90 1.5 91 2.0 92 3.0 93 2.5 94 1.0 95 1.5 96 2.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3988398645815646 2 0.12370619605179721 3 0.12526551785076945 4 0.1367005443765658 5 0.13843312415320164 6 0.07744631601562094 7 0.08506966703281853 8 0.0833370872561827 9 0.08299057130085555 10-11 0.08108473354655615 12-13 0.08480978006632316 14-15 0.07926552478108854 16-17 0.0805649596135654 18-19 0.08914122950791269 20-21 0.08818831063076299 22-23 0.09884367625707326 24-25 0.11487003919095455 26-27 0.11469678121329095 28-29 0.10984555783871068 30-31 0.09806401535758714 32-33 0.09555177468146521 34-35 0.346949100271322 36-37 0.5405648903103744 38-39 0.5204669649013989 40-41 0.49179276959807616 42-43 0.5225460606333618 44-45 0.5322485073825225 46-47 0.529563008728737 48-49 0.535713666935794 50-51 0.5363200698576166 52-53 0.5336345712038311 54-55 0.527483912996774 56-57 0.4967306219614882 58-59 0.4861618853240097 60-61 0.43262517022596303 62-63 0.5131901298395285 64-65 0.5381392786230842 66-67 0.5497475631265442 68-69 0.5639547172949578 70-71 0.5588436069538822 72-73 0.5469754354839268 74-75 0.5511336269478528 76-77 0.5264443651307925 78-79 0.5000225235370963 80-81 0.5044406019675176 82-83 0.5202937069237353 84-85 0.5144029356831735 86-87 0.5161355154598093 88-89 0.527483912996774 90-91 0.5451562267184592 92-93 0.551046997959021 94-95 0.5462824035732725 96-97 0.546109145595609 98-99 0.548534757282899 100-101 0.5243652693988294 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 577174.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.57036293675903 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09107824592851 62.33140078614782 2 8.123300604504562 11.62775145017571 3 2.014241549036177 4.324799961204567 4 0.845503745786171 2.42052039801222 5 0.48983995429293564 1.7529011654835427 6 0.30343114015227024 1.3030006096207545 7 0.2066094245877023 1.0350978052727757 8 0.16109196142368756 0.9223528116230163 9 0.11429590061519941 0.7362179180292205 >10 0.6000303274892893 7.65621209149886 >50 0.03508942425736847 1.6793218332217248 >100 0.012825769720090432 1.8116423840071376 >500 0.0014519739305710025 0.666635902106132 >1k 0.0012099782754758353 1.7321448835966293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.5218530287227076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.4024782821124999 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2090 0.36210917331688536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1517 0.26283235211565315 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1059 0.18348019834573281 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.12561203380609662 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 706 0.12232013223048856 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.11417700728030022 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 644 0.1115781376153465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 583 0.10100940097786802 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.7325797766358153E-4 0.0 0.0 0.0 0.0 3 1.7325797766358153E-4 0.0 0.0 0.0 0.0 4 1.7325797766358153E-4 0.0 0.0 0.0 0.0 5 1.7325797766358153E-4 0.0 0.0 0.0 0.0 6 1.7325797766358153E-4 0.0 0.0 0.0 0.0 7 3.4651595532716306E-4 0.0 0.0 0.0 0.0 8 3.4651595532716306E-4 0.0 0.0 0.0 0.0 9 3.4651595532716306E-4 0.0 0.0 0.0 0.0 10-11 3.4651595532716306E-4 0.0 0.0 0.0 0.0 12-13 3.4651595532716306E-4 0.0 0.0 0.0 0.0 14-15 3.4651595532716306E-4 0.0 0.0 0.0 0.0 16-17 3.4651595532716306E-4 0.0 0.0 0.0 0.0 18-19 4.331449441589538E-4 0.0 0.0 0.0 0.0 20-21 5.197739329907445E-4 0.0 0.0 0.0 0.0 22-23 5.197739329907445E-4 0.0 0.0 3.46515955327163E-4 0.0 24-25 5.197739329907445E-4 0.0 0.0 8.662898883179076E-4 0.0 26-27 5.197739329907445E-4 0.0 0.0 0.0019924667431311877 0.0 28-29 6.930319106543261E-4 0.0 0.0 0.00459133640808491 0.0 30-31 6.930319106543261E-4 0.0 0.0 0.010741994615142053 0.0 32-33 6.930319106543261E-4 0.0 0.0 0.020877586308461572 0.0 34-35 6.930319106543261E-4 0.0 0.0 0.03543125643220242 0.0 36-37 8.662898883179076E-4 0.0 0.0 0.058214680494963394 0.0 38-39 8.662898883179076E-4 0.0 0.0 0.10014311108955012 0.0 40-41 8.662898883179076E-4 0.0 0.0 0.1451035562932495 0.0 42-43 8.662898883179076E-4 0.0 0.0 0.19274950015073444 0.0 44-45 8.662898883179076E-4 0.0 0.0 0.24905834289139844 8.662898883179077E-5 46-47 8.662898883179076E-4 0.0 0.0 0.32122029058828017 1.7325797766358153E-4 48-49 8.662898883179076E-4 0.0 0.0 0.39676076884960165 1.7325797766358153E-4 50-51 8.662898883179076E-4 0.0 0.0 0.4821769518377474 1.7325797766358153E-4 52-53 8.662898883179076E-4 0.0 0.0 0.5708850364015011 1.7325797766358153E-4 54-55 8.662898883179076E-4 0.0 0.0 0.6662635531053027 1.7325797766358153E-4 56-57 8.662898883179076E-4 0.0 0.0 0.7802673024079394 1.7325797766358153E-4 58-59 0.001039547865981489 0.0 0.0 0.9152352670078694 1.7325797766358153E-4 60-61 0.001039547865981489 0.0 0.0 1.0642371277985494 1.7325797766358153E-4 62-63 0.001039547865981489 0.0 0.0 1.2117662957790891 1.7325797766358153E-4 64-65 0.001039547865981489 0.0 0.0 1.3726363280397247 1.7325797766358153E-4 66-67 0.0013860638213086523 0.0 0.0 1.5597549439163925 1.7325797766358153E-4 68-69 0.0013860638213086523 0.0 0.0 1.777366963861851 1.7325797766358153E-4 70-71 0.0013860638213086523 0.0 0.0 2.0227002602334823 1.7325797766358153E-4 72-73 0.0013860638213086523 0.0 0.0 2.273837698856844 1.7325797766358153E-4 74-75 0.0014726928101404428 0.0 0.0 2.5279205231004864 1.7325797766358153E-4 76-77 0.0015593217989722337 0.0 0.0 2.810504284669787 1.7325797766358153E-4 78-79 0.0015593217989722337 0.0 0.0 3.1338902999788623 1.7325797766358153E-4 80-81 0.0017325797766358153 0.0 0.0 3.4779806436187357 1.7325797766358153E-4 82-83 0.0017325797766358153 0.0 0.0 3.8447677823325375 1.7325797766358153E-4 84-85 0.0017325797766358153 0.0 0.0 4.222556802627977 1.7325797766358153E-4 86-87 0.0017325797766358153 8.662898883179077E-5 0.0 4.626421148561786 1.7325797766358153E-4 88-89 0.0017325797766358153 1.7325797766358153E-4 0.0 5.049863645971579 1.7325797766358153E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 655 0.0 39.04222 2 GTACACG 125 5.664333E-9 34.185818 1 GTACCTG 760 0.0 33.736004 1 ACCTGGG 950 0.0 26.42009 3 GTACATG 3445 0.0 24.670464 1 GTACTAG 135 1.3455279E-5 24.619417 1 TACATGG 3580 0.0 23.281536 2 GAGTACT 2605 0.0 23.257248 12-13 ATGGGAC 335 0.0 22.618155 5 AGGACCG 105 0.002233763 22.550842 5 ACATGGG 3615 0.0 22.532127 3 CATGGGA 1925 0.0 22.386835 4 CTAGGTA 110 0.0029264786 21.525803 4 GTATCAA 5960 0.0 20.872215 1 GTACTTT 2900 0.0 20.564997 14-15 GTATTAG 210 8.626612E-7 20.348703 1 GGTATCA 4635 0.0 20.28285 1 TACACGG 235 1.1659722E-7 20.151815 2 TAGACCC 165 6.4641616E-5 20.090748 4 ATAAGCC 170 8.131583E-5 19.499846 3 >>END_MODULE