FastQCFastQC Report
Fri 27 May 2016
SRR939223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574527
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57850.22470146943496805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.18900559209516932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.11260320827864692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.031.7011721
GTATCAA91500.029.5756071
ATCAACG107100.024.7751393
TCAACGC107550.024.6714764
CAACGCA108350.024.5330475
TATCAAC110150.024.2181782
AACGCAG111750.023.9040666
TACATGG68550.022.533452
GTACATG71850.022.3341711
ACGCAGA122150.021.674977
CGCAGAG123700.021.3646748
GAGTACT69300.021.18634412-13
ACATGGG73300.020.4268153
CGCGGAA3150.020.37691182-83
AGAGTAC102650.019.6081410-11
GTACTTT73650.019.4220714-15
GCAGAGT140750.018.3381089
AGTACTT74250.017.92410312-13
CATGGGG49750.017.3338834
GTATAGT7850.016.934341
Produced by FastQC (version 0.11.5)