FastQCFastQC Report
Fri 27 May 2016
SRR939224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.4723012471109232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.4059434416657715No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19790.34287753779622787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.2586741606517272No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10910.18902445363096743No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7680.13306212684563062No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.11348397536964588No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.11313745941431873No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6070.10516759244179397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7450.033.1266672
GTAAGCG452.4267934E-531.64021594-95
CTCGTAG804.406355E-429.7839741
GTACCTG8900.028.913881
TCTAGAC1503.3576725E-828.4761923
ACCTGGG9850.023.6097053
GTACATG33000.022.9607351
TACATGG32900.022.3597282
ACATGGG32550.022.0169223
GAGTACT26100.021.00437512-13
GTATCAA55600.020.7416441
ATACGGA1150.003739124420.6349229
ATAGCCC1404.8751064E-420.340143
GTATAGC2601.4317266E-820.161461
CATGGGG13700.020.092694
AGTACTT27200.020.06768412-13
TAGCCCT3353.45608E-1119.8341664
GGTATCA44350.019.6634671
GTATAGA1707.749412E-519.6223831
GTACTTT28300.019.1199514-15
Produced by FastQC (version 0.11.5)