##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939224_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51920044908468 34.0 31.0 34.0 31.0 34.0 2 32.73283100070343 34.0 31.0 34.0 31.0 34.0 3 32.83493539210013 34.0 31.0 34.0 31.0 34.0 4 36.27595144618434 37.0 37.0 37.0 35.0 37.0 5 36.24571099876294 37.0 37.0 37.0 35.0 37.0 6 36.29368440019821 37.0 37.0 37.0 35.0 37.0 7 36.24955212812774 37.0 37.0 37.0 35.0 37.0 8 36.23289683873494 37.0 37.0 37.0 35.0 37.0 9 37.98309348653959 39.0 38.0 39.0 35.0 39.0 10-11 37.900484949079484 39.0 38.0 39.0 35.0 39.0 12-13 37.81850707065807 39.0 38.0 39.0 35.0 39.0 14-15 39.220597254900596 40.5 39.0 41.0 36.0 41.0 16-17 39.317664170596736 41.0 39.0 41.0 36.0 41.0 18-19 39.36717870174333 41.0 39.0 41.0 36.0 41.0 20-21 39.33952499592844 41.0 39.0 41.0 36.0 41.0 22-23 39.25268896381334 41.0 39.0 41.0 36.0 41.0 24-25 39.18104159231012 41.0 39.0 41.0 36.0 41.0 26-27 39.030617283522815 40.5 39.0 41.0 35.0 41.0 28-29 38.87994954727691 40.0 38.0 41.0 35.0 41.0 30-31 38.67114509662598 40.0 38.0 41.0 35.0 41.0 32-33 38.53882538021463 40.0 38.0 41.0 35.0 41.0 34-35 38.407230748439815 40.0 38.0 41.0 34.0 41.0 36-37 38.24538960521437 40.0 38.0 41.0 34.0 41.0 38-39 38.10283987151188 40.0 38.0 41.0 34.0 41.0 40-41 37.917220803431896 40.0 38.0 41.0 33.0 41.0 42-43 37.71015672916659 40.0 37.0 41.0 33.0 41.0 44-45 37.42245492693711 40.0 37.0 41.0 33.0 41.0 46-47 37.05777547152158 40.0 37.0 41.0 31.5 41.0 48-49 36.66912750747608 40.0 36.5 41.0 31.0 41.0 50-51 36.389838246352056 40.0 36.0 41.0 30.0 41.0 52-53 36.13184239068288 39.0 35.5 41.0 30.0 41.0 54-55 35.878945517296344 39.0 35.0 40.0 29.0 41.0 56-57 35.640450713303096 39.0 35.0 40.0 28.0 41.0 58-59 35.30578473735824 38.0 35.0 40.0 28.0 41.0 60-61 34.91669843062924 38.0 34.5 40.0 26.5 41.0 62-63 34.519307869030825 37.5 34.0 40.0 26.0 41.0 64-65 34.05243479436011 37.0 34.0 40.0 25.5 41.0 66-67 33.62447892663218 36.5 33.0 39.0 24.5 40.5 68-69 33.132716477180196 36.0 33.0 39.0 23.0 40.0 70-71 32.61124288342857 35.0 32.0 38.5 22.0 40.0 72-73 32.019788659918845 35.0 31.5 37.5 20.0 39.5 74-75 31.46755917626227 35.0 31.0 37.0 18.0 39.0 76-77 29.47235409079411 32.5 28.5 35.0 13.0 37.5 78-79 30.449831419987735 34.0 30.0 36.0 13.0 38.0 80-81 30.43539903044836 34.0 31.0 35.5 10.0 37.0 82-83 30.042389296815173 34.0 30.0 35.0 7.0 37.0 84-85 29.555169671537527 34.0 30.0 35.0 2.0 36.5 86-87 28.84080537238337 34.0 29.0 35.0 2.0 36.0 88-89 28.15126374368908 33.5 28.0 35.0 2.0 35.5 90-91 27.5796501574915 33.0 26.5 35.0 2.0 35.0 92-93 27.123180358089588 33.0 26.0 35.0 2.0 35.0 94-95 26.56077629969472 32.5 25.0 34.0 2.0 35.0 96-97 25.741287722593185 32.0 23.5 34.0 2.0 35.0 98-99 24.53676014512088 32.0 10.5 34.0 2.0 35.0 100-101 18.728988138758847 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11243917538014614 1101 2 0.11232762193139223 1101 3 0.22090580481641808 1101 4 -0.006523992213068652 1101 5 0.0641884872098899 1101 6 -0.014722047919804027 1101 7 0.17547562622963397 1101 8 0.09488640771623125 1101 9 0.2081593523171108 1101 10-11 0.0509165678660608 1101 12-13 -0.017749203987392548 1101 14-15 0.17860273905439783 1101 16-17 0.1657111552731223 1101 18-19 0.1333514146201793 1101 20-21 0.05307642677369273 1101 22-23 0.03273517155101757 1101 24-25 0.005623483182866096 1101 26-27 0.14421278170343044 1101 28-29 0.1382102394661473 1101 30-31 0.08473368918179602 1101 32-33 0.18303833070046238 1101 34-35 0.10459306125653711 1101 36-37 0.24646332918167246 1101 38-39 0.1389893445137531 1101 40-41 0.2981769712957103 1101 42-43 0.21046787400617717 1101 44-45 0.15159085420506813 1101 46-47 0.19043477801476882 1101 48-49 0.32494161800796917 1101 50-51 0.36084714688391273 1101 52-53 0.301940918218925 1101 54-55 0.42190547528557687 1101 56-57 0.4447487373935104 1101 58-59 0.29606877232127005 1101 60-61 0.5123413409455253 1101 62-63 0.16385316078171996 1101 64-65 0.3110666353196905 1101 66-67 0.3140615498852455 1101 68-69 0.3164256691281935 1101 70-71 0.2434009932607708 1101 72-73 0.3531854129666101 1101 74-75 -0.20316282450794176 1101 76-77 0.08397183447912582 1101 78-79 0.16906137889111506 1101 80-81 0.3374687907127836 1101 82-83 0.06511113983096806 1101 84-85 0.19850198636875405 1101 86-87 0.28638898381998956 1101 88-89 -0.21509837895714767 1101 90-91 -0.13072052548770685 1101 92-93 -0.18704271301805164 1101 94-95 -0.04366656045134576 1101 96-97 0.13033229565724724 1101 98-99 -0.4579940630468009 1101 100-101 -0.5620429714697437 1102 1 0.19838813255081789 1102 2 0.10807822380432697 1102 3 0.21510980595759577 1102 4 0.06164155913257474 1102 5 0.07168624739168195 1102 6 0.06432977071248303 1102 7 0.17863389503785498 1102 8 0.18853833746351967 1102 9 0.13997378025001694 1102 10-11 0.20878496165363458 1102 12-13 0.2447174489984505 1102 14-15 0.2142721569018704 1102 16-17 0.3138895198804974 1102 18-19 0.08270149195021048 1102 20-21 0.2562116717522471 1102 22-23 0.23094031387331881 1102 24-25 0.36293830402243543 1102 26-27 0.2492364812324439 1102 28-29 0.29455705842377 1102 30-31 0.12955758191076683 1102 32-33 0.2782919181634824 1102 34-35 0.4021985829715078 1102 36-37 0.4488452945157988 1102 38-39 0.601953512427535 1102 40-41 0.6038933877867834 1102 42-43 0.6410635405210243 1102 44-45 0.4111494974007712 1102 46-47 0.48795020648324083 1102 48-49 0.6469974457897294 1102 50-51 0.46541787759018405 1102 52-53 0.23999680606284102 1102 54-55 0.43870035798917684 1102 56-57 0.4861716100667266 1102 58-59 0.3359625623940019 1102 60-61 0.1580946956676641 1102 62-63 0.24986968788727637 1102 64-65 0.25711579620120517 1102 66-67 0.5725165508112084 1102 68-69 0.6446653972467971 1102 70-71 0.46124896591246767 1102 72-73 0.738987217834655 1102 74-75 0.48187869346315537 1102 76-77 0.26570492823116254 1102 78-79 0.5439268646688973 1102 80-81 0.6620997837906195 1102 82-83 0.1661607261528104 1102 84-85 0.2578459073269457 1102 86-87 0.5859340129251152 1102 88-89 0.6978665397336989 1102 90-91 0.5347557424496081 1102 92-93 0.5164879412090748 1102 94-95 0.05279327547883739 1102 96-97 0.18444830572767046 1102 98-99 0.17260110295000075 1102 100-101 0.28608233822579265 1103 1 -0.13620545814033846 1103 2 -0.006505701755209259 1103 3 0.03304525803938674 1103 4 0.0617363153248931 1103 5 0.0488405294240124 1103 6 -0.017577481926480232 1103 7 0.14732644909626202 1103 8 -0.021853574147904453 1103 9 0.049026786864004634 1103 10-11 -0.06527837338531128 1103 12-13 0.021329725610719663 1103 14-15 -0.13158083834137102 1103 16-17 0.05559124633831658 1103 18-19 0.004991938630901416 1103 20-21 0.039013896627643874 1103 22-23 0.058045796466608124 1103 24-25 0.1663405251075858 1103 26-27 0.01791529564296468 1103 28-29 0.09649292743281279 1103 30-31 0.08016354776064105 1103 32-33 0.12881402478316062 1103 34-35 -0.1782588999249981 1103 36-37 -0.06487303322692384 1103 38-39 0.03667123373062253 1103 40-41 0.0030254210651534663 1103 42-43 0.039785753267629786 1103 44-45 0.2019444070981251 1103 46-47 -0.20753892498150606 1103 48-49 -0.4961910053255565 1103 50-51 -0.1259223539939427 1103 52-53 -0.4027652421870158 1103 54-55 -0.41386848238454377 1103 56-57 -0.35465220026927824 1103 58-59 -0.5387863336963647 1103 60-61 -0.09827055155123787 1103 62-63 -0.3860035851811219 1103 64-65 -0.242164056737181 1103 66-67 -0.15239674727282448 1103 68-69 -0.25277760690352125 1103 70-71 -0.39475715533755107 1103 72-73 -0.35290023076011323 1103 74-75 -0.3387549411948676 1103 76-77 -0.34710705653204244 1103 78-79 -0.46338831337799036 1103 80-81 -0.48645897452423625 1103 82-83 -0.3568081748648737 1103 84-85 -0.46186053798925286 1103 86-87 -0.25517880379745606 1103 88-89 -0.24456433562400548 1103 90-91 -0.18767968120532785 1103 92-93 -0.29206191002370474 1103 94-95 -0.3867262158699205 1103 96-97 -0.42785199559702036 1103 98-99 -0.9642813235665706 1103 100-101 -1.4364786373839635 1104 1 0.13839618702498768 1104 2 0.2248032931237276 1104 3 0.12745490777263058 1104 4 0.06621501917729233 1104 5 0.09386845441018465 1104 6 0.1281499059721014 1104 7 0.15441029542854068 1104 8 0.11485841486053516 1104 9 0.16665075417481745 1104 10-11 0.17178067056308777 1104 12-13 0.23418414097036333 1104 14-15 -0.11650982144362843 1104 16-17 0.1630556040636364 1104 18-19 0.28044114727426006 1104 20-21 0.20289475596779738 1104 22-23 0.1460072724761048 1104 24-25 0.10410037022651153 1104 26-27 0.3052683102924263 1104 28-29 0.3194333481811569 1104 30-31 0.09458773989882019 1104 32-33 -0.06890915206319193 1104 34-35 -0.06818270842917684 1104 36-37 0.013653723013824504 1104 38-39 -0.027846190390143022 1104 40-41 0.17907773803605664 1104 42-43 0.1340459641771048 1104 44-45 -0.17038353751583202 1104 46-47 -0.05474776462249764 1104 48-49 -0.17117048536766077 1104 50-51 -0.4533985776079561 1104 52-53 -0.44485569059270347 1104 54-55 -0.27154919183978166 1104 56-57 -0.3828760640881299 1104 58-59 -0.3203937601367741 1104 60-61 -0.5731358335668375 1104 62-63 -0.4231603219725102 1104 64-65 -0.3726353020309716 1104 66-67 -0.7062214889890015 1104 68-69 -0.7448216623609483 1104 70-71 -0.5000803000657115 1104 72-73 -0.48766059333884826 1104 74-75 -0.5381487102650446 1104 76-77 -0.5092905584858975 1104 78-79 -0.23377890631555331 1104 80-81 -0.09293079080816113 1104 82-83 -0.709251834197886 1104 84-85 -0.7624823819305107 1104 86-87 -0.9287954909840863 1104 88-89 -0.6642376222075228 1104 90-91 -0.8721942503754256 1104 92-93 -0.9854446696903878 1104 94-95 -0.8103362117659287 1104 96-97 -0.9963306637625386 1104 98-99 -1.1927688242356957 1104 100-101 -1.3647145455259242 1105 1 -0.11788557527862054 1105 2 -0.029124627760587884 1105 3 -0.057038849973004346 1105 4 -0.02972689167616238 1105 5 -0.2511730913067396 1105 6 -0.0950283621662038 1105 7 0.055216257384472556 1105 8 -0.1708047778799795 1105 9 -0.012794304837569825 1105 10-11 -0.08759934590548113 1105 12-13 -0.006550207647698869 1105 14-15 0.032842664229541185 1105 16-17 -0.08680923719762035 1105 18-19 -0.01810371295080415 1105 20-21 0.035204348495391 1105 22-23 -0.023529611304020648 1105 24-25 0.005322205224722154 1105 26-27 0.07837005779094852 1105 28-29 0.044393632305620656 1105 30-31 -0.05540103152903697 1105 32-33 0.03951310878901637 1105 34-35 -0.07382194688246102 1105 36-37 -0.21203225956941907 1105 38-39 -0.37744154592 1105 40-41 -0.1572990928450153 1105 42-43 -0.20195242223548604 1105 44-45 -0.2934798786648116 1105 46-47 -0.3695532608006218 1105 48-49 -0.2743901744609829 1105 50-51 -0.28904763066503847 1105 52-53 -0.130397448404878 1105 54-55 -0.4288427732552478 1105 56-57 -0.3803953813909473 1105 58-59 -0.4442116410141068 1105 60-61 -0.3686718523310333 1105 62-63 -0.13611851009684273 1105 64-65 -0.246846182933254 1105 66-67 -0.06249992430826978 1105 68-69 -0.6013704151576889 1105 70-71 -0.545389668021862 1105 72-73 -0.8327458057252102 1105 74-75 -0.13100900187522058 1105 76-77 -0.5219206227798701 1105 78-79 -0.5343012428566567 1105 80-81 -0.16673785898638727 1105 82-83 -0.10724233565759889 1105 84-85 -0.44428128236219067 1105 86-87 -0.4188914744822405 1105 88-89 -0.4907249921432282 1105 90-91 -0.5554819762984593 1105 92-93 -0.8434801686132758 1105 94-95 -1.1505640270184863 1105 96-97 -1.685411501164495 1105 98-99 -1.3905122971082982 1105 100-101 -1.8104826214477114 1106 1 -0.0718250754043126 1106 2 -0.03712703495780545 1106 3 -0.012631393755810905 1106 4 0.0038519859300478743 1106 5 0.05618638093812223 1106 6 0.09335386418651126 1106 7 0.03636241843815924 1106 8 0.042056968252126126 1106 9 0.10965661804832649 1106 10-11 0.022209982745494017 1106 12-13 0.07254640780254817 1106 14-15 0.010092365234321221 1106 16-17 0.09742133573055156 1106 18-19 -0.007052316243182588 1106 20-21 -0.04225826757950557 1106 22-23 -0.026719528499484113 1106 24-25 0.049581116044159046 1106 26-27 -0.05720808851484094 1106 28-29 0.2808950453913326 1106 30-31 0.23895119627103867 1106 32-33 0.11629584400493798 1106 34-35 0.002682741819704404 1106 36-37 -0.08385662558527684 1106 38-39 0.2364432590284622 1106 40-41 0.23370476933532558 1106 42-43 0.22956243611314875 1106 44-45 0.14319598004185252 1106 46-47 -0.03271133595698217 1106 48-49 0.15375955929996366 1106 50-51 0.13735574245814774 1106 52-53 0.05840756100300126 1106 54-55 -0.08240633925891672 1106 56-57 -0.25158557282406235 1106 58-59 -0.1993021775296029 1106 60-61 -0.4449289653702664 1106 62-63 -0.16418787698873416 1106 64-65 -0.04518247447835222 1106 66-67 -0.25165153964425 1106 68-69 -0.26510172319514425 1106 70-71 -0.17917193855731028 1106 72-73 -0.0942980670432263 1106 74-75 -0.21456388368640944 1106 76-77 0.0038881878666430225 1106 78-79 0.01063564439358089 1106 80-81 0.0651662644815012 1106 82-83 -0.11799854599788162 1106 84-85 -0.07847490938373625 1106 86-87 -0.6085477632849674 1106 88-89 -0.6702192521967056 1106 90-91 -0.27833435264393813 1106 92-93 -0.35162540792372 1106 94-95 -0.3237165412445684 1106 96-97 -1.1353212312689358 1106 98-99 -1.3519057291164067 1106 100-101 -1.2321287329862578 1107 1 0.05781163587676019 1107 2 0.007767802518294786 1107 3 0.020346845340974085 1107 4 -0.09250055891198627 1107 5 -0.02883956825608891 1107 6 0.021348036999036424 1107 7 0.016142077911887043 1107 8 -0.008581290875618208 1107 9 -0.11937370153648885 1107 10-11 0.1014615533546177 1107 12-13 0.049998834279833204 1107 14-15 0.04434712258659346 1107 16-17 0.06712360287067298 1107 18-19 0.02284297648193956 1107 20-21 0.04696871958246618 1107 22-23 0.008454831875646107 1107 24-25 0.1237703175373781 1107 26-27 0.03801908074674998 1107 28-29 0.0515478724877525 1107 30-31 0.21632349892058755 1107 32-33 0.2822450382141639 1107 34-35 0.11722339555730343 1107 36-37 0.09824662489273805 1107 38-39 0.18264192970132598 1107 40-41 0.34341614145588295 1107 42-43 0.36387000235188083 1107 44-45 0.29801888817260647 1107 46-47 0.40512896268638343 1107 48-49 0.09905319491864617 1107 50-51 0.1666173285457404 1107 52-53 0.16698445256268712 1107 54-55 0.21240746389140241 1107 56-57 0.23901080339372527 1107 58-59 0.07615261624258096 1107 60-61 0.31597242769173306 1107 62-63 0.1467253525478185 1107 64-65 -0.029221443794568813 1107 66-67 0.026724131848062882 1107 68-69 -0.2600120391379619 1107 70-71 -0.41434811992851905 1107 72-73 -0.48949974235962657 1107 74-75 -0.3985901060300314 1107 76-77 -0.20772976715475622 1107 78-79 -0.08658733657701134 1107 80-81 0.09354102547576204 1107 82-83 -0.3189327169894156 1107 84-85 -0.34348446961318757 1107 86-87 0.056811428192773405 1107 88-89 0.18663224557257507 1107 90-91 0.4877659842403368 1107 92-93 0.49080744284565014 1107 94-95 0.2837804996367943 1107 96-97 0.32874141099638265 1107 98-99 0.19287984859460394 1107 100-101 -1.258145304050629 1108 1 0.05635178186216194 1108 2 0.09083349594895651 1108 3 0.14963082170524444 1108 4 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-0.6076561586388145 2316 78-79 -0.6221685607432548 2316 80-81 -0.567315098186544 2316 82-83 -0.5276060650444521 2316 84-85 -0.5852204981506404 2316 86-87 -0.6081214748138493 2316 88-89 -0.9524085788428032 2316 90-91 -1.012905551912766 2316 92-93 -1.0649640310233295 2316 94-95 -0.857229635689631 2316 96-97 -0.9835625355525082 2316 98-99 -0.6248235657080983 2316 100-101 0.1831705897266218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 11.0 6 27.0 7 80.0 8 150.0 9 275.0 10 469.0 11 805.0 12 1324.0 13 1913.0 14 2230.0 15 2872.0 16 3901.0 17 5840.0 18 5875.0 19 3873.0 20 3378.0 21 3505.0 22 3804.0 23 4171.0 24 4447.0 25 5202.0 26 6297.0 27 7203.0 28 8805.0 29 10756.0 30 12944.0 31 15877.0 32 20113.0 33 25801.0 34 36268.0 35 54944.0 36 88099.0 37 138100.0 38 93005.0 39 4806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88312312061477 19.516023499276088 14.699924824590713 21.90092855551843 2 15.2470485503505 21.251996798192575 40.55449483171453 22.9464598197424 3 19.650746568625753 26.356350078139346 29.83848891322201 24.15441444001289 4 12.737233485915858 17.397526569110873 37.51537664551765 32.34986329945562 5 13.220623243597252 37.97052535283987 33.85703444715112 14.95181695641176 6 29.94972053488203 36.46335420514438 18.48679947468181 15.100125785291784 7 27.555641799526658 33.339339609892335 21.388004310658484 17.71701427992252 8 25.82877953615374 34.750872353917536 20.114731432808824 19.305616677119897 9 26.526489412204985 17.4954173264908 21.196034471407234 34.78205878989698 10-11 24.694979330323264 27.055186131045406 27.239186103324126 21.010648435307203 12-13 26.05548759992654 24.669423778617887 27.90830841306088 21.366780208394694 14-15 22.822164122342407 25.773996087787417 27.113123238161574 24.290716551708606 16-17 21.30158958423393 28.99767570194581 27.695618312661836 22.00511640115842 18-19 21.163047159996083 27.617692080457612 30.300767446876893 20.918493312669415 20-21 22.41048626584011 26.443412211915295 30.05471486934616 21.091386652898432 22-23 21.82816620291281 26.45328791664212 30.06614989587196 21.652395984573108 24-25 21.784177547998997 26.684437175303437 30.20738131601676 21.324003960680802 26-27 21.554448583557136 26.561119293078058 30.556614398336656 21.327817725028154 28-29 21.104572541878657 27.132049548359376 30.511795846482904 21.251582063279066 30-31 21.471254768925835 26.746353785860073 30.240967195334512 21.541424249879583 32-33 21.351638632057778 26.901529609380887 30.205735363803086 21.54109639475825 34-35 21.954023685608163 26.784629740175685 30.00745969296562 21.253886881250526 36-37 21.9011637479095 26.56308761470672 30.1228737554483 21.41287488193548 38-39 21.59465358424911 27.01055956968042 30.165780131858156 21.22900671421231 40-41 21.64225978578316 26.95072388934469 30.070842477069547 21.336173847802602 42-43 21.491567772308194 27.043166975955202 29.516262339053007 21.949002912683603 44-45 21.598710912627077 26.992692572586968 29.473925868812834 21.934670645973117 46-47 21.993790143585603 27.384791049767216 28.88553522759366 21.735883579053517 48-49 21.893070782238816 27.297661383591134 28.96938877169577 21.83987906247428 50-51 21.634180570715735 27.668018088258222 29.016494273784154 21.681307067241885 52-53 21.897296136000584 27.895227435747277 28.455283848544806 21.752192579707334 54-55 21.54298357167856 27.568896034817925 28.72392034291219 22.16420005059133 56-57 21.774618311352594 27.698848870007552 28.54530365298386 21.98122916565599 58-59 22.048701802169397 27.2919119386034 28.630003006070183 22.02938325315702 60-61 21.842480967215444 27.670394100789725 28.953309469438388 21.53381546255644 62-63 21.628100183050382 27.589322609624485 28.93738082882927 21.845196378495867 64-65 21.36619080167614 27.763122672328876 28.81066672211061 22.060019803884366 66-67 21.474114630258505 27.643547716404466 28.398364405017674 22.483973248319355 68-69 21.3691414710214 27.686909815472582 28.63510352594646 22.30884518755956 70-71 21.71763505301284 27.54995698852074 28.407501063375445 22.324906895090972 72-73 21.63160502725348 27.449607917196545 28.53195050366094 22.38683655188903 74-75 21.362881903897264 28.07662853836105 28.12886581862662 22.431623739115068 76-77 21.41472809399147 27.527919868957202 28.415441753503913 22.641910283547418 78-79 21.55418677170239 27.499703244040834 28.490651537455804 22.45545844680097 80-81 21.64956312773857 27.691793246715445 28.21105010473499 22.44759352081099 82-83 21.558836676634776 27.553129558850536 28.36700890892521 22.521024855589474 84-85 21.406456285279656 27.581977012131524 28.294587074261834 22.716979628326985 86-87 21.24525192958113 27.580088457202322 28.41323389124967 22.761425721966873 88-89 21.32529166699289 27.472764553336937 28.18532717768307 23.016616601987103 90-91 21.243510244418463 27.377025221312845 28.343877011302265 23.035587522966427 92-93 21.42343556709069 27.459743508889865 28.195050366094105 22.92177055792534 94-95 21.128203973151944 27.21215785880861 28.382688755903768 23.276949412135682 96-97 21.01705898048076 27.130553351329063 28.495652957340422 23.35673471084976 98-99 21.08659023082354 27.267238130714823 27.95931892111977 23.686852717341868 100-101 21.297204299584873 26.054365076614943 28.892253955493334 23.756176668306846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 393.0 1 285.5 2 358.0 3 465.5 4 307.0 5 227.5 6 344.0 7 636.0 8 972.0 9 1118.0 10 970.0 11 829.0 12 886.0 13 934.5 14 956.5 15 1062.5 16 1133.0 17 1132.0 18 1179.0 19 1281.0 20 1407.0 21 1587.0 22 1876.0 23 2212.5 24 2583.5 25 3039.0 26 3794.5 27 4624.0 28 5343.5 29 6140.0 30 7144.5 31 8355.0 32 9611.0 33 10745.5 34 11996.5 35 13243.5 36 14645.5 37 16260.0 38 17070.0 39 17266.5 40 17333.0 41 17646.0 42 18140.0 43 18783.0 44 19553.0 45 20148.0 46 20402.5 47 21619.0 48 25014.0 49 24902.0 50 21667.5 51 19780.5 52 18417.0 53 17840.5 54 17248.0 55 16003.5 56 14324.0 57 12769.5 58 11688.0 59 10280.5 60 8469.0 61 6916.5 62 5662.5 63 4478.5 64 3365.0 65 2511.5 66 1914.0 67 1551.0 68 1189.5 69 842.0 70 636.5 71 472.0 72 352.0 73 317.5 74 253.0 75 178.0 76 129.0 77 70.0 78 40.0 79 23.0 80 10.5 81 6.5 82 2.5 83 1.5 84 1.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4362635877568983 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012128058436450706 16-17 0.001819208765467606 18-19 8.662898883179075E-5 20-21 0.0 22-23 0.0 24-25 8.662898883179075E-5 26-27 0.004158191463925957 28-29 8.662898883179075E-5 30-31 0.0 32-33 8.662898883179075E-5 34-35 0.012561203380609662 36-37 0.027548018448509463 38-39 0.019838038442480083 40-41 0.008403011916683703 42-43 0.007450093039534006 44-45 0.003725046519767003 46-47 0.004677965396916701 48-49 0.002858756631449095 50-51 6.93031910654326E-4 52-53 0.0 54-55 0.0 56-57 3.46515955327163E-4 58-59 0.0014726928101404428 60-61 0.0020790957319629785 62-63 0.001819208765467606 64-65 0.002512240676121932 66-67 0.0024256116872901413 68-69 0.004158191463925957 70-71 4.331449441589538E-4 72-73 0.0 74-75 0.0 76-77 0.09849716030174609 78-79 0.017412426755189944 80-81 5.197739329907446E-4 82-83 0.0 84-85 0.0 86-87 0.10811297806207487 88-89 0.4188511610017083 90-91 0.41963082190119444 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.598869664953723E-4 100-101 3.46515955327163E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 577174.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.69982331854746 #Duplication Level Percentage of deduplicated Percentage of total 1 86.44712286433177 61.1179631290502 2 8.52257424221637 12.050889862878016 3 2.135354089313472 4.529074705110008 4 0.8971715106135935 2.537194691472615 5 0.4943526654954386 1.7475323053790248 6 0.3070297538379846 1.3024169609929577 7 0.22605238616016915 1.1187304633582016 8 0.15120789759906159 0.8552297315698136 9 0.11721896280802273 0.7458623964099533 >10 0.653619568225936 8.264949444855949 >50 0.032360821190469034 1.5843734598290096 >100 0.013238505587946352 1.8240820562698108 >500 0.0014709450653217744 0.6522254634862568 >1k 0.001225787554434812 1.6694753293381943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 0.4723012471109232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.4059434416657715 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1979 0.34287753779622787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1493 0.2586741606517272 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1091 0.18902445363096743 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 768 0.13306212684563062 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.11348397536964588 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.11313745941431873 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 607 0.10516759244179397 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.662898883179077E-5 0.0 18-19 0.0 0.0 0.0 1.7325797766358153E-4 0.0 20-21 0.0 0.0 0.0 1.7325797766358153E-4 0.0 22-23 0.0 0.0 0.0 6.064029218225353E-4 0.0 24-25 1.7325797766358153E-4 0.0 0.0 0.0012128058436450706 0.0 26-27 1.7325797766358153E-4 0.0 0.0 0.0023389826984583505 0.0 28-29 1.7325797766358153E-4 0.0 0.0 0.005111110341075655 0.0 30-31 1.7325797766358153E-4 0.0 0.0 0.01134839753696459 0.0 32-33 1.7325797766358153E-4 0.0 0.0 0.021397360241452317 0.0 34-35 1.7325797766358153E-4 0.0 0.0 0.03638417530935212 0.0 36-37 2.598869664953723E-4 0.0 0.0 0.05916759937211309 0.0 38-39 5.197739329907445E-4 0.0 0.0 0.10152917491085878 0.0 40-41 5.197739329907445E-4 0.0 0.0 0.14683613606988533 0.0 42-43 5.197739329907445E-4 0.0 0.0 0.1948285958826974 0.0 44-45 6.930319106543261E-4 0.0 0.0 0.2519170995228475 0.0 46-47 6.930319106543261E-4 0.0 0.0 0.324165676208561 0.0 48-49 6.930319106543261E-4 0.0 0.0 0.4003125573917051 0.0 50-51 6.930319106543261E-4 0.0 0.0 0.4875479491453184 1.7325797766358153E-4 52-53 6.930319106543261E-4 0.0 0.0 0.5779019844968762 1.7325797766358153E-4 54-55 6.930319106543261E-4 0.0 0.0 0.6743200490666592 1.7325797766358153E-4 56-57 6.930319106543261E-4 0.0 0.0 0.7888435723022866 1.7325797766358153E-4 58-59 6.930319106543261E-4 0.0 0.0 0.9254574876900207 1.7325797766358153E-4 60-61 6.930319106543261E-4 0.0 0.0 1.075239009380187 1.7325797766358153E-4 62-63 6.930319106543261E-4 0.0 0.0 1.2244141281485306 1.7325797766358153E-4 64-65 6.930319106543261E-4 0.0 0.0 1.3860638213086522 1.7325797766358153E-4 66-67 8.662898883179076E-4 0.0 0.0 1.57318243718532 1.7325797766358153E-4 68-69 8.662898883179076E-4 0.0 0.0 1.7901014252201244 1.7325797766358153E-4 70-71 9.529188771496983E-4 0.0 0.0 2.0361277535024103 1.7325797766358153E-4 72-73 0.001039547865981489 0.0 0.0 2.290297206734884 1.7325797766358153E-4 74-75 0.001039547865981489 0.0 0.0 2.5475853035653024 1.7325797766358153E-4 76-77 0.001039547865981489 0.0 0.0 2.8319882739000715 1.7325797766358153E-4 78-79 0.001039547865981489 0.0 0.0 3.1558074341533056 1.7325797766358153E-4 80-81 0.0013860638213086523 0.0 0.0 3.50189024453631 1.7325797766358153E-4 82-83 0.0013860638213086523 0.0 0.0 3.869803560104925 1.7325797766358153E-4 84-85 0.0015593217989722337 0.0 0.0 4.249844934109991 1.7325797766358153E-4 86-87 0.0015593217989722337 0.0 0.0 4.652669732177818 1.7325797766358153E-4 88-89 0.0015593217989722337 0.0 0.0 5.077758180375415 1.7325797766358153E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 745 0.0 33.126667 2 GTAAGCG 45 2.4267934E-5 31.640215 94-95 CTCGTAG 80 4.406355E-4 29.783974 1 GTACCTG 890 0.0 28.91388 1 TCTAGAC 150 3.3576725E-8 28.476192 3 ACCTGGG 985 0.0 23.609705 3 GTACATG 3300 0.0 22.960735 1 TACATGG 3290 0.0 22.359728 2 ACATGGG 3255 0.0 22.016922 3 GAGTACT 2610 0.0 21.004375 12-13 GTATCAA 5560 0.0 20.741644 1 ATACGGA 115 0.0037391244 20.634922 9 ATAGCCC 140 4.8751064E-4 20.34014 3 GTATAGC 260 1.4317266E-8 20.16146 1 CATGGGG 1370 0.0 20.09269 4 AGTACTT 2720 0.0 20.067684 12-13 TAGCCCT 335 3.45608E-11 19.834166 4 GGTATCA 4435 0.0 19.663467 1 GTATAGA 170 7.749412E-5 19.622383 1 GTACTTT 2830 0.0 19.11995 14-15 >>END_MODULE